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  1. Article ; Online: Prevalence and Antibiogram of Escherichia coli isolates recovered from bovine milk

    Abdelrahman Subhi / Aalaa Saad / Kamelia Osman / Mahmoud Hashad / Heba Deif

    Journal of Applied Veterinary Sciences, Vol 8, Iss 3, Pp 82-

    2023  Volume 90

    Abstract: Antimicrobial resistance is considered a major threat facing humanity. It doesn’t only affect public health, but also causes great losses in the dairy industry. Mastitis is a major threat to the dairy industry. The aim of this study was to monitor the ... ...

    Abstract Antimicrobial resistance is considered a major threat facing humanity. It doesn’t only affect public health, but also causes great losses in the dairy industry. Mastitis is a major threat to the dairy industry. The aim of this study was to monitor the antimicrobial resistance of Escherichia coli (E. coli) collected from raw milk of both healthy and mastitis cows and buffaloes in Egypt. In total, 450 milk samples were collected and examined in the period from 2018 to 2021. The samples were collected from healthy cows and buffaloes (30, 58), suffering from clinical mastitis (139, 223) respectively. E. coli was isolated from 33 mastitis milk samples (9.1%) and from 3 (3.4%) normal milk samples. The antibiotic susceptibility testing was performed using the disc diffusion method (Kirby-Bauer method). E. coli isolated from mastitis milk samples showed resistance to amoxicillin-clavulanic acid (75.8%), ceftazidime (75.8%), colistin (69.8%), ampicillin (39.4%), imipenem (24.2%), trimethoprim-sulfamethoxazole (24.2%), aztreonam (15.2%), cefotaxime (15.2%), tetracycline (15.2%), ciprofloxacin (12.2%), chloramphenicol (6%), and gentamicin (3%). E. coli isolated from normal milk samples showed resistance to ampicillin, and imipenem (66.7%), each, and amoxicillin-clavulanic acid, tetracycline, and trimethoprim-sulfamethoxazole (33.3%), each. The Extended Spectrum -Lactamases test (ESBL) performed on the E. coli isolates showed positive results in 9% of mastitis milk samples, but no results in normal milk samples. Out of 36 E. coli isolates, 34 possessed the ampC gene, but blaTEM and blaSHV were detected in 5 isolates with percentages of 94%, 1.4%, and 1.4%, respectively, while BlaIPM and Sul1 were found in one isolate (2.7%), for each.
    Keywords antibiogram ; bovine mastitis ; e. coli ; esbls ; milk ; Zoology ; QL1-991 ; Veterinary medicine ; SF600-1100 ; Animal biochemistry ; QP501-801
    Subject code 630
    Language English
    Publishing date 2023-07-01T00:00:00Z
    Publisher Egyptian Society for Animal Management
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Microbiological, Phenotypic Characteristics, Antimicrobial Resistance Profile and Molecular Identification of Acinetobacter species Isolated from Meat of Different Sources in Egypt

    Aya A.A. Ahmed / Heba N. Deif / Aalaa Saad / Kamelia M. Osman

    Journal of Applied Veterinary Sciences, Vol 6, Iss 4, Pp 67-

    2021  Volume 74

    Abstract: Although food is very important for human life, it may be life-threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens include many types of bacterial species. ... ...

    Abstract Although food is very important for human life, it may be life-threatening. Foodborne diseases are spreading worldwide through the increasing rate of fresh and undercooked food consumption. Foodborne pathogens include many types of bacterial species. This study was conducted to determine the prevalence of Acinetobacter species isolated from meat samples and their phenotypic characteristics, antimicrobial resistance profiles, and genotypic characteristics. A total of 110 samples, collected from chickens (n=50), beef (n=44), rabbits (n=10), and mutton (n=6), were examined bacteriologically. The suspected colonies were identified biochemically and then tested for antimicrobial resistance, biofilm formation, and hemolytic activity; identification was confirmed by Polymerase Chain Reaction (PCR) for the genes; rpoB, tarT, fimH, and espA. Nine Acinetobacter species isolates (8.2%) were recovered. Fifty five samples resulted in the isolation of non-lactose fermenters with an incidence of 50%, 29 produced late lactose fermenters with an incidence of 26%. The rest of the samples showed no growth or non-lactose fermenters. On antibiogram, the isolates showed high resistance to ceftriaxone, imipenem, ceftazidime and ticarcillin/clavulanic acid in percentages of 89%, 77.8%, 66.7% and 66.7 %, respectively. While low resistance was found to sulfamethazole/ trimethoprim, doxycycline and amikacin in percentages of 44.4%, 33.3% and 11.1%, respectively. However, the isolates showed no resistance to ciprofloxacin. All the isolates were MDR with MDRindex (more than 0.5). Only one isolate was a weak biofilm producer but, no isolates produced hemolysis of the sheep RBCs. Genetically, 88.9% of the isolates expressed tarT and fimH genes, while only 5.6% of the isolates expressed espA gene. It can be concluded that Acinetobacter species are to be considered when inspecting meat samples of different sources.
    Keywords acinetobacter ; ast ; meat ; pcr ; virulence ; Zoology ; QL1-991 ; Veterinary medicine ; SF600-1100 ; Animal biochemistry ; QP501-801
    Subject code 580
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher Egyptian Society for Animal Management
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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