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  1. Article ; Online: A gut feeling: diet-sensing mesenchymal cells regulate intestinal stem cell function.

    Messina-Pacheco, Julia / Gregorieff, Alex

    Cell research

    2022  Volume 32, Issue 7, Page(s) 605–606

    MeSH term(s) Diet ; Intestinal Mucosa ; Mesenchymal Stem Cells ; Stem Cells/physiology
    Language English
    Publishing date 2022-04-06
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 1319303-x
    ISSN 1748-7838 ; 1001-0602
    ISSN (online) 1748-7838
    ISSN 1001-0602
    DOI 10.1038/s41422-022-00658-2
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  2. Article ; Online: Dangerous liaisons: how helminths manipulate the intestinal epithelium.

    Karo-Atar, Danielle / Gregorieff, Alex / King, Irah L

    Trends in parasitology

    2023  Volume 39, Issue 6, Page(s) 414–422

    Abstract: Intestinal helminths remain highly pervasive throughout the animal kingdom by modulating multiple aspects of the host immune response. The intestinal epithelium functions as a physical barrier as well as a sentinel innate immune tissue with the ability ... ...

    Abstract Intestinal helminths remain highly pervasive throughout the animal kingdom by modulating multiple aspects of the host immune response. The intestinal epithelium functions as a physical barrier as well as a sentinel innate immune tissue with the ability to sense and respond to infectious agents. Although helminths form intimate interactions with the epithelium, comprehensive knowledge about host-helminth interactions at this dynamic interface is lacking. In addition, little is known about the ability of helminths to directly shape the fate of this barrier tissue. Here, we review the diverse pathways by which helminths regulate the epithelium and highlight the emerging field of direct helminth regulation of intestinal stem cell (ISC) fate and function.
    MeSH term(s) Animals ; Intestinal Mucosa ; Helminths ; Intestines/parasitology ; Helminthiasis ; Intestinal Diseases, Parasitic
    Language English
    Publishing date 2023-04-17
    Publishing country England
    Document type Journal Article ; Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 2036227-4
    ISSN 1471-5007 ; 1471-4922
    ISSN (online) 1471-5007
    ISSN 1471-4922
    DOI 10.1016/j.pt.2023.03.012
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  3. Article ; Online: Multiplex In Situ Hybridization in the Study of Acute Kidney Injury : Multiplex In Situ Hybridization in AKI.

    Masztalerz, Agnieszka / Gregorieff, Alex / Lemay, Serge / Takano, Tomoko

    Methods in molecular biology (Clifton, N.J.)

    2023  Volume 2664, Page(s) 217–232

    Abstract: Recently developed in situ hybridization (ISH) methods, such as RNAscope™, have greatly expanded the accessibility and usefulness of ISH in biomedical research. Among many other advantages over traditional ISH, these newer methods enable the simultaneous ...

    Abstract Recently developed in situ hybridization (ISH) methods, such as RNAscope™, have greatly expanded the accessibility and usefulness of ISH in biomedical research. Among many other advantages over traditional ISH, these newer methods enable the simultaneous use of multiple probes, including combination with antibody or lectin staining. We herein illustrate the application of RNAscope™ multiplex ISH in the study of the adapter protein Dok-4 in acute kidney injury (AKI). Specifically, we used multiplex ISH to define the expression of Dok-4 and some of its putative binding partners, together with nephron segment markers, as well as markers of proliferation and tubular injury. We also illustrate the use of QuPath image analysis software to perform quantitative analyses of multiplex ISH. Furthermore, we describe how these analyses can exploit the uncoupling of mRNA and protein expression in a knockout (KO) mouse created by CRISPR/CAS9-mediated frame shift to carry out highly focused molecular phenotyping studies at the single-cell level.
    MeSH term(s) Mice ; Animals ; In Situ Hybridization ; Acute Kidney Injury/genetics ; Acute Kidney Injury/metabolism ; Nephrons/metabolism ; RNA, Messenger/genetics ; Staining and Labeling ; Kidney/metabolism ; Reperfusion Injury/metabolism
    Chemical Substances RNA, Messenger
    Language English
    Publishing date 2023-07-10
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-0716-3179-9_16
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  4. Article ; Online: Taking a Step Back: Insights into the Mechanisms Regulating Gut Epithelial Dedifferentiation.

    Ouladan, Shaida / Gregorieff, Alex

    International journal of molecular sciences

    2021  Volume 22, Issue 13

    Abstract: Despite the environmental constraints imposed upon the intestinal epithelium, this tissue must perform essential functions such as nutrient absorption and hormonal regulation, while also acting as a critical barrier to the outside world. These functions ... ...

    Abstract Despite the environmental constraints imposed upon the intestinal epithelium, this tissue must perform essential functions such as nutrient absorption and hormonal regulation, while also acting as a critical barrier to the outside world. These functions depend on a variety of specialized cell types that are constantly renewed by a rapidly proliferating population of intestinal stem cells (ISCs) residing at the base of the crypts of Lieberkühn. The niche components and signals regulating crypt morphogenesis and maintenance of homeostatic ISCs have been intensely studied over the last decades. Increasingly, however, researchers are turning their attention to unraveling the mechanisms driving gut epithelial regeneration due to physical damage or infection. It is now well established that injury to the gut barrier triggers major cell fate changes, demonstrating the highly plastic nature of the gut epithelium. In particular, lineage tracing and transcriptional profiling experiments have uncovered several injury-induced stem-cell populations and molecular markers of the regenerative state. Despite the progress achieved in recent years, several questions remain unresolved, particularly regarding the mechanisms driving dedifferentiation of the gut epithelium. In this review, we summarize the latest studies, primarily from murine models, that define the regenerative processes governing the gut epithelium and discuss areas that will require more in-depth investigation.
    MeSH term(s) Animals ; Cell Differentiation ; Gene Expression Regulation ; Gene Regulatory Networks ; Homeostasis ; Humans ; Intestinal Mucosa/cytology ; Intestinal Mucosa/physiology ; Regeneration ; Stem Cell Niche
    Language English
    Publishing date 2021-06-30
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms22137043
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  5. Article ; Online: Taking a Step Back

    Shaida Ouladan / Alex Gregorieff

    International Journal of Molecular Sciences, Vol 22, Iss 7043, p

    Insights into the Mechanisms Regulating Gut Epithelial Dedifferentiation

    2021  Volume 7043

    Abstract: Despite the environmental constraints imposed upon the intestinal epithelium, this tissue must perform essential functions such as nutrient absorption and hormonal regulation, while also acting as a critical barrier to the outside world. These functions ... ...

    Abstract Despite the environmental constraints imposed upon the intestinal epithelium, this tissue must perform essential functions such as nutrient absorption and hormonal regulation, while also acting as a critical barrier to the outside world. These functions depend on a variety of specialized cell types that are constantly renewed by a rapidly proliferating population of intestinal stem cells (ISCs) residing at the base of the crypts of Lieberkühn. The niche components and signals regulating crypt morphogenesis and maintenance of homeostatic ISCs have been intensely studied over the last decades. Increasingly, however, researchers are turning their attention to unraveling the mechanisms driving gut epithelial regeneration due to physical damage or infection. It is now well established that injury to the gut barrier triggers major cell fate changes, demonstrating the highly plastic nature of the gut epithelium. In particular, lineage tracing and transcriptional profiling experiments have uncovered several injury-induced stem-cell populations and molecular markers of the regenerative state. Despite the progress achieved in recent years, several questions remain unresolved, particularly regarding the mechanisms driving dedifferentiation of the gut epithelium. In this review, we summarize the latest studies, primarily from murine models, that define the regenerative processes governing the gut epithelium and discuss areas that will require more in-depth investigation.
    Keywords intestinal stem cells ; fetal reprogramming ; dedifferentiation ; lineage tracing ; organoids ; Hippo signaling ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Dangerous liaisons: how helminths manipulate the intestinal epithelium

    Karo-Atar, Danielle / Gregorieff, Alex / King, Irah L.

    Trends in Parasitology. 2023 Apr. 17,

    2023  

    Abstract: Intestinal helminths remain highly pervasive throughout the animal kingdom by modulating multiple aspects of the host immune response. The intestinal epithelium functions as a physical barrier as well as a sentinel innate immune tissue with the ability ... ...

    Abstract Intestinal helminths remain highly pervasive throughout the animal kingdom by modulating multiple aspects of the host immune response. The intestinal epithelium functions as a physical barrier as well as a sentinel innate immune tissue with the ability to sense and respond to infectious agents. Although helminths form intimate interactions with the epithelium, comprehensive knowledge about host–helminth interactions at this dynamic interface is lacking. In addition, little is known about the ability of helminths to directly shape the fate of this barrier tissue. Here, we review the diverse pathways by which helminths regulate the epithelium and highlight the emerging field of direct helminth regulation of intestinal stem cell (ISC) fate and function.
    Keywords animals ; helminths ; immune response ; intestinal mucosa ; parasitology ; stem cells ; intestinal epithelium ; epithelial regeneration ; intestinal organoids ; host defense
    Language English
    Dates of publication 2023-0417
    Publishing place Elsevier Ltd
    Document type Article ; Online
    Note Pre-press version
    ZDB-ID 2036227-4
    ISSN 1471-5007 ; 1471-4922
    ISSN (online) 1471-5007
    ISSN 1471-4922
    DOI 10.1016/j.pt.2023.03.012
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Hippo signalling in intestinal regeneration and cancer.

    Gregorieff, Alex / Wrana, Jeffrey L

    Current opinion in cell biology

    2017  Volume 48, Page(s) 17–25

    Abstract: The Hippo pathway is a unique signalling module that regulates cell-specific transcriptional responses and responds to a wide range of intrinsic and extrinsic cues. Besides its classical role in restricting tissue size during development, Hippo ... ...

    Abstract The Hippo pathway is a unique signalling module that regulates cell-specific transcriptional responses and responds to a wide range of intrinsic and extrinsic cues. Besides its classical role in restricting tissue size during development, Hippo signalling is now recognized to control numerous processes including cell proliferation, survival, cell fate determination, epithelial-to-mesenchymal transitions and cell migration. Because of its highly dynamic nature, the intestinal epithelium has served as an exceptional model to study the complex roles of Hippo signalling. In this review, we shall present an overview of Hippo function in the mammalian intestine and discuss the various mechanisms regulating Hippo signalling and how they contribute to intestinal regeneration and cancer.
    Language English
    Publishing date 2017-10
    Publishing country England
    Document type Journal Article ; Review
    ZDB-ID 1026381-0
    ISSN 1879-0410 ; 0955-0674
    ISSN (online) 1879-0410
    ISSN 0955-0674
    DOI 10.1016/j.ceb.2017.04.005
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  8. Article: Multiple roles for the hippo effector yap in gut regeneration and cancer initiation.

    Gregorieff, Alex / Wrana, Jeffrey L

    Molecular & cellular oncology

    2016  Volume 3, Issue 3, Page(s) e1143992

    Abstract: The Hippo signaling effector Yes-associated protein (Yap) is known for its potent control of tissue growth. Our recent work now shows that Yap promotes regeneration in the intestine by reprogramming intestinal stem cells and blocking their terminal ... ...

    Abstract The Hippo signaling effector Yes-associated protein (Yap) is known for its potent control of tissue growth. Our recent work now shows that Yap promotes regeneration in the intestine by reprogramming intestinal stem cells and blocking their terminal differentiation. Similarly, in tumor-initiating cells Yap regenerative signaling synergizes with Wnt activation to drive adenoma formation.
    Language English
    Publishing date 2016-03-16
    Publishing country United States
    Document type Journal Article
    ISSN 2372-3556
    ISSN 2372-3556
    DOI 10.1080/23723556.2016.1143992
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  9. Article ; Online: Seeing is believing: Wnt3 localization in the gut epithelium.

    Gregorieff, Alex / Wrana, Jeffrey L

    Cell research

    2016  Volume 26, Issue 5, Page(s) 515–516

    Abstract: Wnt morphogens are notoriously elusive proteins. Thanks to a recent study published in Nature, Clevers and colleagues give us a first glimpse of a mammalian Wnt in action in the gut epithelium. ...

    Abstract Wnt morphogens are notoriously elusive proteins. Thanks to a recent study published in Nature, Clevers and colleagues give us a first glimpse of a mammalian Wnt in action in the gut epithelium.
    MeSH term(s) Animals ; Intestines ; Proteins ; Wnt3 Protein
    Chemical Substances Proteins ; Wnt3 Protein
    Language English
    Publishing date 2016-03-25
    Publishing country England
    Document type Journal Article ; Comment
    ZDB-ID 1319303-x
    ISSN 1748-7838 ; 1001-0602
    ISSN (online) 1748-7838
    ISSN 1001-0602
    DOI 10.1038/cr.2016.41
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  10. Article ; Online: Use of Organoids to Characterize Signaling Pathways in Cancer Initiation.

    Oatway, Christina / Hirsch, Calley L / Gregorieff, Alex

    Methods in molecular biology (Clifton, N.J.)

    2018  Volume 1765, Page(s) 315–331

    Abstract: The development of intestinal organoid technology has greatly accelerated research in the field of colorectal cancer. Contrary to traditional cancer cell lines, organoids are composed of multiple cell types arranged in 3D structures highly reminiscent of ...

    Abstract The development of intestinal organoid technology has greatly accelerated research in the field of colorectal cancer. Contrary to traditional cancer cell lines, organoids are composed of multiple cell types arranged in 3D structures highly reminiscent of their native tissues. Thus, organoids provide a near-physiological and readily accessible model to study tissue morphogenesis, adult stem cell behavior and tumorigenesis. Here, we provide protocols for establishing intestinal organoid cultures from genetically modified mouse lines and describe methods to overexpress and knockout genes of interest using lentiviral-based approaches.
    MeSH term(s) Adenomatous Polyposis Coli Protein/genetics ; Animals ; Cell Transformation, Neoplastic/pathology ; Colon/pathology ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/pathology ; Gene Knockout Techniques/instrumentation ; Gene Knockout Techniques/methods ; Genetic Vectors/genetics ; Lentivirus/genetics ; Mice ; Mice, Transgenic ; Neoplasms, Experimental/genetics ; Neoplasms, Experimental/pathology ; Organoids/pathology ; Rectum/pathology ; Signal Transduction ; Tissue Culture Techniques/instrumentation ; Tissue Culture Techniques/methods
    Chemical Substances Adenomatous Polyposis Coli Protein ; adenomatous polyposis coli protein, mouse
    Language English
    Publishing date 2018-03-27
    Publishing country United States
    Document type Journal Article
    ISSN 1940-6029
    ISSN (online) 1940-6029
    DOI 10.1007/978-1-4939-7765-9_21
    Database MEDical Literature Analysis and Retrieval System OnLINE

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