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  1. Article ; Online: Wingless Signaling: A Genetic Journey from Morphogenesis to Metastasis.

    Bejsovec, Amy

    Genetics

    2018  Volume 208, Issue 4, Page(s) 1311–1336

    Abstract: This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of ... ...

    Abstract This FlyBook chapter summarizes the history and the current state of our understanding of the Wingless signaling pathway. Wingless, the fly homolog of the mammalian Wnt oncoproteins, plays a central role in pattern generation during development. Much of what we know about the pathway was learned from genetic and molecular experiments in
    MeSH term(s) Animals ; Biomarkers ; Body Patterning/genetics ; Drosophila/embryology ; Drosophila/physiology ; Drosophila Proteins/chemistry ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Evolution, Molecular ; Genetic Association Studies ; Humans ; Morphogenesis/genetics ; Phenotype ; Wnt Signaling Pathway ; Wnt1 Protein/chemistry ; Wnt1 Protein/genetics ; Wnt1 Protein/metabolism
    Chemical Substances Biomarkers ; Drosophila Proteins ; Wnt1 Protein ; wg protein, Drosophila
    Language English
    Publishing date 2018-04-05
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 2167-2
    ISSN 1943-2631 ; 0016-6731
    ISSN (online) 1943-2631
    ISSN 0016-6731
    DOI 10.1534/genetics.117.300157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Wingless/Wnt signaling in Drosophila: the pattern and the pathway.

    Bejsovec, Amy

    Molecular reproduction and development

    2013  Volume 80, Issue 11, Page(s) 882–894

    Abstract: Wnt signaling generates pattern in all animal embryos, from flies and worms to humans, and promotes the undifferentiated, proliferative state critical for stem cells in adult tissues. Inappropriate Wnt pathway activation is the major cause of colorectal ... ...

    Abstract Wnt signaling generates pattern in all animal embryos, from flies and worms to humans, and promotes the undifferentiated, proliferative state critical for stem cells in adult tissues. Inappropriate Wnt pathway activation is the major cause of colorectal cancers, a leading cause of cancer death in humans. Although this pathway has been studied extensively for years, large gaps remain in our understanding of how it switches on and off, and how its activation changes cellular behaviors. Much of what is known about the pathway comes from genetic studies in Drosophila, where a single Wnt molecule, encoded by wingless (wg), directs an array of cell-fate decisions similar to those made by the combined activities of all 19 Wnt family members in vertebrates. Although Wg specifies fate in many tissues, including the brain, limbs, and major organs, the fly embryonic epidermis has proven to be a very powerful system for dissecting pathway activity. It is a simple, accessible tissue, with a pattern that is highly sensitive to small changes in Wg pathway activity. This review discusses what we have learned about Wnt signaling from studying mutations that disrupt epidermal pattern in the fly embryo, highlights recent advances and controversies in the field, and sets these issues in the context of questions that remain about how this essential signaling pathway functions.
    MeSH term(s) Animals ; Drosophila/embryology ; Drosophila/growth & development ; Drosophila/physiology ; Mutation ; Signal Transduction ; Wnt Proteins
    Chemical Substances Wnt Proteins
    Language English
    Publishing date 2013-09-18
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Review
    ZDB-ID 20321-x
    ISSN 1098-2795 ; 1040-452X
    ISSN (online) 1098-2795
    ISSN 1040-452X
    DOI 10.1002/mrd.22228
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: SoxNeuro and Shavenbaby act cooperatively to shape denticles in the embryonic epidermis of

    Rizzo, Nicholas P / Bejsovec, Amy

    Development (Cambridge, England)

    2017  Volume 144, Issue 12, Page(s) 2248–2258

    Abstract: During development, extracellular signals are integrated by cells to induce the transcriptional circuitry that controls morphogenesis. In the fly epidermis, Wingless (Wg)/Wnt signaling directs cells to produce either a distinctly shaped denticle or no ... ...

    Abstract During development, extracellular signals are integrated by cells to induce the transcriptional circuitry that controls morphogenesis. In the fly epidermis, Wingless (Wg)/Wnt signaling directs cells to produce either a distinctly shaped denticle or no denticle, resulting in a segmental pattern of denticle belts separated by smooth, or 'naked', cuticle. Naked cuticle results from Wg repression of
    MeSH term(s) Animals ; Animals, Genetically Modified ; Binding Sites ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/metabolism ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/embryology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Epidermis/embryology ; Epidermis/metabolism ; Gene Expression Regulation, Developmental ; Genes, Insect ; Models, Biological ; Morphogenesis/genetics ; Morphogenesis/physiology ; Mutation ; Promoter Regions, Genetic ; SOX Transcription Factors/genetics ; SOX Transcription Factors/metabolism ; Signal Transduction ; Transcription Factors/genetics ; Transcription Factors/metabolism ; Wnt1 Protein/genetics ; Wnt1 Protein/metabolism
    Chemical Substances DNA-Binding Proteins ; Drosophila Proteins ; SOX Transcription Factors ; SoxN protein, Drosophila ; Transcription Factors ; Wnt1 Protein ; ovo protein, Drosophila ; wg protein, Drosophila
    Language English
    Publishing date 2017-05-15
    Publishing country England
    Document type Journal Article
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.150169
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Flying at the head of the pack: Wnt biology in Drosophila.

    Bejsovec, A

    Oncogene

    2006  Volume 25, Issue 57, Page(s) 7442–7449

    Abstract: The fruitfly, Drosophila melanogaster, has been of central importance in analysing the mechanics of cellular processes. Classic forward genetic screens in the fly have identified many of the genes that define critical cell signaling pathways, for example. ...

    Abstract The fruitfly, Drosophila melanogaster, has been of central importance in analysing the mechanics of cellular processes. Classic forward genetic screens in the fly have identified many of the genes that define critical cell signaling pathways, for example. Our understanding of the Wnt pathway, in particular, has benefited from the many advantages that the fly offers as a model system. Here, I review the history of these discoveries and highlight the utility of the fly in dissecting the molecular workings of Wnt signal transduction.
    MeSH term(s) Animals ; Body Patterning ; Drosophila melanogaster/metabolism ; Drosophila melanogaster/physiology ; Models, Biological ; Models, Genetic ; Mutation ; Phenotype ; Signal Transduction ; Wnt Proteins/metabolism
    Chemical Substances Wnt Proteins
    Language English
    Publishing date 2006-12-04
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Review
    ZDB-ID 639046-8
    ISSN 1476-5594 ; 0950-9232
    ISSN (online) 1476-5594
    ISSN 0950-9232
    DOI 10.1038/sj.onc.1210051
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Wnt pathway activation: new relations and locations.

    Bejsovec, Amy

    Cell

    2005  Volume 120, Issue 1, Page(s) 11–14

    Abstract: Recent advances in the Wnt signaling field reveal new components, such as a G protein and an atypical receptor tyrosine kinase, and novel connections between known components. In addition, different subcellular localization of receptors may help to ... ...

    Abstract Recent advances in the Wnt signaling field reveal new components, such as a G protein and an atypical receptor tyrosine kinase, and novel connections between known components. In addition, different subcellular localization of receptors may help to explain distinctions between canonical and noncanonical Wnt pathway activity.
    MeSH term(s) Animals ; GTP-Binding Proteins/metabolism ; Intercellular Signaling Peptides and Proteins/metabolism ; Models, Biological ; Protein-Tyrosine Kinases/metabolism ; Signal Transduction/physiology ; Wnt Proteins
    Chemical Substances Intercellular Signaling Peptides and Proteins ; Wnt Proteins ; Protein-Tyrosine Kinases (EC 2.7.10.1) ; GTP-Binding Proteins (EC 3.6.1.-)
    Language English
    Publishing date 2005-01-14
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 187009-9
    ISSN 1097-4172 ; 0092-8674
    ISSN (online) 1097-4172
    ISSN 0092-8674
    DOI 10.1016/j.cell.2004.12.021
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: crinkled reveals a new role for Wingless signaling in Drosophila denticle formation.

    Bejsovec, Amy / Chao, Anna T

    Development (Cambridge, England)

    2012  Volume 139, Issue 4, Page(s) 690–698

    Abstract: The specification of the body plan in vertebrates and invertebrates is controlled by a variety of cell signaling pathways, but how signaling output is translated into morphogenesis is an ongoing question. Here, we describe genetic interactions between ... ...

    Abstract The specification of the body plan in vertebrates and invertebrates is controlled by a variety of cell signaling pathways, but how signaling output is translated into morphogenesis is an ongoing question. Here, we describe genetic interactions between the Wingless (Wg) signaling pathway and a nonmuscle myosin heavy chain, encoded by the crinkled (ck) locus in Drosophila. In a screen for mutations that modify wg loss-of-function phenotypes, we isolated multiple independent alleles of ck. These ck mutations dramatically alter the morphology of the hook-shaped denticles that decorate the ventral surface of the wg mutant larval cuticle. In an otherwise wild-type background, ck mutations do not significantly alter denticle morphology, suggesting a specific interaction with Wg-mediated aspects of epidermal patterning. Here, we show that changing the level of Wg activity changes the structure of actin bundles during denticle formation in ck mutants. We further find that regulation of the Wg target gene, shaven-baby (svb), and of its transcriptional targets, miniature (m) and forked (f), modulates this ck-dependent process. We conclude that Ck acts in concert with Wg targets to orchestrate the proper shaping of denticles in the Drosophila embryonic epidermis.
    MeSH term(s) Actins/metabolism ; Animals ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/anatomy & histology ; Drosophila melanogaster/embryology ; Drosophila melanogaster/physiology ; Embryo, Nonmammalian/anatomy & histology ; Embryo, Nonmammalian/physiology ; Gene Expression Regulation, Developmental ; Humans ; Morphogenesis/physiology ; Phenotype ; Signal Transduction/physiology ; Wnt1 Protein/genetics ; Wnt1 Protein/metabolism
    Chemical Substances Actins ; Drosophila Proteins ; Wnt1 Protein ; ck protein, Drosophila ; wg protein, Drosophila
    Language English
    Publishing date 2012-01-04
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.074013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Wnt signaling: an embarrassment of receptors.

    Bejsovec, A

    Current biology : CB

    2000  Volume 10, Issue 24, Page(s) R919–22

    Abstract: Recent genetic studies in Drosophila and mouse have uncovered a new aspect of the Wnt signal transduction machinery. Mutations disrupting LDL-receptor related proteins produce loss-of-function Wnt phenotypes, suggesting that these cell surface molecules ... ...

    Abstract Recent genetic studies in Drosophila and mouse have uncovered a new aspect of the Wnt signal transduction machinery. Mutations disrupting LDL-receptor related proteins produce loss-of-function Wnt phenotypes, suggesting that these cell surface molecules may represent essential co-receptors for Wnt ligands.
    MeSH term(s) Animals ; Drosophila Proteins ; Frizzled Receptors ; Membrane Glycoproteins/metabolism ; Membrane Proteins/metabolism ; Mitogens/genetics ; Mitogens/metabolism ; Models, Biological ; Morphogenesis/genetics ; Proteoglycans/metabolism ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/metabolism ; Receptors, G-Protein-Coupled ; Receptors, LDL/genetics ; Receptors, LDL/metabolism ; Signal Transduction/physiology ; Wnt Proteins ; Zebrafish Proteins
    Chemical Substances Drosophila Proteins ; Frizzled Receptors ; Membrane Glycoproteins ; Membrane Proteins ; Mitogens ; Proteoglycans ; Proto-Oncogene Proteins ; Receptors, G-Protein-Coupled ; Receptors, LDL ; Wnt Proteins ; Zebrafish Proteins ; dally protein, Drosophila ; fz protein, Drosophila
    Language English
    Publishing date 2000-12
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Review
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/s0960-9822(00)00852-6
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article: Wnt signalling shows its versatility.

    Bejsovec, A

    Current biology : CB

    1999  Volume 9, Issue 18, Page(s) R684–7

    Abstract: Wnt signalling controls many different cell fate choices in a wide variety of animal species. Recent studies have revealed that regulatory interactions at several steps in the pathway can modify its outcome, helping to explain how the same pathway can, ... ...

    Abstract Wnt signalling controls many different cell fate choices in a wide variety of animal species. Recent studies have revealed that regulatory interactions at several steps in the pathway can modify its outcome, helping to explain how the same pathway can, in different contexts, have very different characteristics and consequences.
    MeSH term(s) Animals ; Armadillo Domain Proteins ; Caenorhabditis elegans/embryology ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/physiology ; Caenorhabditis elegans Proteins ; Calcium-Calmodulin-Dependent Protein Kinases/genetics ; Calcium-Calmodulin-Dependent Protein Kinases/physiology ; DNA-Binding Proteins/genetics ; DNA-Binding Proteins/physiology ; Drosophila Proteins ; Drosophila melanogaster/embryology ; Drosophila melanogaster/genetics ; Drosophila melanogaster/physiology ; Gene Expression Regulation, Developmental ; Glycogen Synthase Kinase 3 ; Helminth Proteins/genetics ; Helminth Proteins/physiology ; High Mobility Group Proteins/genetics ; High Mobility Group Proteins/physiology ; Insect Proteins/genetics ; Insect Proteins/physiology ; Morphogenesis/genetics ; Proto-Oncogene Proteins/genetics ; Proto-Oncogene Proteins/physiology ; Signal Transduction/physiology ; Trans-Activators ; Transcription Factors/genetics ; Transcription Factors/physiology ; Wnt Proteins ; Wnt1 Protein ; Zebrafish Proteins
    Chemical Substances ARM protein, Drosophila ; Armadillo Domain Proteins ; Caenorhabditis elegans Proteins ; DNA-Binding Proteins ; Drosophila Proteins ; Helminth Proteins ; High Mobility Group Proteins ; Insect Proteins ; Proto-Oncogene Proteins ; Trans-Activators ; Transcription Factors ; Wnt Proteins ; Wnt1 Protein ; Zebrafish Proteins ; ovo protein, Drosophila ; pop-1 protein, C elegans ; wg protein, Drosophila ; Calcium-Calmodulin-Dependent Protein Kinases (EC 2.7.11.17) ; Glycogen Synthase Kinase 3 (EC 2.7.11.26)
    Language English
    Publishing date 1999-09-23
    Publishing country England
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Review
    ZDB-ID 1071731-6
    ISSN 1879-0445 ; 0960-9822
    ISSN (online) 1879-0445
    ISSN 0960-9822
    DOI 10.1016/s0960-9822(99)80439-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Characterization of

    Cotsworth, Shawn / Jackson, Catherine J / Hallson, Graham / Fitzpatrick, Kathleen A / Syrzycka, Monika / Coulthard, Alistair B / Bejsovec, Amy / Marchetti, Marcella / Pimpinelli, Sergio / Wang, Simon J H / Camfield, Robert G / Verheyen, Esther M / Sinclair, Donald A / Honda, Barry M / Hilliker, Arthur J

    Cells

    2022  Volume 11, Issue 3

    Abstract: ... ...

    Abstract The
    MeSH term(s) Animals ; Biosynthetic Pathways/genetics ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Euchromatin ; Glutamine/metabolism ; Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/genetics ; Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing)/metabolism ; Hexosamines
    Chemical Substances Euchromatin ; Hexosamines ; Glutamine (0RH81L854J) ; Glutamine-Fructose-6-Phosphate Transaminase (Isomerizing) (EC 2.6.1.16)
    Language English
    Publishing date 2022-01-27
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells11030448
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Pebble/ECT2 RhoGEF negatively regulates the Wingless/Wnt signaling pathway.

    Greer, Elisabeth R / Chao, Anna T / Bejsovec, Amy

    Development (Cambridge, England)

    2013  Volume 140, Issue 24, Page(s) 4937–4946

    Abstract: Wingless (Wg)/Wnt signaling is essential for patterning invertebrate and vertebrate embryos, and inappropriate Wnt activity is associated with a variety of human cancers. Despite intensive study, Wnt pathway mechanisms are not fully understood. We have ... ...

    Abstract Wingless (Wg)/Wnt signaling is essential for patterning invertebrate and vertebrate embryos, and inappropriate Wnt activity is associated with a variety of human cancers. Despite intensive study, Wnt pathway mechanisms are not fully understood. We have discovered a new mechanism for regulating the Wnt pathway: activity of a Rho guanine nucleotide exchange factor (GEF) encoded by pebble (pbl) in Drosophila and ECT2 in humans. This RhoGEF has an essential role in cytokinesis, but also plays an unexpected, conserved role in inhibiting Wg/Wnt activity. Loss and gain of pbl function in Drosophila embryos cause pattern defects that indicate altered Wg activity. Both Pbl and ECT2 repress Wg/Wnt target gene expression in cultured Drosophila and human cells. The GEF activity is required for Wnt regulation, whereas other protein domains important for cytokinesis are not. Unlike most negative regulators of Wnt activity, Pbl/ECT2 functions downstream of Armadillo (Arm)/beta-catenin stabilization. Our results indicate GTPase regulation at a novel point in Wg/Wnt signal transduction, and provide new insight into the categorization of ECT2 as a human proto-oncogene.
    MeSH term(s) Animals ; Armadillo Domain Proteins/genetics ; Body Patterning/genetics ; Cell Line ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/metabolism ; GTP Phosphohydrolases/metabolism ; Gene Expression Regulation, Developmental ; Guanine Nucleotide Exchange Factors/genetics ; Guanine Nucleotide Exchange Factors/metabolism ; HEK293 Cells ; Humans ; Proto-Oncogene Proteins/metabolism ; RNA Interference ; RNA, Small Interfering ; Transcription Factors/genetics ; Wnt Signaling Pathway ; Wnt1 Protein/metabolism ; beta Catenin/genetics ; rho GTP-Binding Proteins/metabolism
    Chemical Substances ARM protein, Drosophila ; Armadillo Domain Proteins ; Drosophila Proteins ; ECT2 protein, human ; Guanine Nucleotide Exchange Factors ; Pbl protein, Drosophila ; Proto-Oncogene Proteins ; RNA, Small Interfering ; Transcription Factors ; Wnt1 Protein ; beta Catenin ; wg protein, Drosophila ; GTP Phosphohydrolases (EC 3.6.1.-) ; Rho1 protein, Drosophila (EC 3.6.5.2) ; rho GTP-Binding Proteins (EC 3.6.5.2)
    Language English
    Publishing date 2013-11-06
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 90607-4
    ISSN 1477-9129 ; 0950-1991
    ISSN (online) 1477-9129
    ISSN 0950-1991
    DOI 10.1242/dev.101303
    Database MEDical Literature Analysis and Retrieval System OnLINE

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