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  1. Article ; Online: SALBUTAMOL AMELIORATES THE PHENOTYPE OF THE SKIN INFLAMMATORY DISEASE PSORIASIS ACCORDING TO SKIN SPHEROID MODELS

    Berke Demiriz / İrem Türkmen / Berna Aksoy / Özge Sezin Somuncu / Salih Somuncu

    Trakya University Journal of Natural Sciences, Vol 22, Iss 2, Pp 187-

    2021  Volume 197

    Abstract: Psoriasis is a multifactorial chronic inflammatory disorder resulting by the interplay of genetics, the immune system and the environment. It is characterized by the hyperproliferation of epithelial cells, generating red, itchy psoriatic plaques which ... ...

    Abstract Psoriasis is a multifactorial chronic inflammatory disorder resulting by the interplay of genetics, the immune system and the environment. It is characterized by the hyperproliferation of epithelial cells, generating red, itchy psoriatic plaques which have no cure but have great negative impact in patients’ life. Although corticosteroids or vitamin D analogs might help recovery to some extent, there is yet no total cure for the disease. In this study, we sought to generate three-dimensional (3D) stress-related psoriatic skin spheroids with the screening of the potential efficacy of a β2-adrenergic receptor agonist, salbutamol. 3D Culture spheroids with human dermal fibroblasts (HDF), human epithelial keratinocytes (HEK) and human monocytic cell line (THP-1) were generated as a representative model of skin and the protocol of stress-related modelling was conducted. The efficacy of the drug salbutamol was evaluated by the changes in mRNA and protein expression levels of selected genes, as well as by several metabolic assays. We developed a method for culturing spherical organoid models of psoriasis in vitro. We tested the potential theurapetic effects of salbutamol on psoriasis spheroids. Spheroids treated with salbutamol indicated the effictiveness of the treatment. 3D spheroid system was found partially efficient for mimicking the physiological features of psoriasis in vitro. This present work may be a starting point for future investigation as it is the first to generate a stress-related psoriatic model and first to try a β2 agonist as a potential treatment option. Considering the effects and suitability of topical application of salbutamol, its efficacy should not be underestimated and should be investigated further for translating this knowledge into clinics.
    Keywords psoriasis ; skin spheroids ; 3d models ; salbutamol ; Science (General) ; Q1-390
    Subject code 616
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Trakya University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: CONCISE REVIEW

    Umay Çelik / Özge Sezin Somuncu / Büşra Ergün / Emre Arpalı

    Trakya University Journal of Natural Sciences, Vol 20, Pp 45-

    β CELL REPLACEMENT THERAPIES IN TREATMENT OF DIABETES MELLITUS

    2019  Volume 54

    Abstract: Metabolic rate of glucose uptake is generally controlled by a feedback mechanism covering islet β cells and insulin-sensitive tissues, wherein tissue sensitivity to insulin influences the level of β-cell comeback. In case of insulin presence, β cells ... ...

    Abstract Metabolic rate of glucose uptake is generally controlled by a feedback mechanism covering islet β cells and insulin-sensitive tissues, wherein tissue sensitivity to insulin influences the level of β-cell comeback. In case of insulin presence, β cells preserve standard glucose tolerance via enhancing insulin production. Even though β-cell dysfunction has a strong hereditary component, environmental alterations carry an important part as well. Current research methods have facilitated to establish the important part of hexoses, amino acids, and fatty acids in the development of insulin resistance and β-cell dysfunction, therefore more operative treatments to slow the progressive loss of β-cell function are required. Latest discoveries from clinical research deliver significant information about approaches to stop and treat diabetes and some of the adversative properties of these interferences. Generation of satisfactory numbers of pancreatic endocrine cells that work in the same way as primary islets is of supreme prominence for the expansion of cell treatments to cure. In this study, we focused on different techniques starting from islet and pancreas transplantations individually and ending on new therapies such as stem cell technology and bioengineering. We aimed to establish a comprehensive and detailed explanation of treatment perspectives for islet cell loss. This review is carrying a novel potential for enlightening the current treatments and future-based therapies.
    Keywords stem cell therapy ; islet cells ; pancreas ; Science (General) ; Q1-390
    Subject code 571
    Language English
    Publishing date 2019-02-01T00:00:00Z
    Publisher Trakya University
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: Myogenic Differentiation Potential of Human Newborn Foreskin Stem Cells Combined with Polycaprolactone-Based Nanofiber

    Ozge Sezin Somuncu / Ezgi Kasikci / Salih Somuncu / Pakize Neslihan Tasli / Fikrettin Sahin

    Journal of Skin and Stem Cell, Vol 3, Iss

    2016  Volume 1

    Abstract: A previous study performed by the authors of the current study revealed the characterization and differentiation of newly defined stem cells known as human newborn foreskin stem cells (hnFSSCs). According to their stem cell properties, this study aimed ... ...

    Abstract A previous study performed by the authors of the current study revealed the characterization and differentiation of newly defined stem cells known as human newborn foreskin stem cells (hnFSSCs). According to their stem cell properties, this study aimed at investigating myogenic differentiation and related tissue engineering. Human newborn foreskin stem cells were characterized by flow cytometry. The results showed that hnFSSCs carries a noble prospective for myogenic differentiation and can be used as a beneficial method for muscle related diseases, including muscular dystrophy, neuromuscular disorders, muscle damages, muscle weakness, lesion formations, and other problems associated with tissue obtainability and multi-potency; these cells may be accepted as effortlessly accessible and functional, and even superior to other stem cell origins. Furthermore, hnFFSCs were also seeded onto 3D micro-wells and Polycaprolactone (PCL) scaffolds in order to examine tissue development. Human newborn foreskin stem cells on PCL scaffolds showed good cell-cell integration, so that they may be thought as a stem cell basis for tissue engineering.
    Keywords Dermatology ; RL1-803 ; Biology (General) ; QH301-705.5
    Subject code 571 ; 610
    Language English
    Publishing date 2016-03-01T00:00:00Z
    Publisher Skin and Stem Cell Research Center
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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