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  1. Article: Seroprevalence of Mycobacteriumavium subsp. paratuberculosis in Dairy Cattle in Khartoum State, Sudan

    Elmagzoub, Wisal A / Adam, Nabawia M / Idris, Sanaa M / Mukhtar, Mohamed E / Abdelaziz, Sanaa A / Okuni, Julius B / Ojok, Lonzy / Abd El Wahed, Ahmed / Eltayeb, ElSagad / Gameel, Ahmed A / Eltom, Kamal H

    Veterinary sciences. 2020 Dec. 21, v. 7, no. 4

    2020  

    Abstract: Paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic wasting disease mainly of domestic and wild ruminants. It occurs worldwide, causing significant economic losses through decreased productivity, low fertility, ...

    Abstract Paratuberculosis, caused by Mycobacterium avium subspecies paratuberculosis (MAP), is a chronic wasting disease mainly of domestic and wild ruminants. It occurs worldwide, causing significant economic losses through decreased productivity, low fertility, increased cull rates and mortality. It is listed by the OIE (World Organization for Animal Health) as a disease of concern to trade in animals. Prevalence of this disease can be studied by detecting anti-MAP antibodies by Enzyme linked immunosorbent Assay (ELISA). The aim of this study was to investigate the current prevalence of MAP infection in cattle in Khartoum State. The overall apparent prevalence of MAP infection was found to be 6.3% and 18.9% at animal and herd levels, respectively. All seropositive animals were cross-bred females of good body condition; most of them (>90%) were >3 years old and >50% were from medium-sized herds in Omdurman. No significant association (p > 0.05) was found between seropositivity and animal herd size. The prevalence of MAP infection in Khartoum State is still low to medium compared to other parts of the world, but it is comparable to those reported from other African countries. Further studies with the view of designing nationwide surveys in domestic ruminants and camels in other states of the country are needed for establishing control programmes.
    Keywords Mycobacterium avium subsp. paratuberculosis ; World Organization for Animal Health ; body condition ; crossbreds ; dairy cattle ; enzymes ; herd size ; mortality ; paratuberculosis ; seroprevalence ; Sudan
    Language English
    Dates of publication 2020-1221
    Publishing place Multidisciplinary Digital Publishing Institute
    Document type Article
    Note NAL-light
    ZDB-ID 2768971-2
    ISSN 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci7040209
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Seroprevalence of

    Elmagzoub, Wisal A / Adam, Nabawia M / Idris, Sanaa M / Mukhtar, Mohamed E / Abdelaziz, Sanaa A / Okuni, Julius B / Ojok, Lonzy / Abd El Wahed, Ahmed / Eltayeb, ElSagad / Gameel, Ahmed A / Eltom, Kamal H

    Veterinary sciences

    2020  Volume 7, Issue 4

    Abstract: Paratuberculosis, caused ... ...

    Abstract Paratuberculosis, caused by
    Language English
    Publishing date 2020-12-21
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2768971-2
    ISSN 2306-7381 ; 2306-7381
    ISSN (online) 2306-7381
    ISSN 2306-7381
    DOI 10.3390/vetsci7040209
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Differentiation of Capripox Viruses by Nanopore Sequencing.

    Eltom, Kamal H / Althoff, Anna Christina / Hansen, Sören / Böhlken-Fascher, Susanne / Yousif, Ausama / El-Sheikh, Hussein A / ElWakeel, Ahmed A / Elgamal, Mahmoud A / Mossa, Hadeer M / Aboul-Soud, Emad A / Wolff, Janika / Korthase, Christian / Hoffmann, Bernd / Adam, Nabawia M / Abdelaziz, Sanaa A / Shalaby, Mohamed A / Abd El Wahed, Ahmed

    Vaccines

    2021  Volume 9, Issue 4

    Abstract: The genus capripoxvirus (CaPV), ... ...

    Abstract The genus capripoxvirus (CaPV), family
    Language English
    Publishing date 2021-04-06
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2703319-3
    ISSN 2076-393X
    ISSN 2076-393X
    DOI 10.3390/vaccines9040351
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Differentiation of Capripox Viruses by Nanopore Sequencing

    Eltom, Kamal H. / Althoff, Anna Christina / Hansen, Sören / Böhlken-Fascher, Susanne / Yousif, Ausama / El-Sheikh, Hussein A. / ElWakeel, Ahmed A. / Elgamal, Mahmoud A. / Mossa, Hadeer M. / Aboul-Soud, Emad A. / Wolff, Janika / Korthase, Christian / Hoffmann, Bernd / Adam, Nabawia M. / Abdelaziz, Sanaa A. / Shalaby, Mohamed A. / Abd El Wahed, Ahmed

    2021  

    Abstract: The genus capripoxvirus (CaPV), family Poxviridae, includes three virus species: goatpox virus (GPV), sheeppox virus (SPV) and lumpy skin disease virus (LSDV). CaPV causes disease outbreaks with consequent economic losses in Africa and the Middle East. ... ...

    Abstract The genus capripoxvirus (CaPV), family Poxviridae, includes three virus species: goatpox virus (GPV), sheeppox virus (SPV) and lumpy skin disease virus (LSDV). CaPV causes disease outbreaks with consequent economic losses in Africa and the Middle East. LSDV has recently spread to Southeast Europe. As CaPVs share 96–97% genetic similarity along the length of the entire genome and are difficult to distinguish using serological assays, simple, reliable and fast methods for diagnosis and species differentiation are crucial in cases of disease outbreak. The present study aimed to develop a field-applicable CaPV differentiation method. Nanopore technology was used for whole genome sequencing. A local database of complete CaPV genomes and partial sequences of three genes (RPO30, P32 and GPCR) was established for offline Basic Local Alignment Search Tool (BLAST). Specificities of 98.04% in whole genome and 97.86% in RPO30 gene runs were obtained among the three virus species, while other databases were less specific. The total run time was shortened to approximately 2 h. Functionality of the developed procedure was proved by samples with high host background sequences. Reliable differentiation options for the quality and capacity of hardware, and sample quality of suspected cases, were derived from these findings. The whole workflow can be performed rapidly with a mobile suitcase laboratory and mini-computer, allowing application at the point-of-need with limited resource settings.
    Keywords Text ; ddc:630 ; lumpy skin disease -- capripox virus -- nanopore sequencing
    Language English
    Publishing date 2021-04-06
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  5. Book ; Online: Differentiation of Capripox Viruses by Nanopore Sequencing

    Eltom, Kamal H. / Althoff, Anna Christina / Hansen, Sören / Böhlken-Fascher, Susanne / Yousif, Ausama / El-Sheikh, Hussein A. / ElWakeel, Ahmed A. / Elgamal, Mahmoud A. / Mossa, Hadeer. M. / Aboul-Soud, Emad A. / Wolff, Janika / Korthase, Christian / Hoffmann, Bernd / Adam, Nabawia M. / Abdelaziz, Sanaa A. / Shalaby, Mohamed A. / Abd El Wahed, Ahmed

    2021  

    Abstract: Original BLAST data base and Nanopore sequences data of the identification of capripox viruses. ...

    Abstract Original BLAST data base and Nanopore sequences data of the identification of capripox viruses.
    Keywords Text ; ddc:570 ; Capripox Viruses -- BLAST data base -- Nanopore sequence data
    Language English
    Publishing date 2021-02-24
    Publisher Zenodo
    Publishing country de
    Document type Book ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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