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  1. Article ; Online: A Study on the Stability and Antimicrobial Efficacy of a Newly Modeled Teat Dip Solution Containing Chlorhexidine.

    Kybartas, Modestas / Virgailis, Marius / Ruzauskas, Modestas / Klimienė, Irena / Šiugždinienė, Rita / Merkevičienė, Lina / Štreimikytė-Mockeliūnė, Žaneta / Mockeliūnas, Raimundas

    Veterinary sciences

    2023  Volume 10, Issue 8

    Abstract: Despite much focus on mastitis as an endemic disease, clinical and subclinical mastitis remains an important problem for many herds. Reducing the usage of antibiotics for mastitis treatment allows the risks to be minimized related to the development of ... ...

    Abstract Despite much focus on mastitis as an endemic disease, clinical and subclinical mastitis remains an important problem for many herds. Reducing the usage of antibiotics for mastitis treatment allows the risks to be minimized related to the development of antimicrobial resistance and the excretion of antibiotics into the environment. The aim of the study was to determine the physico-chemical properties, stability and antimicrobial effect of a newly formulated biocide for post-milking udder hygiene containing a thickener made from hydroxypropyl guar gum, an antiseptic chlorhexidine digluconate and teat skin-friendly components including glycerol,
    Language English
    Publishing date 2023-08-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci10080510
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Antimicrobial Activities against Opportunistic Pathogenic Bacteria Using Green Synthesized Silver Nanoparticles in Plant and Lichen Enzyme-Assisted Extracts

    Balčiūnaitienė, Aistė / Štreimikytė, Paulina / Puzerytė, Viktorija / Viškelis, Jonas / Štreimikytė-Mockeliūnė, Žaneta / Maželienė, Žaneta / Sakalauskienė, Vaidė / Viškelis, Pranas

    Plants. 2022 July 13, v. 11, no. 14

    2022  

    Abstract: Enzyme-assisted extraction is a valuable tool for mild and environmentally-friendly extraction conditions to release bioactive compounds and sugars, essential for silver nanoparticle (AgNP) green synthesis as capping and reducing agents. In this research, ...

    Abstract Enzyme-assisted extraction is a valuable tool for mild and environmentally-friendly extraction conditions to release bioactive compounds and sugars, essential for silver nanoparticle (AgNP) green synthesis as capping and reducing agents. In this research, plant and fungal kingdoms were selected to obtain the enzyme-assisted extracts, using green synthesized AgNPs. For the synthesis, pseudo-cereal Fagopyrum esculentum (F. esculentum) and lichen Certaria islandica (C. islandica) extracts were used as environmentally-friendly agents under heating in an aqueous solution. Raw and enzyme-assisted extracts of AgNPs were characterized by physicochemical, phytochemical, and morphological characteristics through scanning and transmission electron microscopy (SEM and TEM), as well as Fourier transform infrared spectroscopy (FTIR). The synthesized nanoparticles were spherical in shape and well dispersed, with average sizes ranging from 10 to 50 nm. This study determined the total phenolic content (TPC) and in vitro antioxidant activity in both materials by applying standard methods. The results showed that TPC, ABTS•⁺, FRAP, and DPPH• radical scavenging activities varied greatly in samples. The AgNPs derived from enzymatic hydrolyzed aqueous extracts C. islandica and F. esculentum exhibited higher antibacterial activity against the tested bacterial pathogens than their respective crude extracts. Results indicate that the extracts’ biomolecules covering the AgNPs may enhance the biological activity of silver nanoparticles and enzyme assistance as a sustainable additive to technological processes to achieve higher yields and necessary media components.
    Keywords Fagopyrum esculentum ; Fourier transform infrared spectroscopy ; antibacterial properties ; antioxidant activity ; aqueous solutions ; enzymes ; fungi ; hydrolysis ; lichens ; nanosilver ; phytochemicals ; pseudocereals ; transmission electron microscopy
    Language English
    Dates of publication 2022-0713
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants11141833
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: Antimicrobial Activities against Opportunistic Pathogenic Bacteria Using Green Synthesized Silver Nanoparticles in Plant and Lichen Enzyme-Assisted Extracts.

    Balčiūnaitienė, Aistė / Štreimikytė, Paulina / Puzerytė, Viktorija / Viškelis, Jonas / Štreimikytė-Mockeliūnė, Žaneta / Maželienė, Žaneta / Sakalauskienė, Vaidė / Viškelis, Pranas

    Plants (Basel, Switzerland)

    2022  Volume 11, Issue 14

    Abstract: Enzyme-assisted extraction is a valuable tool for mild and environmentally-friendly extraction conditions to release bioactive compounds and sugars, essential for silver nanoparticle (AgNP) green synthesis as capping and reducing agents. In this research, ...

    Abstract Enzyme-assisted extraction is a valuable tool for mild and environmentally-friendly extraction conditions to release bioactive compounds and sugars, essential for silver nanoparticle (AgNP) green synthesis as capping and reducing agents. In this research, plant and fungal kingdoms were selected to obtain the enzyme-assisted extracts, using green synthesized AgNPs. For the synthesis, pseudo-cereal
    Language English
    Publishing date 2022-07-13
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2704341-1
    ISSN 2223-7747
    ISSN 2223-7747
    DOI 10.3390/plants11141833
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Hepatitis E genotype 3 virus isolate from wild boar is capable of replication in non-human primate and swine kidney cells and mouse neuroblastoma cells

    Grigas, Juozas / Simkute, Evelina / Simanavicius, Martynas / Pautienius, Arnoldas / Streimikyte-Mockeliune, Zaneta / Razukevicius, Dainius / Stankevicius, Arunas

    BMC veterinary research. 2020 Dec., v. 16, no. 1

    2020  

    Abstract: BACKGROUND: Wild boar-derived hepatitis E (HEV) genotype 3 virus has been successfully isolated in cell lines of human origin only. Considering the zoonotic potential and possible extrahepatic localisation of genotype 3 strain, it is important to ... ...

    Abstract BACKGROUND: Wild boar-derived hepatitis E (HEV) genotype 3 virus has been successfully isolated in cell lines of human origin only. Considering the zoonotic potential and possible extrahepatic localisation of genotype 3 strain, it is important to investigate the viability of cell lines of different animal and tissue origins. Therefore, the objective of the present study was to determine the permissiveness of non-human primate (MARC-145 and Vero) and swine (PK-15) cell lines of kidney origin, and a mouse neuroblastoma (Neuro-2a) cell line for isolation of wild boar-derived HEV genotype 3. RESULTS: This study showed that MARC-145, PK-15, Neuro-2a and Vero cell lines were permissive to wild boar-derived HEV genotype 3 subtype 3i harbouring viral genome equivalents of 1.12 × 107 copies/ml, 2.38 × 105 copies/ml, 2.97 × 107 copies/ml and 4.01 × 107 copies/ml after five serial passages respectively. In all permissive cell lines, HEV was continuously recovered from growth medium between five and at least 28 days post-infection. Peak loads of HEV genome equivalents were observed on days 7, 12, 19 and 30 in MARC-145 (2.88 × 107 copies/ml), Vero (4.23 × 106 copies/ml), Neuro-2a (3.15 × 106 copies/ml) and PK-15 (2.24 × 107 copies/ml) cell lines respectively. In addition, successful virus isolation was confirmed by immunofluorescence assay targeting HEV capsid protein and sequencing of HEV isolate retrieved from cell cultures. CONCLUSIONS: This study showed that wild boar-derived HEV genotype 3 subtype 3i strain was capable of infecting cell lines of animal origin, including primate and porcine kidney cells (MARC-145, PK-15 and Vero), and mouse neuroblastoma cells (Neuro-2a), supporting the notion of the capacity of HEV genotype 3 to cross the species barrier and extra-hepatic localisation of the virus. These findings warrant further studies of tested cell lines to investigate their capacity as an efficient system for HEV propagation. HEV isolates from other wild animal hosts should be isolated on tested cell lines in order to generate more data on HEV transmission between wild animal populations and their role as sources of human infections.
    Keywords cell culture ; cell lines ; cell viability ; coat proteins ; culture media ; fluorescent antibody technique ; genome ; genotype ; hepatitis E ; hosts ; humans ; kidney cells ; kidneys ; mice ; swine ; viruses ; wild boars ; zoonoses
    Language English
    Dates of publication 2020-12
    Size p. 95.
    Publishing place BioMed Central
    Document type Article
    ISSN 1746-6148
    DOI 10.1186/s12917-020-02315-5
    Database NAL-Catalogue (AGRICOLA)

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  5. Article ; Online: Hepatitis E genotype 3 virus isolate from wild boar is capable of replication in non-human primate and swine kidney cells and mouse neuroblastoma cells.

    Grigas, Juozas / Simkute, Evelina / Simanavicius, Martynas / Pautienius, Arnoldas / Streimikyte-Mockeliune, Zaneta / Razukevicius, Dainius / Stankevicius, Arunas

    BMC veterinary research

    2020  Volume 16, Issue 1, Page(s) 95

    Abstract: Background: Wild boar-derived hepatitis E (HEV) genotype 3 virus has been successfully isolated in cell lines of human origin only. Considering the zoonotic potential and possible extrahepatic localisation of genotype 3 strain, it is important to ... ...

    Abstract Background: Wild boar-derived hepatitis E (HEV) genotype 3 virus has been successfully isolated in cell lines of human origin only. Considering the zoonotic potential and possible extrahepatic localisation of genotype 3 strain, it is important to investigate the viability of cell lines of different animal and tissue origins. Therefore, the objective of the present study was to determine the permissiveness of non-human primate (MARC-145 and Vero) and swine (PK-15) cell lines of kidney origin, and a mouse neuroblastoma (Neuro-2a) cell line for isolation of wild boar-derived HEV genotype 3.
    Results: This study showed that MARC-145, PK-15, Neuro-2a and Vero cell lines were permissive to wild boar-derived HEV genotype 3 subtype 3i harbouring viral genome equivalents of 1.12 × 10
    Conclusions: This study showed that wild boar-derived HEV genotype 3 subtype 3i strain was capable of infecting cell lines of animal origin, including primate and porcine kidney cells (MARC-145, PK-15 and Vero), and mouse neuroblastoma cells (Neuro-2a), supporting the notion of the capacity of HEV genotype 3 to cross the species barrier and extra-hepatic localisation of the virus. These findings warrant further studies of tested cell lines to investigate their capacity as an efficient system for HEV propagation. HEV isolates from other wild animal hosts should be isolated on tested cell lines in order to generate more data on HEV transmission between wild animal populations and their role as sources of human infections.
    MeSH term(s) Animals ; Cell Line ; Chlorocebus aethiops ; Genotype ; Hepatitis E/veterinary ; Hepatitis E/virology ; Hepatitis E virus/genetics ; Kidney/cytology ; Mice ; Neuroblastoma/virology ; Phylogeny ; Sus scrofa ; Swine ; Virus Cultivation
    Language English
    Publishing date 2020-03-21
    Publishing country England
    Document type Journal Article
    ISSN 1746-6148
    ISSN (online) 1746-6148
    DOI 10.1186/s12917-020-02315-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

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