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  1. Article ; Online: Development of Cas13a-based assays for

    Allan-Blitz, Lao-Tzu / Shah, Palak / Adams, Gordon / Branda, John A / Klausner, Jeffrey D / Goldstein, Robert / Sabeti, Pardis C / Lemieux, Jacob E

    mSphere

    2023  Volume 8, Issue 5, Page(s) e0041623

    Abstract: Neisseria ... ...

    Abstract Neisseria gonorrhoeae
    MeSH term(s) Infant, Newborn ; Humans ; Neisseria gonorrhoeae/genetics ; Gonorrhea/diagnosis ; Gonorrhea/microbiology ; HIV Infections ; Drug Resistance, Bacterial/genetics ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Ciprofloxacin/pharmacology
    Chemical Substances Anti-Bacterial Agents ; Ciprofloxacin (5E8K9I0O4U)
    Language English
    Publishing date 2023-09-21
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ISSN 2379-5042
    ISSN (online) 2379-5042
    DOI 10.1128/msphere.00416-23
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Development of Cas13a-based Assays for

    Allan-Blitz, Lao-Tzu / Shah, Palak / Adams, Gordon / Branda, John A / Klausner, Jeffrey D / Goldstein, Robert / Sabeti, Pardis C / Lemieux, Jacob E

    medRxiv : the preprint server for health sciences

    2023  

    Abstract: Background: Neisseria gonorrhoeae: Methods and results: We designed and optimized RNA guides and primer-sets for SHERLOCK assays capable of detecting : Conclusion: We report the development of Cas13a-based SHERLOCK assays that ... ...

    Abstract Background: Neisseria gonorrhoeae
    Methods and results: We designed and optimized RNA guides and primer-sets for SHERLOCK assays capable of detecting
    Conclusion: We report the development of Cas13a-based SHERLOCK assays that detect
    Language English
    Publishing date 2023-05-26
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2023.05.21.23290304
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Babesia microti

    Holbrook, Nolan R / Klontz, Erik H / Adams, Gordon C / Schnittman, Samuel R / Issa, Nicolas C / Bond, Sheila A / Branda, John A / Lemieux, Jacob E

    Open forum infectious diseases

    2023  Volume 10, Issue 3, Page(s) ofad097

    Abstract: ... We ... ...

    Abstract We report
    Language English
    Publishing date 2023-02-23
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2757767-3
    ISSN 2328-8957
    ISSN 2328-8957
    DOI 10.1093/ofid/ofad097
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Clinical Performance of Cas13a-based Point-of-Care Lateral Flow Assay for Detecting

    Allan-Blitz, Lao-Tzu / Sanders, Gabriela / Shah, Palak / Adams, Gordon / Jarolimova, Jana / Ard, Kevin / Branda, John A / Klausner, Jeffrey D / Sabeti, Pardis C / Lemieux, Jacob E

    medRxiv : the preprint server for health sciences

    2024  

    Abstract: Background: Diagnosis of : Methods: We collected an additional urine specimen among individuals enrolling in an ongoing clinical trial at the Massachusetts General Hospital Sexual Health Clinic who presented with symptoms of urethritis or cervicitis ( ...

    Abstract Background: Diagnosis of
    Methods: We collected an additional urine specimen among individuals enrolling in an ongoing clinical trial at the Massachusetts General Hospital Sexual Health Clinic who presented with symptoms of urethritis or cervicitis (urethral or vaginal discharge, dysuria, or dyspareunia). We then assessed thermal, detergent, and combination DNA extraction conditions, varying the duration of heat at 95°C and concentration of Triton X. We assessed the efficacy of the various DNA extraction methods by quantitative polymerase chain reaction (qPCR). Once an extraction method was selected, we incubated samples for 90 minutes to permit isothermal recombinase polymerase amplification. We then assessed the performance of lateral flow Cas13a-based detection using our previously designed
    Results: We assessed DNA extraction conditions on 3 clinical urine specimens. There was no consistent significant difference in copies per microliter of DNA obtained using more or less heat. On average, we noted that 0.02% triton combined with 5 minutes of heating to 95°C resulted in the highest DNA yield, however, 0.02% triton alone resulted in a quantity of DNA that was above the previously determined analytic sensitivity of the assay. Given that detergent-based extraction is more easily deployable, we selected that as our method for extraction. We treated 23 clinical specimens with 0.02% triton, which we added to the Cas13a detection system. We ran all lateral flow detections in duplicate. The Cas13a-based assay detected 8 of 8 (100%) positive specimens, and 0 of 15 negative specimens.
    Conclusion: Using point-of-care DNA extraction, isothermal amplification, and Cas13a-based detection, our point-of-care lateral flow
    Language English
    Publishing date 2024-03-04
    Publishing country United States
    Document type Preprint
    DOI 10.1101/2024.03.01.24303603
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Book: Mission creep

    Adams, Gordon / Murray, Shoon Kathleen

    the militarization of US foreign policy?

    2014  

    Institution United States / Department of Defense
    United States / Department of State
    Author's details Gordon Adams and Shoon Murray ed
    Keywords Civil-military relations ; Interagency coordination ; United States/Military policy ; United States
    Language English
    Size XII, 303 S., graph. Darst.
    Publisher Georgetown Univ. Press
    Publishing place Washington, DC
    Document type Book
    Note Includes bibliographical references and index
    ISBN 9781626160934 ; 9781626160941 ; 9781626161146 ; 1626160937 ; 1626160945 ; 1626161143
    Database Former special subject collection: coastal and deep sea fishing

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  6. Book ; Conference proceedings: Fast processes in radiation chemistry and biology

    Adams, Gordon Ernest

    proceedings of the Fifth L. H. Gray Conference, held at the University of Sussex 10 - 14 September 1973

    1975  

    Institution University of Sussex
    Event/congress L. H. Gray Conference (5, 1973, Brighton)
    Author's details ed. by G. E. Adams
    Language English
    Size IX, 381 S. : Ill., graph. Darst.
    Publisher Inst. of Physics u.a.
    Publishing place Bristol
    Publishing country Great Britain
    Document type Book ; Conference proceedings
    HBZ-ID HT005161726
    ISBN 0-471-00475-8 ; 978-0-471-00475-2
    Database Catalogue ZB MED Medicine, Health

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Shelf markLoan statusLocation
    1976 A 959 order for loan Magazin

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  7. Book ; Online: Mission Creep

    Adams, Gordon / Murray, Shoon

    The Militarization of US Foreign Policy

    2014  

    Abstract: Mission Creep: The Militarization of US Foreign Policy? examines the question of whether the US Department of Defense (DOD) has assumed too large a role in influencing and implementing US foreign policy. After the Cold War, and accelerating after ... ...

    Abstract Mission Creep: The Militarization of US Foreign Policy? examines the question of whether the US Department of Defense (DOD) has assumed too large a role in influencing and implementing US foreign policy. After the Cold War, and accelerating after September 11, the United States has drawn upon the enormous resources of DOD in adjusting to the new global environment and challenges arising from terrorism, Islamic radicalism, insurgencies, ethnic conflicts, and failed states. Contributors investigate and provide different perspectives on the extent to which military leaders and DOD have increased
    Language English
    Size Online-Ressource (318 p)
    Publisher Georgetown University Press
    Publishing place Washington
    Document type Book ; Online
    Note Description based upon print version of record
    ISBN 9781626160934 ; 1626160937
    Database Library catalogue of the German National Library of Science and Technology (TIB), Hannover

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  8. Article ; Online: Single-cell profiling of lncRNA expression during Ebola virus infection in rhesus macaques.

    Santus, Luisa / Sopena-Rios, Maria / García-Pérez, Raquel / Lin, Aaron E / Adams, Gordon C / Barnes, Kayla G / Siddle, Katherine J / Wohl, Shirlee / Reverter, Ferran / Rinn, John L / Bennett, Richard S / Hensley, Lisa E / Sabeti, Pardis C / Melé, Marta

    Nature communications

    2023  Volume 14, Issue 1, Page(s) 3866

    Abstract: Long non-coding RNAs (lncRNAs) are involved in numerous biological processes and are pivotal mediators of the immune response, yet little is known about their properties at the single-cell level. Here, we generate a multi-tissue bulk RNAseq dataset from ... ...

    Abstract Long non-coding RNAs (lncRNAs) are involved in numerous biological processes and are pivotal mediators of the immune response, yet little is known about their properties at the single-cell level. Here, we generate a multi-tissue bulk RNAseq dataset from Ebola virus (EBOV) infected and not-infected rhesus macaques and identified 3979 novel lncRNAs. To profile lncRNA expression dynamics in immune circulating single-cells during EBOV infection, we design a metric, Upsilon, to estimate cell-type specificity. Our analysis reveals that lncRNAs are expressed in fewer cells than protein-coding genes, but they are not expressed at lower levels nor are they more cell-type specific when expressed in the same number of cells. In addition, we observe that lncRNAs exhibit similar changes in expression patterns to those of protein-coding genes during EBOV infection, and are often co-expressed with known immune regulators. A few lncRNAs change expression specifically upon EBOV entry in the cell. This study sheds light on the differential features of lncRNAs and protein-coding genes and paves the way for future single-cell lncRNA studies.
    MeSH term(s) Animals ; Hemorrhagic Fever, Ebola/genetics ; RNA, Long Noncoding/genetics ; Macaca mulatta ; Ebolavirus/genetics ; Virus Internalization
    Chemical Substances RNA, Long Noncoding
    Language English
    Publishing date 2023-06-30
    Publishing country England
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2553671-0
    ISSN 2041-1723 ; 2041-1723
    ISSN (online) 2041-1723
    ISSN 2041-1723
    DOI 10.1038/s41467-023-39627-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Book: Europe's defence industry

    Adams, Gordon

    a transatlantic future?

    1999  

    Institution Centre for European Reform
    Author's details [Publ. by the Centre for European Reform]. Gordon Adams
    Language English
    Size 69 S
    Publisher CER
    Publishing place London
    Document type Book
    ISBN 1901229122 ; 9781901229127
    Database Former special subject collection: coastal and deep sea fishing

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  10. Book: Europe's defence industry

    Adams, Gordon

    a transatlantic future?

    1999  

    Institution Centre for European Reform
    Author's details [Publ. by the Centre for European Reform]. Gordon Adams
    Language English
    Size 69 S
    Publisher CER
    Publishing place London
    Document type Book
    ISBN 1901229122 ; 9781901229127
    Database ECONomics Information System

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