LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Discriminatory power of a circulating multi-noncoding RNA panel in acute coronary syndrome subtypes: Towards precision detection.

    Agwa, Sara H A / Elzahwy, Sherif Samir / Hossam, Nourhan / Yahia, Yahia A / Hamady, Shaimaa / Sherif, Nadine / Elshazly, Ahmed / Darwish, Reham M / Hashim, Jomana Osama / Adly, Mahmoud Ashraf / Abd Elsamee, Aya M / Shamekh, Rania / Roushdy, Marian Maher Salib / Matboli, Marwa

    The international journal of biochemistry & cell biology

    2024  Volume 169, Page(s) 106531

    Abstract: Background: Acute Coronary Syndrome (ACS) stands as a significant contributor to cardiovascular mortality, necessitating improved diagnostic tools for early detection and tailored therapeutic interventions. Current diagnostic modalities, exhibit ... ...

    Abstract Background: Acute Coronary Syndrome (ACS) stands as a significant contributor to cardiovascular mortality, necessitating improved diagnostic tools for early detection and tailored therapeutic interventions. Current diagnostic modalities, exhibit limitations in sensitivity and specificity, urging the quest for novel biomarkers to enhance discrimination of the different stages of ACS including unstable angina, Non-ST-segment Elevation Myocardial Infarction (NSTEMI), and ST-segment Elevation Myocardial Infarction (STEMI).
    Methods: This study investigated the potential of a plasma-circulating multi-noncoding RNA (ncRNA) panel, comprising four miRNAs (miR-182-5p, miR-23a-3p, miR-146a-5p, and miR-183-5p) and three lncRNAs (SNHG15, SNHG5, and RMRP), selected based on their intricate involvement in ACS pathogenesis and signaling pathways regulating post-myocardial infarction (MI) processes. The differential expression of these ncRNAs was validated in sera of ACS patients and healthy controls via real-time polymerase chain reaction (RT-PCR).
    Results: Analysis revealed a marked upregulation of the multi-ncRNAs panel in ACS patients. Notably, miRNA-182-5p and lncRNA-RMRP exhibited exceptional discriminatory power, indicated by the high area under the curve (AUC) values (0.990 and 0.980, respectively). Importantly, this panel displayed superior efficacy in discriminating between STEMI and NSTEMI, outperforming conventional biomarkers like creatine kinase-MB and cardiac troponins. Additionally, the four miRNAs and lncRNA RMRP showcased remarkable proficiency in distinguishing between STEMI and unstable angina.
    Conclusion: The findings underscore the promising potential of the multi-ncRNA panel as a robust tool for early ACS detection, and precise differentiation among ACS subtypes, and as a potential therapeutic target.
    MeSH term(s) Humans ; Acute Coronary Syndrome/diagnosis ; Acute Coronary Syndrome/genetics ; ST Elevation Myocardial Infarction/diagnosis ; ST Elevation Myocardial Infarction/therapy ; Non-ST Elevated Myocardial Infarction/diagnosis ; Non-ST Elevated Myocardial Infarction/pathology ; RNA, Long Noncoding/genetics ; MicroRNAs/genetics ; Myocardial Infarction/diagnosis ; Myocardial Infarction/genetics ; Biomarkers ; Angina, Unstable/diagnosis ; Angina, Unstable/genetics
    Chemical Substances RNA, Long Noncoding ; MicroRNAs ; Biomarkers ; Mirn182 microRNA, human
    Language English
    Publishing date 2024-01-26
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 1228429-4
    ISSN 1878-5875 ; 1357-2725
    ISSN (online) 1878-5875
    ISSN 1357-2725
    DOI 10.1016/j.biocel.2024.106531
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 431: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  2. Article: Identifying SARS-CoV-2 Lineage Mutation Hallmarks and Correlating Them With Clinical Outcomes in Egypt: A Pilot Study.

    Agwa, Sara H A / Elghazaly, Hesham / El Meteini, Mahmoud Shawky / Yahia, Yahia A / Khaled, Radwa / Abd Elsamee, Aya M / Darwish, Reham M / Elsayed, Shaimaa M / Hafez, Hala / Mahmoud, Basma S / Em, Fouda / Matboli, Marwa

    Frontiers in molecular biosciences

    2022  Volume 9, Page(s) 817735

    Abstract: The SARS-CoV-2 pandemic has led to over 4.9 million deaths as of October 2021. One of the main challenges of creating vaccines, treatment, or diagnostic tools for the virus is its mutations and emerging variants. A couple of variants were declared as ... ...

    Abstract The SARS-CoV-2 pandemic has led to over 4.9 million deaths as of October 2021. One of the main challenges of creating vaccines, treatment, or diagnostic tools for the virus is its mutations and emerging variants. A couple of variants were declared as more virulent and infectious than others. Some approaches were used as nomenclature for SARS-CoV-2 variants and lineages. One of the most used is the Pangolin nomenclature. In our study, we enrolled 35 confirmed SARS-CoV-2 patients and sequenced the viral RNA in their samples. We also aimed to highlight the hallmark mutations in the most frequent lineage. We identified a seven-mutation signature for the SARS-CoV-2
    Language English
    Publishing date 2022-03-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2814330-9
    ISSN 2296-889X
    ISSN 2296-889X
    DOI 10.3389/fmolb.2022.817735
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: Association between

    Agwa, Sara H A / Kamel, Marwa Mostafa / Elghazaly, Hesham / Abd Elsamee, Aya M / Hafez, Hala / Girgis, Samia Abdo / Ezz Elarab, Hoda / Ebeid, Fatma S E / Sayed, Safa Matbouly / Sherif, Lina / Matboli, Marwa

    Genes

    2021  Volume 12, Issue 6

    Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection provides a critical host-immunological challenge.: Aim: We explore the effect of host-genetic variation in interferon-lambda-3 rs12979860, Tolloid Like-1 (: Methods!# ...

    Abstract Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection provides a critical host-immunological challenge.
    Aim: We explore the effect of host-genetic variation in interferon-lambda-3 rs12979860, Tolloid Like-1 (
    Methods: 141 COVID-19 positive patients and 100 healthy controls were tested for interferon-lambda-3 rs12979860,
    Results: There were statistically significant differences between the studied cases and control group with regard to the presence of comorbidities, total leucocytic count, lymphocytic count, CRP, serum LDH, ferritin and D-dimer (
    Conclusion: Among people who carry C and A alleles of SNPs
    MeSH term(s) Alleles ; Biomarkers ; COVID-19/diagnosis ; COVID-19/genetics ; COVID-19/immunology ; COVID-19/virology ; Case-Control Studies ; Comorbidity ; Cytokines/metabolism ; Discoidin Domain Receptor 1/genetics ; Female ; Genetic Predisposition to Disease ; Genotype ; Host-Pathogen Interactions/genetics ; Host-Pathogen Interactions/immunology ; Humans ; Immunity, Innate ; Interferons/genetics ; Male ; Polymorphism, Single Nucleotide ; Prognosis ; SARS-CoV-2/physiology ; Severity of Illness Index ; Tolloid-Like Metalloproteinases/genetics ; Viral Load
    Chemical Substances Biomarkers ; Cytokines ; interferon-lambda, human ; Interferons (9008-11-1) ; DDR1 protein, human (EC 2.7.10.1) ; Discoidin Domain Receptor 1 (EC 2.7.10.1) ; Tolloid-Like Metalloproteinases (EC 3.4.-) ; TLL1 protein, human (EC 3.4.24.-)
    Language English
    Publishing date 2021-05-28
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12060830
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: In Silico Identification and Clinical Validation of a Novel Long Non-Coding RNA/mRNA/miRNA Molecular Network for Potential Biomarkers for Discriminating SARS CoV-2 Infection Severity.

    Agwa, Sara H A / Elghazaly, Hesham / Meteini, Mahmoud Shawky El / Shawky, Sherif M / Ali, Marwa / Abd Elsamee, Aya M / Sayed, Safa Matbouly / Sherif, Nadine / Sharaf, Howida M / Alhadidy, Mohamed A / Matboli, Marwa

    Cells

    2021  Volume 10, Issue 11

    Abstract: 1) Background: The coronavirus (COVID-19) pandemic is still a major global health problem, despite the development of several vaccines and diagnostic assays. Moreover, the broad symptoms, from none to severe pneumonia, and the various responses to ... ...

    Abstract (1) Background: The coronavirus (COVID-19) pandemic is still a major global health problem, despite the development of several vaccines and diagnostic assays. Moreover, the broad symptoms, from none to severe pneumonia, and the various responses to vaccines and the assays, make infection control challenging. Therefore, there is an urgent need to develop non-invasive biomarkers to quickly determine the infection severity. Circulating RNAs have been proven to be potential biomarkers for a variety of diseases, including infectious ones. This study aimed to develop a genetic network related to cytokines, with clinical validation for early infection severity prediction. (2) Methods: Extensive analyses of in silico data have established a novel IL11RA molecular network (IL11RNA mRNA, LncRNAs RP11-773H22.4 and hsa-miR-4257). We used different databases to confirm its validity. The differential expression within the retrieved network was clinically validated using quantitative RT-PCR, along with routine assessment diagnostic markers (CRP, LDH, D-dimmer, procalcitonin, Ferritin), in100 infected subjects (mild and severe cases) and 100 healthy volunteers. (3) Results: IL11RNA mRNA and LncRNA RP11-773H22.4, and the IL11RA protein, were significantly upregulated, and there was concomitant downregulation of hsa-miR-4257, in infected patients, compared to the healthy controls, in concordance with the infection severity. (4) Conclusion: The in-silico data and clinical validation led to the identification of a potential RNA/protein signature network for novel predictive biomarkers, which is in agreement with ferritin and procalcitonin for determination of COVID-19 severity.
    MeSH term(s) Adult ; Biomarkers/blood ; COVID-19/diagnosis ; COVID-19/genetics ; COVID-19/metabolism ; Computational Biology ; Female ; Gene Regulatory Networks ; Humans ; Interleukin-11 Receptor alpha Subunit/blood ; Interleukin-11 Receptor alpha Subunit/genetics ; Male ; MicroRNAs/blood ; MicroRNAs/genetics ; RNA, Long Noncoding/blood ; RNA, Long Noncoding/genetics ; RNA, Messenger/blood ; RNA, Messenger/genetics ; ROC Curve ; SARS-CoV-2/isolation & purification ; Severity of Illness Index
    Chemical Substances Biomarkers ; IL11RA protein, human ; Interleukin-11 Receptor alpha Subunit ; MicroRNAs ; RNA, Long Noncoding ; RNA, Messenger
    Language English
    Publishing date 2021-11-09
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10113098
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: ABHD4-Regulating RNA Panel: Novel Biomarkers in Acute Coronary Syndrome Diagnosis.

    Agwa, Sara H A / Elzahwy, Sherif Samir / El Meteini, Mahmoud Shawky / Elghazaly, Hesham / Saad, Maha / Abd Elsamee, Aya M / Shamekh, Rania / Matboli, Marwa

    Cells

    2021  Volume 10, Issue 6

    Abstract: Background: Acute coronary syndrome (ACS) is a major cause of death all over the world. STEMI represents a type of myocardial infarction with acute ST elevation. We aimed to assess the predictive power of potential RNA panel expression in acute coronary ...

    Abstract Background: Acute coronary syndrome (ACS) is a major cause of death all over the world. STEMI represents a type of myocardial infarction with acute ST elevation. We aimed to assess the predictive power of potential RNA panel expression in acute coronary syndrome.
    Method: We used in silico data analysis to retrieve RNAs related to glycerophospholipid metabolism dysregulation and specific to ACS that results in the selection of Alpha/Beta hydrolase fold domain4 (
    Results: The study data showed significant down regulation in the expression of the serum levels of
    Conclusion: Collectively,
    MeSH term(s) Acute Coronary Syndrome/blood ; Acute Coronary Syndrome/diagnosis ; Biomarkers/blood ; Gene Expression Regulation ; Humans ; Lysophospholipase ; MicroRNAs/blood ; RNA, Long Noncoding/blood ; RNA, Messenger/blood
    Chemical Substances Biomarkers ; MicroRNAs ; RNA, Long Noncoding ; RNA, Messenger ; ABHD4 protein, human (EC 3.1.1.-) ; Lysophospholipase (EC 3.1.1.5)
    Language English
    Publishing date 2021-06-16
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2661518-6
    ISSN 2073-4409 ; 2073-4409
    ISSN (online) 2073-4409
    ISSN 2073-4409
    DOI 10.3390/cells10061512
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article: Association between Interferon-Lambda-3 rs12979860, TLL1 rs17047200 and DDR1 rs4618569 Variant Polymorphisms with the Course and Outcome of SARS-CoV-2 Patients

    Agwa, Sara H. A. / Kamel, Marwa Mostafa / Elghazaly, Hesham / Abd Elsamee, Aya M. / Hafez, Hala / Girgis, Samia Abdo / Ezz Elarab, Hoda / Ebeid, Fatma S. E. / Sayed, Safa Matbouly / Sherif, Lina / Matboli, Marwa

    Genes. 2021 May 28, v. 12, no. 6

    2021  

    Abstract: Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection provides a critical host-immunological challenge. Aim: We explore the effect of host-genetic variation in interferon-lambda-3 rs12979860, Tolloid Like–1 (TLL1) rs17047200 ... ...

    Abstract Background: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection provides a critical host-immunological challenge. Aim: We explore the effect of host-genetic variation in interferon-lambda-3 rs12979860, Tolloid Like–1 (TLL1) rs17047200 and Discoidin domain receptor 1(DDR1) rs4618569 on host response to respiratory viral infections and disease severity that may probe the mechanistic approach of allelic variation in virus-induced inflammatory responses. Methods: 141 COVID-19 positive patients and 100 healthy controls were tested for interferon-lambda-3 rs12979860, TLL1 rs17047200 and DDR1 rs4618569 polymorphism by TaqMan probe-based genotyping. Different genotypes were assessed regarding the COVID-19 severity and prognosis. Results: There were statistically significant differences between the studied cases and control group with regard to the presence of comorbidities, total leucocytic count, lymphocytic count, CRP, serum LDH, ferritin and D-dimer (p < 0.01). The CC genotype of rs12979860 cytokine, the AA genotype of TLL1 rs17047200 and the AA genotype of the rs4618569 variant of DDR1 showed a higher incidence of COVID-19 compared to the others. There were significant differences between the rs4618569 variant of DDR and the outcome of the disease, with the highest mortality in AG genotype 29 (60.4%) in comparison to 16 (33.3%) and 3 (6.2%) in the AA and GG genotypes, respectively (p = 0.007*), suggesting that the A allele is associated with a poor outcome in the disease. Conclusion: Among people who carry C and A alleles of SNPs IFN-λ rs12979860 and TLL1 rs17047200, respectively, the AG genotype of the DDR1 rs4618569 variant is correlated with a COVID-19 poor outcome. In those patients, the use of anti-IFN-λ 3, TLL1 and DDR1 therapy may be promising for personalized translational clinical practice.
    Keywords COVID-19 infection ; Severe acute respiratory syndrome coronavirus 2 ; alleles ; allelic variation ; blood serum ; cytokines ; disease severity ; ferritin ; genotype ; genotyping ; mortality ; people ; prognosis ; therapeutics
    Language English
    Dates of publication 2021-0528
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    ZDB-ID 2527218-4
    ISSN 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes12060830
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top