LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 5 of total 5

Search options

  1. Article ; Online: Profiling of repetitive RNA sequences in the blood plasma of patients with cancer.

    Reggiardo, Roman E / Maroli, Sreelakshmi Velandi / Peddu, Vikas / Davidson, Andrew E / Hill, Alexander / LaMontagne, Erin / Aaraj, Yassmin Al / Jain, Miten / Chan, Stephen Y / Kim, Daniel H

    Nature biomedical engineering

    2023  Volume 7, Issue 12, Page(s) 1627–1635

    Abstract: Liquid biopsies provide a means for the profiling of cell-free RNAs secreted by cells throughout the body. Although well-annotated coding and non-coding transcripts in blood are readily detectable and can serve as biomarkers of disease, the overall ... ...

    Abstract Liquid biopsies provide a means for the profiling of cell-free RNAs secreted by cells throughout the body. Although well-annotated coding and non-coding transcripts in blood are readily detectable and can serve as biomarkers of disease, the overall diagnostic utility of the cell-free transcriptome remains unclear. Here we show that RNAs derived from transposable elements and other repeat elements are enriched in the cell-free transcriptome of patients with cancer, and that they serve as signatures for the accurate classification of the disease. We used repeat-element-aware liquid-biopsy technology and single-molecule nanopore sequencing to profile the cell-free transcriptome in plasma from patients with cancer and to examine millions of genomic features comprising all annotated genes and repeat elements throughout the genome. By aggregating individual repeat elements to the subfamily level, we found that samples with pancreatic cancer are enriched with specific Alu subfamilies, whereas other cancers have their own characteristic cell-free RNA profile. Our findings show that repetitive RNA sequences are abundant in blood and can be used as disease-specific diagnostic biomarkers.
    MeSH term(s) Humans ; RNA/genetics ; Base Sequence ; DNA Transposable Elements ; Plasma ; Neoplasms/diagnosis ; Neoplasms/genetics ; Biomarkers
    Chemical Substances RNA (63231-63-0) ; DNA Transposable Elements ; Biomarkers
    Language English
    Publishing date 2023-08-31
    Publishing country England
    Document type Journal Article
    ISSN 2157-846X
    ISSN (online) 2157-846X
    DOI 10.1038/s41551-023-01081-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Genetic regulation and targeted reversal of lysosomal dysfunction and inflammatory sterol metabolism in pulmonary arterial hypertension.

    Harvey, Lloyd D / Alotaibi, Mona / Kim, Hee-Jung Janice / Tai, Yi-Yin / Tang, Ying / Sun, Wei / El Khoury, Wadih / Woodcock, Chen-Shan C / Aaraj, Yassmin Al / St Croix, Claudette M / Stolz, Donna B / Lee, Jiyoung / Cheng, Mary Hongying / Schwantes-An, Tae-Hwi / Desai, Ankit A / Pauciulo, Michael W / Nichols, William C / Webb, Amy / Lafyatis, Robert /
    Nouraie, Mehdi / Wu, Haodi / McDonald, Jeffrey G / Chauvet, Caroline / Cheng, Susan / Bahar, Ivet / Bertero, Thomas / Benza, Raymond L / Jain, Mohit / Chan, Stephen Y

    bioRxiv : the preprint server for biology

    2024  

    Abstract: Vascular inflammation critically regulates endothelial cell (EC) pathophenotypes, particularly in pulmonary arterial hypertension (PAH). Dysregulation of lysosomal activity and cholesterol metabolism have known inflammatory roles in disease, but their ... ...

    Abstract Vascular inflammation critically regulates endothelial cell (EC) pathophenotypes, particularly in pulmonary arterial hypertension (PAH). Dysregulation of lysosomal activity and cholesterol metabolism have known inflammatory roles in disease, but their relevance to PAH is unclear. In human pulmonary arterial ECs and in PAH, we found that inflammatory cytokine induction of the nuclear receptor coactivator 7 (NCOA7) both preserved lysosomal acidification and served as a homeostatic brake to constrain EC immunoactivation. Conversely, NCOA7 deficiency promoted lysosomal dysfunction and proinflammatory oxysterol/bile acid generation that, in turn, contributed to EC pathophenotypes. In vivo, mice deficient for Ncoa7 or exposed to the inflammatory bile acid 7α-hydroxy-3-oxo-4-cholestenoic acid (7HOCA) displayed worsened PAH. Emphasizing this mechanism in human PAH, an unbiased, metabolome-wide association study (N=2,756) identified a plasma signature of the same NCOA7-dependent oxysterols/bile acids associated with PAH mortality (P<1.1x10-6). Supporting a genetic predisposition to NCOA7 deficiency, in genome-edited, stem cell-derived ECs, the common variant intronic SNP rs11154337 in NCOA7 regulated NCOA7 expression, lysosomal activity, oxysterol/bile acid production, and EC immunoactivation. Correspondingly, SNP rs11154337 was associated with PAH severity via six-minute walk distance and mortality in discovery (N=93, P=0.0250; HR=0.44, 95% CI [0.21-0.90]) and validation (N=630, P=2x10-4; HR=0.49, 95% CI [0.34-0.71]) cohorts. Finally, utilizing computational modeling of small molecule binding to NCOA7, we predicted and synthesized a novel activator of NCOA7 that prevented EC immunoactivation and reversed indices of rodent PAH. In summary, we have established a genetic and metabolic paradigm and a novel therapeutic agent that links lysosomal biology as well as oxysterol and bile acid processes to EC inflammation and PAH pathobiology. This paradigm carries broad implications for diagnostic and therapeutic development in PAH and in other conditions dependent upon acquired and innate immune regulation of vascular disease.
    Language English
    Publishing date 2024-03-01
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.02.26.582142
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Long-term Outcomes for Teen Mothers Who Participated in a Mentoring Program to Prevent Repeat Teen Pregnancy.

    Lin, Chyongchiou Jeng / Nowalk, Mary Patricia / Ncube, Collette N / Aaraj, Yassmin Al / Warshel, McKenzie / South-Paul, Jeannette E

    Journal of the National Medical Association

    2018  Volume 111, Issue 3, Page(s) 296–301

    Abstract: Background: Each year in the United States (US), one million adolescents are pregnant, of which approximately 20% are repeat pregnancies. Adolescent motherhood is associated with lower educational attainment, socioeconomic status and poorer health ... ...

    Abstract Background: Each year in the United States (US), one million adolescents are pregnant, of which approximately 20% are repeat pregnancies. Adolescent motherhood is associated with lower educational attainment, socioeconomic status and poorer health outcomes. A mentoring program called the Maikuru Program conducted from 2011 to 2015, was designed to teach young mothers under 20 years old how to face daily life challenges, to support them by pairing them with an adult mentor, and prevent a subsequent pregnancy during their teens. The goal of the present study was to examine educational attainment, employment and pregnancies of these adolescent mothers 1-5 years post program.
    Methods: Former participants of the Maikuru Program were contacted by telephone and/or Facebook in 2016 to conduct a survey about education attainment, employment status, number of subsequent children delivered, and satisfaction with the program.
    Results: Nineteen of 51 participants (37%) were reached to complete the survey. Of those who responded, all were in high school or had graduated, nearly half were pursuing some form of higher education and 12 (63%) were currently employed. Nine mothers had given birth to another child; only two (10.5%) were known to be less than 20 years old at the time. All participants reported positive perceptions of the program and would recommend it to other adolescent mothers.
    Conclusion: Educational achievement and employment were high among a modest proportion of adolescent mothers who had participated in a culturally tailored, teen mother-adult mentoring program. Repeat teen pregnancy was infrequent and the mentoring program was perceived as contributing to the success of those who responded to the follow-up. A future randomized trial based on this model may confirm these findings.
    MeSH term(s) Adolescent ; African Americans/psychology ; African Americans/statistics & numerical data ; Age Factors ; Educational Status ; Employment/statistics & numerical data ; Female ; Humans ; Interviews as Topic ; Mentoring/methods ; Pennsylvania ; Pregnancy ; Pregnancy in Adolescence/prevention & control ; Program Evaluation ; Young Adult
    Language English
    Publishing date 2018-11-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 419737-9
    ISSN 1943-4693 ; 0027-9684
    ISSN (online) 1943-4693
    ISSN 0027-9684
    DOI 10.1016/j.jnma.2018.10.014
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.B 150: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Awareness and Use of Contraceptive Methods and Perceptions of Long-Acting Reversible Contraception Among White and Non-White Women.

    Lin, Chyongchiou J / Maier, John / Nwankwo, Chidinma / Burley, Cassie / deBorja, Leyan / Aaraj, Yassmin Al / Lewis, Elizabeth / Rhem, Marla / Nowalk, Mary Patricia / South-Paul, Jeannette

    Journal of women's health (2002)

    2020  Volume 30, Issue 9, Page(s) 1313–1320

    Abstract: Background: ...

    Abstract Background:
    MeSH term(s) Female ; Humans ; Long-Acting Reversible Contraception ; Perception
    Language English
    Publishing date 2020-12-09
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1139774-3
    ISSN 1931-843X ; 1059-7115 ; 1540-9996
    ISSN (online) 1931-843X
    ISSN 1059-7115 ; 1540-9996
    DOI 10.1089/jwh.2020.8642
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 3584: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Frataxin deficiency promotes endothelial senescence in pulmonary hypertension.

    Culley, Miranda K / Zhao, Jingsi / Tai, Yi Yin / Tang, Ying / Perk, Dror / Negi, Vinny / Yu, Qiujun / Woodcock, Chen-Shan C / Handen, Adam / Speyer, Gil / Kim, Seungchan / Lai, Yen-Chun / Satoh, Taijyu / Watson, Annie Mm / Aaraj, Yassmin Al / Sembrat, John / Rojas, Mauricio / Goncharov, Dmitry / Goncharova, Elena A /
    Khan, Omar F / Anderson, Daniel G / Dahlman, James E / Gurkar, Aditi U / Lafyatis, Robert / Fayyaz, Ahmed U / Redfield, Margaret M / Gladwin, Mark T / Rabinovitch, Marlene / Gu, Mingxia / Bertero, Thomas / Chan, Stephen Y

    The Journal of clinical investigation

    2021  Volume 131, Issue 11

    Abstract: The dynamic regulation of endothelial pathophenotypes in pulmonary hypertension (PH) remains undefined. Cellular senescence is linked to PH with intracardiac shunts; however, its regulation across PH subtypes is unknown. Since endothelial deficiency of ... ...

    Abstract The dynamic regulation of endothelial pathophenotypes in pulmonary hypertension (PH) remains undefined. Cellular senescence is linked to PH with intracardiac shunts; however, its regulation across PH subtypes is unknown. Since endothelial deficiency of iron-sulfur (Fe-S) clusters is pathogenic in PH, we hypothesized that a Fe-S biogenesis protein, frataxin (FXN), controls endothelial senescence. An endothelial subpopulation in rodent and patient lungs across PH subtypes exhibited reduced FXN and elevated senescence. In vitro, hypoxic and inflammatory FXN deficiency abrogated activity of endothelial Fe-S-containing polymerases, promoting replication stress, DNA damage response, and senescence. This was also observed in stem cell-derived endothelial cells from Friedreich's ataxia (FRDA), a genetic disease of FXN deficiency, ataxia, and cardiomyopathy, often with PH. In vivo, FXN deficiency-dependent senescence drove vessel inflammation, remodeling, and PH, whereas pharmacologic removal of senescent cells in Fxn-deficient rodents ameliorated PH. These data offer a model of endothelial biology in PH, where FXN deficiency generates a senescent endothelial subpopulation, promoting vascular inflammatory and proliferative signals in other cells to drive disease. These findings also establish an endothelial etiology for PH in FRDA and left heart disease and support therapeutic development of senolytic drugs, reversing effects of Fe-S deficiency across PH subtypes.
    MeSH term(s) Animals ; Cellular Senescence/genetics ; Endothelial Progenitor Cells/metabolism ; Endothelial Progenitor Cells/pathology ; Endothelium, Vascular/metabolism ; Endothelium, Vascular/pathology ; Female ; Friedreich Ataxia/genetics ; Friedreich Ataxia/metabolism ; Friedreich Ataxia/pathology ; Humans ; Hypertension, Pulmonary/genetics ; Hypertension, Pulmonary/metabolism ; Hypertension, Pulmonary/pathology ; Iron-Binding Proteins/genetics ; Iron-Binding Proteins/metabolism ; Male ; Mice ; Mice, Knockout ; Vascular Remodeling/genetics ; Frataxin
    Chemical Substances Iron-Binding Proteins
    Language English
    Publishing date 2021-04-27
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Video-Audio Media
    ZDB-ID 3067-3
    ISSN 1558-8238 ; 0021-9738
    ISSN (online) 1558-8238
    ISSN 0021-9738
    DOI 10.1172/JCI136459
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.184: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top