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  1. AU="Abouelkhair, Mohamed A"
  2. AU="Draia, Ahmed N"
  3. AU="Martins, Paulo"
  4. AU=Elliott P
  5. AU="Gill, J L"
  6. AU="Marmé, Dieter"
  7. AU=St John Ashley L
  8. AU="Macpherson, Catherine Fiona"
  9. AU=Malloy Giovanni S P
  10. AU="Bovino, Antonio"
  11. AU="Deseri, Luca"
  12. AU="Cunningham, C W"
  13. AU="Haas, Brian"
  14. AU="Raia, Anais"
  15. AU=Gollin Susanne M
  16. AU="Xie, Hong-Guang"
  17. AU="Ford, Paul Leicester"
  18. AU="Garver-Daniels, N. E."
  19. AU="De Pisapia, Nicola"
  20. AU="Inoue, Kazunori"
  21. AU="Tüzün, Funda"
  22. AU="McDonough, John"
  23. AU="Puche-Cañas, Emilio"
  24. AU="Rahim, Faraan O"
  25. AU="Barritt, Andrew W"

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  1. Artikel ; Online: Whole Genome Sequencing and Comparative Genomics of Six

    Abouelkhair, Mohamed A / Kania, Stephen A

    Genes

    2024  Band 15, Heft 3

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus schleiferi
    Mesh-Begriff(e) Animals ; Dogs ; Staphylococcal Skin Infections/drug therapy ; Staphylococcal Skin Infections/microbiology ; Anti-Bacterial Agents/therapeutic use ; Dog Diseases ; Staphylococcus/genetics ; Genomics ; Whole Genome Sequencing
    Chemische Substanzen Anti-Bacterial Agents
    Sprache Englisch
    Erscheinungsdatum 2024-02-24
    Erscheinungsland Switzerland
    Dokumenttyp Journal Article
    ZDB-ID 2527218-4
    ISSN 2073-4425 ; 2073-4425
    ISSN (online) 2073-4425
    ISSN 2073-4425
    DOI 10.3390/genes15030284
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  2. Artikel: Non-SARS-CoV-2 genome sequences identified in clinical samples from COVID-19 infected patients: Evidence for co-infections.

    Abouelkhair, Mohamed A

    PeerJ

    2020  Band 8, Seite(n) e10246

    Abstract: Background: In December 2019, an ongoing outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/ 2019-nCoV) infection was initially reported in Wuhan, Hubei Province, China. Early in 2020, the World Health ... ...

    Abstract Background: In December 2019, an ongoing outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/ 2019-nCoV) infection was initially reported in Wuhan, Hubei Province, China. Early in 2020, the World Health Organization (WHO) announced a new name for the 2019-nCoV-caused disease: coronavirus disease 2019 (COVID-19) and declared COVID-19 to be a Public Health Emergency of International Concern (PHEIC). Cellular co-infection is a critical determinant of viral fitness and infection outcomes and plays a crucial role in shaping the host immune response to infections.
    Methods: In this study, 68 public next-generation sequencing data from SARS-CoV-2 infected patients were retrieved from the NCBI Sequence Read Archive database using SRA-Toolkit. Data screening was performed using an alignment-free method based on
    Results: SARS-CoV-2 was identified in all except three patients. Influenza type A (H7N9) virus, human immunodeficiency virus, rhabdovirus, human metapneumovirus, Human adenovirus, Human herpesvirus 1, coronavirus NL63, parvovirus, simian virus 40, and hepatitis virus genomes sequences were detected in SARS-CoV-2 infected patients. Besides, a very diverse group of bacterial populations were observed in the samples.
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-11-02
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 2703241-3
    ISSN 2167-8359
    ISSN 2167-8359
    DOI 10.7717/peerj.10246
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  3. Artikel ; Online: Targeting adenosinergic pathway and adenosine A

    Abouelkhair, Mohamed A

    Medical hypotheses

    2020  Band 144, Seite(n) 110012

    Abstract: The most serious health issue today is the rapid outbreak of Coronavirus Disease 2019 (COVID-19). More than 6,973,427 confirmed cases were diagnosed in nearly 213 countries and territories around the world and two international conveyances, causing ... ...

    Abstract The most serious health issue today is the rapid outbreak of Coronavirus Disease 2019 (COVID-19). More than 6,973,427 confirmed cases were diagnosed in nearly 213 countries and territories around the world and two international conveyances, causing globally over 400,000 deaths. Epidemiology, risk factors, and clinical characteristics of COVID-19 patients have been identified, but the factors influencing the immune system against COVID-19 have not been well established. Upon infection or cell damage, high amounts of adenosine triphosphate (ATP) are released from damaged cells, which serve as mediators of inflammation through purinergic cell surface receptor signaling. As a protective mechanism to prevent excessive damage to host tissue, adenosine counteracts ATP's effects by adenosine receptor stimulation to suppress the pro-inflammatory response. Adenosine is seen as a major obstacle to the efficacy of immune therapies, and the adenosinergic axis components are critical therapeutic targets for cancer and microbial infections. Pharmacologic inhibitors or antibodies specific to adenosinergic pathway components or adenosine receptors in microbial and tumor therapy have shown efficacy in pre-clinical studies and are entering the clinical arena. In this review, we provide a novel hypothesis explaining the potential for improving the efficiency of innate and adaptive immune systems by targeting adenosinergic pathway components and adenosine A
    Mesh-Begriff(e) 5'-Nucleotidase/metabolism ; Adaptive Immunity/drug effects ; Adenosine A2 Receptor Antagonists/pharmacology ; Adenosine A2 Receptor Antagonists/therapeutic use ; Adenosine Triphosphate/metabolism ; Apyrase/metabolism ; COVID-19/drug therapy ; COVID-19/epidemiology ; COVID-19/immunology ; COVID-19/metabolism ; GPI-Linked Proteins/metabolism ; Humans ; Immunity, Innate/drug effects ; Interferon-beta/physiology ; Models, Immunological ; Molecular Targeted Therapy ; Pandemics ; Receptor, Adenosine A2A/physiology ; SARS Virus/immunology ; SARS-CoV-2/immunology ; Signal Transduction/drug effects ; Virus Replication/drug effects
    Chemische Substanzen Adenosine A2 Receptor Antagonists ; GPI-Linked Proteins ; Receptor, Adenosine A2A ; Interferon-beta (77238-31-4) ; Adenosine Triphosphate (8L70Q75FXE) ; 5'-Nucleotidase (EC 3.1.3.5) ; NT5E protein, human (EC 3.1.3.5) ; Apyrase (EC 3.6.1.5) ; ENTPD1 protein, human (EC 3.6.1.5)
    Schlagwörter covid19
    Sprache Englisch
    Erscheinungsdatum 2020-06-19
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2020.110012
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel ; Online: Molecular characterization of a reptarenavirus detected in a Colombian Red-Tailed Boa (Boa constrictor imperator).

    Abouelkhair, Mohamed A / Roozitalab, Ashkan / Elsakhawy, Ola K

    Virology journal

    2023  Band 20, Heft 1, Seite(n) 265

    Abstract: The global decline in biodiversity is a matter of great concern for members of the class Reptilia. Reptarenaviruses infect snakes, and have been linked to various clinical conditions, such as Boid Inclusion Body Disease (BIBD) in snakes belonging to the ... ...

    Abstract The global decline in biodiversity is a matter of great concern for members of the class Reptilia. Reptarenaviruses infect snakes, and have been linked to various clinical conditions, such as Boid Inclusion Body Disease (BIBD) in snakes belonging to the families Boidae and Pythonidae. However, there is a scarcity of information regarding reptarenaviruses found in snakes in both the United States and globally. This study aimed to contribute to the understanding of reptarenavirus diversity by molecularly characterizing a reptarenavirus detected in a Colombian Red-Tailed Boa (Boa constrictor imperator). Using a metagenomics approach, we successfully identified, and de novo assembled the whole genomic sequences of a reptarenavirus in a Colombian Red-Tailed Boa manifesting clinically relevant symptoms consistent with BIBD. The analysis showed that the Colombian Red-Tailed Boa in this study carried the University of Giessen virus (UGV-1) S or S6 (UGV/S6) segment and L genotype 7. The prevalence of the UGV/S6 genotype, in line with prior research findings, implies that this genotype may possess specific advantageous characteristics or adaptations that give it a competitive edge over other genotypes in the host population. This research underscores the importance of monitoring and characterizing viral pathogens in captive and wild snake populations. Knowledge of such viruses is crucial for the development of effective diagnostic methods, potential intervention strategies, and the conservation of vulnerable reptilian species. Additionally, our study provides valuable insights for future studies focusing on the evolutionary history, molecular epidemiology, and biological properties of reptarenaviruses in boas and other snake species.
    Mesh-Begriff(e) Humans ; Animals ; Arenaviridae/genetics ; Boidae ; Colombia ; Biological Evolution ; Genotype
    Sprache Englisch
    Erscheinungsdatum 2023-11-15
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 2160640-7
    ISSN 1743-422X ; 1743-422X
    ISSN (online) 1743-422X
    ISSN 1743-422X
    DOI 10.1186/s12985-023-02237-2
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  5. Artikel ; Online: Complete coding sequence of two feline panleukopenia virus strains isolated from domestic cats (

    Roozitalab, Ashkan / Elsakhawy, Ola K / Abouelkhair, Mohamed A

    Microbiology resource announcements

    2023  Band 12, Heft 10, Seite(n) e0043123

    Abstract: Here, we announce the complete coding sequence of two strains of feline panleukopenia virus (FPLV) that were obtained from deceased domestic cats in animal shelters in Tennessee. The provided sequence data will contribute to a deeper comprehension of the ...

    Abstract Here, we announce the complete coding sequence of two strains of feline panleukopenia virus (FPLV) that were obtained from deceased domestic cats in animal shelters in Tennessee. The provided sequence data will contribute to a deeper comprehension of the genetic characteristics and evolutionary patterns of FPLV in the USA.
    Sprache Englisch
    Erscheinungsdatum 2023-09-28
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/MRA.00431-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  6. Artikel: Non-SARS-CoV-2 genome sequences identified in clinical samples from COVID-19 infected patients: Evidence for co-infections

    Abouelkhair, Mohamed A.

    PeerJ

    Abstract: Background In December 2019, an ongoing outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/ 2019-nCoV) infection was initially reported in Wuhan, Hubei Province, China Early in 2020, the World Health Organization ( ...

    Abstract Background In December 2019, an ongoing outbreak of pneumonia caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2/ 2019-nCoV) infection was initially reported in Wuhan, Hubei Province, China Early in 2020, the World Health Organization (WHO) announced a new name for the 2019-nCoV-caused disease: coronavirus disease 2019 (COVID-19) and declared COVID-19 to be a Public Health Emergency of International Concern (PHEIC) Cellular co-infection is a critical determinant of viral fitness and infection outcomes and plays a crucial role in shaping the host immune response to infections Methods In this study, 68 public next-generation sequencing data from SARS-CoV-2 infected patients were retrieved from the NCBI Sequence Read Archive database using SRA-Toolkit Data screening was performed using an alignment-free method based on k-mer mapping and extension, fastv Taxonomic classification was performed using Kraken 2 on all reads containing one or more virus sequences other than SARS-CoV-2 Results SARS-CoV-2 was identified in all except three patients Influenza type A (H7N9) virus, human immunodeficiency virus, rhabdovirus, human metapneumovirus, Human adenovirus, Human herpesvirus 1, coronavirus NL63, parvovirus, simian virus 40, and hepatitis virus genomes sequences were detected in SARS-CoV-2 infected patients Besides, a very diverse group of bacterial populations were observed in the samples
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #902925
    Datenquelle COVID19

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  7. Artikel: Targeting adenosinergic pathway and adenosine A2A receptor signaling for the treatment of COVID-19: A hypothesis

    Abouelkhair, Mohamed A

    Med Hypotheses

    Abstract: The most serious health issue today is the rapid outbreak of Coronavirus Disease 2019 (COVID-19). More than 6,973,427 confirmed cases were diagnosed in nearly 213 countries and territories around the world and two international conveyances, causing ... ...

    Abstract The most serious health issue today is the rapid outbreak of Coronavirus Disease 2019 (COVID-19). More than 6,973,427 confirmed cases were diagnosed in nearly 213 countries and territories around the world and two international conveyances, causing globally over 400,000 deaths. Epidemiology, risk factors, and clinical characteristics of COVID-19 patients have been identified, but the factors influencing the immune system against COVID-19 have not been well established. Upon infection or cell damage, high amounts of adenosine triphosphate (ATP) are released from damaged cells, which serve as mediators of inflammation through purinergic cell surface receptor signaling. As a protective mechanism to prevent excessive damage to host tissue, adenosine counteracts ATP's effects by adenosine receptor stimulation to suppress the pro-inflammatory response. Adenosine is seen as a major obstacle to the efficacy of immune therapies, and the adenosinergic axis components are critical therapeutic targets for cancer and microbial infections. Pharmacologic inhibitors or antibodies specific to adenosinergic pathway components or adenosine receptors in microbial and tumor therapy have shown efficacy in pre-clinical studies and are entering the clinical arena. In this review, we provide a novel hypothesis explaining the potential for improving the efficiency of innate and adaptive immune systems by targeting adenosinergic pathway components and adenosine A2A receptor signaling for the treatment of COVID-19.
    Schlagwörter covid19
    Verlag WHO
    Dokumenttyp Artikel
    Anmerkung WHO #Covidence: #32590324
    Datenquelle COVID19

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  8. Artikel ; Online: Targeting adenosinergic pathway and adenosine A2A receptor signaling for the treatment of COVID-19

    Abouelkhair, Mohamed A.

    Medical Hypotheses

    A hypothesis

    2020  Band 144, Seite(n) 110012

    Schlagwörter General Medicine ; covid19
    Sprache Englisch
    Verlag Elsevier BV
    Erscheinungsland us
    Dokumenttyp Artikel ; Online
    ZDB-ID 193145-3
    ISSN 1532-2777 ; 0306-9877
    ISSN (online) 1532-2777
    ISSN 0306-9877
    DOI 10.1016/j.mehy.2020.110012
    Datenquelle BASE - Bielefeld Academic Search Engine (Lebenswissenschaftliche Auswahl)

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  9. Artikel ; Online: Complete Genome Sequences of 11 Staphylococcus pseudintermedius Isolates from Dogs in the United States.

    Roozitalab, Ashkan / Elsakhawy, Ola / Phophi, Lufuno / Kania, Stephen A / Abouelkhair, Mohamed A

    Microbiology resource announcements

    2023  Band 12, Heft 4, Seite(n) e0000223

    Abstract: We report here the genome sequences of 11 canine Staphylococcus pseudintermedius isolates from New York, New Hampshire, California, Pennsylvania, and Kansas. The sequencing information will enable spatial phylogenetic comparisons of staphylococcal ... ...

    Abstract We report here the genome sequences of 11 canine Staphylococcus pseudintermedius isolates from New York, New Hampshire, California, Pennsylvania, and Kansas. The sequencing information will enable spatial phylogenetic comparisons of staphylococcal species and other related species and will help in better understanding their virulence potential.
    Sprache Englisch
    Erscheinungsdatum 2023-03-06
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ISSN 2576-098X
    ISSN (online) 2576-098X
    DOI 10.1128/mra.00002-23
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel ; Online: Temporal changes in antibiotic resistance and population structure of methicillin-resistant Staphylococcus pseudintermedius between 2010 and 2021 in the United States.

    Phophi, Lufuno / Abouelkhair, Mohamed A / Jones, Rebekah / Zehr, Jordan / Kania, Stephen A

    Comparative immunology, microbiology and infectious diseases

    2023  Band 100, Seite(n) 102028

    Abstract: The aim of this study was to perform a phenotypic and molecular epidemiological survey to determine temporal changes in the antimicrobial resistance and population structure of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in the United ... ...

    Abstract The aim of this study was to perform a phenotypic and molecular epidemiological survey to determine temporal changes in the antimicrobial resistance and population structure of methicillin-resistant Staphylococcus pseudintermedius (MRSP) in the United States. Samples from 200 S. pseudintermedius isolates were obtained from veterinary diagnostic facilities located in geographic regions sampled approximately ten years ago and compared to samples obtained in 2021. Kirby-Bauer disk diffusion was used to determine antimicrobial susceptibility. geoBURST analysis and MrBayes were used to infer relationships of isolates using MLST data. Almost all MRSP isolates (98%) in 2021 were multidrug-resistant with 21% of these isolates resistant to more than 16 antimicrobials. In 2010, 190 S. pseudintermedius isolates were collected and 141 of them were MRSP. From 2010-2021 there was a significant increase in resistance observed to all antibiotics tested except cephalothin and sulfonamides. Whereas ten years previously multilocus sequence types (ST) ST68 (35.7%), ST71 (10%), and ST84 (17.4%) predominated, these strains have been supplanted by other STs, notably ST45 (n = 14), ST155 (n = 9), ST181 (n = 13), ST496 (n = 9) and ST551 (n = 9). The newly prevalent STs are distantly related to ST68, ST71 and ST84 and most likely do not share any recent common ancestors. The population structure of MRSP is far more elastic than expected with new, highly resistant strains replacing the ones that predominated in the United States a decade ago. Antibiotic use may play a role in selection; however, the strains that were replaced were also multidrug-resistant and other factors are likely involved.
    Mesh-Begriff(e) Dogs ; United States/epidemiology ; Animals ; Methicillin-Resistant Staphylococcus aureus ; Methicillin Resistance ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/veterinary ; Multilocus Sequence Typing/veterinary ; Dog Diseases/epidemiology ; Anti-Bacterial Agents/pharmacology ; Anti-Infective Agents ; Microbial Sensitivity Tests/veterinary
    Chemische Substanzen ST68 ; Anti-Bacterial Agents ; Anti-Infective Agents
    Sprache Englisch
    Erscheinungsdatum 2023-07-24
    Erscheinungsland England
    Dokumenttyp Journal Article
    ZDB-ID 436522-7
    ISSN 1878-1667 ; 0147-9571
    ISSN (online) 1878-1667
    ISSN 0147-9571
    DOI 10.1016/j.cimid.2023.102028
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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