Article ; Online: First insight into the genetic population structure of Mycobacterium tuberculosis isolated from pulmonary tuberculosis patients in Egypt.
Tuberculosis (Edinburgh, Scotland)
2016 Volume 96, Page(s) 13–20
Abstract: The present study aimed to assess the population structure of Mycobacterium tuberculosis (MTB) isolates from Egypt. A total of 230 MTB isolates were analysed using spoligotyping, large sequence polymorphism (LSPs), mycobacterial interspersed repetitive ... ...
Abstract | The present study aimed to assess the population structure of Mycobacterium tuberculosis (MTB) isolates from Egypt. A total of 230 MTB isolates were analysed using spoligotyping, large sequence polymorphism (LSPs), mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) typing and multi-locus sequence typing (MLST). The majority of isolates (93.0%) belonged to lineage 4, including 44.3, 13.4 and 10.8% of the ill-defined T clade, LAM and Haarlem families, respectively, and lineage 3 was identified in 7.0% of the isolates. MIRU-VNTRs typing allowed efficient discrimination of the spoligotype-defined clusters, including spoligo-international types (SIT) 53, 34, and 4, into 56 patterns, including 13 clusters and 43 unique patterns. A new SNP at position 311614 was identified in all six isolates to form the biggest MIRU-VNTR cluster, which suggested a recent clonal expansion. This SNP could possibly be used as a genetic marker for robust discriminations of Egyptian MTB isolates belonging to SIT53. The combination of spoligotyping, 12 MIRU-VNTRs loci and MLST provided insight into the genetic diversity and transmission dynamics of the Egyptian MTB genotypes and could be a key to implementation of effective control measures by public health authorities. |
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MeSH term(s) | Bacteriological Techniques ; DNA, Bacterial/genetics ; Egypt/epidemiology ; Evolution, Molecular ; Genotype ; Humans ; Interspersed Repetitive Sequences ; Minisatellite Repeats ; Molecular Epidemiology ; Mycobacterium tuberculosis/genetics ; Mycobacterium tuberculosis/isolation & purification ; Mycobacterium tuberculosis/pathogenicity ; Phenotype ; Phylogeny ; Polymorphism, Single Nucleotide ; Sputum/microbiology ; Tuberculosis, Pulmonary/epidemiology ; Tuberculosis, Pulmonary/microbiology |
Chemical Substances | DNA, Bacterial |
Language | English |
Publishing date | 2016-01 |
Publishing country | Scotland |
Document type | Journal Article |
ZDB-ID | 2046804-0 |
ISSN | 1873-281X ; 1472-9792 |
ISSN (online) | 1873-281X |
ISSN | 1472-9792 |
DOI | 10.1016/j.tube.2015.11.002 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
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