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Article ; Online: Production and Characterization of Two Specific ZIKV Antigens Based on Bioinformatic Analysis and Serological Screening.

Ribeiro Mota Souza, Rafael / Campos, Gubio Soares / Hughes Carvalho, Rejane / Brandão Peixoto, Isabela / Santos Galante, Rafaela / Santana Moreira, Luan / Beutinger Marchioro, Silvana / José Meyer Nascimento, Roberto / Sardi, Silvia Ines

Immunological investigations

2023 Volume 52, Issue 4, Page(s) 454–466

Abstract: ... that the region 146-182 of ZIKV's E protein, expressed in tandem, was not reactive despite the predictive ... sensitivity and specificity observed by computer analyses. On the other hand, the non-denatured fraction 220-352 of ZIKV's NS1 ... of serological diagnosis. We also provided data that suggest the non-applicability of the region 146-182 of ZIKV's protein ...

Abstract	Background: The high structural similarity between the Zika virus (ZIKV) and other flaviviruses, such as Dengue Virus (DENV), complicates the identification of the infecting virus due to the occurrence of cross-reactions in serological assays. This phenomenon has increased the demand for more specific antigens for immunodiagnostic applications. Methods: The present work aimed to identify specific regions of ZIKV and produce unique antigens through computational methods, molecular and microbiological techniques. Results: Based on the computational analysis we successfully expressed two recombinant proteins derived from specific regions of the ZIKV. Through serological assays using characterized sera, we observed that the region 146-182 of ZIKV's E protein, expressed in tandem, was not reactive despite the predictive sensitivity and specificity observed by computer analyses. On the other hand, the non-denatured fraction 220-352 of ZIKV's NS1 showed greater specificity to IgG+ sera of ZIKV by dot blot and western blot, which highlights its properties as a possible tool in the diagnosis of ZIKV. Conclusion: These findings demonstrate that ZIKV NS1 fraction 220-352 is a potential tool that may be applied in the development of serological diagnosis. We also provided data that suggest the non-applicability of the region 146-182 of ZIKV's protein E in serological assays despite previous indications about its potential based on computational analysis.
MeSH term(s)	Humans ; Zika Virus/genetics ; Zika Virus Infection/diagnosis ; Dengue ; Dengue Virus/genetics ; Enzyme-Linked Immunosorbent Assay/methods ; Antibodies, Viral ; Serologic Tests/methods ; Cross Reactions
Chemical Substances	Antibodies, Viral
Language	English
Publishing date	2023-04-10
Publishing country	England
Document type	Journal Article
ZDB-ID	632565-8
ISSN	1532-4311 ; 0882-0139
ISSN (online)	1532-4311
ISSN	0882-0139
DOI	10.1080/08820139.2023.2195432
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Article ; Online: Zika Virus Outbreak, Bahia, Brazil.

Campos, Gubio S / Bandeira, Antonio C / Sardi, Silvia I

Emerging infectious diseases

2015 Volume 21, Issue 10, Page(s) 1885–1886

MeSH term(s)	Brazil/epidemiology ; Geography ; Humans ; Zika Virus Infection/epidemiology
Language	English
Publishing date	2015-09-10
Publishing country	United States
Document type	Letter ; Research Support, Non-U.S. Gov't
ZDB-ID	1380686-5
ISSN	1080-6059 ; 1080-6040
ISSN (online)	1080-6059
ISSN	1080-6040
DOI	10.3201/eid2110.150847
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Article ; Online: Genomic monitoring unveils a high prevalence of SARS-CoV 2 Omicron Variant in vaccine breakthrough cases in Bahia, Brazil

Campos, Gubio Soares / Giovanetti, Marta / de Moraes, Laise / da Hora, Helena S / Alcantara, Keila Motta / Sardi, Silvia Ines

medRxiv

Abstract: Genome sequencing proved to be an excellent tool to monitor the molecular epidemiology of the disease caused by SARS-CoV-2, i.e., coronavirus disease (COVID-19). Some reports of infected, vaccinated individuals have aroused great interest because they ... ...

Abstract	Genome sequencing proved to be an excellent tool to monitor the molecular epidemiology of the disease caused by SARS-CoV-2, i.e., coronavirus disease (COVID-19). Some reports of infected, vaccinated individuals have aroused great interest because they are primarily being infected with circulating variants of concern (VOCs). To investigate the cases of infected, vaccinated individuals in Salvador, Bahia, Brazil, we performed genomic monitoring to estimate the magnitude of the different VOCs in these cases. Nasopharyngeal swabs from infected (symptomatic and asymptomatic), fully vaccinated individuals (n=29) who were of varying age and had RT-qPCR Ct values of ≤30 were subjected to viral sequencing using Nanopore technology. Our analysis revealed that the Omicron variant was found in 99% of cases and that only one case was due to the Delta variant. Infected, fully vaccinated patients have a favorable clinical prognosis; however, within the community, they become viral carriers with the aggravating factor of viral dissemination of VOCs not neutralized by the vaccines.
Keywords	covid19
Language	English
Publishing date	2022-02-21
Publisher	Cold Spring Harbor Laboratory Press
Document type	Article ; Online
DOI	10.1101/2022.02.16.22271059
Database	COVID19

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Article ; Online: Zika Virus Outbreak, Bahia, Brazil

Gubio S. Campos / Antonio C. Bandeira / Silvia I. Sardi

Emerging Infectious Diseases, Vol 21, Iss 10, Pp 1885-

2015 Volume 1886

Keywords	Zika virus ; viruses ; outbreak ; maculopapular rash ; detection ; diagnosis ; Medicine ; R ; Infectious and parasitic diseases ; RC109-216
Language	English
Publishing date	2015-10-01T00:00:00Z
Publisher	Centers for Disease Control and Prevention
Document type	Article ; Online
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Article ; Online: Analyses of Early ZIKV Genomes Are Consistent with Viral Spread from Northeast Brazil to the Americas.

de Moraes, Laise / Portilho, Moyra M / Vrancken, Bram / Van den Broeck, Frederik / Santos, Luciane Amorim / Cucco, Marina / Tauro, Laura B / Kikuti, Mariana / Silva, Monaise M O / Campos, Gúbio S / Reis, Mitermayer G / Barral, Aldina / Barral-Netto, Manoel / Boaventura, Viviane Sampaio / Vandamme, Anne-Mieke / Theys, Kristof / Lemey, Philippe / Ribeiro, Guilherme S / Khouri, Ricardo

Viruses

2023 Volume 15, Issue 6

Abstract: The Americas, particularly Brazil, were greatly impacted by the widespread Zika virus (ZIKV) outbreak in 2015 and 2016. Efforts were made to implement genomic surveillance of ZIKV as part of the public health responses. The accuracy of spatiotemporal ... ...

Abstract	The Americas, particularly Brazil, were greatly impacted by the widespread Zika virus (ZIKV) outbreak in 2015 and 2016. Efforts were made to implement genomic surveillance of ZIKV as part of the public health responses. The accuracy of spatiotemporal reconstructions of the epidemic spread relies on the unbiased sampling of the transmission process. In the early stages of the outbreak, we recruited patients exhibiting clinical symptoms of arbovirus-like infection from Salvador and Campo Formoso, Bahia, in Northeast Brazil. Between May 2015 and June 2016, we identified 21 cases of acute ZIKV infection and subsequently recovered 14 near full-length sequences using the amplicon tiling multiplex approach with nanopore sequencing. We performed a time-calibrated discrete phylogeographic analysis to trace the spread and migration history of the ZIKV. Our phylogenetic analysis supports a consistent relationship between ZIKV migration from Northeast to Southeast Brazil and its subsequent dissemination beyond Brazil. Additionally, our analysis provides insights into the migration of ZIKV from Brazil to Haiti and the role Brazil played in the spread of ZIKV to other countries, such as Singapore, the USA, and the Dominican Republic. The data generated by this study enhances our understanding of ZIKV dynamics and supports the existing knowledge, which can aid in future surveillance efforts against the virus.
MeSH term(s)	Humans ; Zika Virus/genetics ; Zika Virus Infection ; Brazil/epidemiology ; Phylogeny ; Americas/epidemiology
Language	English
Publishing date	2023-05-25
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v15061236
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Article ; Online: High-Quality Resolution of the Outbreak-Related Zika Virus Genome and Discovery of New Viruses Using Ion Torrent-Based Metatranscriptomics.

I Sardi, Silvia / H Carvalho, Rejane / C Pacheco, Luis G / P D Almeida, João P / M D A Belitardo, Emilia M / S Pinheiro, Carina / S Campos, Gúbio / R G R Aguiar, Eric

Viruses

2020 Volume 12, Issue 7

Abstract: Arboviruses, including ... ...

Abstract	Arboviruses, including the
MeSH term(s)	Arboviruses/genetics ; Disease Outbreaks ; Gene Expression Profiling/methods ; Genome, Viral/genetics ; High-Throughput Nucleotide Sequencing/methods ; Humans ; Mosquito Vectors/virology ; Nidovirales/genetics ; Phylogeny ; Polymerase Chain Reaction ; Zika Virus/genetics ; Zika Virus Infection/epidemiology ; Zika Virus Infection/virology
Keywords	covid19
Language	English
Publishing date	2020-07-21
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2516098-9
ISSN	1999-4915 ; 1999-4915
ISSN (online)	1999-4915
ISSN	1999-4915
DOI	10.3390/v12070782
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Article ; Online: Risk of chronic arthralgia and impact of pain on daily activities in a cohort of patients with chikungunya virus infection from Brazil.

Silva, Monaíse M O / Kikuti, Mariana / Anjos, Rosângela O / Portilho, Moyra M / Santos, Viviane C / Gonçalves, Thaiza S F / Tauro, Laura B / Moreira, Patrícia S S / Jacob-Nascimento, Leile C / Santana, Perla M / Campos, Gúbio S / Siqueira, André M / Kitron, Uriel / Reis, Mitermayer G / Ribeiro, Guilherme S

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

2021 Volume 105, Page(s) 608–616

Abstract: Objectives: To investigate risk factors for persistent arthralgia in patients with chikungunya, and describe its impact on daily activities.: Methods: From September 2014 to July 2016, a surveillance study enrolled patients with acute febrile illness ...

Abstract	Objectives: To investigate risk factors for persistent arthralgia in patients with chikungunya, and describe its impact on daily activities. Methods: From September 2014 to July 2016, a surveillance study enrolled patients with acute febrile illness in Salvador, Brazil, and detected those with chikungunya virus infection using IgM enzyme-linked immunosorbent assay or reverse transcriptase polymerase chain reaction. Telephone follow-ups were performed to ascertain the progression of disease. Results: Of 153 followed cases, 65 (42.5%) reported chronic arthralgia that lasted >3 months, and 47 (30.7%) were still symptomatic at the time of the interview (approximately 1.5 years after symptom onset). Limitations in daily activities and mental distress were reported by 93.8% and 61.5% of those with chronic arthralgia, respectively. Female sex [risk ratio (RR) 1.79, 95% confidence interval (CI) 1.95-2.69] and age (RR 1.02 for each 1-year increase, 95% CI 1.01-1.03) were independent risk factors for chronic arthralgia. Chronic arthralgia was not associated with co-infection with dengue virus (RR 0.97, 95% CI 0.48-1.94) or chikungunya viral load at diagnosis (median chikungunya virus RNA of 5.60 and 5.52 log Conclusions: These findings reinforce the high frequency of chronic chikungunya arthralgia, and highlight the substantial disability associated with the persistence of pain. Development of novel strategies to mitigate the transmission of chikungunya virus and to provide long-term medical assistance for patients with chikungunya are needed urgently.
MeSH term(s)	Adolescent ; Adult ; Arthralgia/epidemiology ; Arthralgia/etiology ; Arthralgia/virology ; Brazil/epidemiology ; Chikungunya Fever/complications ; Chikungunya Fever/epidemiology ; Chikungunya Fever/virology ; Chikungunya virus/genetics ; Chikungunya virus/immunology ; Child ; Child, Preschool ; Chronic Pain/epidemiology ; Chronic Pain/etiology ; Chronic Pain/virology ; Cohort Studies ; Enzyme-Linked Immunosorbent Assay ; Female ; Humans ; Infant ; Male ; Middle Aged ; Odds Ratio ; Risk Factors ; Young Adult
Language	English
Publishing date	2021-03-05
Publishing country	Canada
Document type	Journal Article
ZDB-ID	1331197-9
ISSN	1878-3511 ; 1201-9712
ISSN (online)	1878-3511
ISSN	1201-9712
DOI	10.1016/j.ijid.2021.03.003
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Article ; Online: Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19.

Campos, Gubio S / Sardi, Silvia I / Falcao, Melissa B / Belitardo, Emilia M M A / Rocha, Danilo J P G / Rolo, Carolina A / Menezes, Aline D / Pinheiro, Carina S / Carvalho, Rejane H / Almeida, João P P / Aguiar, Eric R G R / Pacheco, Luis G C

Journal of virological methods

2020 Volume 282, Page(s) 113888

Abstract: Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use ...

Abstract	Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.
Keywords	covid19
Language	English
Publishing date	2020-05-21
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	8013-5
ISSN	1879-0984 ; 0166-0934
ISSN (online)	1879-0984
ISSN	0166-0934
DOI	10.1016/j.jviromet.2020.113888
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Article ; Online: MTOR gene variants are associated with severe COVID-19 outcomes: A multicenter study.

Tosta, Bruna Ramos / de Almeida, Ingrid Marins / da Cruz Pena, Laiane / Dos Santos Silva, Hatilla / Reis-Goes, Fabiane S / Silva, Nívia N / Cruz, João Victor Andrade / Dos Anjos Silva, Mailane / de Araújo, Jéssica Francisco / Rodrigues, Juliana Lopes / Oliveira, Gabriella / Figueiredo, Ricardo Gassmann / Vaz, Sara Nunes / Montaño-Castellón, Iris / Santana, Daniele / de Lima Beltrão, Fabyan Esberard / Carneiro, Valdirene Leão / Campos, Gubio Soares / Brites, Carlos /

Fortuna, Vitor / Figueiredo, Camila Alexandrina / Trindade, Soraya Castro / Ramos, Helton Estrela / Costa, Ryan Dos Santos

International immunopharmacology

2023 Volume 125, Issue Pt B, Page(s) 111155

Abstract: Background: The worst outcomes linked to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been attributed to the cytokine storm, which contributes significantly to the immunopathogenesis of the disease. The mammalian target of ...

Abstract	Background: The worst outcomes linked to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection have been attributed to the cytokine storm, which contributes significantly to the immunopathogenesis of the disease. The mammalian target of rapamycin (mTOR) pathway is essential for orchestrating innate immune cell defense including cytokine production and is dysregulated in severe Coronavirus Disease 2019 (COVID-19) individuals. The individual genetic background might play a role in the exacerbated immune response. Objective: In this study, we aimed to investigate the association between MTOR genetic variants and COVID-19 outcomes. Methods: This study enrolled groups of individuals with severe (n = 285) and mild (n = 207) COVID-19 from Brazilian states. The MTOR variants, rs1057079 and rs2536, were genotyped. A logistic regression analysis and Kaplan-Meier survival curves were performed. We applied a genotyping risk score to estimate the cumulative contribution of the risk alleles. Tumor necrosis factor (TNF) and interleukin-6 (IL-6) plasma levels were also measured. Results: The T allele of the MTOR rs1057079 variant was associated with a higher likelihood of developing the most severe form of COVID-19. In addition, higher levels of IL-6 and COVID-19 death was linked to the T allele of the rs2536 variant. These variants exhibited a cumulative risk when inherited collectively. Conclusions: These results show a potential pathogenetic role of MTOR gene variants and may be useful for predicting severe outcomes following COVID-19 infection, resulting in a more effective allocation of health resources.
MeSH term(s)	Genetic Variation ; Humans ; COVID-19/genetics ; COVID-19/immunology ; COVID-19/mortality ; COVID-19/pathology ; Patient Acuity ; Case-Control Studies ; Male ; Female ; Adult ; Middle Aged ; Aged ; Survival Analysis ; Cytokines/blood ; TOR Serine-Threonine Kinases/genetics
Chemical Substances	Cytokines ; MTOR protein, human (EC 2.7.1.1) ; TOR Serine-Threonine Kinases (EC 2.7.11.1)
Language	English
Publishing date	2023-11-09
Publishing country	Netherlands
Document type	Multicenter Study ; Journal Article
ZDB-ID	2043785-7
ISSN	1878-1705 ; 1567-5769
ISSN (online)	1878-1705
ISSN	1567-5769
DOI	10.1016/j.intimp.2023.111155
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Zs.A 5408: Show issues

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Article ; Online: A localized outbreak of Chikungunya virus in Salvador, Bahia, Brazil.

Tauro, Laura B / Cardoso, Cristiane W / Souza, Raquel L / Nascimento, Leile Cj / Santos, Daniela R Dos / Campos, Gubio S / Sardi, Silvia / Reis, Olivete B Dos / Reis, Mitermayer G / Kitron, Uriel / Ribeiro, Guilherme S

Memorias do Instituto Oswaldo Cruz

2019 Volume 114, Page(s) e180597

Abstract: A localized Chikungunya virus (CHIKV; East/Central/South African genotype) outbreak (50 cases, 70% laboratory-confirmed; attack rate: 5.3 confirmed cases/100 people) occurred in a Salvador, Brazil neighborhood, between Apr-Jun/2017. Highly clustered ... ...

Abstract	A localized Chikungunya virus (CHIKV; East/Central/South African genotype) outbreak (50 cases, 70% laboratory-confirmed; attack rate: 5.3 confirmed cases/100 people) occurred in a Salvador, Brazil neighborhood, between Apr-Jun/2017. Highly clustered cases in space and time, mostly along a single street, highlight an increased risk of CHIKV transmission among pockets of susceptible populations. This finding underscores the need for ongoing local level surveillance for arboviral outbreaks.
MeSH term(s)	Adult ; Brazil/epidemiology ; Chikungunya Fever/diagnosis ; Chikungunya Fever/epidemiology ; Chikungunya Fever/virology ; Chikungunya virus/genetics ; Chikungunya virus/immunology ; Disease Outbreaks/statistics & numerical data ; Enzyme-Linked Immunosorbent Assay ; Female ; Genotype ; Humans ; Male ; Middle Aged ; Phylogeny ; Polymerase Chain Reaction ; Seasons ; Young Adult
Language	English
Publishing date	2019-02-28
Publishing country	Brazil
Document type	Journal Article
ZDB-ID	953293-6
ISSN	1678-8060 ; 0074-0276
ISSN (online)	1678-8060
ISSN	0074-0276
DOI	10.1590/0074-02760180597
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