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  1. AU="Abaci, Irem"
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  3. AU="Mendez, Luis"
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  5. AU="Heidi G Standke"
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  1. Artikel ; Online: Investigation of the Antiviral Effects of Saussurea lappa Root Extract against SARS‐CoV‐2 Virus

    Ulbegi Polat, Hivda / Serhatli, Müge / Taş Ekiz, Arzu / Abaci, İrem / Aksoy, Özge / Çakirca, Gamze / Tekin, Şaban / Al‐Zendani, Abdulmajeed Aziz Hamoud / Al‐Zendani, Mohammed Abdulmajeed Aziz / Korkmaz, Ömer / Peru, Celalettin

    Molecular Nutrition & Food Research. 2023 July, v. 67, no. 14 p.e2200804-

    2023  

    Abstract: SCOPE: The purpose of this study was to look into the antiviral activity of a plant extract derived from the roots of the Saussurea lappa as a food supplement against SARS‐CoV‐2 infection. METHODS AND RESULTS: Vero E6 cells are employed in the study to ... ...

    Abstract SCOPE: The purpose of this study was to look into the antiviral activity of a plant extract derived from the roots of the Saussurea lappa as a food supplement against SARS‐CoV‐2 infection. METHODS AND RESULTS: Vero E6 cells are employed in the study to test the neutralizing effect of Saussurea lappa extract against the SARS‐CoV‐2 virus. For anti‐viral activity detection, a sensitive real‐time cell analyzer (xCELLigence RTCA) with a high repetition rate is used. A challenge experiment in mice is planned as a result of the in vitro analysis. A challenge test against SARS‐CoV‐2 is performed with 10 adult female K18‐hACE2 transgenic mice in each group for this purpose. The mice in the S. lappa Group are gavaged 2 days before the virus is administered intranasally (i.n.). The control group received PBS instead of the extract. SARS‐CoV‐2 virus is administered i.n. under anesthesia for the first 3 days of the experiment, and S. lappa extract was administered by gavage in the afternoon. On the 10th day, mice in the S. lappa group survived the study, whereas animals in the control group grew ill and/or died. In this study, the extract protects the mice against the SARS‐CoV‐2 virus in 90% of the cases. CONCLUSIONS: This study demonstrates that the Saussurea plant has antiviral effects against SARS‐CoV‐2 in vitro and in animal models.
    Schlagwörter Saussurea costus ; Severe acute respiratory syndrome coronavirus 2 ; adults ; anesthesia ; animals ; antiviral properties ; dietary supplements ; females ; food research ; genetically modified organisms ; root extracts ; viruses
    Sprache Englisch
    Erscheinungsverlauf 2023-07
    Erscheinungsort John Wiley & Sons, Ltd
    Dokumenttyp Artikel ; Online
    Anmerkung JOURNAL ARTICLE
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202200804
    Datenquelle NAL Katalog (AGRICOLA)

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  2. Artikel ; Online: Therapeutic Effect of C-Vx Substance in K18-hACE2 Transgenic Mice Infected with SARS-CoV-2.

    Ulbegi Polat, Hivda / Abaci, Irem / Tas Ekiz, Arzu / Aksoy, Ozge / Oktelik, Fatma Betul / Yilmaz, Vuslat / Tekin, Saban / Okyar, Alper / Oncul, Oral / Deniz, Gunnur

    International journal of molecular sciences

    2023  Band 24, Heft 15

    Abstract: C-Vx is a bioprotective product designed to boost the immune system. This study aimed to determine the antiviral activity of the C-Vx substance against SARS-CoV-2 infection. The effect of C-Vx in K18-hACE2 transgenic mice against the SARS-CoV-2 virus was ...

    Abstract C-Vx is a bioprotective product designed to boost the immune system. This study aimed to determine the antiviral activity of the C-Vx substance against SARS-CoV-2 infection. The effect of C-Vx in K18-hACE2 transgenic mice against the SARS-CoV-2 virus was investigated. For this purpose, ten mice were separated into experimental and control groups. Animals were infected with SARS-CoV-2 prior to the administration of the product to determine whether the product has a therapeutic effect similar to that demonstrated in previous human studies, at a histopathological and molecular level. C-Vx-treated mice survived the challenge, whereas the control mice became ill and/or died. The cytokine-chemokine panel with blood samples taken during the critical days of the disease revealed detailed immune responses. Our findings showed that C-Vx presented 90% protection against the SARS-CoV-2 virus-infected mice. The challenge results and cytokine responses of K18-hACE2 transgenic mice matched previous scientific studies, demonstrating the C-Vx's antiviral efficiency.
    Mesh-Begriff(e) Humans ; Mice ; Animals ; Mice, Transgenic ; SARS-CoV-2 ; COVID-19 ; Antiviral Agents/pharmacology ; Antiviral Agents/therapeutic use ; Cytokines ; Disease Models, Animal
    Chemische Substanzen K-18 conjugate ; Antiviral Agents ; Cytokines
    Sprache Englisch
    Erscheinungsdatum 2023-07-26
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2019364-6
    ISSN 1422-0067 ; 1422-0067 ; 1661-6596
    ISSN (online) 1422-0067
    ISSN 1422-0067 ; 1661-6596
    DOI 10.3390/ijms241511957
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Investigation of the Antiviral Effects of Saussurea lappa Root Extract against SARS-CoV-2 Virus.

    Ulbegi Polat, Hivda / Serhatli, Müge / Taş Ekiz, Arzu / Abaci, İrem / Aksoy, Özge / Çakirca, Gamze / Tekin, Şaban / Al-Zendani, Abdulmajeed Aziz Hamoud / Al-Zendani, Mohammed Abdulmajeed Aziz / Korkmaz, Ömer / Peru, Celalettin

    Molecular nutrition & food research

    2023  Band 67, Heft 14, Seite(n) e2200804

    Abstract: Scope: The purpose of this study was to look into the antiviral activity of a plant extract derived from the roots of the Saussurea lappa as a food supplement against SARS-CoV-2 infection.: Methods and results: Vero E6 cells are employed in the study ...

    Abstract Scope: The purpose of this study was to look into the antiviral activity of a plant extract derived from the roots of the Saussurea lappa as a food supplement against SARS-CoV-2 infection.
    Methods and results: Vero E6 cells are employed in the study to test the neutralizing effect of Saussurea lappa extract against the SARS-CoV-2 virus. For anti-viral activity detection, a sensitive real-time cell analyzer (xCELLigence RTCA) with a high repetition rate is used. A challenge experiment in mice is planned as a result of the in vitro analysis. A challenge test against SARS-CoV-2 is performed with 10 adult female K18-hACE2 transgenic mice in each group for this purpose. The mice in the S. lappa Group are gavaged 2 days before the virus is administered intranasally (i.n.). The control group received PBS instead of the extract. SARS-CoV-2 virus is administered i.n. under anesthesia for the first 3 days of the experiment, and S. lappa extract was administered by gavage in the afternoon. On the 10th day, mice in the S. lappa group survived the study, whereas animals in the control group grew ill and/or died. In this study, the extract protects the mice against the SARS-CoV-2 virus in 90% of the cases.
    Conclusions: This study demonstrates that the Saussurea plant has antiviral effects against SARS-CoV-2 in vitro and in animal models.
    Mesh-Begriff(e) Mice ; Animals ; Saussurea ; SARS-CoV-2 ; Antiviral Agents/pharmacology ; COVID-19 ; Plant Extracts/pharmacology
    Chemische Substanzen Antiviral Agents ; Plant Extracts
    Sprache Englisch
    Erscheinungsdatum 2023-05-25
    Erscheinungsland Germany
    Dokumenttyp Journal Article
    ZDB-ID 2160372-8
    ISSN 1613-4133 ; 1613-4125
    ISSN (online) 1613-4133
    ISSN 1613-4125
    DOI 10.1002/mnfr.202200804
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Alternatively spliced MEFV transcript lacking exon 2 and its protein isoform pyrin-2d implies an epigenetic regulation of the gene in inflammatory cell culture models.

    Erdem, Gokce Celikyapi / Erdemir, Sule / Abaci, Irem / Aydin, Asli K Kirectepe / Everest, Elif / Turanli, Eda Tahir

    Genetics and molecular biology

    2017  Band 40, Heft 3, Seite(n) 688–697

    Abstract: The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the ...

    Abstract The function of gene body DNA methylation in alternative splicing, and its relation to disease pathogenesis is not fully elucidated. The gene for familial Mediterranean fever (MEFV) encodes the pyrin protein and contains a 998 bp CpG island, covering the second exon, which is differentially methylated in FMF patients compared to healthy controls. Our further observation of increased exon 2-spliced MEFV transcript in leukocytes of FMF patients provoked us to test the role of exon methylation in alternative splicing using inflammatory cell culture models. First, in vitro exon methylation triggered an increased level of exon 2 exclusion using a splicing cassette in a promyelocytic leukemia cell line (HL-60). HL-60 cells subjected to methylating and demethylating agents, as well as cells differentiated to neutrophil-like cells, exhibited different levels of spliced/unspliced transcripts. We observed increased levels of spliced transcripts in neutrophil-like (p = 0.0005), activated (p = 0.0034) and methylated cells (p < 0.0001), whereas decreased levels in demethylated cells (p = 0.0126) compared to control untreated HL-60 cells. We also showed that the protein isoform of pyrin lacking the exon 2 has an adverse subcellular localization in neutrophil-like cells. Therefore, it remains in the cytoplasm rather than the nucleus. This may point to an epigenetic involvement in an important inflammatory gene.
    Sprache Englisch
    Erscheinungsdatum 2017-08-31
    Erscheinungsland Brazil
    Dokumenttyp Journal Article
    ZDB-ID 1445712-x
    ISSN 1678-4685 ; 1415-4757
    ISSN (online) 1678-4685
    ISSN 1415-4757
    DOI 10.1590/1678-4685-GMB-2016-0234
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Process development for an effective COVID-19 vaccine candidate harboring recombinant SARS-CoV-2 delta plus receptor binding domain produced by Pichia pastoris.

    Kalyoncu, Sibel / Yilmaz, Semiramis / Kuyucu, Ayca Zeybek / Sayili, Dogu / Mert, Olcay / Soyturk, Hakan / Gullu, Seyda / Akinturk, Huseyin / Citak, Erhan / Arslan, Merve / Taskinarda, Melda Guray / Tarman, Ibrahim Oguzhan / Altun, Gizem Yilmazer / Ozer, Ceren / Orkut, Ridvan / Demirtas, Aysegul / Tilmensagir, Idil / Keles, Umur / Ulker, Ceren /
    Aralan, Gizem / Mercan, Yavuz / Ozkan, Muge / Caglar, Hasan Onur / Arik, Gizem / Ucar, Mehmet Can / Yildirim, Muzaffer / Yildirim, Tugce Canavar / Karadag, Dilara / Bal, Erhan / Erdogan, Aybike / Senturk, Serif / Uzar, Serdar / Enul, Hakan / Adiay, Cumhur / Sarac, Fahriye / Ekiz, Arzu Tas / Abaci, Irem / Aksoy, Ozge / Polat, Hivda Ulbegi / Tekin, Saban / Dimitrov, Stefan / Ozkul, Aykut / Wingender, Gerhard / Gursel, Ihsan / Ozturk, Mehmet / Inan, Mehmet

    Scientific reports

    2023  Band 13, Heft 1, Seite(n) 5224

    Abstract: Recombinant protein-based SARS-CoV-2 vaccines are needed to fill the vaccine equity gap. Because protein-subunit based vaccines are easier and cheaper to produce and do not require special storage/transportation conditions, they are suitable for low-/ ... ...

    Abstract Recombinant protein-based SARS-CoV-2 vaccines are needed to fill the vaccine equity gap. Because protein-subunit based vaccines are easier and cheaper to produce and do not require special storage/transportation conditions, they are suitable for low-/middle-income countries. Here, we report our vaccine development studies with the receptor binding domain of the SARS-CoV-2 Delta Plus strain (RBD-DP) which caused increased hospitalizations compared to other variants. First, we expressed RBD-DP in the Pichia pastoris yeast system and upscaled it to a 5-L fermenter for production. After three-step purification, we obtained RBD-DP with > 95% purity from a protein yield of > 1 g/L of supernatant. Several biophysical and biochemical characterizations were performed to confirm its identity, stability, and functionality. Then, it was formulated in different contents with Alum and CpG for mice immunization. After three doses of immunization, IgG titers from sera reached to > 10
    Mesh-Begriff(e) Animals ; Humans ; Mice ; COVID-19 Vaccines ; SARS-CoV-2/genetics ; COVID-19/prevention & control ; Mice, Transgenic ; Saccharomyces cerevisiae ; Antibodies, Viral ; Antibodies, Neutralizing
    Chemische Substanzen COVID-19 Vaccines ; Antibodies, Viral ; Antibodies, Neutralizing
    Sprache Englisch
    Erscheinungsdatum 2023-03-30
    Erscheinungsland England
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-023-32021-9
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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