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Article ; Online: Corrigendum to "Novel LRR-ROC Motif That Links the N- and C-terminal Domains in LRRK2 Undergoes an Order-Disorder Transition Upon Activation" [J. Mol. Biol. 435(12) (2023) 1-17].

Weng, Jui-Hung / Trilling, Chiara R / Kaila Sharma, Pallavi / Störmer, Eliza / Wu, Jian / Herberg, Friedrich W / Taylor, Susan S

Journal of molecular biology

2024 Volume 436, Issue 6, Page(s) 168485

Language	English
Publishing date	2024-02-17
Publishing country	Netherlands
Document type	Published Erratum
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2024.168485
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Zs.A 867: Show issues

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Article ; Online: Reply to M.C. Chamberlain and T.J. Kruser.

Verschraegen, Claire F / Thomas, Alissa / Vinh-Hung, Vincent

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

2016 Volume 34, Issue 15, Page(s) 1827–1828

MeSH term(s)	Cranial Irradiation ; Glioblastoma ; Humans
Language	English
Publishing date	2016--20
Publishing country	United States
Document type	Letter ; Comment
ZDB-ID	604914-x
ISSN	1527-7755 ; 0732-183X
ISSN (online)	1527-7755
ISSN	0732-183X
DOI	10.1200/JCO.2016.66.6248
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Zs.A 1847: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MO 650: Show issues

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Article ; Online: Apolipoprotein J, a glucose-upregulated molecular chaperone, stabilizes core and NS5A to promote infectious hepatitis C virus virion production.

Lin, Chun-Chieh / Tsai, Peiju / Sun, Hung-Yu / Hsu, Mei-Chi / Lee, Jin-Ching / Wu, I-Chin / Tsao, Chiung-Wen / Chang, Ting-Tsung / Young, Kung-Chia

Journal of hepatology

2014 Volume 61, Issue 5, Page(s) 984–993

Abstract: Background & aims: Hepatitis C virus (HCV) infection leads to glucose abnormality. HCV depends ... and locations for this process remain unidentified. Apolipoprotein J (ApoJ), upregulated by glucose ... primary human hepatocytes, and in treatment naive chronic hepatitis C patients. How ApoJ affects HCV ...

Abstract	Background & aims: Hepatitis C virus (HCV) infection leads to glucose abnormality. HCV depends on lipid droplets (LDs) and very-low density lipoproteins for assembly/releasing; however, the components and locations for this process remain unidentified. Apolipoprotein J (ApoJ), upregulated by glucose, functions as Golgi chaperone of secreted proteins and resides abundantly in very-low density lipoproteins. This study investigates the interplay between glucose, ApoJ and HCV virion production. Methods: The effects of high glucose on ApoJ expression and HCV production were evaluated with cultivated HuH7.5, primary human hepatocytes, and in treatment naive chronic hepatitis C patients. How ApoJ affects HCV lifecycle was assessed using siRNA knockdown strategy in JFH1 infected and subgenomic replicon cells. The interactions and locations of ApoJ with viral and host components were examined by immunoprecipitation, immunofluorescence and subcellular fractionation experiments. Results: HCV infection increased ApoJ expression, which in parallel with HCV infectivity was additionally elevated with high glucose treatment. Serum ApoJ correlated positively with fasting blood glucose concentration and HCV-RNA titre in patients. ApoJ silencing reduced intracellular and extracellular HCV infectivity and extracellular HCV-RNA, but accumulated intracellular HCV-RNA in HCV-infected cells. ApoJ interacted with HCV core and NS5A and stabilized the dual protein complex. HCV infection dispersed cytoplasmic ApoJ from the compact zones of the Golgi to encircle LDs, where co-localization of the core, NS5A, HCV-RNA, subcellular markers for LDs, endoplasmic reticulum (ER), Golgi, and membrane contact sites occurred. Conclusions: ApoJ facilitates infectious HCV particle production via stabilization of core/NS5A, which might surround LDs at the ER-Golgi membrane contact site.
MeSH term(s)	Adult ; Aged ; Cell Line ; Clusterin/metabolism ; Diabetes Mellitus, Type 2/complications ; Female ; Glucose/metabolism ; Hepacivirus/pathogenicity ; Hepacivirus/physiology ; Hepatitis C, Chronic/complications ; Hepatitis C, Chronic/metabolism ; Hepatitis C, Chronic/virology ; Hepatocytes/metabolism ; Hepatocytes/virology ; Humans ; Male ; Middle Aged ; Models, Biological ; Protein Stability ; Up-Regulation ; Viral Core Proteins/metabolism ; Viral Nonstructural Proteins/metabolism ; Virion/pathogenicity ; Virion/physiology ; Virus Replication
Chemical Substances	CLU protein, human ; Clusterin ; Viral Core Proteins ; Viral Nonstructural Proteins ; NS-5 protein, hepatitis C virus (EC 2.7.7.48) ; Glucose (IY9XDZ35W2)
Language	English
Publishing date	2014-07-01
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	605953-3
ISSN	1600-0641 ; 0168-8278
ISSN (online)	1600-0641
ISSN	0168-8278
DOI	10.1016/j.jhep.2014.06.026
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Zs.A 2027: Show issues

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Article: Impaired V(D)J recombination and increased apoptosis among B cell precursors in the bone marrow of c-Abl-deficient mice.

Lam, Queenie Lai Kwan / Lo, Cherry Kam Chun / Zheng, Bo-Jian / Ko, King-Hung / Osmond, Dennis G / Wu, Gillian E / Rottapel, Robert / Lu, Liwei

International immunology

2007 Volume 19, Issue 3, Page(s) 267–276

Abstract: Previous studies on c-Abl-deficient mice have shown high post-natal mortality and lymphopenia ... However, the mechanisms by which c-Abl may influence B lymphopoiesis remain obscure. In this study, we analyzed B cell sub ... populations at various differentiation stages in the bone marrow (BM) of c-Abl-deficient mice. Phenotypic ...

Abstract	Previous studies on c-Abl-deficient mice have shown high post-natal mortality and lymphopenia. However, the mechanisms by which c-Abl may influence B lymphopoiesis remain obscure. In this study, we analyzed B cell sub-populations at various differentiation stages in the bone marrow (BM) of c-Abl-deficient mice. Phenotypic analyses revealed that c-Abl(-/-) pro-B cells were reduced to half of normal incidence and absolute number, while pre-B cells showed an even greater reduction. Both c-Abl(-/-) pro-B and pre-B cell populations showed considerably elevated apoptosis ex vivo and in short-term culture but their cell cycle progression was not impaired. In contrast, apoptosis of immature IgM(+)IgD(-) B lymphocytes remained at normal control levels. Inhibition of c-Abl activity by STI571 in normal BM cultures significantly increased apoptosis in B cell precursors while the survival of immature B cells was not affected. To determine whether c-Abl deficiency affects Ig heavy-chain rearrangement, we found that the frequency of V(D)J recombination was markedly reduced by 15-fold in c-Abl(-/-) pro-B cells compared with the control values. However, no perturbation in the levels of signal-end recombination intermediates was found. Taken together, we propose that c-Abl mediates a stage-specific anti-apoptotic response in precursor B cells and is required for efficient V(D)J recombination during B cell development.
MeSH term(s)	Animals ; Apoptosis/genetics ; B-Lymphocytes/cytology ; B-Lymphocytes/metabolism ; Bone Marrow Cells/cytology ; Bone Marrow Cells/metabolism ; Cell Cycle/genetics ; Cell Differentiation/genetics ; Cell Lineage/genetics ; Cells, Cultured ; Gene Rearrangement, B-Lymphocyte ; Immunoglobulin Heavy Chains/genetics ; Lymphopoiesis/genetics ; Mice ; Mice, Knockout ; Phenotype ; Proto-Oncogene Proteins c-abl/deficiency ; Proto-Oncogene Proteins c-abl/genetics ; Recombination, Genetic ; VDJ Recombinases/metabolism
Chemical Substances	Immunoglobulin Heavy Chains ; Proto-Oncogene Proteins c-abl (EC 2.7.10.2) ; VDJ Recombinases (EC 2.7.7.-)
Language	English
Publishing date	2007-03
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1013745-2
ISSN	1460-2377 ; 0953-8178
ISSN (online)	1460-2377
ISSN	0953-8178
DOI	10.1093/intimm/dxl143
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Zs.A 2619: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 70: Show issues

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Article ; Online: Response to: Risk of hemorrhagic stroke after venomous snakebite: correspondence.

Hung, W-H / Hung, Y-M / Yip, H-T / Wei, J C-C / Chang, R

QJM : monthly journal of the Association of Physicians

2022 Volume 116, Issue 4, Page(s) 324–325

MeSH term(s)	Humans ; Snake Bites/complications ; Venoms ; Hemorrhagic Stroke ; Antivenins/therapeutic use
Chemical Substances	Venoms ; Antivenins
Language	English
Publishing date	2022-05-31
Publishing country	England
Document type	Journal Article ; Comment
ZDB-ID	1199985-8
ISSN	1460-2393 ; 0033-5622 ; 1460-2725
ISSN (online)	1460-2393
ISSN	0033-5622 ; 1460-2725
DOI	10.1093/qjmed/hcac131
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Article ; Online: A repository of COVID-19 related molecular dynamics simulations and utilisation in the context of nsp10-nsp16 antivirals.

Liang, Julia J / Pitsillou, Eleni / Hung, Andrew / Karagiannis, Tom C

Journal of molecular graphics & modelling

2023 Volume 126, Page(s) 108666

Abstract: The coronavirus disease 2019 (COVID-19) pandemic highlighted the importance of establishing systems and infrastructure to develop vaccines, antiviral drugs, and therapeutic antibodies against emerging pathogens. Typical drug discovery processes involve ... ...

Abstract	The coronavirus disease 2019 (COVID-19) pandemic highlighted the importance of establishing systems and infrastructure to develop vaccines, antiviral drugs, and therapeutic antibodies against emerging pathogens. Typical drug discovery processes involve targeting suitable proteins to effect pathogen replication or to attenuate host responses, by examining either large chemical databases or protein-protein interactions. Following initial screens, molecular dynamics (MD) simulations are critical for gaining further insight into molecular interactions. During the COVID-19 pandemic, many research groups made their simulations widely available, as highlighted by the comprehensive D.E. Shaw Research trajectory database. To investigate protein target sites and evaluate potential lead compounds, we performed over 300 MD simulations relating to COVID-19. We organised our simulations into a repository, which is publicly available at https://epimedlab.org/trajectories/. The trajectories cover a large part of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteome, and the majority of our MD simulations focused on the identification of potential antivirals. For example, we focused on the S-adenosyl-l-methionine binding site of the nsp10-nsp16 complex, a critical component of viral replication, revealing verbascoside as a potential lead. Moreover, we utilised MD trajectories to explore the interface between the spike protein receptor binding domain and human angiotensin-converting enzyme 2 receptor, with the ultimate aim being investigation of new variants in real-time. Overall, MD simulations are a critical component of the in silico drug discovery process and as highlighted throughout the pandemic, data sharing enables accelerated progress. We have organised our extensive collection of COVID-19 related MD trajectories into an easily accessible repository.
MeSH term(s)	Humans ; COVID-19 ; Molecular Dynamics Simulation ; SARS-CoV-2 ; Pandemics ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Antiviral Agents/chemistry
Chemical Substances	Antiviral Agents
Language	English
Publishing date	2023-11-10
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1396450-1
ISSN	1873-4243 ; 1093-3263
ISSN (online)	1873-4243
ISSN	1093-3263
DOI	10.1016/j.jmgm.2023.108666
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Zs.A 3491: Show issues

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Article ; Online: Super-Resolution Imaging of Voltages in the Interior of Individual, Vital Mitochondria.

Lee, ChiaHung / Wallace, Douglas C / Burke, Peter J

ACS nano

2023 Volume 18, Issue 2, Page(s) 1345–1356

Abstract: We present super-resolution microscopy of isolated functional mitochondria, enabling real-time studies of structure and function (voltages) in response to pharmacological manipulation. Changes in mitochondrial membrane potential as a function of time and ...

Abstract	We present super-resolution microscopy of isolated functional mitochondria, enabling real-time studies of structure and function (voltages) in response to pharmacological manipulation. Changes in mitochondrial membrane potential as a function of time and position can be imaged in different metabolic states (not possible in whole cells), created by the addition of substrates and inhibitors of the electron transport chain, enabled by the isolation of vital mitochondria. By careful analysis of structure dyes and voltage dyes (lipophilic cations), we demonstrate that most of the fluorescent signal seen from voltage dyes is due to membrane bound dyes, and develop a model for the membrane potential dependence of the fluorescence contrast for the case of super-resolution imaging, and how it relates to membrane potential. This permits direct analysis of mitochondrial structure and function (voltage) of isolated, individual mitochondria as well as submitochondrial structures in the functional, intact state, a major advance in super-resolution studies of living organelles.
MeSH term(s)	Mitochondria/metabolism ; Organelles/metabolism ; Microscopy/methods ; Membrane Potentials ; Coloring Agents ; Fluorescent Dyes/chemistry
Chemical Substances	Coloring Agents ; Fluorescent Dyes
Language	English
Publishing date	2023-06-08
Publishing country	United States
Document type	Journal Article
ISSN	1936-086X
ISSN (online)	1936-086X
DOI	10.1021/acsnano.3c02768
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Article ; Online: Ultrasound accuracy for brachial plexus pathology.

Yip, S W Y / Griffith, J F / Tong, C S L / Cheung, K K / Tsoi, C / Hung, E H Y

Clinical radiology

2024

Abstract: Aim: To determine (a) the accuracy of ultrasound in detecting brachial plexus pathology and (b) outline the advantages and limitations of ultrasound compared to MRI for imaging the brachial plexus.: Material and methods: cases with clinically ... ...

Abstract	Aim: To determine (a) the accuracy of ultrasound in detecting brachial plexus pathology and (b) outline the advantages and limitations of ultrasound compared to MRI for imaging the brachial plexus. Material and methods: cases with clinically suspected brachial plexus pathology were evaluated first by ultrasound, followed by MRI. Patients with prior brachial plexus imaging were excluded. The final diagnosis was based on a combination of ultrasound, MRI, clinical follow-up, and surgical findings. The accuracy of the ultrasound was assessed by comparing the ultrasound and the final diagnoses. The mean clinical follow-up time following ultrasound was 1.8 ± 1.4 years. Results: Ninety-two (64%) of the 143 cases had normal brachial plexus ultrasound and MRI examinations. Fifty-one (36%) of 143 cases had brachial plexus pathology on MRI, comprising post-radiation fibrosis (n=25, 49%), nerve sheath tumor (n=11, 21%), traumatic injury (n=7, 14%), inflammatory polyneuropathy (n=4, 8%), malignant infiltration (n=2, 4%), desmoid fibromatosis (n=1,2%), and neuralgic amyotrophy (n=1, 2%). Overall diagnostic accuracy of ultrasound for brachial plexus pathology was 98% (140/143), with three discordant cases (neuralgic amyotrophy n=1, inflammatory neuropathy n=1, postradiation fibrosis n=1) regarded as normal on ultrasound assessment. Sensitivity, specificity, and positive and negative predictive value of ultrasound for identifying brachial plexus pathology were 94%, 100%, 100%, and 97%, respectively. Conclusion: Ultrasound identifies brachial plexus pathology with high accuracy and specificity, showing comparable diagnostic efficacy to MRI. Ultrasound can serve as an effective first-line imaging investigation for suspected brachial plexus pathology.
Language	English
Publishing date	2024-03-22
Publishing country	England
Document type	Journal Article
ZDB-ID	391227-9
ISSN	1365-229X ; 0009-9260
ISSN (online)	1365-229X
ISSN	0009-9260
DOI	10.1016/j.crad.2024.03.010
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Ue I Zs.201: Show issues

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Article ; Online: Effectiveness of mouth rinses against COVID-19: a systematic review and network meta-analysis.

Lin, S-Y / Sun, J-S / Hung, M-C / Chang, J Z-C

The Journal of hospital infection

2023 Volume 139, Page(s) 175–191

Abstract: Objective: This systematic review and network meta-analysis (NMA) comprehensively compared the effectiveness of different mouth rinses in reducing the viral load/infectivity of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (Part I), ... ...

Abstract	Objective: This systematic review and network meta-analysis (NMA) comprehensively compared the effectiveness of different mouth rinses in reducing the viral load/infectivity of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) (Part I), alleviating clinical symptoms or severity of disease (Part II), and decreasing the incidence of SARS-CoV-2 infection (Part III). Methods: Randomized controlled trials (RCTs) and non-randomized controlled trials (NRCTs) with restrictions were searched up to 3 Results: Five RCTs (454 patients and nine interventions) in Part I were eligible for NMA. The NMA results showed that, in comparison with no rinse, sodium chloride (NaCl) was the most effective mouth rinse for reducing the viral load, followed by povidone-iodine (PVP-I), ß-cyclodextrin + citrox (CDCM), hydrogen peroxide (HP), chlorhexidine gluconate (CHX), cetylpyridinium chloride (CPC), placebo and hypochlorous acid (HClO). However, these results were not significant. Based on surface under the cumulative ranking curve scores, PVP-I was likely to be the most efficacious mouth rinse for reducing SARS-CoV-2 viral load, followed by CDCM, HP, NaCl, CHX, CPC, placebo, no rinse and HClO. Conclusion: Due to heterogeneity of the primary studies, the effectiveness of different mouth rinses to reduce viral infectivity, improve clinical symptoms or prevent SARS-CoV-2 infection remains inconclusive.
MeSH term(s)	Humans ; COVID-19 ; Mouthwashes/therapeutic use ; Povidone-Iodine ; SARS-CoV-2 ; Sodium Chloride/therapeutic use ; Network Meta-Analysis ; Hydrogen Peroxide ; Mouth
Chemical Substances	Mouthwashes ; Povidone-Iodine (85H0HZU99M) ; Sodium Chloride (451W47IQ8X) ; Hydrogen Peroxide (BBX060AN9V)
Language	English
Publishing date	2023-07-06
Publishing country	England
Document type	Meta-Analysis ; Systematic Review ; Journal Article ; Review
ZDB-ID	779366-2
ISSN	1532-2939 ; 0195-6701
ISSN (online)	1532-2939
ISSN	0195-6701
DOI	10.1016/j.jhin.2023.06.022
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Article: Metabolic Engineering Design Strategies for Increasing Carbon Fluxes Relevant for Biosynthesis in Cyanobacteria.

Chen, Arvin Y / Ku, Jason T / Tsai, Teresa P / Hung, Jenny J / Hung, Billy C / Lan, Ethan I

Advances in biochemical engineering/biotechnology

2023 Volume 183, Page(s) 105–144

Abstract: Cyanobacteria are promising microbial cell factories for the direct production of biochemicals and biofuels from ... ...

Abstract	Cyanobacteria are promising microbial cell factories for the direct production of biochemicals and biofuels from CO
MeSH term(s)	Metabolic Engineering ; Cyanobacteria/genetics ; Cyanobacteria/metabolism ; Photosynthesis/genetics ; Pyruvic Acid/metabolism ; Carbon/metabolism
Chemical Substances	Pyruvic Acid (8558G7RUTR) ; Carbon (7440-44-0)
Language	English
Publishing date	2023-04-11
Publishing country	Germany
Document type	Journal Article
ISSN	0724-6145
ISSN	0724-6145
DOI	10.1007/10_2023_218
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