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  1. Article ; Online: Rapid single-photon color imaging of moving objects.

    Yao, Dan / Connolly, Peter W R / Sykes, Arran J / Shah, Yash D / Accarino, Claudio / Grant, James / Cumming, David R S / Buller, Gerald S / McLaughlin, Stephen / Altmann, Yoann

    Optics express

    2023  Volume 31, Issue 16, Page(s) 26610–26625

    Abstract: This paper outlines an experimental demonstration of a Bayesian image reconstruction approach to achieve rapid single-photon color imaging of moving objects. The capacity to extract the color of objects is important in a variety of target identification ... ...

    Abstract This paper outlines an experimental demonstration of a Bayesian image reconstruction approach to achieve rapid single-photon color imaging of moving objects. The capacity to extract the color of objects is important in a variety of target identification and computer vision applications. Nonetheless, it remains challenging to achieve high-speed color imaging of moving objects in low-photon flux environments. The low-photon regime presents particular challenges for efficient spectral separation and identification, while unsupervised image reconstruction algorithms are often slow and computationally expensive. In this paper, we address both of these difficulties using a combination of hardware and computational solutions. We demonstrate color imaging using a Single-Photon Avalanche Diode (SPAD) detector array for rapid, low-light-level data acquisition, with an integrated color filter array (CFA) for efficient spectral unmixing. High-speed image reconstruction is achieved using a bespoke Bayesian algorithm to produce high-fidelity color videos. The analysis is conducted first on simulated data allowing different pixel formats and photon flux scenarios to be investigated. Experiments are then performed using a plasmonic metasurface-based CFA, integrated with a 64 × 64 pixel format SPAD array. Passive imaging is conducted using white-light illumination of multi-colored, moving targets. Intensity information is recorded in a series of 2D photon-counting SPAD frames, from which accurate color information is extracted using the fast Bayesian method introduced herein. The per-frame reconstruction rate proves to be hundreds of times faster than the previous computational method. Furthermore, this approach yields additional information in the form of uncertainty measures, which can be used to assist with imaging system optimization and decision-making in real-world applications. The techniques demonstrated point the way towards rapid video-rate single-photon color imaging. The developed Bayesian algorithm, along with more advanced SPAD technology and utilization of time-correlated single-photon counting (TCSPC) will permit live 3D, color videography in extremely low-photon flux environments.
    Language English
    Publishing date 2023-09-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1491859-6
    ISSN 1094-4087 ; 1094-4087
    ISSN (online) 1094-4087
    ISSN 1094-4087
    DOI 10.1364/OE.493172
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Simultaneous multi-spectral, single-photon fluorescence imaging using a plasmonic colour filter array.

    Connolly, Peter W R / Valli, Jessica / Shah, Yash D / Altmann, Yoann / Grant, James / Accarino, Claudio / Rickman, Colin / Cumming, David R S / Buller, Gerald S

    Journal of biophotonics

    2021  Volume 14, Issue 7, Page(s) e202000505

    Abstract: We present the first realisation of simultaneous multi-spectral fluorescence imaging using a single-photon avalanche diode (SPAD) array, where the spectral unmixing is facilitated by a plasmonic metasurface mosaic colour filter array (CFA). A 64 × 64 ... ...

    Abstract We present the first realisation of simultaneous multi-spectral fluorescence imaging using a single-photon avalanche diode (SPAD) array, where the spectral unmixing is facilitated by a plasmonic metasurface mosaic colour filter array (CFA). A 64 × 64 pixel format silicon SPAD array is used to record widefield fluorescence and brightfield data from four biological samples. A plasmonic metasurface composed of an arrangement of circular and elliptical nanoholes etched into an aluminium thin film deposited on a glass substrate provides the high transmission efficiency CFA, enabling a bespoke spectral unmixing algorithm to reconstruct high fidelity, full colour images from as few as ∼3 photons per pixel. This approach points the way toward real-time, single-photon sensitive multi-spectral fluorescence imaging. Furthermore, this is possible without additional bulky components such as a filter wheel, prism or diffraction grating, nor the need for multiple sample exposures or multiple detectors.
    MeSH term(s) Algorithms ; Color ; Microscopy, Fluorescence ; Optical Imaging ; Photons
    Language English
    Publishing date 2021-05-04
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2390063-5
    ISSN 1864-0648 ; 1864-063X
    ISSN (online) 1864-0648
    ISSN 1864-063X
    DOI 10.1002/jbio.202000505
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Multimodal Integrated Sensor Platform for Rapid Biomarker Detection.

    Al-Rawhani, Mohammed A / Hu, Chunxiao / Giagkoulovits, Christos / Annese, Valerio F / Cheah, Boon Chong / Beeley, James / Velugotla, Srinivas / Accarino, Claudio / Grant, James P / Mitra, Srinjoy / Barrett, Michael P / Cochran, Sandy / Cumming, David R S

    IEEE transactions on bio-medical engineering

    2019  Volume 67, Issue 2, Page(s) 614–623

    Abstract: Precision metabolomics and quantification for cost-effective rapid diagnosis of disease are the key goals in personalized medicine and point-of-care testing. At present, patients are subjected to multiple test procedures requiring large laboratory ... ...

    Abstract Precision metabolomics and quantification for cost-effective rapid diagnosis of disease are the key goals in personalized medicine and point-of-care testing. At present, patients are subjected to multiple test procedures requiring large laboratory equipment. Microelectronics has already made modern computing and communications possible by integration of complex functions within a single chip. As More than Moore technology increases in importance, integrated circuits for densely patterned sensor chips have grown in significance. Here, we present a versatile single complementary metal-oxide-semiconductor chip forming a platform to address personalized needs through on-chip multimodal optical and electrochemical detection that will reduce the number of tests that patients must take. The chip integrates interleaved sensing subsystems for quadruple-mode colorimetric, chemiluminescent, surface plasmon resonance, and hydrogen ion measurements. These subsystems include a photodiode array and a single photon avalanche diode array with some elements functionalized to introduce a surface plasmon resonance mode. The chip also includes an array of ion sensitive field-effect transistors. The sensor arrays are distributed uniformly over an active area on the chip surface in a scalable and modular design. Bio-functionalization of the physical sensors yields a highly selective simultaneous multiple-assay platform in a disposable format. We demonstrate its versatile capabilities through quantified bio-assays performed on-chip for glucose, cholesterol, urea, and urate, each within their naturally occurring physiological range.
    MeSH term(s) Biomarkers/analysis ; Biosensing Techniques/instrumentation ; Blood Glucose/analysis ; Chemistry Techniques, Analytical/instrumentation ; Cholesterol/blood ; Equipment Design ; Humans ; Nanotechnology/instrumentation ; Semiconductors ; Uric Acid/analysis
    Chemical Substances Biomarkers ; Blood Glucose ; Uric Acid (268B43MJ25) ; Cholesterol (97C5T2UQ7J)
    Language English
    Publishing date 2019-06-19
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 160429-6
    ISSN 1558-2531 ; 0018-9294
    ISSN (online) 1558-2531
    ISSN 0018-9294
    DOI 10.1109/TBME.2019.2919192
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: A monolithic single-chip point-of-care platform for metabolomic prostate cancer detection.

    Annese, Valerio F / Patil, Samadhan B / Hu, Chunxiao / Giagkoulovits, Christos / Al-Rawhani, Mohammed A / Grant, James / Macleod, Martin / Clayton, David J / Heaney, Liam M / Daly, Ronan / Accarino, Claudio / Shah, Yash D / Cheah, Boon C / Beeley, James / Evans, Thomas R Jeffry / Jones, Robert / Barrett, Michael P / Cumming, David R S

    Microsystems & nanoengineering

    2021  Volume 7, Page(s) 21

    Abstract: There is a global unmet need for rapid and cost-effective prognostic and diagnostic tools that can be used at the bedside or in the doctor's office to reduce the impact of serious disease. Many cancers are diagnosed late, leading to costly treatment and ... ...

    Abstract There is a global unmet need for rapid and cost-effective prognostic and diagnostic tools that can be used at the bedside or in the doctor's office to reduce the impact of serious disease. Many cancers are diagnosed late, leading to costly treatment and reduced life expectancy. With prostate cancer, the absence of a reliable test has inhibited the adoption of screening programs. We report a microelectronic point-of-care metabolite biomarker measurement platform and use it for prostate cancer detection. The platform, using an array of photodetectors configured to operate with targeted, multiplexed, colorimetric assays confined in monolithically integrated passive microfluidic channels, completes a combined assay of 4 metabolites in a drop of human plasma in under 2 min. A preliminary clinical study using l-amino acids, glutamate, choline, and sarcosine was used to train a cross-validated random forest algorithm. The system demonstrated sensitivity to prostate cancer of 94% with a specificity of 70% and an area under the curve of 0.78. The technology can implement many similar assay panels and hence has the potential to revolutionize low-cost, rapid, point-of-care testing.
    Language English
    Publishing date 2021-03-05
    Publishing country England
    Document type Journal Article
    ISSN 2055-7434
    ISSN (online) 2055-7434
    DOI 10.1038/s41378-021-00243-4
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article ; Online: An integrated portable system for single chip simultaneous measurement of multiple disease associated metabolites.

    Patil, Samadhan B / Dheeman, Dharmendra S / Al-Rawhani, Mohammed A / Velugotla, Srinivas / Nagy, Bence / Cheah, Boon Chong / Grant, James P / Accarino, Claudio / Barrett, Michael P / Cumming, David R S

    Biosensors & bioelectronics

    2018  Volume 122, Page(s) 88–94

    Abstract: Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously ... ...

    Abstract Metabolites, the small molecules that underpin life, can act as indicators of the physiological state of the body when their abundance varies, offering routes to diagnosis of many diseases. The ability to assay for multiple metabolites simultaneously will underpin a new generation of precision diagnostic tools. Here, we report the development of a handheld device based on complementary metal oxide semiconductor (CMOS) technology with multiple isolated micro-well reaction zones and integrated optical sensing allowing simultaneous enzyme-based assays of multiple metabolites (choline, xanthine, sarcosine and cholesterol) associated with multiple diseases. These metabolites were measured in clinically relevant concentration range with minimum concentrations measured: 25 μM for choline, 100 μM for xanthine, 1.25 μM for sarcosine and 50 μM for cholesterol. Linking the device to an Android-based user interface allows for quantification of metabolites in serum and urine within 2 min of applying samples to the device. The quantitative performance of the device was validated by comparison to accredited tests for cholesterol and glucose.
    MeSH term(s) Biosensing Techniques/instrumentation ; Cholesterol/blood ; Cholesterol/urine ; Choline/blood ; Choline/urine ; Equipment Design ; Humans ; Lab-On-A-Chip Devices ; Male ; Oxides/chemistry ; Point-of-Care Systems ; Sarcosine/blood ; Sarcosine/urine ; Semiconductors ; Xanthine/blood ; Xanthine/urine
    Chemical Substances Oxides ; Xanthine (1AVZ07U9S7) ; Cholesterol (97C5T2UQ7J) ; Choline (N91BDP6H0X) ; Sarcosine (Z711V88R5F)
    Language English
    Publishing date 2018-09-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 1011023-9
    ISSN 1873-4235 ; 0956-5663
    ISSN (online) 1873-4235
    ISSN 0956-5663
    DOI 10.1016/j.bios.2018.09.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

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