LIVIVO - Search results -

Search results

Result 1 - 10 of total 198

Search options

Article ; Online: Production of Astaxanthin Using CBFD1/HFBD1 from Adonis aestivalis and the Isopentenol Utilization Pathway in Escherichia coli

Jared H. Roth / Valerie C. A. Ward

Bioengineering, Vol 10, Iss 1033, p

2023 Volume 1033

Abstract: Astaxanthin is a powerful antioxidant and is used extensively as an animal feed additive and nutraceutical product. Here, we report the use of the β-carotene hydroxylase (CBFD1) and the β-carotene ketolase (HBFD1) from Adonis aestivalis , a flowering ... ...

Abstract	Astaxanthin is a powerful antioxidant and is used extensively as an animal feed additive and nutraceutical product. Here, we report the use of the β-carotene hydroxylase (CBFD1) and the β-carotene ketolase (HBFD1) from Adonis aestivalis , a flowering plant, to produce astaxanthin in E. coli equipped with the P. agglomerans β-carotene pathway and an over-expressed 4-methylerythritol-phosphate (MEP) pathway or the isopentenol utilization pathway (IUP). Introduction of the over-expressed MEP pathway and the IUP resulted in a 3.2-fold higher carotenoid content in LB media at 36 h post-induction compared to the strain containing only the endogenous MEP. However, in M9 minimal media, the IUP pathway dramatically outperformed the over-expressed MEP pathway with an 11-fold increase in total carotenoids produced. The final construct split the large operon into two smaller operons, both with a T7 promoter. This resulted in slightly lower productivity (70.0 ± 8.1 µg/g·h vs. 53.5 ± 3.8 µg/g·h) compared to the original constructs but resulted in the highest proportion of astaxanthin in the extracted carotenoids (73.5 ± 0.2%).
Keywords	carotenoids ; hydrophobic products ; Adonis aestivalis ; Escherichia coli ; isopentenol utilization pathway (IUP) ; Technology ; T ; Biology (General) ; QH301-705.5
Language	English
Publishing date	2023-09-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Airway Management

Sophie A. Saul / Patrick A. Ward / Alistair F. McNarry

Journal of Personalized Medicine, Vol 13, Iss 1327, p

The Current Role of Videolaryngoscopy

2023 Volume 1327

Abstract: Airway management is usually an uncomplicated and safe intervention; however, when problems arise with the primary airway technique, the clinical situation can rapidly deteriorate, resulting in significant patient harm. Videolaryngoscopy has been shown ... ...

Abstract	Airway management is usually an uncomplicated and safe intervention; however, when problems arise with the primary airway technique, the clinical situation can rapidly deteriorate, resulting in significant patient harm. Videolaryngoscopy has been shown to improve patient outcomes when compared with direct laryngoscopy, including improved first-pass success at tracheal intubation, reduced difficult laryngeal views, reduced oxygen desaturation, reduced airway trauma, and improved recognition of oesophageal intubation. The shared view that videolaryngoscopy affords may also facilitate superior teaching, training, and multidisciplinary team performance. As such, its recommended role in airway management has evolved from occasional use as a rescue device (when direct laryngoscopy fails) to a first-intention technique that should be incorporated into routine clinical practice, and this is reflected in recently updated guidelines from a number of international airway societies. However, currently, overall videolaryngoscopy usage is not commensurate with its now widespread availability. A number of factors exist that may be preventing its full adoption, including perceived financial costs, inadequacy of education and training, challenges in achieving deliverable decontamination processes, concerns over sustainability, fears over “de-skilling” at direct laryngoscopy, and perceived limitations of videolaryngoscopes. This article reviews the most up-to-date evidence supporting videolaryngoscopy, explores its current scope of utilisation (including specialist techniques), the potential barriers preventing its full adoption, and areas for future advancement and research.
Keywords	videolaryngoscopy ; videolaryngoscope ; laryngoscopy ; airway management ; Medicine ; R
Language	English
Publishing date	2023-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Literature Review of Safety Event Reporting in Observational Studies

Heather A. Ward / Bao-Anh Nguyen-Khoa / Robert Massouh

Pharmacoepidemiology, Vol 2, Iss 4, Pp 338-

Challenges Extrapolating across Comparable Products

2023 Volume 349

Abstract: Nirmatrelvir/ritonavir (PAXLOVID TM , Pfizer) is an anti-infective inhibiting CYP3A4 indicated for the treatment of COVID-19 in adults at increased risk of severe COVID-19. As a newly approved product, PAXLOVID has limited safety information regarding ... ...

Abstract	Nirmatrelvir/ritonavir (PAXLOVID TM , Pfizer) is an anti-infective inhibiting CYP3A4 indicated for the treatment of COVID-19 in adults at increased risk of severe COVID-19. As a newly approved product, PAXLOVID has limited safety information regarding rare events and serious adverse events (SAEs). This review describes the characterization of the real-world safety profile of products with similar pharmacological properties to PAXLOVID and aims to understand the impact of any drug interaction on the concomitantly prescribed products. A literature search of articles in PubMed published between 2018 and 2023 was conducted to assess the real-world frequency of safety outcomes of interest, specifically those meeting the criteria of serious adverse reaction. The review was restricted to observational, noninterventional studies and included CYP3A4 inhibitors prescribed for short-term treatment of infections in the outpatient setting. Twenty-one articles were included in the review. Most focused on a small, predefined list of safety outcomes and did not provide insight into the broader range of safety outcomes that might occur for the evaluated products with similar pharmacological properties to PAXLOVID or the impact of any interaction on the concomitant product. The findings highlight the challenges in obtaining proxy safety outcomes characteristics via a review of products with comparable pharmacological properties and underscore the need to have large, rapidly accessible data sources that can contribute to the safety profile of newly authorized products in the real world.
Keywords	drug safety ; nirmatrelvir/ritonavir ; PAXLOVID ; real-world data ; serious adverse events ; Therapeutics. Pharmacology ; RM1-950 ; Other systems of medicine ; RZ201-999 ; Public aspects of medicine ; RA1-1270
Subject code	306
Language	English
Publishing date	2023-12-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Adapting Simon’s Two-Stage Design for Efficient Screening of Filovirus Vaccines in Non-Human Primates

Nancy A. Niemuth / Carol L. Sabourin / Lucy A. Ward

Vaccines, Vol 10, Iss 8, p

2022 Volume 1216

Abstract: The cynomolgus monkey ( Macaca fascicularis ) non-human primate (NHP) is widely used for filovirus vaccine testing. To use limited BSL-4 resources efficiently and minimize NHP usage, Simon’s two-stage design was adapted to screen candidate Ebola virus ( ... ...

Abstract	The cynomolgus monkey ( Macaca fascicularis ) non-human primate (NHP) is widely used for filovirus vaccine testing. To use limited BSL-4 resources efficiently and minimize NHP usage, Simon’s two-stage design was adapted to screen candidate Ebola virus (EBOV) vaccines in up to six NHPs with two (optimal), three, or four NHPs in Stage 1. Using the optimal design, two NHPs were tested in Stage 1. If neither survived, the candidate was rejected. Otherwise, it was eligible for Stage 2 testing in four NHPs. Candidates advanced if four or more NHPs were protected over both stages. An 80% efficacious candidate vaccine had 88.5% probability of advancing, and a 40% efficacious candidate vaccine had 83% probability of rejection. Simon’s two-stage design was used to screen 27 EBOV vaccine candidates in 43 candidate regimens that varied in dose, adjuvant, formulation, or schedule. Of the 30 candidate regimens tested using two NHPs in Stage 1, 15 were rejected, nine were withdrawn, and six were tested in Stage 2. All six tested in Stage 2 qualified to advance in the product development pipeline. Multiple regimens for the EBOV vaccines approved by the European Medicines Agency (EMA) and the US Food and Drug Administration (FDA) in 2019 were tested in this program. This approach may also prove useful for screening Sudan virus (SUDV) and Marburg virus (MARV) vaccine candidates.
Keywords	vaccine screening ; Marburg virus (MARV) ; Sudan virus (SUDV) ; Ebola virus (EBOV) ; Simon’s two-stage ; Medicine ; R
Subject code	616
Language	English
Publishing date	2022-07-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Getting Back to the Source, Virtually

Jennifer A. Ward

Arti Musices, Vol 48, Iss 2, Pp 281-

RISM as a Tool in the Digital Environment

2017 Volume 294

Abstract: The online catalogue of the Répertoire International des Sources Musicales (RISM) contains over 1,088,000 records for music manuscripts, imprints, libretti, and treatises. Since the release in 2010, RISM has launched several initiatives to bring ... ...

Abstract	The online catalogue of the Répertoire International des Sources Musicales (RISM) contains over 1,088,000 records for music manuscripts, imprints, libretti, and treatises. Since the release in 2010, RISM has launched several initiatives to bring musicologists closer to the primary source materials they are researching. The online catalogue attempts to expand the database beyond simply recording the locations of musical sources. With the availability of the RISM data as linked open data, RISM is able to collaborate with other projects in the digital humanities and provide data as a basis for research projects. The release of Muscat, RISM’s open- source specialized software for cataloguing musical sources, has made it easier for RISM project participants to catalogue musical sources. This article will describe how the RISM online catalogue brings musicologists closer to primary source materials and how musicologists can work with RISM using Muscat to facilitate and disseminate their own source-based research.
Keywords	musicology ; digital humanities ; musical sources ; databases ; librarianship ; data exchange ; Music ; M1-5000
Subject code	780
Language	English
Publishing date	2017-01-01T00:00:00Z
Publisher	Hrvatsko muzikološko društvo / Croatian Musicological Society
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Cohort profile

Stephanie A Ward / John Maddison / Maria Inacio

BMJ Open, Vol 11, Iss

Dementia in the Registry of Senior Australians

2021 Volume 2

Abstract: Purpose Clinical quality registries (CQRs) are being established in many countries to monitor, benchmark, and report on the quality of dementia care over time. Case ascertainment can be challenging given that diagnosis occurs in a variety of settings. ... ...

Abstract	Purpose Clinical quality registries (CQRs) are being established in many countries to monitor, benchmark, and report on the quality of dementia care over time. Case ascertainment can be challenging given that diagnosis occurs in a variety of settings. The Registry of Senior Australians (ROSA) includes a large cohort of people with dementia from all Australian states and territories identified using routinely collected aged care assessment data. In ROSA, assessment data are linked to information about aged and health service use, medicine dispensing, hospitalisations and the National Death Index. The ROSA dementia cohort was established to capture people for the Australian dementia CQR currently in development who may not be identified elsewhere.Participants There were 373 695 people with dementia identified in aged care assessments from 2008 to 2016. Cross-sectional analysis from the time of cohort entry (e.g. when first identified with dementia on an aged care assessment) indicates that individuals were 84.1 years old on average, and 63.1% were female. More than 44% were first identified at entry to permanent residential aged care. The cohort recorded more severe cognitive impairment at entry than other international dementia registries.Findings to date The cohort has so far been used to demonstrate a declining prevalence of dementia in individuals entering the aged care sector, examine trends in psychotropic medicine prescribing, and to examine the impact of dementia on aged care service use and outcomes.Future plans The ROSA dementia cohort will be updated periodically and is a powerful resource both on its own and as a contributor to the Australian dementia CQR. Integration of the ROSA dementia cohort with the dementia CQR will ensure that people with dementia using aged care services can benefit from the ongoing monitoring and benchmarking of care that a registry can provide.
Keywords	Medicine ; R
Subject code	360
Language	English
Publishing date	2021-02-01T00:00:00Z
Publisher	BMJ Publishing Group
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Synchrony as a measure of conversation difficulty

Lauren V Hadley / Jamie A Ward

PLoS ONE, Vol 16, Iss 10, p e

Movement coherence increases with background noise level and complexity in dyads and triads.

2021 Volume 0258247

Abstract: When people interact, they fall into synchrony. This synchrony has been demonstrated in a range of contexts, from walking or playing music together to holding a conversation, and has been linked to prosocial outcomes such as development of rapport and ... ...

Abstract	When people interact, they fall into synchrony. This synchrony has been demonstrated in a range of contexts, from walking or playing music together to holding a conversation, and has been linked to prosocial outcomes such as development of rapport and efficiency of cooperation. While the basis of synchrony remains unclear, several studies have found synchrony to increase when an interaction is made challenging, potentially providing a means of facilitating interaction. Here we focus on head movement during free conversation. As verbal information is obscured when conversing over background noise, we investigate whether synchrony is greater in high vs low levels of noise, as well as addressing the effect of background noise complexity. Participants held a series of conversations with unfamiliar interlocutors while seated in a lab, and the background noise level changed every 15-30s between 54, 60, 66, 72, and 78 dB. We report measures of head movement synchrony recorded via high-resolution motion tracking at the extreme noise levels (i.e., 54 vs 78 dB) in dyads (n = 15) and triads (n = 11). In both the dyads and the triads, we report increased movement coherence in high compared to low level speech-shaped noise. Furthermore, in triads we compare behaviour in speech-shaped noise vs multi-talker babble, and find greater movement coherence in the more complex babble condition. Key synchrony differences fall in the 0.2-0.5 Hz frequency bands, and are discussed in terms of their correspondence to talkers' average utterance durations. Additional synchrony differences occur at higher frequencies in the triads only (i.e., >5 Hz), which may relate to synchrony of backchannel cues (as multiple individuals were listening and responding to the same talker). Not only do these studies replicate prior work indicating interlocutors' increased reliance on behavioural synchrony as task difficulty increases, but they demonstrate these effects using multiple difficulty manipulations and across different sized interaction groups.
Keywords	Medicine ; R ; Science ; Q
Subject code	150
Language	English
Publishing date	2021-01-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Book: Current approaches to occupational health / 3

Gardner, A. Ward

1987

Author's details	ed. by A. Ward Gardner
Collection	Current approaches to occupational health
Size	XX, 354 S. : graph. Darst.
Publisher	Wright
Publishing place	Bristol u.a.
Publishing country	Great Britain
Document type	Book
HBZ-ID	HT002943378
ISBN	0-7236-0739-7 ; 978-0-7236-0739-7
Database	Catalogue ZB MED Medicine, Health

In stock of ZB MED Cologne/Königswinter

Shelf mark	Loan status	Location
Se 964 -3-	verfügbar in Königswinter	Königswinter

Order via subito

This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

Details ▾
- See ZB MED holdings
- Order with fees

Article ; Online: XPA

Lucia Borszéková Pulzová / Thomas A. Ward / Miroslav Chovanec

International Journal of Molecular Sciences, Vol 21, Iss 6, p

DNA Repair Protein of Significant Clinical Importance

2020 Volume 2182

Abstract: The nucleotide excision repair (NER) pathway is activated in response to a broad spectrum of DNA lesions, including bulky lesions induced by platinum-based chemotherapeutic agents. Expression levels of NER factors and resistance to chemotherapy has been ... ...

Abstract	The nucleotide excision repair (NER) pathway is activated in response to a broad spectrum of DNA lesions, including bulky lesions induced by platinum-based chemotherapeutic agents. Expression levels of NER factors and resistance to chemotherapy has been examined with some suggestion that NER plays a role in tumour resistance; however, there is a great degree of variability in these studies. Nevertheless, recent clinical studies have suggested Xeroderma Pigmentosum group A (XPA) protein, a key regulator of the NER pathway that is essential for the repair of DNA damage induced by platinum-based chemotherapeutics, as a potential prognostic and predictive biomarker for response to treatment. XPA functions in damage verification step in NER, as well as a molecular scaffold to assemble other NER core factors around the DNA damage site, mediated by protein−protein interactions. In this review, we focus on the interacting partners and mechanisms of regulation of the XPA protein. We summarize clinical oncology data related to this DNA repair factor, particularly its relationship with treatment outcome, and examine the potential of XPA as a target for small molecule inhibitors.
Keywords	xpa protein ; nucleotide excision repair ; biomarker ; cancer ; small molecule inhibitors ; single nucleotide polymorphism ; prognostic and predictive value ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	500
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Role of Complement and Histones in Sepsis

Firas S. Zetoune / Peter A. Ward

Frontiers in Medicine, Vol

2020 Volume 7

Abstract: The wide use of the mouse model of polymicrobial sepsis has provided important evidence for events occurring in infectious sepsis involving septic mice and septic humans. Nearly 100 clinical trials in humans with sepsis have been completed, yet there is ... ...

Abstract	The wide use of the mouse model of polymicrobial sepsis has provided important evidence for events occurring in infectious sepsis involving septic mice and septic humans. Nearly 100 clinical trials in humans with sepsis have been completed, yet there is no FDA-approved drug. Our studies of polymicrobial sepsis have highlighted the role of complement activation products (especially C5a anaphylatoxin and its receptors C5aR1 and C5aR2) in adverse effects of sepsis. During sepsis, the appearance of these complement products is followed by appearance of extracellular histones in plasma, which have powerful proinflammatory and prothrombotic activities that cause cell injury and multiorgan dysfunction in septic mice. Similar responses occur in septic humans. Histone appearance in plasma is related to complement activation and appearance of C5a and its interaction with its receptors. Development of the cardiomyopathy of sepsis also depends on C5a, C5a receptors and histones. Neutralization of C5a with antibody or absence of C5aR1 blocks appearance of extracellular histones and cell and organ failure in sepsis. Survival rates in septic mice are greatly improved after blockade of C5a with antibody. We also review the various strategies in sepsis that greatly reduce the development of life-threatening events of sepsis.
Keywords	anaphylatoxins ; histones ; NLRP3 inflammasome ; ROS ; NETs ; METs ; Medicine (General) ; R5-920
Language	English
Publishing date	2020-12-01T00:00:00Z
Publisher	Frontiers Media S.A.
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Kategorien

In stock of ZB MED Cologne/Königswinter

Order via subito

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED