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  1. Article ; Online: Combined effects of naringin and doxorubicin on the JAK/STAT signaling pathway reduce the development and spread of breast cancer cells.

    Effat, Heba / Abosharaf, Hamed A / Radwan, Aliaa M

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 2824

    Abstract: Breast cancer therapy options are limited due to its late diagnosis and poor prognosis. Doxorubicin is the fundamental therapy approach for this disease. Because chemotherapy has numerous adverse effects, the scope of the existing research was to ... ...

    Abstract Breast cancer therapy options are limited due to its late diagnosis and poor prognosis. Doxorubicin is the fundamental therapy approach for this disease. Because chemotherapy has numerous adverse effects, the scope of the existing research was to appraise the synergetic effect of doxorubicin and naringin and explore the underlying mechanism. The cytotoxicity of doxorubicin and naringin on MCF-7 was monitored. Furthermore, the expression of STAT3 and JAK1 as well as the apoptotic and metastatic related genes (Bax, Bcl-2, Survivin, and VEGF) were conducted by immunoblotting assay and qRT-PCR. In addition, a wound healing test was utilized to appraise the migration and metastasis of MCF-7. Our results revealed that naringin and doxorubicin had a synergetic inhibitory influence on MCF-7 cells growth and migration. The synergetic action of doxorubicin and naringin effectively hindered the expression of STAT3, JAK1, Bcl-2, Survivin, and VEGF, with a boost in the level of Bax compared to cells treated with either doxorubicin or naringin. In conclusion, our findings imply that combining doxorubicin with naringin may be a favorable strategy for inhibiting the growth of breast cancer.
    MeSH term(s) Humans ; Female ; Breast Neoplasms/pathology ; Survivin/metabolism ; bcl-2-Associated X Protein ; Vascular Endothelial Growth Factor A/pharmacology ; Apoptosis ; Doxorubicin/pharmacology ; Doxorubicin/therapeutic use ; Proto-Oncogene Proteins c-bcl-2/metabolism ; Signal Transduction ; Cell Line, Tumor ; Flavanones
    Chemical Substances Survivin ; naringin (N7TD9J649B) ; bcl-2-Associated X Protein ; Vascular Endothelial Growth Factor A ; Doxorubicin (80168379AG) ; Proto-Oncogene Proteins c-bcl-2 ; Flavanones
    Language English
    Publishing date 2024-02-03
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-53320-9
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Ehrlich ascites carcinoma provokes renal toxicity and DNA injury in mice: Therapeutic impact of chitosan and maitake nanoparticles.

    Abosharaf, Hamed A / Gebreel, Doaa T / Allam, Sahar / El-Atrash, Afaf / Tousson, Ehab

    Basic & clinical pharmacology & toxicology

    2024  Volume 134, Issue 4, Page(s) 472–484

    Abstract: In this study, the impact of chitosan (CS) and maitake (GF) nanoparticles towards the renal toxicity induced by Ehrlich ascites carcinoma (EAC) in vivo model was conducted. Besides benchmark negative control group, EAC model was constructed by ... ...

    Abstract In this study, the impact of chitosan (CS) and maitake (GF) nanoparticles towards the renal toxicity induced by Ehrlich ascites carcinoma (EAC) in vivo model was conducted. Besides benchmark negative control group, EAC model was constructed by intraperitoneal injection (i.p.) of 2.5 × 10
    MeSH term(s) Animals ; Mice ; Ascites/metabolism ; Chitosan/therapeutic use ; Grifola ; Carcinoma, Ehrlich Tumor/drug therapy ; Carcinoma, Ehrlich Tumor/pathology ; Creatinine ; DNA Damage ; Urea ; Apoptosis
    Chemical Substances Chitosan (9012-76-4) ; Creatinine (AYI8EX34EU) ; Urea (8W8T17847W)
    Language English
    Publishing date 2024-02-18
    Publishing country England
    Document type Journal Article
    ZDB-ID 2134679-3
    ISSN 1742-7843 ; 1742-7835
    ISSN (online) 1742-7843
    ISSN 1742-7835
    DOI 10.1111/bcpt.13988
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Uf I Zs.144: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
    ab Jg. 2022: Lesesaal (EG)

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  3. Article ; Online: Alzheimer's disease-related brain insulin resistance and the prospective therapeutic impact of metformin.

    Abosharaf, Hamed A / Elsonbaty, Yasmin / Tousson, Ehab / M Mohamed, Tarek

    Journal of neuroendocrinology

    2023  Volume 36, Issue 1, Page(s) e13356

    Abstract: Besides COVID-19, two of the most critical outbreaks of our day are insulin resistance, type 2 diabetes mellitus (T2DM), and Alzheimer's disease (AD). Each disease's pathophysiology is well established. Furthermore, a substantial overlap between them has ...

    Abstract Besides COVID-19, two of the most critical outbreaks of our day are insulin resistance, type 2 diabetes mellitus (T2DM), and Alzheimer's disease (AD). Each disease's pathophysiology is well established. Furthermore, a substantial overlap between them has coexisted. Uncertainty remains on whether T2DM and AD are parallel illnesses with the same origin or separate illnesses linked through violent pathways. The current study was aimed at testing whether the insulin resistance in the brain results in AD symptoms or not. Insulin resistance was induced in the brains of rats using a single intracerebroventricular streptozotocin (STZ) dose. We then measured glucose, insulin receptor substrate 2 (IRS-2), amyloid β (Aβ) deposition, and tau phosphorylation in the brain to look for signs of insulin resistance and AD. The results of this study indicated that a single dose of STZ was able to induce insulin resistance in the brain and significantly decline IRS-2. This resistance was accompanied by obvious memory loss, Aβ deposition, and tau phosphorylation, further visible diminishing in neurotransmitters such as dopamine and acetylcholine. Furthermore, oxidative stress was increased due to the antioxidant system being compromised. Interestingly, the pancreas injury and peripheral insulin resistance coexisted with brain insulin resistance. Indeed, the antidiabetic metformin was able to enhance all these drastic effects. In conclusion, brain insulin resistance could lead to AD and vice versa. These are highly linked syndromes that could influence peripheral organs. Further studies are required to stabilize this putative pathobiology relationship between them.
    MeSH term(s) Rats ; Animals ; Alzheimer Disease/metabolism ; Insulin Resistance/physiology ; Amyloid beta-Peptides/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Metformin/pharmacology ; Metformin/metabolism ; Insulin/metabolism ; Brain/metabolism ; Disease Models, Animal
    Chemical Substances Amyloid beta-Peptides ; Metformin (9100L32L2N) ; Insulin
    Language English
    Publishing date 2023-11-20
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1007517-3
    ISSN 1365-2826 ; 0953-8194
    ISSN (online) 1365-2826
    ISSN 0953-8194
    DOI 10.1111/jne.13356
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2634: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  4. Article ; Online: Synthesis, Characterization, and Anticancer Potency of Branched Poly (p-Hydroxy Styrene) Schiff-Bases.

    Kenawy, El-Refaie / El-Khalafy, Sahar H / Abosharaf, Hamed A / El-Nshar, Esraa M / Ghazy, Ahmed R / Azaam, Mohamed M

    Macromolecular bioscience

    2023  Volume 23, Issue 11, Page(s) e2300090

    Abstract: A significant issue in cancer biology is finding anticancer therapies that effectively kill cancer cells. Through the use of several aldehydes, Schiff bases based on branched poly (p-hydroxy styrene) are created. The branched polymer is first ... ...

    Abstract A significant issue in cancer biology is finding anticancer therapies that effectively kill cancer cells. Through the use of several aldehydes, Schiff bases based on branched poly (p-hydroxy styrene) are created. The branched polymer is first chloroacetylated, then aminated with 1,4-phenylenediamine, and finally, aldehydes are reacted with the aminated polymer to produce the Schiff base compounds. Through the utilization of FTIR, TGA, XRD, NMR, and elemental analysis, all synthesized Schiff-bases are identified and characterized. Further, the antineoplastic potential of all Schiff bases is evaluated against different cancer cell lines. The results gained from this study indicate that the Schiff base polymers have cytotoxic power against cancer cells depending on cancer cell type and this antiproliferation potency is dose-concentration dependent. Importantly, the prepared S1 Schiff-base polymer shows potent cytotoxicity and is able to trigger the apoptosis and reactive oxygen species (ROS) in MCF-7 cells. Further, it downregulates VEGFR protein expression. The Schiff base polymers would have extensive applications in the biological disciplines.
    MeSH term(s) Humans ; Schiff Bases/pharmacology ; Schiff Bases/chemistry ; MCF-7 Cells ; Antineoplastic Agents/pharmacology ; Antineoplastic Agents/chemistry ; Aldehydes ; Polymers ; Styrenes
    Chemical Substances Schiff Bases ; Antineoplastic Agents ; Aldehydes ; Polymers ; Styrenes
    Language English
    Publishing date 2023-07-10
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2039130-4
    ISSN 1616-5195 ; 1616-5187
    ISSN (online) 1616-5195
    ISSN 1616-5187
    DOI 10.1002/mabi.202300090
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Osthole extracted from a citrus fruit that affects apoptosis on A549 cell line by histone deacetylasese inhibition (HDACs).

    Abosharaf, Hamed A / Diab, Thoria / Atlam, Faten M / Mohamed, Tarek M

    Biotechnology reports (Amsterdam, Netherlands)

    2020  Volume 28, Page(s) e00531

    Abstract: This study aims to investigate the interactions between osthole extracted from Egyptian citrus fruits as HDACs inhibitor by theoretical study and practically. Besides, osthole was assed as anti-cancer activity. In this study, osthole was extracted from ... ...

    Abstract This study aims to investigate the interactions between osthole extracted from Egyptian citrus fruits as HDACs inhibitor by theoretical study and practically. Besides, osthole was assed as anti-cancer activity. In this study, osthole was extracted from the Egyptian citrus fruit and was characterized. The role of osthole as in vitro inhibitor of HDACs was estimated and evaluated the antitumor activity against human lung cancer cells (A549), Caspase-9 activity was detected. The results obtained from GC-MS indicate that the grapefruit showed the highest osthole concentration compared to the other citrus fruits. Moreover, the grapefruit osthole competitively inhibits HDACs. The inhibition constant value, (Ki=3.36 mM), indicates that osthole exerts an inhibitory effect upon HDACs activity. In vitro study of osthole could inhibit the growth of A549 cells that depend on time and concentration. It also induces apoptosis and causes an increase of caspase-9 by osthole. In conclusion, grapefruit osthole could induce the apoptosis in A549 lung cancer cells by inhibiting the histone deacetylase.
    Language English
    Publishing date 2020-09-22
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2020.e00531
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article: Osthole extracted from a citrus fruit that affects apoptosis on A549 cell line by histone deacetylasese inhibition (HDACs)

    Abosharaf, Hamed A / Diab, Thoria / Atlam, Faten M / Mohamed, Tarek M

    Biotechnology reports. 2020 Dec., v. 28

    2020  

    Abstract: This study aims to investigate the interactions between osthole extracted from Egyptian citrus fruits as HDACs inhibitor by theoretical study and practically. Besides, osthole was assed as anti-cancer activity. In this study, osthole was extracted from ... ...

    Abstract This study aims to investigate the interactions between osthole extracted from Egyptian citrus fruits as HDACs inhibitor by theoretical study and practically. Besides, osthole was assed as anti-cancer activity. In this study, osthole was extracted from the Egyptian citrus fruit and was characterized. The role of osthole as in vitro inhibitor of HDACs was estimated and evaluated the antitumor activity against human lung cancer cells (A549), Caspase-9 activity was detected. The results obtained from GC–MS indicate that the grapefruit showed the highest osthole concentration compared to the other citrus fruits. Moreover, the grapefruit osthole competitively inhibits HDACs. The inhibition constant value, (Ki=3.36 mM), indicates that osthole exerts an inhibitory effect upon HDACs activity. In vitro study of osthole could inhibit the growth of A549 cells that depend on time and concentration. It also induces apoptosis and causes an increase of caspase-9 by osthole. In conclusion, grapefruit osthole could induce the apoptosis in A549 lung cancer cells by inhibiting the histone deacetylase.
    Keywords Citrus ; antineoplastic activity ; apoptosis ; biotechnology ; caspase-9 ; cell lines ; grapefruits ; histone deacetylase ; histones ; humans ; lung neoplasms
    Language English
    Dates of publication 2020-12
    Publishing place Elsevier B.V.
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 2801018-8
    ISSN 2215-017X
    ISSN 2215-017X
    DOI 10.1016/j.btre.2020.e00531
    Database NAL-Catalogue (AGRICOLA)

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  7. Article ; Online: Functional Assembly of

    Abosharaf, Hamed A / Sakamoto, Yuki / Radwan, Aliaa M / Yuzu, Keisuke / Fujimura, Mika / Diab, Thoria / Mohamed, Tarek M / Chatani, Eri / Kimura, Tetsunari / Tsubaki, Motonari

    Biomolecules

    2021  Volume 11, Issue 1

    Abstract: Among seven homologs of ... ...

    Abstract Among seven homologs of cytochrome
    MeSH term(s) Animals ; Caenorhabditis elegans/metabolism ; Caenorhabditis elegans Proteins/isolation & purification ; Caenorhabditis elegans Proteins/metabolism ; Detergents/pharmacology ; Dynamic Light Scattering ; FMN Reductase/metabolism ; Glucosides/pharmacology ; L-Lactate Dehydrogenase (Cytochrome)/isolation & purification ; L-Lactate Dehydrogenase (Cytochrome)/metabolism ; Lipid Bilayers/metabolism ; Micelles ; Nanoparticles/chemistry ; Particle Size ; Phospholipids/metabolism ; Schiff Bases
    Chemical Substances 2-((p-hydroxylphenylimino)methyl)phenol ; Caenorhabditis elegans Proteins ; Detergents ; Glucosides ; Lipid Bilayers ; Micelles ; Phospholipids ; Schiff Bases ; dodecyl maltoside (69227-93-6) ; L-Lactate Dehydrogenase (Cytochrome) (EC 1.1.2.3) ; FMN Reductase (EC 1.5.1.38) ; ferric citrate iron reductase (EC 1.6.99.-)
    Language English
    Publishing date 2021-01-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2701262-1
    ISSN 2218-273X ; 2218-273X
    ISSN (online) 2218-273X
    ISSN 2218-273X
    DOI 10.3390/biom11010096
    Database MEDical Literature Analysis and Retrieval System OnLINE

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