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  1. Article ; Online: Surface modification of halide perovskite using EDTA-complexed SnO

    Marques, Nuno / Jana, Santanu / Mendes, Manuel J / Águas, Hugo / Martins, Rodrigo / Panigrahi, Shrabani

    RSC advances

    2024  Volume 14, Issue 18, Page(s) 12397–12406

    Abstract: The long-term performance of metal halide perovskite solar cells (PSCs) can be significantly improved by tuning the surface characteristics of the perovskite layers. Herein, low-temperature-processed ethylenediaminetetraacetic acid (EDTA)-complexed ... ...

    Abstract The long-term performance of metal halide perovskite solar cells (PSCs) can be significantly improved by tuning the surface characteristics of the perovskite layers. Herein, low-temperature-processed ethylenediaminetetraacetic acid (EDTA)-complexed SnO
    Language English
    Publishing date 2024-04-17
    Publishing country England
    Document type Journal Article
    ISSN 2046-2069
    ISSN (online) 2046-2069
    DOI 10.1039/d3ra08900b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Hybrid Digital-Droplet Microfluidic Chip for Applications in Droplet Digital Nucleic Acid Amplification: Design, Fabrication and Characterization.

    Coelho, Beatriz J / Neto, Joana P / Sieira, Bárbara / Moura, André T / Fortunato, Elvira / Martins, Rodrigo / Baptista, Pedro V / Igreja, Rui / Águas, Hugo

    Sensors (Basel, Switzerland)

    2023  Volume 23, Issue 10

    Abstract: Microfluidic-based platforms have become a hallmark for chemical and biological assays, empowering micro- and nano-reaction vessels. The fusion of microfluidic technologies (digital microfluidics, continuous-flow microfluidics, and droplet microfluidics, ...

    Abstract Microfluidic-based platforms have become a hallmark for chemical and biological assays, empowering micro- and nano-reaction vessels. The fusion of microfluidic technologies (digital microfluidics, continuous-flow microfluidics, and droplet microfluidics, just to name a few) presents great potential for overcoming the inherent limitations of each approach, while also elevating their respective strengths. This work exploits the combination of digital microfluidics (DMF) and droplet microfluidics (DrMF) on a single substrate, where DMF enables droplet mixing and further acts as a controlled liquid supplier for a high-throughput nano-liter droplet generator. Droplet generation is performed at a flow-focusing region, operating on dual pressure: negative pressure applied to the aqueous phase and positive pressure applied to the oil phase. We evaluate the droplets produced with our hybrid DMF-DrMF devices in terms of droplet volume, speed, and production frequency and further compare them with standalone DrMF devices. Both types of devices enable customizable droplet production (various volumes and circulation speeds), yet hybrid DMF-DrMF devices yield more controlled droplet production while achieving throughputs that are similar to standalone DrMF devices. These hybrid devices enable the production of up to four droplets per second, which reach a maximum circulation speed close to 1540 µm/s and volumes as low as 0.5 nL.
    MeSH term(s) Microfluidics ; Biological Assay ; Lab-On-A-Chip Devices ; Technology ; Nucleic Acids
    Chemical Substances Nucleic Acids
    Language English
    Publishing date 2023-05-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s23104927
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Isolation of acute myeloid leukemia blasts from blood using a microfluidic device.

    Teixeira, Alexandra / Sousa-Silva, Maria / Chícharo, Alexandre / Oliveira, Kevin / Moura, André / Carneiro, Adriana / Piairo, Paulina / Águas, Hugo / Sampaio-Marques, Belém / Castro, Isabel / Mariz, José / Ludovico, Paula / Abalde-Cela, Sara / Diéguez, Lorena

    The Analyst

    2024  Volume 149, Issue 10, Page(s) 2812–2825

    Abstract: Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults and associated with poor prognosis. Unfortunately, most of the patients that achieve clinical complete remission after the treatment will ultimately relapse due to the ... ...

    Abstract Acute myeloid leukemia (AML) is the most common form of acute leukemia in adults and associated with poor prognosis. Unfortunately, most of the patients that achieve clinical complete remission after the treatment will ultimately relapse due to the persistence of minimal residual disease (MRD), that is not measurable using conventional technologies in the clinic. Microfluidics is a potential tool to improve the diagnosis by providing early detection of MRD. Herein, different designs of microfluidic devices were developed to promote lateral and vertical mixing of cells in microchannels to increase the contact area of the cells of interest with the inner surface of the device. Possible interactions between the cells and the surface were studied using fluid simulations. For the isolation of leukemic blasts, a positive selection strategy was used, targeting the cells of interest using a panel of specific biomarkers expressed in immature and aberrant blasts. Finally, once the optimisation was complete, the best conditions were used to process patient samples for downstream analysis and benchmarking, including phenotypic and genetic characterisation. The potential of these microfluidic devices to isolate and detect AML blasts may be exploited for the monitoring of AML patients at different stages of the disease.
    MeSH term(s) Humans ; Leukemia, Myeloid, Acute/pathology ; Leukemia, Myeloid, Acute/blood ; Cell Separation/methods ; Cell Separation/instrumentation ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques/methods ; Microfluidic Analytical Techniques/instrumentation
    Language English
    Publishing date 2024-05-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d4an00158c
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Copper-Arsenic-Sulfide Thin-Films from Local Raw Materials Deposited via RF Co-Sputtering for Photovoltaics.

    Centeno, Pedro / Alexandre, Miguel / Neves, Filipe / Fortunato, Elvira / Martins, Rodrigo / Águas, Hugo / Mendes, Manuel J

    Nanomaterials (Basel, Switzerland)

    2022  Volume 12, Issue 19

    Abstract: The inexorable increase of energy demand and the efficiency bottleneck of monocrystalline silicon solar cell technology is promoting the research and development of alternative photovoltaic materials. Copper-arsenic-sulfide (CAS) compounds are still ... ...

    Abstract The inexorable increase of energy demand and the efficiency bottleneck of monocrystalline silicon solar cell technology is promoting the research and development of alternative photovoltaic materials. Copper-arsenic-sulfide (CAS) compounds are still rather unexplored in the literature, yet they have been regarded as promising candidates for use as p-type absorber in solar cells, owing to their broad raw material availability, suitable bandgap and high absorption coefficient. Here, a comprehensive study is presented on the structural and optoelectronic properties of CAS thin-films deposited via radio-frequency magnetron co-sputtering, using a commercial Cu target together with a Cu-As-S target with material obtained from local resources, specifically from mines in the Portuguese region of the Iberian Pyrite Belt. Raman and X-ray diffraction analysis confirm that the use of two targets results in films with pronounced stoichiometry gradients, suggesting a transition from amorphous CAS compounds to crystalline djurleite (Cu
    Language English
    Publishing date 2022-09-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano12193268
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: Digital Microfluidics-Powered Real-Time Monitoring of Isothermal DNA Amplification of Cancer Biomarker.

    Coelho, Beatriz Jorge / Veigas, Bruno / Bettencourt, Luís / Águas, Hugo / Fortunato, Elvira / Martins, Rodrigo / Baptista, Pedro V / Igreja, Rui

    Biosensors

    2022  Volume 12, Issue 4

    Abstract: We introduce a digital microfluidics (DMF) platform specifically designed to perform a loop-mediated isothermal amplification (LAMP) of DNA and applied it to a real-time amplification to monitor a cancer biomarker, ...

    Abstract We introduce a digital microfluidics (DMF) platform specifically designed to perform a loop-mediated isothermal amplification (LAMP) of DNA and applied it to a real-time amplification to monitor a cancer biomarker,
    MeSH term(s) Biomarkers, Tumor ; DNA ; Fluorescent Dyes ; Humans ; Microfluidics/methods ; Neoplasms/diagnosis ; Nucleic Acid Amplification Techniques/methods ; Oligonucleotide Array Sequence Analysis
    Chemical Substances Biomarkers, Tumor ; Fluorescent Dyes ; DNA (9007-49-2)
    Language English
    Publishing date 2022-03-28
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2662125-3
    ISSN 2079-6374 ; 2079-6374
    ISSN (online) 2079-6374
    ISSN 2079-6374
    DOI 10.3390/bios12040201
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Transduction Mechanisms, Micro-Structuring Techniques, and Applications of Electronic Skin Pressure Sensors: A Review of Recent Advances.

    Dos Santos, Andreia / Fortunato, Elvira / Martins, Rodrigo / Águas, Hugo / Igreja, Rui

    Sensors (Basel, Switzerland)

    2020  Volume 20, Issue 16

    Abstract: Electronic skin (e-skin), which is an electronic surrogate of human skin, aims to recreate the multifunctionality of skin by using sensing units to detect multiple stimuli, while keeping key features of skin such as low thickness, stretchability, ... ...

    Abstract Electronic skin (e-skin), which is an electronic surrogate of human skin, aims to recreate the multifunctionality of skin by using sensing units to detect multiple stimuli, while keeping key features of skin such as low thickness, stretchability, flexibility, and conformability. One of the most important stimuli to be detected is pressure due to its relevance in a plethora of applications, from health monitoring to functional prosthesis, robotics, and human-machine-interfaces (HMI). The performance of these e-skin pressure sensors is tailored, typically through micro-structuring techniques (such as photolithography, unconventional molds, incorporation of naturally micro-structured materials, laser engraving, amongst others) to achieve high sensitivities (commonly above 1 kPa
    MeSH term(s) Artificial Limbs ; Electronics ; Humans ; Pressure ; Robotics ; Wearable Electronic Devices
    Language English
    Publishing date 2020-08-07
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2052857-7
    ISSN 1424-8220 ; 1424-8220
    ISSN (online) 1424-8220
    ISSN 1424-8220
    DOI 10.3390/s20164407
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Open-source tool for real-time and automated analysis of droplet-based microfluidic.

    Neto, Joana P / Mota, Ana / Lopes, Gonçalo / Coelho, Beatriz J / Frazão, João / Moura, André T / Oliveira, Beatriz / Sieira, Bárbara / Fernandes, José / Fortunato, Elvira / Martins, Rodrigo / Igreja, Rui / Baptista, Pedro V / Águas, Hugo

    Lab on a chip

    2023  Volume 23, Issue 14, Page(s) 3238–3244

    Abstract: Droplet-based microfluidic technology is a powerful tool for generating large numbers of monodispersed nanoliter-sized droplets for ultra-high throughput screening of molecules or single cells. Yet further progress in the development of methods for the ... ...

    Abstract Droplet-based microfluidic technology is a powerful tool for generating large numbers of monodispersed nanoliter-sized droplets for ultra-high throughput screening of molecules or single cells. Yet further progress in the development of methods for the real-time detection and measurement of passing droplets is needed for achieving fully automated systems and ultimately scalability. Existing droplet monitoring technologies are either difficult to implement by non-experts or require complex experimentation setups. Moreover, commercially available monitoring equipment is expensive and therefore limited to a few laboratories worldwide. In this work, we validated for the first time an easy-to-use, open-source Bonsai visual programming language to accurately measure in real-time droplets generated in a microfluidic device. With this method, droplets are found and characterized from bright-field images with high processing speed. We used off-the-shelf components to achieve an optical system that allows sensitive image-based, label-free, and cost-effective monitoring. As a test of its use we present the results, in terms of droplet radius, circulation speed and production frequency, of our method and compared its performance with that of the widely-used ImageJ software. Moreover, we show that similar results are obtained regardless of the degree of expertise. Finally, our goal is to provide a robust, simple to integrate, and user-friendly tool for monitoring droplets, capable of helping researchers to get started in the laboratory immediately, even without programming experience, enabling analysis and reporting of droplet data in real-time and closed-loop experiments.
    Language English
    Publishing date 2023-07-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00327b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: A simple polystyrene microfluidic device for sensitive and accurate SERS-based detection of infection by malaria parasites.

    Oliveira, Maria João / Caetano, Soraia / Dalot, Ana / Sabino, Filipe / Calmeiro, Tomás R / Fortunato, Elvira / Martins, Rodrigo / Pereira, Eulália / Prudêncio, Miguel / Byrne, Hugh J / Franco, Ricardo / Águas, Hugo

    The Analyst

    2023  Volume 148, Issue 17, Page(s) 4053–4063

    Abstract: Early and accurate detection of infection by pathogenic microorganisms, such as Plasmodium, the causative agent of malaria, is critical for clinical diagnosis and ultimately determines the patient's outcome. We have combined a polystyrene-based ... ...

    Abstract Early and accurate detection of infection by pathogenic microorganisms, such as Plasmodium, the causative agent of malaria, is critical for clinical diagnosis and ultimately determines the patient's outcome. We have combined a polystyrene-based microfluidic device with an immunoassay which utilises Surface-Enhanced Raman Spectroscopy (SERS) to detect malaria. The method can be easily translated to a point-of-care testing format and shows excellent sensitivity and specificity, when compared to the gold standard for laboratorial detection of Plasmodium infections. The device can be fabricated in less than 30 min by direct patterning on shrinkable polystyrene sheets of adaptable three-dimensional microfluidic chips. To validate the microfluidic system, samples of
    MeSH term(s) Humans ; Animals ; Parasites ; Polystyrenes ; Plasmodium falciparum ; Reproducibility of Results ; Malaria/diagnosis ; Malaria, Falciparum/diagnosis ; Plasmodium ; Sensitivity and Specificity ; Lab-On-A-Chip Devices
    Chemical Substances Polystyrenes
    Language English
    Publishing date 2023-08-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d3an00971h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article: High UV and Sunlight Photocatalytic Performance of Porous ZnO Nanostructures Synthesized by a Facile and Fast Microwave Hydrothermal Method.

    Ferreira, Sofia Henriques / Morais, Maria / Nunes, Daniela / Oliveira, Maria João / Rovisco, Ana / Pimentel, Ana / Águas, Hugo / Fortunato, Elvira / Martins, Rodrigo

    Materials (Basel, Switzerland)

    2021  Volume 14, Issue 9

    Abstract: The degradation of organic pollutants in wastewaters assisted by oxide semiconductor nanostructures has been the focus of many research groups over the last decades, along with the synthesis of these nanomaterials by simple, eco-friendly, fast, and cost- ... ...

    Abstract The degradation of organic pollutants in wastewaters assisted by oxide semiconductor nanostructures has been the focus of many research groups over the last decades, along with the synthesis of these nanomaterials by simple, eco-friendly, fast, and cost-effective processes. In this work, porous zinc oxide (ZnO) nanostructures were successfully synthesized via a microwave hydrothermal process. A layered zinc hydroxide carbonate (LZHC) precursor was obtained after 15 min of synthesis and submitted to different calcination temperatures to convert it into porous ZnO nanostructures. The influence of the calcination temperature (300, 500, and 700 °C) on the morphological, structural, and optical properties of the ZnO nanostructureswas investigated. All ZnO samples were tested as photocatalysts in the degradation of rhodamine B (RhB) under UV irradiation and natural sunlight. All samples showed enhanced photocatalytic activity under both light sources, with RhB being practically degraded within 60 min in both situations. The porous ZnO obtained at 700 °C showed the greatest photocatalytic activity due to its high crystallinity, with a degradation rate of 0.091 and 0.084 min
    Language English
    Publishing date 2021-05-04
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2487261-1
    ISSN 1996-1944
    ISSN 1996-1944
    DOI 10.3390/ma14092385
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article: Colloidal Lithography for Photovoltaics: An Attractive Route for Light Management.

    Oliveira, Rui D / Mouquinho, Ana / Centeno, Pedro / Alexandre, Miguel / Haque, Sirazul / Martins, Rodrigo / Fortunato, Elvira / Águas, Hugo / Mendes, Manuel J

    Nanomaterials (Basel, Switzerland)

    2021  Volume 11, Issue 7

    Abstract: The pursuit of ever-more efficient, reliable, and affordable solar cells has pushed the development of nano/micro-technological solutions capable of boosting photovoltaic (PV) performance without significantly increasing costs. One of the most relevant ... ...

    Abstract The pursuit of ever-more efficient, reliable, and affordable solar cells has pushed the development of nano/micro-technological solutions capable of boosting photovoltaic (PV) performance without significantly increasing costs. One of the most relevant solutions is based on light management via photonic wavelength-sized structures, as these enable pronounced efficiency improvements by reducing reflection and by trapping the light inside the devices. Furthermore, optimized microstructured coatings allow self-cleaning functionality via effective water repulsion, which reduces the accumulation of dust and particles that cause shading. Nevertheless, when it comes to market deployment, nano/micro-patterning strategies can only find application in the PV industry if their integration does not require high additional costs or delays in high-throughput solar cell manufacturing. As such, colloidal lithography (CL) is considered the preferential structuring method for PV, as it is an inexpensive and highly scalable soft-patterning technique allowing nanoscopic precision over indefinitely large areas. Tuning specific parameters, such as the size of colloids, shape, monodispersity, and final arrangement, CL enables the production of various templates/masks for different purposes and applications. This review intends to compile several recent high-profile works on this subject and how they can influence the future of solar electricity.
    Language English
    Publishing date 2021-06-24
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2662255-5
    ISSN 2079-4991
    ISSN 2079-4991
    DOI 10.3390/nano11071665
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