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Article: Preliminary Findings of the Efficacy of Botulinum Toxin in Temporomandibular Disorders: Uncontrolled Pilot Study.

Blanco-Rueda, José A / López-Valverde, Antonio / Márquez-Vera, Antonio / Méndez-Sánchez, Roberto / López-García, Eva / López-Valverde, Nansi

Life (Basel, Switzerland)

2023 Volume 13, Issue 2

Abstract: Temporomandibular disorders are a common pathology affecting up to 70% of the population, with a maximum incidence in young patients. We used a sample of twenty patients recruited in the Maxillofacial Surgery Service of the University Hospital of ... ...

Abstract	Temporomandibular disorders are a common pathology affecting up to 70% of the population, with a maximum incidence in young patients. We used a sample of twenty patients recruited in the Maxillofacial Surgery Service of the University Hospital of Salamanca (Spain), who met the inclusion criteria, with unilateral painful symptomatology of more than three months' duration. All patients were randomly treated by intramuscular and intra-articular injections of botulinum toxin (100 U) in eight predetermined points. Pain symptomatology was assessed by the visual analog scale (VAS) at the different locations, together with joint symptomatology, at baseline and six weeks after treatment. Adverse effects were also evaluated. In 85% of the patients, pain upon oral opening improved and 90% showed improvement in pain upon mastication. A total of 75% of the patients reported improvement in joint clicking/noise. Headaches improved or disappeared in 70% of the patients treated. Despite the limitations of the study and the preliminary results, intramuscular and intra-articular infiltrations with botulinum toxin were effective in the treatment of symptoms associated with temporomandibular disorders (TMDs), with minimal adverse effects.
Language	English
Publishing date	2023-01-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2662250-6
ISSN	2075-1729
ISSN	2075-1729
DOI	10.3390/life13020345
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Tracing the dissipation of difenoconazole, its metabolites and co-formulants in tomato: A comprehensive analysis by chromatography coupled to high resolution mass spectrometry in laboratory and greenhouse trials.

Maldonado-Reina, Antonio Jesús / López-Ruiz, Rosalía / Marín Sáez, Jesús / Romero-González, Roberto / Garrido Frenich, Antonia

Environmental pollution (Barking, Essex : 1987)

2024 Volume 349, Page(s) 123924

Abstract: The study evaluated Ceremonia 25 EC®, a plant protection product (PPP) containing difenoconazole, in tomato crops, to identify potential risks associated with PPPs, and in addition to this compound, known metabolites from difenoconazole degradation and ... ...

Abstract	The study evaluated Ceremonia 25 EC®, a plant protection product (PPP) containing difenoconazole, in tomato crops, to identify potential risks associated with PPPs, and in addition to this compound, known metabolites from difenoconazole degradation and co-formulants present in the PPP were monitored. An ultra high performance liquid chromatography coupled to quadrupole-Orbitrap mass analyser (UHPLC-Q-Orbitrap-MS) method was validated with a working range of 2 μg/kg (limit of quantification, LOQ) to 200 μg/kg. Difenoconazole degradation followed a biphasic double first-order in parallel (DFOP) kinetic model in laboratory and greenhouse trials, with high accuracy (R
Language	English
Publishing date	2024-04-03
Publishing country	England
Document type	Journal Article
ZDB-ID	280652-6
ISSN	1873-6424 ; 0013-9327 ; 0269-7491
ISSN (online)	1873-6424
ISSN	0013-9327 ; 0269-7491
DOI	10.1016/j.envpol.2024.123924
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Article ; Online: Emotional Competences of Primary Education Teachers: A Need in School Post COVID-19.

Sanz-Ponce, Roberto / López-Luján, Elena / Serrano-Sarmiento, Ángela / Giménez-Beut, Juan Antonio

European journal of investigation in health, psychology and education

2023 Volume 13, Issue 10, Page(s) 1961–1985

Abstract: The COVID-19 pandemic has increased the number of students with mental health problems: depression, anxiety, stress. Faced with this reality, teachers and schools must be prepared to respond quickly and effectively. Therefore, the objective of this ... ...

Abstract	The COVID-19 pandemic has increased the number of students with mental health problems: depression, anxiety, stress. Faced with this reality, teachers and schools must be prepared to respond quickly and effectively. Therefore, the objective of this article is to analyze the emotional competences of primary school teachers in the city of Valencia based on the following sociodemographic variables: sex, age, professional experience, type of center and whether they have children. For this purpose, a quantitative methodological approach has been followed, through which the emotional competencies of primary education teachers are analyzed. These results allow us to establish teacher profiles according to sociodemographic variables and help to detect possible training deficiencies. A sample of 371 teachers of primary education in the city of Valencia has been analyzed. The Questionnaire on Teaching Competences of Primary Education Teachers, carried out under the Planned Action Model, has been used, and descriptive, univariate, bivariate and cluster analyses have been carried out. The mean, the standard deviation and the interquartile range (IQR) have been analyzed, as well as non-parametric tests such as the Wilcoxon, Kruskal-Wallis or Z test. The most significant results are that teachers have a greater ability to interpret emotions and to listen to students. On the contrary, it is teachers who most reject prejudice, discrimination and racism. Younger teachers are the ones who implement more inclusive learning environments. Finally, in general, all teachers are very respectful of students and claim to know how to manage classroom conflicts. The results obtained, in general terms, coincide with most of the research on teachers' emotional competencies. Some aspects simply do not coincide with the literature. The teachers who participated in our research perceive themselves as having a greater capacity to observe and interpret students' emotions, to generate learning situations that cater to diversity and to listen to their students. Other studies place these competencies at lower levels.
Language	English
Publishing date	2023-09-22
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	3041279-1
ISSN	2254-9625 ; 2174-8144
ISSN (online)	2254-9625
ISSN	2174-8144
DOI	10.3390/ejihpe13100141
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Article ; Online: Application of PCR-based diagnostic tools that target Enterocytozoon hepatopenaei for the molecular detection of a Vittaforma-like microsporidium that infects Penaeus vannamei from Costa Rica.

Peña-Navarro, Nelson / López-Carvallo, Antonio / Chacón Perez, Brandon / Cruz-Flores, Roberto

Journal of invertebrate pathology

2023 Volume 200, Page(s) 107958

Abstract: Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the ... ...

Abstract	Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the applicability of two commonly used SSU rRNA methodologies for the detection of additional microsporidia from the genus Vittaforma that is present in cultured Penaeus vannamei from Costa Rica. The molecular detection of DNA of the novel microsporidia can only be achieved using SSU rRNA targeting methodologies and does not cross-react with the highly specific spore wall protein gene PCR detection method.
MeSH term(s)	Animals ; Microsporidia, Unclassified/genetics ; Penaeidae/genetics ; Vittaforma/genetics ; Costa Rica ; Polymerase Chain Reaction/methods ; Enterocytozoon/genetics ; Microsporidia/genetics ; RNA, Ribosomal
Chemical Substances	RNA, Ribosomal
Language	English
Publishing date	2023-07-08
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	390885-9
ISSN	1096-0805 ; 0022-2011
ISSN (online)	1096-0805
ISSN	0022-2011
DOI	10.1016/j.jip.2023.107958
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Article ; Online: Repurposing of rabeprazole as an anti-

García-Torres, Itzhel / De la Mora-De la Mora, Ignacio / López-Velázquez, Gabriel / Cabrera, Nallely / Flores-López, Luis Antonio / Becker, Ingeborg / Herrera-López, Juliana / Hernández, Roberto / Pérez-Montfort, Ruy / Enríquez-Flores, Sergio

Journal of enzyme inhibition and medicinal chemistry

2023 Volume 38, Issue 1, Page(s) 2231169

Abstract: Trypanosoma ... ...

Abstract	Trypanosoma cruzi
MeSH term(s)	Humans ; Trypanosoma cruzi ; Triose-Phosphate Isomerase/chemistry ; Triose-Phosphate Isomerase/pharmacology ; Rabeprazole/pharmacology ; Rabeprazole/therapeutic use ; Drug Repositioning ; Chagas Disease/drug therapy ; Trypanocidal Agents/pharmacology
Chemical Substances	benzonidazole (YC42NRJ1ZD) ; Triose-Phosphate Isomerase (EC 5.3.1.1) ; Rabeprazole (32828355LL) ; Trypanocidal Agents
Language	English
Publishing date	2023-07-03
Publishing country	England
Document type	Journal Article
ZDB-ID	2082578-X
ISSN	1475-6374 ; 1475-6366
ISSN (online)	1475-6374
ISSN	1475-6366
DOI	10.1080/14756366.2023.2231169
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Article ; Online: The emerging pathogen Enterocytozoon hepatopenaei drives a degenerative cyclic pattern in the hepatopancreas microbiome of the shrimp (Penaeus vannamei).

López-Carvallo, Jesús Antonio / Cruz-Flores, Roberto / Dhar, Arun K

Scientific reports

2022 Volume 12, Issue 1, Page(s) 14766

Abstract: The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp (Penaeus spp.) aquaculture worldwide. To prevent diseases in shrimp, the manipulation of the gut microbiota has been suggested. ...

Abstract	The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp (Penaeus spp.) aquaculture worldwide. To prevent diseases in shrimp, the manipulation of the gut microbiota has been suggested. However, prior knowledge of the host-microbiome is necessary. We assessed the modulation of the microbiome (bacteria/fungi) and its predicted functions over the course of disease progression in shrimp experimentally challenged with EHP for 30 days using high throughput 16S rRNA and ITS amplicon sequencing. Infection grade was assessed for the first time by quantitative digital histopathology. According to the infection intensity, three disease-stages (early/developmental/late) were registered. During the early-stage, EHP was not consistently detected, and a high diversity of potentially beneficial microorganisms related to nutrient assimilation were found. In the development-stage, most of the shrimp start to register a high infection intensity related to a decrease in beneficial microorganisms and an increase in opportunistic/pathogenic fungi. During late-stage, animals displayed different infection intensities, showed a displacement of beneficial microorganisms by opportunistic/pathogenic bacteria and fungi related to pathogen infection processes and depletion of energetic reserves. The degenerative cyclic pattern of EHP infection and its effects on beneficial microorganisms and beneficial functions of the shrimp hepatopancreas microbiome are discussed.
MeSH term(s)	Animals ; Enterocytozoon ; Hepatopancreas ; Microbiota ; Penaeidae/microbiology ; Polymerase Chain Reaction ; RNA, Ribosomal, 16S/genetics
Chemical Substances	RNA, Ribosomal, 16S
Language	English
Publishing date	2022-08-30
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-19127-2
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Article ; Online: The emerging pathogen Enterocytozoon hepatopenaei drives a degenerative cyclic pattern in the hepatopancreas microbiome of the shrimp (Penaeus vannamei)

Jesús Antonio López-Carvallo / Roberto Cruz-Flores / Arun K. Dhar

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 15

Abstract: Abstract The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp (Penaeus spp.) aquaculture worldwide. To prevent diseases in shrimp, the manipulation of the gut microbiota has been ... ...

Abstract	Abstract The microsporidian Enterocytozoon hepatopenaei (EHP) is an emerging pathogen that causes substantial economic losses in shrimp (Penaeus spp.) aquaculture worldwide. To prevent diseases in shrimp, the manipulation of the gut microbiota has been suggested. However, prior knowledge of the host-microbiome is necessary. We assessed the modulation of the microbiome (bacteria/fungi) and its predicted functions over the course of disease progression in shrimp experimentally challenged with EHP for 30 days using high throughput 16S rRNA and ITS amplicon sequencing. Infection grade was assessed for the first time by quantitative digital histopathology. According to the infection intensity, three disease-stages (early/developmental/late) were registered. During the early-stage, EHP was not consistently detected, and a high diversity of potentially beneficial microorganisms related to nutrient assimilation were found. In the development-stage, most of the shrimp start to register a high infection intensity related to a decrease in beneficial microorganisms and an increase in opportunistic/pathogenic fungi. During late-stage, animals displayed different infection intensities, showed a displacement of beneficial microorganisms by opportunistic/pathogenic bacteria and fungi related to pathogen infection processes and depletion of energetic reserves. The degenerative cyclic pattern of EHP infection and its effects on beneficial microorganisms and beneficial functions of the shrimp hepatopancreas microbiome are discussed.
Keywords	Medicine ; R ; Science ; Q
Subject code	630
Language	English
Publishing date	2022-08-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Application of PCR-based diagnostic tools that target Enterocytozoon hepatopenaei for the molecular detection of a Vittaforma-like microsporidium that infects Penaeus vannamei from Costa Rica

Peña-Navarro, Nelson / López-Carvallo, Antonio / Chacón Perez, Brandon / Cruz-Flores, Roberto

Journal of Invertebrate Pathology. 20232023 Sept. 08, July 08, v. 200 p.107958-

2023

Abstract	Several PCR methodologies are available for the detection of Enterocytozoon hepatopenaei (EHP) that target the SSU rRNA gene. However, these methodologies are reported as unsuitable for the detection of EHP due to specificity issues. Here, we report the applicability of two commonly used SSU rRNA methodologies for the detection of additional microsporidia from the genus Vittaforma that is present in cultured Penaeus vannamei from Costa Rica. The molecular detection of DNA of the novel microsporidia can only be achieved using SSU rRNA targeting methodologies and does not cross-react with the highly specific spore wall protein gene PCR detection method.
Keywords	DNA ; Enterocytozoon ; Litopenaeus vannamei ; genes ; invertebrates ; polymerase chain reaction ; spores ; Costa Rica ; Vittaforma sp. ; Enterocytozoon hepatopenaei ; Microsporidia ; Shrimp ; Penaeus vannamei
Language	English
Dates of publication	2023-0708
Publishing place	Elsevier Inc.
Document type	Article ; Online
ZDB-ID	390885-9
ISSN	1096-0805 ; 0022-2011
ISSN (online)	1096-0805
ISSN	0022-2011
DOI	10.1016/j.jip.2023.107958
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Mimicking the Tumor Niche: Methods for Isolation, Culture, and Characterization of Cancer Stem Cells and Multicellular Spheroids.

De Lara-Peña, Laura / Farace, Cristiano / Pisano, Andrea / de Andrés, Julia López / Fenu, Grazia / Etzi, Federica / Griñán-Lisón, Carmen / Marchal, Juan Antonio / Madeddu, Roberto

Methods in molecular biology (Clifton, N.J.)

2024 Volume 2777, Page(s) 145–161

Abstract: Cancer stem cells (CSCs) play a significant role in driving several tumor hallmarks. Their behavior and tumor progression are strictly related to the tumor microenvironment (TME). The dynamic interplay between CSCs and TME drives metastasis, ... ...

Abstract	Cancer stem cells (CSCs) play a significant role in driving several tumor hallmarks. Their behavior and tumor progression are strictly related to the tumor microenvironment (TME). The dynamic interplay between CSCs and TME drives metastasis, chemoresistance, and disease relapse. In this chapter, we describe different techniques and protocols for isolating, culturing, and characterizing CSCs and we explain the methodology for the culture of multicellular spheroids comprising CSCs.
MeSH term(s)	Humans ; Spheroids, Cellular/pathology ; Neoplasms/pathology ; Neoplastic Stem Cells/pathology ; Tumor Microenvironment
Language	English
Publishing date	2024-03-13
Publishing country	United States
Document type	Journal Article
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/978-1-0716-3730-2_11
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Article ; Online: Uncovering the Dissipation of Chlorantraniliprole in Tomatoes: Identifying Transformation Products (TPs) and Coformulants in Greenhouse and Laboratory Studies by UHPLC-Q-Orbitrap-MS and GC-Q-Orbitrap-MS.

Maldonado-Reina, Antonio Jesús / López-Ruiz, Rosalía / Marín Sáez, Jesús / Romero-González, Roberto / Marín-Membrive, Patricia / Garrido-Frenich, Antonia

Journal of agricultural and food chemistry

2023 Volume 71, Issue 19, Page(s) 7230–7238

Abstract: The present study addressed the dissipation of the insecticide chlorantraniliprole in tomatoes treated with Altacor 35 WG under laboratory and greenhouse conditions, as well as the identification of transformation products (TPs) and coformulants, ... ...

Abstract	The present study addressed the dissipation of the insecticide chlorantraniliprole in tomatoes treated with Altacor 35 WG under laboratory and greenhouse conditions, as well as the identification of transformation products (TPs) and coformulants, performing suspect screening analysis. Analyses were performed by ultra-high-performance liquid and gas chromatography coupled to quadrupole-Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS and GC-Q-Orbitrap-MS). In all cases, chlorantraniliprole was fitted to a biphasic kinetic model, with
MeSH term(s)	Gas Chromatography-Mass Spectrometry ; Chromatography, High Pressure Liquid/methods ; Solanum lycopersicum ; Mass Spectrometry/methods
Chemical Substances	chlorantranilipole (622AK9DH9G)
Language	English
Publishing date	2023-05-08
Publishing country	United States
Document type	Journal Article
ZDB-ID	241619-0
ISSN	1520-5118 ; 0021-8561
ISSN (online)	1520-5118
ISSN	0021-8561
DOI	10.1021/acs.jafc.3c00816
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