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  1. Article: The Influence of Different Culture Media on the Growth and Recombinant Protein Production of Iranian Lizard Leishmania Promastigote.

    Abdi Ghavidel, Afshin / Aghamiri, Shahin / Jajarmi, Vahid / Bandehpour, Mojgan / Kazemi, Bahram

    Iranian journal of parasitology

    2023  Volume 17, Issue 4, Page(s) 543–553

    Abstract: Background: Leishmania: Methods: We investigated the growth rate of the wild and recombinant I.L.L. in BHI, RPMI: Results: The growth rate of I.L.L. in RPMI1640 medium and LB broth was similar and supplementation with 10% FBS did not affect the ... ...

    Abstract Background: Leishmania
    Methods: We investigated the growth rate of the wild and recombinant I.L.L. in BHI, RPMI
    Results: The growth rate of I.L.L. in RPMI1640 medium and LB broth was similar and supplementation with 10% FBS did not affect the growth rate. The amount of protein expression in the LB medium was higher than in the other three media.
    Conclusion: The LB broth is an appropriate medium for I.L.L. culture and recombinant protein production.
    Language English
    Publishing date 2023-01-20
    Publishing country Iran
    Document type Journal Article
    ZDB-ID 2388453-8
    ISSN 2008-238X ; 1735-7020
    ISSN (online) 2008-238X
    ISSN 1735-7020
    DOI 10.18502/ijpa.v17i4.11282
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review.

    Abdi Ghavidel, Afshin / Jajarmi, Vahid / Bandehpour, Mojgan / Kazemi, Bahram

    Iranian journal of parasitology

    2022  Volume 17, Issue 3, Page(s) 286–295

    Abstract: Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A ... ...

    Abstract Protein complexes are involved in many vital biological processes. Therefore, researchers need these protein complexes for biochemical and biophysical studies. Several methods exist for expressing multi-subunit proteins in eukaryotic cells, such as 2A sequences, IRES, or intein. Nevertheless, each of these elements has several disadvantages that limit their usage. In this article, we suggest a new system for expressing multi-subunit proteins, which have several advantages over existing methods meanwhile it, lacks most of their disadvantages.
    Language English
    Publishing date 2022-11-18
    Publishing country Iran
    Document type Journal Article ; Review
    ZDB-ID 2388453-8
    ISSN 2008-238X ; 1735-7020
    ISSN (online) 2008-238X
    ISSN 1735-7020
    DOI 10.18502/ijpa.v17i3.10618
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Effectiveness of Coronavirus Vaccines against Syndrome Coronavirus 2 (SARS-CoV-2) and Its New Variants.

    Abdi Ghavidel, Afshin / Rojhannezhad, Mahbubeh / Kazemi, Bahram / Bandehpour, Mojgan

    Iranian journal of allergy, asthma, and immunology

    2021  Volume 20, Issue 6, Page(s) 647–671

    Abstract: The widespread outbreak of coronavirus disease 2019 in late 2019 caused many people worldwide to die or suffer from certain clinical complications even after the recovery. The virus has many social and economic adverse effects. Studies on severe acute ... ...

    Abstract The widespread outbreak of coronavirus disease 2019 in late 2019 caused many people worldwide to die or suffer from certain clinical complications even after the recovery. The virus has many social and economic adverse effects. Studies on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) have specified that spike, surface glycoprotein antigen, is considered as a major target to stimulate the immune system. This glycoprotein binds to the angiotensin-converting enzyme 2 on the surface of human cells especially lung epithelial cells and facilitates the virus entry. Therefore, the immune response stimulated by vaccination targeting this antigen may cause immunity against the whole virus. Currently, many companies are working on SARS-CoV-2 vaccines. They include 'traditional' vaccines like attenuated or inactivated virus platforms as well as the brand-new generations of vaccines such as viral vector-based, subunit, nucleic acid-based, and virus-like particle vaccines. Certainly, each vaccine platform presents several advantages and disadvantages affecting its efficacy and safety which is the main topic of this paper.
    MeSH term(s) Animals ; COVID-19 Vaccines/immunology ; Humans ; Mass Vaccination ; SARS-CoV-2/physiology ; Spike Glycoprotein, Coronavirus/immunology ; Vaccine Development ; Vaccine Efficacy ; Virus Internalization
    Chemical Substances COVID-19 Vaccines ; Spike Glycoprotein, Coronavirus ; spike glycoprotein, SARS-CoV
    Language English
    Publishing date 2021-12-08
    Publishing country Iran
    Document type Journal Article ; Review
    ZDB-ID 2488724-9
    ISSN 1735-5249 ; 1735-1502
    ISSN (online) 1735-5249
    ISSN 1735-1502
    DOI 10.18502/ijaai.v20i6.8016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 6806: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Leishmania Strains.

    Abdi Ghavidel, Afshin / Aghamiri, Shahin / Raee, Pourya / Mohammadi-Yeganeh, Samira / Noori, Effat / Bandehpour, Mojgan / Kazemi, Bahram / Jajarmi, Vahid

    Acta parasitologica

    2023  Volume 69, Issue 1, Page(s) 121–134

    Abstract: Background: Genome manipulation of Leishmania species and the creation of modified strains are widely employed strategies for various purposes, including gene function studies, the development of live attenuated vaccines, and the engineering of host ... ...

    Abstract Background: Genome manipulation of Leishmania species and the creation of modified strains are widely employed strategies for various purposes, including gene function studies, the development of live attenuated vaccines, and the engineering of host cells for protein production.
    Objective: Despite the introduction of novel manipulation approaches like CRISPR/Cas9 technology with significant advancements in recent years, the development of a reliable protocol for efficiently and precisely altering the genes of Leishmania strains remains a challenging endeavor. Following the successful adaptation of the CRISPR/Cas9 system for higher eukaryotic cells, several research groups have endeavored to apply this system to manipulate the genome of Leishmania.
    Results: Despite the substantial differences between Leishmania and higher eukaryotes, the CRISPR/Cas9 system has been effectively tested and applied in Leishmania.  CONCLUSION: This comprehensive review summarizes all the CRISPR/Cas9 systems that have been employed in Leishmania, providing details on their methods and the expression systems for Cas9 and gRNA. The review also explores the various applications of the CRISPR system in Leishmania, including the deletion of multicopy gene families, the development of the Leishmania vaccine, complete gene deletions, investigations into chromosomal translocations, protein tagging, gene replacement, large-scale gene knockout, genome editing through cytosine base replacement, and its innovative use in the detection of Leishmania. In addition, the review offers an up-to-date overview of all double-strand break repair mechanisms in Leishmania.
    MeSH term(s) Leishmania/genetics ; Gene Editing/methods ; CRISPR-Cas Systems ; Genome, Protozoan ; Leishmaniasis/parasitology ; Animals
    Language English
    Publishing date 2023-12-21
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 1132735-2
    ISSN 1896-1851 ; 0065-1478 ; 1230-2821
    ISSN (online) 1896-1851
    ISSN 0065-1478 ; 1230-2821
    DOI 10.1007/s11686-023-00756-0
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uh II Zs.224: Show issues Location:
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    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: The engineered probiotics for the treatment of chronic diseases: A systematic review

    Barati, Meisam / Jabbari, Masoumeh / Abdi Ghavidel, Afshin / Nikmehr, Payman / Arzhang, Pishva / Aynehchi, Aydin / Babashahi, Mina / Mosharkesh, Erfan / Roshanravan, Neda / Shabani, Mahdi / Davoodi, Sayed Hossein

    Journal of Food Biochemistry. 2022 Oct., v. 46, no. 10 p.e14343-

    2022  

    Abstract: Engineered probiotics (EPs) are a group of probiotics whose proteome is manipulated by biotechnological techniques. EPs have attracted a lot of attention in recent researches for preventing and treating chronic diseases. The current study has been ... ...

    Abstract Engineered probiotics (EPs) are a group of probiotics whose proteome is manipulated by biotechnological techniques. EPs have attracted a lot of attention in recent researches for preventing and treating chronic diseases. The current study has been conducted to provide an overview regarding the EPs application in the treatment of chronic disease by a comprehensive systematic review of the published articles up to January 2022. To retrieve the related publications, three databases (PubMed/MEDLINE, Web of Sciences, and Scopus) were searched systematically. Finally, all human (n = 2) and animal (n = 37) studies were included. The included articles evaluated the effects of EPs on treatment of arthritis (n = 3), cancer (n = 2), autoimmune encephalomyelitis (EAE; n = 6), Parkinson disease (PD; n = 1), Alzheimer diseases (AD; n = 1), colitis (n = 11), celiac disease (n = 1), diabetes (n = 8) and cardiovascular disease (CVD; n = 6). Induction of oral tolerance (OT) is the most important mechanism of EPs action in the treatment of chronic disease. Providing oral vaccine and bioactive compounds are the other mechanisms of EPs action. PRACTICAL APPLICATIONS: The current systematic review gathered evidence about the application of EPs in the treatment of chronic diseases. Evidence suggests that EPs have very broad and potent effects in the treatment of chronic and even genetic diseases.
    Keywords Parkinson disease ; arthritis ; biochemistry ; cardiovascular diseases ; celiac disease ; chronic diseases ; colitis ; diabetes ; edible vaccines ; encephalitis ; humans ; probiotics ; proteome ; systematic review
    Language English
    Dates of publication 2022-10
    Publishing place John Wiley & Sons, Ltd
    Document type Article ; Online
    Note REVIEW
    ZDB-ID 433846-7
    ISSN 1745-4514 ; 0145-8884
    ISSN (online) 1745-4514
    ISSN 0145-8884
    DOI 10.1111/jfbc.14343
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

    Z 3310: Show issues

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  6. Article ; Online: The engineered probiotics for the treatment of chronic diseases: A systematic review.

    Barati, Meisam / Jabbari, Masoumeh / Abdi Ghavidel, Afshin / Nikmehr, Payman / Arzhang, Pishva / Aynehchi, Aydin / Babashahi, Mina / Mosharkesh, Erfan / Roshanravan, Neda / Shabani, Mahdi / Davoodi, Sayed Hossein

    Journal of food biochemistry

    2022  Volume 46, Issue 10, Page(s) e14343

    Abstract: Engineered probiotics (EPs) are a group of probiotics whose proteome is manipulated by biotechnological techniques. EPs have attracted a lot of attention in recent researches for preventing and treating chronic diseases. The current study has been ... ...

    Abstract Engineered probiotics (EPs) are a group of probiotics whose proteome is manipulated by biotechnological techniques. EPs have attracted a lot of attention in recent researches for preventing and treating chronic diseases. The current study has been conducted to provide an overview regarding the EPs application in the treatment of chronic disease by a comprehensive systematic review of the published articles up to January 2022. To retrieve the related publications, three databases (PubMed/MEDLINE, Web of Sciences, and Scopus) were searched systematically. Finally, all human (n = 2) and animal (n = 37) studies were included. The included articles evaluated the effects of EPs on treatment of arthritis (n = 3), cancer (n = 2), autoimmune encephalomyelitis (EAE; n = 6), Parkinson disease (PD; n = 1), Alzheimer diseases (AD; n = 1), colitis (n = 11), celiac disease (n = 1), diabetes (n = 8) and cardiovascular disease (CVD; n = 6). Induction of oral tolerance (OT) is the most important mechanism of EPs action in the treatment of chronic disease. Providing oral vaccine and bioactive compounds are the other mechanisms of EPs action. PRACTICAL APPLICATIONS: The current systematic review gathered evidence about the application of EPs in the treatment of chronic diseases. Evidence suggests that EPs have very broad and potent effects in the treatment of chronic and even genetic diseases.
    MeSH term(s) Animals ; Chronic Disease ; Humans ; Probiotics ; Proteome
    Chemical Substances Proteome
    Language English
    Publishing date 2022-07-26
    Publishing country United States
    Document type Journal Article ; Review ; Systematic Review ; Research Support, Non-U.S. Gov't
    ZDB-ID 433846-7
    ISSN 1745-4514 ; 0145-8884
    ISSN (online) 1745-4514
    ISSN 0145-8884
    DOI 10.1111/jfbc.14343
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Bonn / Germany

    Z 3310: Show issues

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

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