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Article ; Online: Fabrication of mesoporous silica nanoparticles for targeted delivery of sunitinib to ovarian cancer cells

Mitra Torabi / Ayuob Aghanejad / Pouria Savadi / Abolfazl Barzegari / Yadollah Omidi / Jaleh Barar

BioImpacts, Vol 13, Iss 3, Pp 255-

2023 Volume 267

Abstract: Introduction: Mesoporous silica nanoparticles (MSNPs) are considered innovative multifunctional structures for targeted drug delivery owing to their outstanding physicochemical characteristics. Methods: MSNPs were fabricated using the sol-gel method, and ...

Abstract	Introduction: Mesoporous silica nanoparticles (MSNPs) are considered innovative multifunctional structures for targeted drug delivery owing to their outstanding physicochemical characteristics. Methods: MSNPs were fabricated using the sol-gel method, and polyethylene glycol-600 (PEG600) was used for MSNPs modification. Subsequently, sunitinib (SUN) was loaded into the MSNPs, MSNP-PEG and MSNP-PEG/SUN were grafted with mucin 16 (MUC16) aptamers. The nanosystems (NSs) were characterized using FT-IR, TEM, SEM, DLS, XRD, BJH, and BET. Furthermore, the biological impacts of MSNPs were evaluated on the ovarian cancer cells by MTT assay and flow cytometry analysis. Results: The results revealed that the MSNPs have a spherical shape with an average dimension, pore size, and surface area of 56.10 nm, 2.488 nm, and 148.08 m2g-1, respectively. The cell viability results showed higher toxicity of targeted MSNPs in MUC16 overexpressing OVCAR-3 cells as compared to the SK-OV-3 cells; that was further confirmed by the cellular uptake results. The cell cycle analysis exhibited that the induction of sub-G1 phase arrest mostly occurred in MSNP-PEG/SUN-MUC16 treated OVCAR-3 cells and MSNP-PEG/SUN treated SK-OV-3 cells. DAPI staining showed apoptosis induction upon exposure to targeted MSNP in MUC16 positive OVCAR-3 cells. Conclusion: According to our results, the engineered NSs could be considered an effective multifunctional targeted drug delivery platform for the mucin 16 overexpressing cells.
Keywords	ovarian cancer ; mucin 16 aptamer ; mesoporous silica nanoparticles ; targeted drug delivery ; sunitinib ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Designing a light-activated recombinant alpha hemolysin for colorectal cancer targeting

Siamak Alizadeh / Abolfazl Barzegari / Abolghasem Esmaeili / Yadollah Omidi

BioImpacts, Vol 10, Iss 3, Pp 187-

2020 Volume 193

Abstract: Introduction: Colorectal cancer (CRC) is one of the main health burden worldwide, which can cause major economic and physiological problems along with relatively high rate of mortality. It is important to develop new methods for the localized delivery of ...

Abstract	Introduction: Colorectal cancer (CRC) is one of the main health burden worldwide, which can cause major economic and physiological problems along with relatively high rate of mortality. It is important to develop new methods for the localized delivery of recombinant protein therapeutics, in large part due to the failure of conventional therapies in most cases. Since E. coli Nissle 1917 (EcN) does not produce any virulence factors, here we used these bacteria with the light-activated promoter system to deliver therapeutic agents in the desired location and time. Methods: In this study, Staphylococcus aureus alpha hemolysin (SAH), after codon usage optimization, was cloned into blue light activating vector (pDawn) and transferred to EcN strain. Then, the functionality and cytotoxicity of secreted alpha hemolysin was evaluated in the SW480 colon cancer cell line by using different experiments, including blood agar test, flow cytometry analysis, and DAPI staining. Results: Our findings revealed that EcN can produce functional SAH under the blue light irradiation against SW480 cancer cells. Moreover, cytotoxicity assays confirmed the dose- and time-dependent toxicity of this payload (SAH) against SW480 cancer cells. Conclusion: Based on our results, EcN is proposed as an appropriate light-activated vehicle for delivery of anticancer agents to the target cancer cells/tissues.
Keywords	alpha hemolysin ; colon cancer ; e. coli nissle 1917 ; light-activated vector ; staphylococcus aureus ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Hollow pollen grains as scaffolding building blocks in bone tissue engineering

Solmaz Zakhireh / Jaleh Barar / Younes Beygi-Khosrowshahi / Abolfazl Barzegari / Yadollah Omidi / Khosro Adibkia

BioImpacts, Vol 12, Iss 3, Pp 183-

2022 Volume 193

Abstract: Introduction: The current study, for the first time, suggests nature-made pollen grains (PGs) of Pistacia vera L. as a potential candidate for using as scaffolding building blocks with encapsulation capability of bioactive compounds, such as bone ... ...

Abstract	Introduction: The current study, for the first time, suggests nature-made pollen grains (PGs) of Pistacia vera L. as a potential candidate for using as scaffolding building blocks with encapsulation capability of bioactive compounds, such as bone morphogenetic protein 4 (BMP4). Methods: A modified method using KOH (5%, 25ºC) was developed to produce nonallergic hollow pollen grains (HPGs), confirmed by energy dispersive X-ray (EDX) analysis, field emission scanning electron microscopy (FESEM), and DNA and protein staining techniques. The in-vitro study was conducted on human adipose-derived mesenchymal stem cells (hAD-MSCs) to investigate the applicability of HPGs as bone scaffolding building blocks. Cytocompability was evaluated by FESEM, MTT assay, and gene expression analysis of apoptotic markers (BAX and BCL2). The osteoconductive potential of HPGs was assessed by alkaline phosphatase (ALP) activity measurement and gene expression analysis of osteogenic markers (RUNX2 and osteocalcin). Results: Findings demonstrated that HPGs can be considered as biocompatible compounds increasing the metabolic activities of the cells. Further, the bioactive nature of HPGs resulted in suitable cellular adhesion properties, required for a potent scaffold. The investigation of apoptotic gene expression indicated a reduced BAX/BCL2 ratio reflecting the protective effect of HPGs on hAD-MSCs. The increased ALP activity and expression of osteogenic genes displayed the osteoconductive property of HPGs. Moreover, the incorporation of BMP4 in HPGs initiated a synergistic effect on osteoblast maturation. Conclusion: Owing to the unique compositional and surface nanotopographical features of the Pistacia vera L. HPG, this microscale architecture provides a favorable microenvironment for the bottom-up remodeling of bone.
Keywords	pollen grain ; pistacia vera l ; bottom-up tissue engineering ; building block ; bone tissue ; human adipose-derived mesenchymal stem cells ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	616
Language	English
Publishing date	2022-05-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Carotenoids as signaling molecules in cardiovascular biology

Abolfazl Barzegari / Graciela Pavon-Djavid

BioImpacts, Vol 4, Iss 3, Pp 111-

2014 Volume 112

Abstract: Oxidative stress and inflammation play important roles in the etiology of cardiovascular disease (CVD). Thus, natural antioxidant carotenoids existing in fruits and vegetables could have a significant role in the prevention of CVD. Nevertheless,clinical ... ...

Abstract	Oxidative stress and inflammation play important roles in the etiology of cardiovascular disease (CVD). Thus, natural antioxidant carotenoids existing in fruits and vegetables could have a significant role in the prevention of CVD. Nevertheless,clinical data are conflicting about the positive effect of some antioxidant carotenoids in reducing cardiovascular morbidity and mortality. Many biological actions of carotenoids have been attributed to their antioxidant effect; however, the precise mechanism by which carotenoids produce their beneficial effects is still under discussion. They might modulate molecular pathways involved in cell proliferation, acting at Akt, tyrosine kinases, mitogen activated protein kinase (MAP kinase) and growth factor signaling cascades. Screening for a promising cardiovascular protective carotenoids therefore might be performed in vitro and in vivo with caution in cross-interaction with other molecules involved in signaling pathways especially those affecting microRNAs, performing a role in molecular modulation of cardiovascular cells.
Keywords	Carotenoids ; Cardiovascular disease ; MicroRNA ; Reactive oxygen species (ROS) ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	500
Language	English
Publishing date	2014-09-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Efficient and stable transformation of Dunaliella pseudosalina by 3 strains of Agrobacterium tumefaciens

Jaber Dehghani / Ali Movafeghi / Abolfazl Barzegari / Jaleh Barar

BioImpacts, Vol 7, Iss 4, Pp 247-

2017 Volume 254

Abstract: Introduction: Several platforms including mammalian, plant and insect cells as well as bacteria, yeasts, and microalgae are available for the production of recombinant proteins. Low efficiency of delivery systems, extracellular and intracellular ... ...

Abstract	Introduction: Several platforms including mammalian, plant and insect cells as well as bacteria, yeasts, and microalgae are available for the production of recombinant proteins. Low efficiency of delivery systems, extracellular and intracellular degradation of foreign genes during transformation, difficulties in targeting and importing into the nucleus, and finally problems in integration into nuclear genome are the most bottlenecks of classical plasmids for producing recombinant proteins. Owing to high growth rate, no common pathogen with humans, being utilized as humans’ food, and capability to perform N-glycosylation, microalgae are proposed as an ideal system for such biotechnological approaches. Here, Agrobacterium tumefaciens is introduced as an alternative tool for transformation of the microalga Dunaliella pseudosalina. Methods: The transformation of gfp gene into the D. pseudosalina was evaluated by three strains including EHA101, GV3301 and GV3850 of A. tumefaciens. The integrating and expression of gfp gene were determined by PCR, RT-PCR, Q-PCR and SDS-PAGE analyses. Results: The T-DNA of pCAMBIA1304 plasmid was successfully integrated into the genome of the microalgal cells. Although all of the strains were able to transform the algal cells, GV3301 possessed higher potential to transform the microalgal cells in comparison to EHA101 and GV3850 strains. Moreover, the stability of gfp gene was successfully established during a course of two months period in the microalgal genome. Conclusion: Agrobacterium is introduced as a competent system for stable transformation of Dunaliella strains in order to produce eukaryotic recombinant proteins.
Keywords	agrobacterium tumefaciens ; dunaliella pseudosalina ; gene expression ; microalgal transformation ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	570
Language	English
Publishing date	2017-09-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Effect of Selenium and Electrical Stimulation on the Expression of Cation Channel Spermia 1 and 2 Genes in Spinal Cord Injury Rat Model

Soheila Bani / Iraj Lotfinia / Parviz Shahabi / Abolfazl Barzegari / Jalal Abdolalizadeh / Amir Vahedi / Meysam Ghorbani

Crescent Journal of Medical and Biological Sciences, Vol 7, Iss 3, Pp 382-

2020 Volume 388

Abstract: Objectives: Cation Channel Spermia (CatSper) 1-4 are considered as a unique family of "sperm cation channels" and play an important role in the motility of the sperm and male fertility. The aim of this study was to compare and evaluate the effects of ... ...

Abstract	Objectives: Cation Channel Spermia (CatSper) 1-4 are considered as a unique family of "sperm cation channels" and play an important role in the motility of the sperm and male fertility. The aim of this study was to compare and evaluate the effects of selenium (Se) and electrical stimulation (Es) on the expression of CatSper 1 and 2 genes in the spinal cord injury (SCI) rat model. Materials and Methods: The present experimental study was performed on 50 male Wistar rats. The animals were randomly divided into 5 groups of 10 which experienced SCI using an SCI impactor device. Forty-eight hours after injury, the Se group received Se for 6 consecutive days for 6 weeks, and Es was performed for 1 hour per day for 6 consecutive days during 6 weeks in the Es group. Finally, the real-time polymerase chain reaction (PCR) was performed for the expression of CatSper 1 and 2 genes, followed by using one-way ANOVA for the analysis of gene expression data. The results were considered statistically significant when P < 0.05. Results: Se treatment showed an increase in the relative expression of CatSper 1 and 2 genes compared to the control group, but this increase was higher for CatSper 2. Eventually, Es did not affect the relative intensity of CatSper 1 and 2 gene expression after SCI. Conclusions: Se, more than Es, could increase the relative intensity of CatSper1 and 2 expressions, and this increase was higher in CatSper2.
Keywords	selenium ; electrical stimulation ; catsper 1 and 2 genes ; Medicine ; R
Subject code	572
Language	English
Publishing date	2020-07-01T00:00:00Z
Publisher	Aras Part Medical International Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody from a Phage Display Library

Shirafkan Kordi / Mohammad Rahmati-Yamchi / Mehdi Asghari Vostakolaei / Ali Etemadie / Abolfazl Barzegari / Jalal Abdolalizadeh

Iranian Journal of Allergy, Asthma and Immunology, Vol 18, Iss

2019 Volume 3

Abstract: Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody ... ...

Abstract	Vascular endothelial growth factor receptor 2 (VEGFR-2) is known as one of the important antigens playing a vital role in angiogenesis. In this study, phage display technology (PDT) was used to produce a single-chain variable fragment (scFv) antibody against a region of the domain 3 in VEGFR-2 called kinase insert domain receptor 3 (KDR3). After designing the KDR3 peptide and biopanning, a colony was chosen for scFv antibody expression. Following expression and purification; western blotting, dot blotting and immunofluorescence (IF) were used to evaluate the antibody function. Surface plasmon resonance (SPR) was also employed to measure affinity of produced antibody. Once a colony was selected and transferred to the expression host, the scFv antibody was expressed in the expected range of 28 kDa. Using a designed chromatography column, antibody purification was found to be about 95%. In this study, a novel scFv with the capability of binding to KDR3 was isolated and purified and its intracellular function was investigated and verified.
Keywords	Monoclonal antibody ; Kinase insert domain receptor 3 (KDR3) ; Phage display ; Single-chain variable fragment (SCFV) ; Vascular endothelial growth factor receptor-2 (VEGFR2) ; Medicine ; R
Subject code	572
Language	English
Publishing date	2019-06-01T00:00:00Z
Publisher	Tehran University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: An Update to Space Biomedical Research

Abolfazl Barzegari / Amir Ata Saei

BioImpacts, Vol 2, Iss 1, Pp 23-

Tissue Engineering in Microgravity Bioreactors

2012 Volume 32

Abstract: Introduction: The severe need for constructing replacement tissues in organ transplantation has necessitated the development of tissue engineering approaches and bioreactors that can bring these approaches to reality. The inherent limitations of ... ...

Abstract	Introduction: The severe need for constructing replacement tissues in organ transplantation has necessitated the development of tissue engineering approaches and bioreactors that can bring these approaches to reality. The inherent limitations of conventional bioreactors in generating realistic tissue constructs led to the devise of the microgravity tissue engineering that uses Rotating Wall Vessel (RWV) bioreactors initially developed by NASA. Methods: In this review article, we intend to highlight some major advances and accomplishments in the rapidly-growing field of tissue engineering that could not be achieved without using microgravity. Results: Research is now focused on assembly of 3 dimensional (3D) tissue fragments from various cell types in human body such as chondrocytes, osteoblasts, embryonic and mesenchymal stem cells, hepatocytes and pancreas islet cells. Hepatocytes cultured under microgravity are now being used in extracorporeal bioartificial liver devices. Tissue constructs can be used not only in organ replacement therapy, but also in pharmaco-toxicology and food safety assessment. 3D models of various cancers may be used in studying cancer development and biology or in high-throughput screening of anticancer drug candidates. Finally, 3D heterogeneous assemblies from cancer/immune cells provide models for immunotherapy of cancer. Conclusion: Tissue engineering in (simulated) microgravity has been one of the stunning impacts of space research on biomedical sciences and their applications on earth.
Keywords	Tissue Engineering ; Aerospace Medicine ; Bioreactors ; Microgravity ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	621
Language	English
Publishing date	2012-03-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Lactobacillus plantarum induces apoptosis in gastric cancer cells via modulation of signaling pathways in Helicobacter pylori

Hadi Maleki-Kakelar / Jaber Dehghani / Abolfazl Barzegari / Jaleh Barar / Masoud Shirmohamadi / Javid Sadeghi / Yadollah Omidi

BioImpacts, Vol 10, Iss 2, Pp 65-

2020 Volume 72

Abstract: Introduction: Gastric cancer is considered the second prevalent cause of death around the world. This type of cancer is generally induced by Helicobacter pylori which could colonize within the gastric mucosa of the infected cases. To date, triple ... ...

Abstract	Introduction: Gastric cancer is considered the second prevalent cause of death around the world. This type of cancer is generally induced by Helicobacter pylori which could colonize within the gastric mucosa of the infected cases. To date, triple antibiotic therapy has routinely been utilized for controlling the H. pylori-induced infection. However, this strategy has been unsuccessful, in large part because of issues such as occurring point mutations in the H. pylori genome that can induce resistance to the antibiotics administered. Recently, it has been shown that different probiotics may have strong anti-cancer effects, in which they are capable of inhibiting H. pylori by both immunological and non-immunological mechanisms. Here, we aimed at finding possible anti-cancer impacts of the probiotic bacterium Lactobacillus plantarum on gastric cancer, AGS cells. Methods: The anti-cancer effects of the conditioned media of the locally isolated L. plantarum on the AGS cells were evaluated by different analyses such as flow cytometry, DNA ladder assay, DAPI staining, and RT-PCR. Results: Our findings showed that the conditioned media of L. plantarum can inhibit both H. pylori and AGS cells through up-/down-regulation of PTEN, Bax, TLR4, and AKT genes. The exudates of the probiotic L. plantarum bacteria can increase the expression of PTEN, Bax, and TLR4, and also decrease the expression of AKT gene. Conclusion: In agreement with different reports, our results proved the anti-cancer effects of the locally isolated L. plantarum through some immunological cell signaling pathways. Accordingly, it seems the probiotics could be considered as at least a complementary treatment for different types of malignancies.
Keywords	gastric cancer ; helicobacter pylori ; lactobacillus plantarum ; apoptosis ; pten ; akt ; Medicine (General) ; R5-920 ; Biology (General) ; QH301-705.5
Subject code	610
Language	English
Publishing date	2020-03-01T00:00:00Z
Publisher	Tabriz University of Medical Sciences
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article ; Online: Correction

Leila Javadi / Mostafa Ghavami / Manouchehr Khoshbaten / Abdolrasoul Safaiyan / Abolfazl Barzegari / Bahram Pourghassem Gargari1

Crescent Journal of Medical and Biological Sciences, Vol 5, Iss 1, Pp 69-

The Potential Role of Probiotics or/and Prebiotic on Serum Lipid Profle and Insulin Resistance in Non-Alcoholic Fatty Liver Disease: A Double Blind Randomized Clinical Trial

2018 Volume 69

Keywords	Medicine ; R
Language	English
Publishing date	2018-01-01T00:00:00Z
Publisher	Aras Part Medical International Press
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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