LIVIVO - Search results -

Search results

Result 1 - 10 of total 143

Search options

Article ; Online: TIAM-1 differentially regulates dendritic and axonal microtubule organization in patterning neuronal development through its multiple domains.

Lin, Chih-Hsien / Chen, Ying-Chun / Chan, Shih-Peng / Ou, Chan-Yen

2022 Volume 18, Issue 10, Page(s) e1010454

Abstract: Axon and dendrite development require the cooperation of actin and microtubule cytoskeletons. Microtubules form a well-organized network to direct polarized trafficking and support neuronal processes formation with distinct actin structures. However, it ... ...

Abstract	Axon and dendrite development require the cooperation of actin and microtubule cytoskeletons. Microtubules form a well-organized network to direct polarized trafficking and support neuronal processes formation with distinct actin structures. However, it is largely unknown how cytoskeleton regulators differentially regulate microtubule organization in axon and dendrite development. Here, we characterize the role of actin regulators in axon and dendrite development and show that the RacGEF TIAM-1 regulates dendritic patterns through its N-terminal domains and suppresses axon growth through its C-terminal domains. TIAM-1 maintains plus-end-out microtubule orientation in posterior dendrites and prevents the accumulation of microtubules in the axon. In somatodendritic regions, TIAM-1 interacts with UNC-119 and stabilizes the organization between actin filaments and microtubules. UNC-119 is required for TIAM-1 to control axon growth, and its expression levels determine axon length. Taken together, TIAM-1 regulates neuronal microtubule organization and patterns axon and dendrite development respectively through its different domains.
MeSH term(s)	Dendrites/genetics ; Dendrites/metabolism ; Actins/metabolism ; Axons/metabolism ; Microtubules/metabolism ; Neurogenesis/genetics
Chemical Substances	Actins
Language	English
Publishing date	2022-10-12
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2186725-2
ISSN	1553-7404 ; 1553-7390
ISSN (online)	1553-7404
ISSN	1553-7390
DOI	10.1371/journal.pgen.1010454
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Article ; Online: Author Correction: Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.

Liu, Wei-Liang / Hsu, Chia-Wei / Chan, Shih-Peng / Yen, Pei-Shi / Su, Matthew P / Li, Jian-Chiuan / Li, Hsing-Han / Cheng, Lie / Tang, Cheng-Kang / Ko, Shih-Hsun / Tsai, Huai-Kuang / Tsai, Zing Tsung-Yeh / Akbari, Omar S / Failloux, Anna-Bella / Chen, Chun-Hong

Scientific reports

2022 Volume 12, Issue 1, Page(s) 754

Language	English
Publishing date	2022-01-10
Publishing country	England
Document type	Published Erratum
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-05054-9
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Author Correction

Wei-Liang Liu / Chia-Wei Hsu / Shih-Peng Chan / Pei-Shi Yen / Matthew P. Su / Jian-Chiuan Li / Hsing-Han Li / Lie Cheng / Cheng-Kang Tang / Shih-Hsun Ko / Huai-Kuang Tsai / Zing Tsung-Yeh Tsai / Omar S. Akbari / Anna-Bella Failloux / Chun-Hong Chen

Scientific Reports, Vol 12, Iss 1, Pp 1-

Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus

2022 Volume 1

Keywords	Medicine ; R ; Science ; Q
Language	English
Publishing date	2022-01-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: A novel function for the DEAD-box RNA helicase DDX-23 in primary microRNA processing in Caenorhabditis elegans.

Chu, Yu-De / Chen, Hsin-Kai / Huang, Tao / Chan, Shih-Peng

Developmental biology

2016 Volume 409, Issue 2, Page(s) 459–472

Abstract: Primary microRNAs (pri-miRNAs) are cleaved by the nuclear RNase III Drosha to produce hairpin-shaped precursor miRNAs (pre-miRNAs). In humans, this process is known to be facilitated by the DEAD-box helicases p68 (DDX5) and p72 (DDX17). In this study, we ...

Abstract	Primary microRNAs (pri-miRNAs) are cleaved by the nuclear RNase III Drosha to produce hairpin-shaped precursor miRNAs (pre-miRNAs). In humans, this process is known to be facilitated by the DEAD-box helicases p68 (DDX5) and p72 (DDX17). In this study, we performed a candidate-based RNAi screen in C. elegans to identify DEAD/H-box proteins involved in miRNA biogenesis. In a let-7(mg279) sensitized genetic background, knockdown of a homolog of yeast splicing factor Prp28p, DDX-23, or a homolog of human helicases p68 and p72, DDX-17, enhanced let-7 loss-of-function phenotypes, suggesting that these helicases play a role in let-7 processing and/or function. In both ddx-23(RNAi) and ddx-17(RNAi), levels of mature let-7 were decreased while pri-let-7 was found to accumulate, indicating that the helicases likely act at the level of pri-let-7 processing. DDX-23 and DDX-17 were also required for the biogenesis of other known heterochronic miRNAs, including lin-4 and the let-7 family members miR-48, miR-84 and miR-241. Their function was not confined to the heterochronic pathway, however, since they were both necessary for down-regulation of cog-1 by the spatial patterning miRNA, lsy-6. Here, we present a novel function for C. elegans DDX-23 in pri-miRNA processing, and also suggest a conserved role for DDX-17 in this process.
MeSH term(s)	Animals ; Caenorhabditis elegans/enzymology ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/metabolism ; Cell Nucleus/metabolism ; DEAD-box RNA Helicases/metabolism ; Gene Expression Regulation ; Green Fluorescent Proteins/metabolism ; Larva/genetics ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Mutation/genetics ; RNA Interference ; RNA Processing, Post-Transcriptional/genetics
Chemical Substances	Caenorhabditis elegans Proteins ; MicroRNAs ; let-7 microRNA, C elegans ; Green Fluorescent Proteins (147336-22-9) ; DDX-23 protein, C elegans (EC 2.7.7.-) ; DEAD-box RNA Helicases (EC 3.6.4.13)
Language	English
Publishing date	2016-01-15
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2015.11.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 508: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: phiC31 integrase for recombination-mediated single-copy insertion and genome manipulation in Caenorhabditis elegans.

Yang, Fang-Jung / Chen, Chiao-Nung / Chang, Tiffany / Cheng, Ting-Wei / Chang, Ni-Chen / Kao, Chia-Yi / Lee, Chih-Chi / Huang, Yu-Ching / Hsu, Jung-Chen / Li, Jengyi / Lu, Meiyeh J / Chan, Shih-Peng / Wang, John

Genetics

2021 Volume 220, Issue 2

Abstract: Caenorhabditis elegans benefits from a large set of tools for genome manipulation. Yet, the precise single-copy insertion of very large DNA constructs (>10 kb) and the generation of inversions are still challenging. Here, we adapted the phiC31 integrase ... ...

Abstract	Caenorhabditis elegans benefits from a large set of tools for genome manipulation. Yet, the precise single-copy insertion of very large DNA constructs (>10 kb) and the generation of inversions are still challenging. Here, we adapted the phiC31 integrase system for C. elegans. We generated an integrated phiC31 integrase expressing strain flanked by attP sites that serves as a landing pad for integration of transgenes by recombination-mediated cassette exchange (RCME). This strain is unc-119(-) so RMCE integrants can be produced simply by injection of a plasmid carrying attB sites flanking unc-119(+) and the gene(s) of interest. Additionally, phiC31 integrase is removed concomitantly with integration, eliminating the need to outcross away the integrase. Integrations were obtained for insert sizes up to ∼33.4 kb. Taking advantage of this integration method we establish a dual-color fluorescent operon reporter system able to study post-transcriptional regulation of mRNA. Last, we show that large chromosomal segments can be inverted using phiC31 integrase. Thus, the phiC31 integrase system should be a useful addition to the C. elegans toolkit.
MeSH term(s)	Animals ; Bacteriophages/genetics ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/genetics ; Integrases/genetics ; Nerve Tissue Proteins/genetics ; Recombination, Genetic ; Transgenes
Chemical Substances	Caenorhabditis elegans Proteins ; Nerve Tissue Proteins ; UNC-119 protein, C elegans ; Integrases (EC 2.7.7.-)
Language	English
Publishing date	2021-11-17
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2167-2
ISSN	1943-2631 ; 0016-6731
ISSN (online)	1943-2631
ISSN	0016-6731
DOI	10.1093/genetics/iyab206
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 767: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Transgenic refractory Aedes aegypti lines are resistant to multiple serotypes of dengue virus.

Scientific reports

2021 Volume 11, Issue 1, Page(s) 23865

Abstract: The areas where dengue virus (DENV) is endemic have expanded rapidly, driven in part by the global spread of Aedes species, which act as disease vectors. DENV replicates in the mosquito midgut and is disseminated to the mosquito's salivary glands for ... ...

Abstract	The areas where dengue virus (DENV) is endemic have expanded rapidly, driven in part by the global spread of Aedes species, which act as disease vectors. DENV replicates in the mosquito midgut and is disseminated to the mosquito's salivary glands for amplification. Thus, blocking virus infection or replication in the tissues of the mosquito may be a viable strategy for reducing the incidence of DENV transmission to humans. Here we used the mariner Mos1 transposase to create an Aedes aegypti line that expresses virus-specific miRNA hairpins capable of blocking DENV replication. These microRNA are driven by the blood-meal-inducible carboxypeptidase A promoter or by the polyubiquitin promoter. The transgenic mosquitoes exhibited significantly lower infection rates and viral titers for most DENV serotypes 7 days after receiving an infectious blood meal. The treatment was also effective at day 14 post infection after a second blood meal had been administered. In viral transmission assay, we found there was significantly reduced transmission in these lines. These transgenic mosquitoes were effective in silencing most of the DENV genome; such an approach may be employed to control a dengue fever epidemic.
MeSH term(s)	Aedes/genetics ; Aedes/virology ; Animals ; Animals, Genetically Modified ; Cell Line ; Cricetinae ; Cricetulus ; Dengue/prevention & control ; Dengue/transmission ; Dengue Virus/genetics ; Dengue Virus/pathogenicity ; Fibroblasts/virology ; Mosquito Control/methods ; Mosquito Vectors/genetics ; Mosquito Vectors/virology ; RNA, Small Interfering/genetics ; RNA, Small Interfering/metabolism ; Serogroup ; Transposases/genetics ; Transposases/metabolism ; Viral Load
Chemical Substances	RNA, Small Interfering ; Transposases (EC 2.7.7.-)
Language	English
Publishing date	2021-12-13
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-03229-4
Database	MEDical Literature Analysis and Retrieval System OnLINE

Order via subito

Article ; Online: Ribosomal protein RPS-14 modulates let-7 microRNA function in Caenorhabditis elegans.

Chan, Shih-Peng / Slack, Frank J

Developmental biology

2009 Volume 334, Issue 1, Page(s) 152–160

Abstract: The let-7 microRNA (miRNA) regulates developmental timing at the larval-to-adult transition in Caenorhabditis elegans. Dysregulation of let-7 results in irregular hypodermal and vulval development. Disrupted let-7 function is also a feature of human lung ...

Abstract	The let-7 microRNA (miRNA) regulates developmental timing at the larval-to-adult transition in Caenorhabditis elegans. Dysregulation of let-7 results in irregular hypodermal and vulval development. Disrupted let-7 function is also a feature of human lung cancer. However, little is known about the mechanism and co-factors of let-7. Here we demonstrate that ribosomal protein RPS-14 is able to modulate let-7 function in C. elegans. The RPS-14 protein co-immunoprecipitated with the nematode Argonaute homolog, ALG-1. Reduction of rps-14 gene expression by RNAi suppressed the aberrant vulva and hypodermis development phenotypes of let-7(n2853) mutant animals and the mis-regulation of a reporter bearing the lin-41 3'UTR, a well established let-7 target. Our results indicate an interactive relationship between let-7 miRNA function and ribosomal protein RPS-14 in regulation of terminal differentiation that may help in understanding the mechanism of translational control by miRNAs.
MeSH term(s)	Animals ; Animals, Genetically Modified ; Body Patterning/genetics ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/genetics ; Caenorhabditis elegans Proteins/metabolism ; Electrophoretic Mobility Shift Assay ; Gene Expression Regulation, Developmental ; Genes, Helminth ; MicroRNAs/genetics ; MicroRNAs/physiology ; Mutation ; Phenotype ; Ribosomal Proteins/genetics ; Ribosomal Proteins/metabolism
Chemical Substances	Caenorhabditis elegans Proteins ; MicroRNAs ; Ribosomal Proteins ; Rps-14 protein, C elegans ; let-7 microRNA, C elegans
Language	English
Publishing date	2009-07-21
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2009.07.011
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.B 508: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: A novel function for the DEAD-box RNA helicase DDX-23 in primary microRNA processing in Caenorhabditis elegans

Chu, Yu-De / Hsin-Kai Chen / Tao Huang / Shih-Peng Chan

Developmental biology. 2016 Jan. 15, v. 409

2016

Abstract	Primary microRNAs (pri-miRNAs) are cleaved by the nuclear RNase III Drosha to produce hairpin-shaped precursor miRNAs (pre-miRNAs). In humans, this process is known to be facilitated by the DEAD-box helicases p68 (DDX5) and p72 (DDX17). In this study, we performed a candidate-based RNAi screen in C. elegans to identify DEAD/H-box proteins involved in miRNA biogenesis. In a let-7(mg279) sensitized genetic background, knockdown of a homolog of yeast splicing factor Prp28p, DDX-23, or a homolog of human helicases p68 and p72, DDX-17, enhanced let-7 loss-of-function phenotypes, suggesting that these helicases play a role in let-7 processing and/or function. In both ddx-23(RNAi) and ddx-17(RNAi), levels of mature let-7 were decreased while pri-let-7 was found to accumulate, indicating that the helicases likely act at the level of pri-let-7 processing. DDX-23 and DDX-17 were also required for the biogenesis of other known heterochronic miRNAs, including lin-4 and the let-7 family members miR-48, miR-84 and miR-241. Their function was not confined to the heterochronic pathway, however, since they were both necessary for down-regulation of cog-1 by the spatial patterning miRNA, lsy-6. Here, we present a novel function for C. elegans DDX-23 in pri-miRNA processing, and also suggest a conserved role for DDX-17 in this process.
Keywords	Caenorhabditis elegans ; DEAD-box RNA helicases ; RNA interference ; biogenesis ; genetic background ; humans ; loss-of-function mutation ; microRNA ; phenotype ; proteins ; ribonucleases ; yeasts
Language	English
Dates of publication	2016-0115
Size	p. 459-472.
Publishing place	Elsevier Inc.
Document type	Article
ZDB-ID	1114-9
ISSN	1095-564X ; 0012-1606
ISSN (online)	1095-564X
ISSN	0012-1606
DOI	10.1016/j.ydbio.2015.11.011
Database	NAL-Catalogue (AGRICOLA)

In stock of ZB MED Bonn / Germany

Z 76/715: Show issues

Order via subito

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: Loss of Fis1 impairs proteostasis during skeletal muscle aging in Drosophila.

Lee, Tai-Ting / Chen, Po-Lin / Su, Matthew P / Li, Jian-Chiuan / Chang, Yi-Wen / Liu, Rei-Wen / Juan, Hsueh-Fen / Yang, Jinn-Moon / Chan, Shih-Peng / Tsai, Yu-Chen / von Stockum, Sophia / Ziviani, Elena / Kamikouchi, Azusa / Wang, Horng-Dar / Chen, Chun-Hong

Aging cell

2021 Volume 20, Issue 6, Page(s) e13379

Abstract: Increased levels of dysfunctional mitochondria within skeletal muscle are correlated with numerous age-related physiopathological conditions. Improving our understanding of the links between mitochondrial function and muscle proteostasis, and the role ... ...

Abstract	Increased levels of dysfunctional mitochondria within skeletal muscle are correlated with numerous age-related physiopathological conditions. Improving our understanding of the links between mitochondrial function and muscle proteostasis, and the role played by individual genes and regulatory networks, is essential to develop treatments for these conditions. One potential player is the mitochondrial outer membrane protein Fis1, a crucial fission factor heavily involved in mitochondrial dynamics in yeast but with an unknown role in higher-order organisms. By using Drosophila melanogaster as a model, we explored the effect of Fis1 mutations generated by transposon Minos-mediated integration. Mutants exhibited a higher ratio of damaged mitochondria with age as well as elevated reactive oxygen species levels compared with controls. This caused an increase in oxidative stress, resulting in large accumulations of ubiquitinated proteins, accelerated muscle function decline, and mitochondrial myopathies in young mutant flies. Ectopic expression of Fis1 isoforms was sufficient to suppress this phenotype. Loss of Fis1 led to unbalanced mitochondrial proteostasis within fly muscle, decreasing both flight capabilities and lifespan. Fis1 thus clearly plays a role in fly mitochondrial dynamics. Further investigations into the detailed function of Fis1 are necessary for exploring how mitochondrial function correlates with muscle health during aging.
MeSH term(s)	Aging ; Animals ; Drosophila melanogaster/genetics ; Membrane Proteins/metabolism ; Mitochondrial Proteins/metabolism ; Muscle, Skeletal/metabolism ; Proteostasis/genetics
Chemical Substances	FIS1 protein, human ; Membrane Proteins ; Mitochondrial Proteins
Language	English
Publishing date	2021-06-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2113083-8
ISSN	1474-9726 ; 1474-9718
ISSN (online)	1474-9726
ISSN	1474-9718
DOI	10.1111/acel.13379
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 6581: Show issues

Location:
Je nach Verfügbarkeit (siehe Angabe bei Bestand)
bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
ab Jg. 2022: Lesesaal (EG)

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

Article: And now introducing mammalian mirtrons.

Chan, Shih-Peng / Slack, Frank J

Developmental cell

2007 Volume 13, Issue 5, Page(s) 605–607

Abstract: Mirtrons are short hairpin introns recently found in flies and nematodes that provide an alternative source for animal microRNA biogenesis and use the splicing machinery to bypass Drosha cleavage in initial maturation. The presence of mirtrons outside of ...

Abstract	Mirtrons are short hairpin introns recently found in flies and nematodes that provide an alternative source for animal microRNA biogenesis and use the splicing machinery to bypass Drosha cleavage in initial maturation. The presence of mirtrons outside of invertebrates was not previously known. In the October 26 issue of Molecular Cell, Berezikov et al. expose a number of short mammalian introns as mirtrons.
MeSH term(s)	Animals ; Humans ; Introns ; MicroRNAs/genetics ; MicroRNAs/physiology ; RNA Processing, Post-Transcriptional ; RNA Splicing
Chemical Substances	MicroRNAs
Language	English
Publishing date	2007-07-10
Publishing country	United States
Document type	Journal Article
ZDB-ID	2054967-2
ISSN	1878-1551 ; 1534-5807
ISSN (online)	1878-1551
ISSN	1534-5807
DOI	10.1016/j.devcel.2007.10.010
Database	MEDical Literature Analysis and Retrieval System OnLINE

In stock of ZB MED Cologne/Königswinter

Zs.A 5742: Show issues			Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG)
Zs.MG 76: Show issues

Order via subito

Details ▾
- See ZB MED holdings
- Order with fees

To top

More links

Kategorien

Order via subito

More links

Kategorien

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Bonn / Germany

Order via subito

Inter-library loan at ZB MED

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

More links

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito