Article ; Online: Co-Immunoprecipitation (Co-Ip) in Mammalian Cells.
Methods in molecular biology (Clifton, N.J.)
2023 Volume 2655, Page(s) 67–77
Abstract: The cell is a fantastic place where molecules dynamically move through the various cellular structures and compartments and meet each other, either transiently or in more stable complexes. These complexes have always a specific biological function; thus, ...
| Abstract | The cell is a fantastic place where molecules dynamically move through the various cellular structures and compartments and meet each other, either transiently or in more stable complexes. These complexes have always a specific biological function; thus, it is important to identify and characterize the interaction between molecules, either DNA/RNA, DNA/DNA, protein/DNA, protein/protein, and so on. polycomb group proteins (PcG proteins) are epigenetic repressors involved in important physiologic processes as development and differentiation. They act on the chromatin through the formation of a repressive environment involving histone modification, recruitment of co-repressors, and chromatin-chromatin interactions. PcG form multiprotein complexes, whose characterization required several approaches. In this chapter, I will describe the co-immunoprecipitation (Co-IP) protocol, an easy method used to identify and analyze multiprotein complexes. In Co-IP, an antibody is used to isolate its target antigen, along with its binding partners, from a mixed sample. The binding partners purified with the immunoprecipitated protein can be identified by Western blot or by mass spectrometry. |
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| MeSH term(s) | Animals ; Protein Binding ; Polycomb-Group Proteins/genetics ; Chromatin/genetics ; DNA/metabolism ; Immunoprecipitation ; Multiprotein Complexes/metabolism ; Mammals/genetics |
| Chemical Substances | Polycomb-Group Proteins ; Chromatin ; DNA (9007-49-2) ; Multiprotein Complexes |
| Language | English |
| Publishing date | 2023-05-22 |
| Publishing country | United States |
| Document type | Journal Article |
| ISSN | 1940-6029 |
| ISSN (online) | 1940-6029 |
| DOI | 10.1007/978-1-0716-3143-0_6 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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