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  1. Article ; Online: Modular barcode beads for microfluidic single cell genomics.

    Delley, Cyrille L / Abate, Adam R

    Scientific reports

    2021  Volume 11, Issue 1, Page(s) 10857

    Abstract: Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is ... ...

    Abstract Barcode beads allow efficient nucleic acid tagging in single cell genomics. Current barcode designs, however, are fabricated with a particular application in mind. Repurposing to novel targets, or altering to add additional targets as information is obtained is possible but the result is suboptimal. Here, we describe a modular framework that simplifies generation of multifunctional beads and allows their easy extension to new targets.
    MeSH term(s) Biomarkers, Tumor ; DNA Barcoding, Taxonomic ; Gene Expression Profiling ; Gene Expression Regulation, Neoplastic ; Genomics/methods ; Humans ; Microfluidics/methods ; Neoplasms/etiology ; Single-Cell Analysis/methods ; Transcriptome
    Chemical Substances Biomarkers, Tumor
    Language English
    Publishing date 2021-05-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-021-90255-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Flow cytometric printing of double emulsions into open droplet arrays.

    Zhang, Pengfei / Xu, Linfeng / Chen, Huawei / Abate, Adam R

    Lab on a chip

    2023  Volume 23, Issue 10, Page(s) 2371–2377

    Abstract: Delivery of double emulsions in air is crucial for their applications in mass spectrometry, bioanalytics, and material synthesis. However, while methods have been developed to generate double emulsions in air, controlled printing of double emulsion ... ...

    Abstract Delivery of double emulsions in air is crucial for their applications in mass spectrometry, bioanalytics, and material synthesis. However, while methods have been developed to generate double emulsions in air, controlled printing of double emulsion droplets has not been achieved yet. In this paper, we present an approach for in-air printing of double emulsions on demand. Our approach pre-encapsulates reagents in an emulsion that is reinjected into the device, and generates double emulsions in a microfluidic printhead with spatially patterned wettability. Our device allows sorting of ejected double emulsion droplets in real-time, allowing deterministic printing of each droplet to be selected with the desired inner cores. Our method provides a general platform for building printed double emulsion droplet arrays of defined composition at scale.
    Language English
    Publishing date 2023-05-16
    Publishing country England
    Document type Journal Article
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d3lc00151b
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Microfluidic particle zipper enables controlled loading of droplets with distinct particle types.

    Delley, Cyrille L / Abate, Adam R

    Lab on a chip

    2020  Volume 20, Issue 14, Page(s) 2465–2472

    Abstract: Current encapsulation approaches control the number of particles encapsulated per droplet, but not the particle types; consequently, they are unable to generate droplets containing combinations of distinct particle types, limiting the reactions that can ... ...

    Abstract Current encapsulation approaches control the number of particles encapsulated per droplet, but not the particle types; consequently, they are unable to generate droplets containing combinations of distinct particle types, limiting the reactions that can be performed. We describe a microfluidic particle zipper that allows the number and types of particles encapsulated in every droplet to be controlled. The approach exploits self-ordering to generate repeating particle patterns that allow controlled encapsulation in droplets. We use the method to combine barcode particles with gel encapsulated cells to profile multiple disease relevant genomic loci with single cell sequencing. Particle zippers can operate in series to generate complex particle compositions in droplets.
    MeSH term(s) Microfluidic Analytical Techniques ; Microfluidics
    Language English
    Publishing date 2020-06-25
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d0lc00339e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: High-Definition Single-Cell Printing: Cell-by-Cell Fabrication of Biological Structures.

    Zhang, Pengfei / Abate, Adam R

    Advanced materials (Deerfield Beach, Fla.)

    2020  Volume 32, Issue 52, Page(s) e2005346

    Abstract: Bioprinting is a powerful technology with the potential to transform medical device manufacturing, organ replacement, and the treatment of diseases and physiologic malformations. However, current bioprinters are unable to reliably print the fundamental ... ...

    Abstract Bioprinting is a powerful technology with the potential to transform medical device manufacturing, organ replacement, and the treatment of diseases and physiologic malformations. However, current bioprinters are unable to reliably print the fundamental unit of all living things, single cells. A high-definition single-cell printing, a novel microfluidic technology, is presented here that can accurately print single cells from a mixture of multiple candidates. The bioprinter employs a highly miniaturized microfluidic sorter to deterministically select single cells of interest for printing, achieving an accuracy of ≈10 µm and speed of ≈100 Hz. This approach is demonstrated by fabricating intricate cell patterns with pre-defined features through selective single-cell printing. The approach is used to synthesize well-defined spheroids with controlled composition and morphology. The speed, accuracy, and flexibility of the approach will advance bioprinting to enable new studies in organoid science, tissue engineering, and spatially targeted cell therapies.
    MeSH term(s) Bioprinting/methods ; Cell Line, Tumor ; Humans ; Lab-On-A-Chip Devices ; Organoids/metabolism
    Language English
    Publishing date 2020-11-18
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1474949-X
    ISSN 1521-4095 ; 0935-9648
    ISSN (online) 1521-4095
    ISSN 0935-9648
    DOI 10.1002/adma.202005346
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Sequencing Ultrarare Targets with Compound Nucleic Acid Cytometry

    Sun, Chen / Chang, Kai-Chun / Abate, Adam R

    Analytical chemistry. 2021 May 10, v. 93, no. 20

    2021  

    Abstract: Targeted sequencing enables sensitive and cost-effective analysis by focusing resources on molecules of interest. Existing methods, however, are limited in enrichment power and target capture length. Here, we present a novel method that uses compound ... ...

    Abstract Targeted sequencing enables sensitive and cost-effective analysis by focusing resources on molecules of interest. Existing methods, however, are limited in enrichment power and target capture length. Here, we present a novel method that uses compound nucleic acid cytometry to achieve million-fold enrichments of molecules >10 kbp in length using minimal prior target information. We demonstrate the approach by sequencing HIV proviruses in infected individuals. Our method is useful for rare target sequencing in research and clinical applications, including for identifying cancer-associated mutations or sequencing viruses infecting cells.
    Keywords analytical chemistry ; cost effectiveness ; nucleic acids ; proviruses
    Language English
    Dates of publication 2021-0510
    Size p. 7422-7429.
    Publishing place American Chemical Society
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.0c04749
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 4' 52/94: Show issues
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  6. Article ; Online: Controlled fabrication of functional liver spheroids with microfluidic flow cytometric printing.

    Zhang, Pengfei / Li, Xiangpeng / Chen, Jennifer Y / Abate, Adam R

    Biofabrication

    2022  Volume 14, Issue 4

    Abstract: Multicellular liver spheroids are 3D culture models useful in the development of therapies for liver fibrosis. While these models can recapitulate fibrotic disease, current methods for generating them via random aggregation are uncontrolled, yielding ... ...

    Abstract Multicellular liver spheroids are 3D culture models useful in the development of therapies for liver fibrosis. While these models can recapitulate fibrotic disease, current methods for generating them via random aggregation are uncontrolled, yielding spheroids of variable size, function, and utility. Here, we report fabrication of precision liver spheroids with microfluidic flow cytometric printing. Our approach fabricates spheroids cell-by-cell, yielding structures with exact numbers of different cell types. Because spheroid function depends on composition, our precision spheroids have superior functional uniformity, allowing more accurate and statistically significant screens compared to randomly generated spheroids. The approach produces thousands of spheroids per hour, and thus affords a scalable platform by which to manufacture single-cell precision spheroids for disease modeling and high throughput drug testing.
    MeSH term(s) Cell Culture Techniques/methods ; Cell Survival ; Liver ; Microfluidics/methods ; Printing, Three-Dimensional ; Spheroids, Cellular
    Language English
    Publishing date 2022-08-23
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2500944-8
    ISSN 1758-5090 ; 1758-5082
    ISSN (online) 1758-5090
    ISSN 1758-5082
    DOI 10.1088/1758-5090/ac8622
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Robotic automation of droplet microfluidics.

    Tran, Tuan M / Kim, Samuel C / Modavi, Cyrus / Abate, Adam R

    Biomicrofluidics

    2022  Volume 16, Issue 1, Page(s) 14102

    Abstract: Droplet microfluidics enables powerful analytic capabilities but often requires workflows involving macro- and microfluidic processing steps that are cumbersome to perform manually. Here, we demonstrate the automation of droplet microfluidics with ... ...

    Abstract Droplet microfluidics enables powerful analytic capabilities but often requires workflows involving macro- and microfluidic processing steps that are cumbersome to perform manually. Here, we demonstrate the automation of droplet microfluidics with commercial fluid-handling robotics. The workflows incorporate common microfluidic devices including droplet generators, mergers, and sorters and utilize the robot's native capabilities for thermal control, incubation, and plate scanning. The ability to automate microfluidic devices using commercial fluid handling will speed up the integration of these methods into biological workflows.
    Language English
    Publishing date 2022-02-03
    Publishing country United States
    Document type Journal Article
    ISSN 1932-1058
    ISSN 1932-1058
    DOI 10.1063/5.0064265
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Polyhedral Particles with Controlled Concavity by Indentation Templating.

    Weisgerber, Daniel W / Hatori, Makiko / Li, Xiangpeng / Abate, Adam R

    Analytical chemistry

    2022  Volume 94, Issue 21, Page(s) 7475–7482

    Abstract: Current methods for fabricating microparticles offer limited control over size and shape. Here, we demonstrate a droplet microfluidic method to form polyhedral microparticles with controlled concavity. By manipulating Laplace pressure, buoyancy, and ... ...

    Abstract Current methods for fabricating microparticles offer limited control over size and shape. Here, we demonstrate a droplet microfluidic method to form polyhedral microparticles with controlled concavity. By manipulating Laplace pressure, buoyancy, and particle rheology, we generate microparticles with diverse shapes and curvatures. Additionally, we demonstrate the particles provide increased capture efficiency when used for particle-templated emulsification. Our approach enables microparticles with enhanced chemical and biological functionality.
    MeSH term(s) Microfluidic Analytical Techniques/methods ; Microfluidics ; Particle Size ; Rheology
    Language English
    Publishing date 2022-05-16
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, Non-U.S. Gov't ; Research Support, N.I.H., Extramural
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.1c04884
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  9. Article ; Online: Particle Templated Emulsification enables Microfluidic-Free Droplet Assays.

    Weisgerber, Daniel W / Hatori, Makiko N / Abate, Adam R

    Journal of visualized experiments : JoVE

    2021  , Issue 169

    Abstract: Reactions performed in monodispersed droplets afford enhanced accuracy and sensitivity compared to equivalent ones performed in bulk. However, the requirement of microfluidics to form controlled droplets imposes a barrier to non-experts, limiting their ... ...

    Abstract Reactions performed in monodispersed droplets afford enhanced accuracy and sensitivity compared to equivalent ones performed in bulk. However, the requirement of microfluidics to form controlled droplets imposes a barrier to non-experts, limiting their use. Here, we describe particle templated emulsification, an approach to generate monodisperse droplets without microfluidics. Using templating hydrogel spheres, we encapsulate samples in monodispersed droplets by simple vortexing. We demonstrate the approach by using it to perform microfluidic-free digital PCR.
    MeSH term(s) Biological Assay/methods ; Microfluidic Analytical Techniques/methods
    Language English
    Publishing date 2021-03-09
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S. ; Video-Audio Media
    ZDB-ID 2259946-0
    ISSN 1940-087X ; 1940-087X
    ISSN (online) 1940-087X
    ISSN 1940-087X
    DOI 10.3791/62248
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Precision ejection of microfluidic droplets into air with a superhydrophobic outlet.

    Zhang, Pengfei / Chang, Kai-Chun / Abate, Adam R

    Lab on a chip

    2021  Volume 21, Issue 8, Page(s) 1484–1491

    Abstract: Dispensing micron-scale droplets from a suspended nozzle is important for applications in bioprinting, analytical chemistry, and pharmaceutical formulation. Here, we describe a general approach to eject droplets from microfluidic devices using ... ...

    Abstract Dispensing micron-scale droplets from a suspended nozzle is important for applications in bioprinting, analytical chemistry, and pharmaceutical formulation. Here, we describe a general approach to eject droplets from microfluidic devices using superhydrophobic patterning; this facilitates release of wetted fluids, allowing droplets to break contact with channel surfaces and travel along regular paths to achieve a printing accuracy of ∼3 μm. We demonstrate the utility of the approach by using it to print droplets of varied composition from a microfluidic mixing device. Our approach is compatible with common fabrication techniques making it applicable to devices configured for diverse applications.
    MeSH term(s) Hydrophobic and Hydrophilic Interactions ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques ; Microfluidics
    Language English
    Publishing date 2021-03-19
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S.
    ZDB-ID 2056646-3
    ISSN 1473-0189 ; 1473-0197
    ISSN (online) 1473-0189
    ISSN 1473-0197
    DOI 10.1039/d0lc01327g
    Database MEDical Literature Analysis and Retrieval System OnLINE

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