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Book ; Conference proceedings: Book of abstracts / EMEC 15, Brno 2014, the 15th European Meeting on Environmental Chemistry, Brno, Czech Republic, December 3 - 6, 2014

Čáslavský, Josef

2014

Institution	Vysoké Učení Technické v Brně
Event/congress	EMEC (15, 2014.12.03-06, Brno) ; European Meeting on Environmental Chemistry (15, 2014.12.03-06, Brno)
Author's details	Brno University of Technology ... [Ed.: Josef Čáslavský ...]
Language	English
Size	152 S.
Publishing place	Brno
Document type	Book ; Conference proceedings
ISBN	9788021450738 ; 8021450738
Database	Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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Article ; Online: Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels

Svobodníková, Lucie / Kummerová, Marie / Zezulka, Štěpán / Martinka, Michal / Klemš, Marek / Čáslavský, Josef

Ecotoxicology. 2023 Jan., v. 32, no. 1 p.1-11

2023

Abstract: Pharmaceuticals belong to pseudo-persistent pollutants because of constant entry into the environment and hazardous potential for non-target organisms, including plants, in which they can influence biochemical and physiological processes. Detailed ... ...

Abstract	Pharmaceuticals belong to pseudo-persistent pollutants because of constant entry into the environment and hazardous potential for non-target organisms, including plants, in which they can influence biochemical and physiological processes. Detailed analysis of results obtained by microscopic observations using fluorescent dyes (berberine hemisulphate, Fluorol Yellow 088), detection of phytohormone levels (radioimmunoassay, enzyme-linked immune sorbent assay) and thermogravimetric analysis of lignin content proved that the drug naproxen (NPX) can stimulate the formation of root structural barriers. In the primary root of plants treated with 0.5, 1, and 10 mg/L NPX, earlier Casparian strip formation and development of the whole endodermis circle closer to its apex were found after five days of cultivation (by 9–20% as compared to control) and after ten days from 0.1 mg/L NPX (by 8–63%). Suberin lamellae (SL) were deposited in endodermal cells significantly closer to the apex under 10 mg/L NPX by up to 75%. Structural barrier formation under NPX treatment can be influenced indirectly by auxin-supported cell division and differentiation caused by its eight-times higher level under 10 mg/L NPX and directly by stimulated SL deposition induced by abscisic acid (higher from 0.5 mg/L NPX), as proved by the higher proportion of cells with SL in the primary root base (by 8–44%). The earlier modification of endodermis in plant roots can help to limit the drug transfer and maintain the homeostasis of the plant.
Keywords	abscisic acid ; auxins ; berberine ; cell division ; drugs ; ecotoxicology ; endodermis ; fluorescence ; homeostasis ; lignin content ; peas ; radioimmunoassays ; sorbents ; suberin ; thermogravimetry
Language	English
Dates of publication	2023-01
Size	p. 1-11.
Publishing place	Springer US
Document type	Article ; Online
ZDB-ID	34042-x
ISSN	1573-3017 ; 0963-9292
ISSN (online)	1573-3017
ISSN	0963-9292
DOI	10.1007/s10646-022-02613-8
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Article ; Online: Grain carbon isotopes indicate the ability of wheat plants to maintain enhanced intrinsic water-use efficiency even after short-term exposure to high temperatures and drought.

Pernicová, Natálie / Hlaváčová, Marcela / Findurová, Hana / Čáslavský, Josef / Urban, Otmar / Klem, Karel / Trnka, Miroslav

Plant physiology and biochemistry : PPB

2023 Volume 205, Page(s) 108155

Abstract: Minimizing the impact of heat and drought on crop yields requires varieties with effective protective mechanisms. We tested the hypothesis that even a short-term high temperature amplifies the negative effects of reduced water availability on leaf gas- ... ...

Abstract	Minimizing the impact of heat and drought on crop yields requires varieties with effective protective mechanisms. We tested the hypothesis that even a short-term high temperature amplifies the negative effects of reduced water availability on leaf gas-exchange, but can induce long-lasting improvement in plant water-use efficiency after the stress period. Accordingly, three common varieties of winter wheat (Triticum aestivum) were grown under field conditions. During the stem extension, the plants were exposed to distinct temperatures (daily maximum 26 vs. 38 °C), water availabilities (75% of field water capacity vs. permanent wilting point), and their combination for 14 days. All treatments reduced light-saturated rates of CO
MeSH term(s)	Water/metabolism ; Triticum/metabolism ; Temperature ; Carbon Isotopes ; Droughts ; Edible Grain/metabolism
Chemical Substances	Water (059QF0KO0R) ; Carbon Isotopes
Language	English
Publishing date	2023-11-02
Publishing country	France
Document type	Journal Article
ZDB-ID	742978-2
ISSN	1873-2690 ; 0981-9428
ISSN (online)	1873-2690
ISSN	0981-9428
DOI	10.1016/j.plaphy.2023.108155
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Article ; Online: Impacts of elevated CO

Pernicová, Natálie / Urban, Otmar / Čáslavský, Josef / Kolář, Tomáš / Rybníček, Michal / Sochová, Irena / Peñuelas, Josep / Bošeľa, Michal / Trnka, Miroslav

The Science of the total environment

2024 Volume 921, Page(s) 171173

Abstract: The efficiency of water use in plants, a critical ecophysiological parameter closely related to water and carbon cycles, is essential for understanding the interactions between plants and their environment. This study investigates the effects of ongoing ... ...

Abstract	The efficiency of water use in plants, a critical ecophysiological parameter closely related to water and carbon cycles, is essential for understanding the interactions between plants and their environment. This study investigates the effects of ongoing climate change and increasing atmospheric CO
MeSH term(s)	Ecosystem ; Carbon Dioxide/pharmacology ; Temperature ; Vapor Pressure ; Gases ; Photosynthesis ; Carbon Isotopes/analysis ; Water
Chemical Substances	Carbon Dioxide (142M471B3J) ; Gases ; Carbon Isotopes ; Water (059QF0KO0R)
Language	English
Publishing date	2024-02-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	121506-1
ISSN	1879-1026 ; 0048-9697
ISSN (online)	1879-1026
ISSN	0048-9697
DOI	10.1016/j.scitotenv.2024.171173
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Article ; Online: Pea root responses under naproxen stress: changes in the formation of structural barriers in the primary root in context with changes of auxin and abscisic acid levels.

Svobodníková, Lucie / Kummerová, Marie / Zezulka, Štěpán / Martinka, Michal / Klemš, Marek / Čáslavský, Josef

Ecotoxicology (London, England)

2022 Volume 32, Issue 1, Page(s) 1–11

Abstract	Pharmaceuticals belong to pseudo-persistent pollutants because of constant entry into the environment and hazardous potential for non-target organisms, including plants, in which they can influence biochemical and physiological processes. Detailed analysis of results obtained by microscopic observations using fluorescent dyes (berberine hemisulphate, Fluorol Yellow 088), detection of phytohormone levels (radioimmunoassay, enzyme-linked immune sorbent assay) and thermogravimetric analysis of lignin content proved that the drug naproxen (NPX) can stimulate the formation of root structural barriers. In the primary root of plants treated with 0.5, 1, and 10 mg/L NPX, earlier Casparian strip formation and development of the whole endodermis circle closer to its apex were found after five days of cultivation (by 9-20% as compared to control) and after ten days from 0.1 mg/L NPX (by 8-63%). Suberin lamellae (SL) were deposited in endodermal cells significantly closer to the apex under 10 mg/L NPX by up to 75%. Structural barrier formation under NPX treatment can be influenced indirectly by auxin-supported cell division and differentiation caused by its eight-times higher level under 10 mg/L NPX and directly by stimulated SL deposition induced by abscisic acid (higher from 0.5 mg/L NPX), as proved by the higher proportion of cells with SL in the primary root base (by 8-44%). The earlier modification of endodermis in plant roots can help to limit the drug transfer and maintain the homeostasis of the plant.
MeSH term(s)	Naproxen/toxicity ; Abscisic Acid ; Indoleacetic Acids/analysis ; Pisum sativum ; Plant Roots/chemistry
Chemical Substances	Naproxen (57Y76R9ATQ) ; Abscisic Acid (72S9A8J5GW) ; Indoleacetic Acids
Language	English
Publishing date	2022-12-21
Publishing country	United States
Document type	Journal Article
ZDB-ID	34042-x
ISSN	1573-3017 ; 0963-9292
ISSN (online)	1573-3017
ISSN	0963-9292
DOI	10.1007/s10646-022-02613-8
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Article ; Online: Multi-proxy crossdating extends the longest high-elevation tree-ring chronology from the Mediterranean

Römer, Philipp / Reinig, Frederick / Konter, Oliver / Friedrich, Ronny / Urban, Otmar / Čáslavský, Josef / Pernicová, Natálie / Trnka, Miroslav / Büntgen, Ulf / Esper, Jan

Dendrochronologia. 2023 June, v. 79 p.126085-

2023

Abstract: Annually precise dating is the cornerstone of dendrochronology. The accurate crossdating of relict wood is, however, frequently challenged during early chronology periods when sample replication is typically low. Here we present a multi-proxy approach in ...

Abstract	Annually precise dating is the cornerstone of dendrochronology. The accurate crossdating of relict wood is, however, frequently challenged during early chronology periods when sample replication is typically low. Here we present a multi-proxy approach in which stable carbon (δ¹³C) and radiocarbon (¹⁴C) isotope data are used to evaluate and correct dating errors in the early period of the longest high-elevation tree-ring chronology from the Mediterranean Basin. The record was initially developed using 878 tree-ring width (TRW) and 192 maximum latewood density (MXD) series from living and relict Bosnian pines (Pinus heldreichii) from Mt. Smolikas in Greece to reconstruct hydroclimate and temperature variability back to the 8th century. New annually resolved and non-pooled δ¹³C series now suggest a re-dating of first millennium relict pine samples during a period when sample replication was too low for proper TRW and/or MXD crossdating. The associated correction shifts the start of the Mt. Smolikas chronology from 575 back to 468 CE, a change independently confirmed by wiggle-matching annual ¹⁴C data along the 774/775 CE cosmic event. Our study demonstrates the importance of independent age validation for robust chronology development and shows how multi-proxy crossdating can improve dating success during periods of low sample replication.
Keywords	Pinus heldreichii ; carbon radioisotopes ; dendrochronology ; latewood ; temperature ; wood ; Greece ; Mediterranean region ; Bosnian pine ; Mt. Smolikas ; Paleoclimate ; Radiocarbon isotopes ; Relict wood ; Tree-ring stable isotopes
Language	English
Dates of publication	2023-06
Publishing place	Elsevier GmbH
Document type	Article ; Online
ZDB-ID	2088117-4
ISSN	1612-0051 ; 1125-7865
ISSN (online)	1612-0051
ISSN	1125-7865
DOI	10.1016/j.dendro.2023.126085
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Article: Characteristics of Pyrolysis Oil as Renewable Source of Chemical Materials and Alternative Fuel from the Sewage Sludge Treatment

Chorazy, Tomáš / Čáslavský, Josef / Žvaková, Veronika / Raček, Jakub / Hlavínek, Petr

Waste and biomass valorization. 2020 Aug., v. 11, no. 8

2020

Abstract: This paper describes the present applied research focused on pyrolysis oil as one of the three products of sewage sludge (SS) thermal treatment processing—microwave assisted pyrolysis (MAP). Specifically, the full-scale MAP unit with 3 kW magnetron and ... ...

Abstract	This paper describes the present applied research focused on pyrolysis oil as one of the three products of sewage sludge (SS) thermal treatment processing—microwave assisted pyrolysis (MAP). Specifically, the full-scale MAP unit with 3 kW magnetron and with the frequency of 2.45 GHz at low pressure conditions is in operation at AdMaS Research Centre, Brno University of Technology (AdMaS BUT). The aim of this paper is to present a chemical characterization of obtained pyrolysis oil together with the suitable analytical determination methods. Detailed characterization of pyrolysis oil from SS was realized by precipitation in n-pentane, fractionation of pentane-soluble part by column chromatography on activated silica by stepwise elution and analysis of obtained fractions by comprehensive orthogonal two-dimensional gas chromatography with time-of-flight mass spectrometric detection. Substances from several groups have been identified at the pyrolysis oil from SS sample: alkanes (110 compounds), alkenes and alkynes (184 compounds), cycloalkanes (104 compounds), aromatic and polyaromatic hydrocarbons (646 compounds), further lower numbers of alcohols, aldehydes, esters, carboxylic acids and heterocycles. The paper also presents a comparison of the above mentioned used two chromatographic methods and imply a basic ecotoxicological test—small algal screening.
Keywords	algae ; alkenes ; alkynes ; alternative fuels ; applied research ; biomass ; comprehensive two-dimensional gas chromatography ; ecotoxicology ; fractionation ; heat treatment ; heterocyclic compounds ; mass spectrometry ; pentane ; pyrolysis ; pyrolysis oils ; sewage sludge ; silica
Language	English
Dates of publication	2020-08
Size	p. 4491-4505.
Publishing place	Springer Netherlands
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2541900-6
ISSN	1877-265X ; 1877-2641
ISSN (online)	1877-265X
ISSN	1877-2641
DOI	10.1007/s12649-019-00735-5
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Article ; Online: ERK2 signaling regulates cell-cell adhesion of epithelial cells and enhances growth factor-induced cell scattering.

Rasl, Jan / Grušanović, Josipa / Klímová, Zuzana / Čáslavský, Josef / Groušl, Tomáš / Novotný, Jiří / Kolář, Michal / Vomastek, Tomáš

Cellular signalling

2022 Volume 99, Page(s) 110431

Abstract: The ERK signaling pathway, consisting of core protein kinases Raf, MEK and effector kinases ERK1/2, regulates various biological outcomes such as cell proliferation, differentiation, apoptosis, or cell migration. Signal transduction through the ERK ... ...

Abstract	The ERK signaling pathway, consisting of core protein kinases Raf, MEK and effector kinases ERK1/2, regulates various biological outcomes such as cell proliferation, differentiation, apoptosis, or cell migration. Signal transduction through the ERK signaling pathway is tightly controlled at all levels of the pathway. However, it is not well understood whether ERK pathway signaling can be modulated by the abundance of ERK pathway core kinases. In this study, we investigated the effects of low-level overexpression of the ERK2 isoform on the phenotype and scattering of cuboidal MDCK epithelial cells growing in discrete multicellular clusters. We show that ERK2 overexpression reduced the vertical size of lateral membranes that contain cell-cell adhesion complexes. Consequently, ERK2 overexpressing cells were unable to develop cuboidal shape, remained flat with increased spread area and intercellular adhesive contacts were present only on the basal side. Interestingly, ERK2 overexpression was not sufficient to increase phosphorylation of multiple downstream targets including transcription factors and induce global changes in gene expression, namely to increase the expression of pro-migratory transcription factor Fra1. However, ERK2 overexpression enhanced HGF/SF-induced cell scattering as these cells scattered more rapidly and to a greater extent than parental cells. Our results suggest that an increase in ERK2 expression primarily reduces cell-cell cohesion and that weakened intercellular adhesion synergizes with upstream signaling in the conversion of the multicellular epithelium into single migrating cells. This mechanism may be clinically relevant as the analysis of clinical data revealed that in one type of cancer, pancreatic adenocarcinoma, ERK2 overexpression correlates with a worse prognosis.
MeSH term(s)	Adenocarcinoma/metabolism ; Cell Adhesion ; Cell Proliferation ; Epithelial Cells/metabolism ; Humans ; MAP Kinase Signaling System/physiology ; Mitogen-Activated Protein Kinase 1 ; Mitogen-Activated Protein Kinase Kinases/metabolism ; Pancreatic Neoplasms/metabolism ; Phosphorylation ; Signal Transduction ; Transcription Factors/metabolism
Chemical Substances	Transcription Factors ; MAPK1 protein, human (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase 1 (EC 2.7.11.24) ; Mitogen-Activated Protein Kinase Kinases (EC 2.7.12.2)
Language	English
Publishing date	2022-08-04
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1002702-6
ISSN	1873-3913 ; 0898-6568
ISSN (online)	1873-3913
ISSN	0898-6568
DOI	10.1016/j.cellsig.2022.110431
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Article ; Online: The assembly and function of perinuclear actin cap in migrating cells.

Maninova, Miloslava / Caslavsky, Josef / Vomastek, Tomas

Protoplasma

2017 Volume 254, Issue 3, Page(s) 1207–1218

Abstract: Stress fibers are actin bundles encompassing actin filaments, actin-crosslinking, and actin-associated proteins that represent the major contractile system in the cell. Different types of stress fibers assemble in adherent cells, and they are central to ... ...

Abstract	Stress fibers are actin bundles encompassing actin filaments, actin-crosslinking, and actin-associated proteins that represent the major contractile system in the cell. Different types of stress fibers assemble in adherent cells, and they are central to diverse cellular processes including establishment of the cell shape, morphogenesis, cell polarization, and migration. Stress fibers display specific cellular organization and localization, with ventral fibers present at the basal side, and dorsal fibers and transverse actin arcs rising at the cell front from the ventral to the dorsal side and toward the nucleus. Perinuclear actin cap fibers are a specific subtype of stress fibers that rise from the leading edge above the nucleus and terminate at the cell rear forming a dome-like structure. Perinuclear actin cap fibers are fixed at three points: both ends are anchored in focal adhesions, while the central part is physically attached to the nucleus and nuclear lamina through the linker of nucleoskeleton and cytoskeleton (LINC) complex. Here, we discuss recent work that provides new insights into the mechanism of assembly and the function of these actin stress fibers that directly link extracellular matrix and focal adhesions with the nuclear envelope.
MeSH term(s)	Actin Capping Proteins/metabolism ; Cell Movement/physiology ; Cell Nucleus/metabolism ; Cell Polarity/physiology ; Cell Shape/physiology ; Focal Adhesions/physiology ; Humans ; Mechanotransduction, Cellular/physiology ; Nuclear Envelope/metabolism ; Stress Fibers/physiology
Chemical Substances	Actin Capping Proteins
Language	English
Publishing date	2017-05
Publishing country	Austria
Document type	Journal Article ; Review
ZDB-ID	123809-7
ISSN	1615-6102 ; 0033-183X
ISSN (online)	1615-6102
ISSN	0033-183X
DOI	10.1007/s00709-017-1077-0
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Article: UPLC-MS/MS method for determination of selected pyrrolizidine alkaloids in feed.

Bolechová, Martina / Cáslavský, Josef / Pospíchalová, Markéta / Kosubová, Petra

Food chemistry

2015 Volume 170, Page(s) 265–270

Abstract: Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to ... ...

Abstract	Alkaloids known as secondary metabolites are grouped by typical structural characteristics into large families such as pyrrolizidine alkaloids (PAs) comprising more than 350 individual heterocyclic compounds. The PAs present a serious health risk to human and livestock; hence there is a need for methods that allow these dangerous plant toxins to be determined. In this study, a fast, reliable and sensitive approach is proposed to identify and quantify PAs in feed samples. PAs including monocrotaline, senkirkine, senecionine, seneciphylline and retrorsine were determined by ultra-performance liquid chromatography coupled with tandem mass spectrometry. Sample preparation was based on a modified QuEChERS approach. The mean recovery, precision, matrix effects and limits of quantification were assessed for three matrices within the method validation. The presented method was used to inspect 41 various feed samples, where the presence of PAs was expected. Roughages and feed for rabbits contained the highest levels of PAs, in general.
MeSH term(s)	Chromatography, Liquid/methods ; Pyrrolizidine Alkaloids/chemistry ; Tandem Mass Spectrometry/methods
Chemical Substances	Pyrrolizidine Alkaloids
Language	English
Publishing date	2015-03-01
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	243123-3
ISSN	1873-7072 ; 0308-8146
ISSN (online)	1873-7072
ISSN	0308-8146
DOI	10.1016/j.foodchem.2014.08.072
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