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  1. Article ; Online: Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics

    Adolfo, Laci M. / Rao, Xiaolan / Dixon, Richard A.

    BMC Plant Biol. 2022 Dec., v. 22, no. 1 p.10-10

    2022  

    Abstract: BACKGROUND: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of ... ...

    Abstract BACKGROUND: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature. RESULTS: We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity. CONCLUSIONS: The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.
    Keywords DNA barcoding ; Oriental traditional medicine ; Pueraria montana var. lobata ; biosynthesis ; daidzein ; gene expression ; genetic analysis ; internal transcribed spacers ; microsatellite repeats ; noxious weeds ; species identification ; transcriptome ; transcriptomics
    Language English
    Dates of publication 2022-12
    Size p. 10.
    Publishing place BioMed Central
    Document type Article ; Online
    ZDB-ID 2059868-3
    ISSN 1471-2229
    ISSN 1471-2229
    DOI 10.1186/s12870-021-03383-x
    Database NAL-Catalogue (AGRICOLA)

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  2. Article ; Online: Identification of Pueraria spp. through DNA barcoding and comparative transcriptomics.

    Adolfo, Laci M / Rao, Xiaolan / Dixon, Richard A

    BMC plant biology

    2022  Volume 22, Issue 1, Page(s) 10

    Abstract: Background: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of ...

    Abstract Background: Kudzu is a term used generically to describe members of the genus Pueraria. Kudzu roots have been used for centuries in traditional Chinese medicine in view of their high levels of beneficial isoflavones including the unique 8-C-glycoside of daidzein, puerarin. In the US, kudzu is seen as a noxious weed causing ecological and economic damage. However, not all kudzu species make puerarin or are equally invasive. Kudzu remains difficult to identify due to its diverse morphology and inconsistent nomenclature.
    Results: We have generated sequences for the internal transcribed spacer 2 (ITS2) and maturase K (matK) regions of Pueraria montana lobata, P. montana montana, and P. phaseoloides, and identified two accessions previously used for differential analysis of puerarin biosynthesis as P. lobata and P. phaseoloides. Additionally, we have generated root transcriptomes for the puerarin-producing P. m. lobata and the non-puerarin producing P. phaseoloides. Within the transcriptomes, microsatellites were identified to aid in species identification as well as population diversity.
    Conclusions: The barcode sequences generated will aid in fast and efficient identification of the three kudzu species. Additionally, the microsatellites identified from the transcriptomes will aid in genetic analysis. The root transcriptomes also provide a molecular toolkit for comparative gene expression analysis towards elucidation of the biosynthesis of kudzu phytochemicals.
    MeSH term(s) DNA Barcoding, Taxonomic ; Gene Expression Profiling ; Isoflavones/analysis ; Plant Roots/chemistry ; Plant Weeds/classification ; Plant Weeds/genetics ; Pueraria/classification ; Pueraria/genetics ; Transcriptome
    Chemical Substances Isoflavones ; puerarin (Z9W8997416)
    Language English
    Publishing date 2022-01-03
    Publishing country England
    Document type Journal Article
    ISSN 1471-2229
    ISSN (online) 1471-2229
    DOI 10.1186/s12870-021-03383-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Evaluation of pathways to the C‐glycosyl isoflavone puerarin in roots of kudzu (Pueraria montana lobata)

    Adolfo, Laci M. / Burks, David / Rao, Xiaolan / Alvarez‐Hernandez, Anislay / Dixon, Richard A.

    Plant direct. 2022 Sept., v. 6, no. 9

    2022  

    Abstract: Kudzu (Pueraria montana lobata) is used as a traditional medicine in China and Southeast Asia but is a noxious weed in the Southeastern United States. It produces both O‐ and C‐glycosylated isoflavones, with puerarin (C‐glucosyl daidzein) as an important ...

    Abstract Kudzu (Pueraria montana lobata) is used as a traditional medicine in China and Southeast Asia but is a noxious weed in the Southeastern United States. It produces both O‐ and C‐glycosylated isoflavones, with puerarin (C‐glucosyl daidzein) as an important bioactive compound. Currently, the stage of the isoflavone pathway at which the C‐glycosyl unit is added remains unclear, with a recent report of direct C‐glycosylation of daidzein contradicting earlier labeling studies supporting C‐glycosylation at the level of chalcone. We have employed comparative mRNA sequencing of the roots from two Pueraria species, one of which produces puerarin (field collected P. montana lobata) and one of which does not (commercial Pueraria phaseoloides), to identify candidate uridine diphosphate glycosyltransferase (UGT) enzymes involved in puerarin biosynthesis. Expression of recombinant UGTs in Escherichia coli and candidate C‐glycosyltransferases in Medicago truncatula were used to explore substrate specificities, and gene silencing of UGT and key isoflavone biosynthetic genes in kudzu hairy roots employed to test hypotheses concerning the substrate(s) for C‐glycosylation. Our results confirm UGT71T5 as a C‐glycosyltransferase of isoflavone biosynthesis in kudzu. Enzymatic, isotope labeling, and genetic analyses suggest that puerarin arises both from the direct action of UGT71T5 on daidzein and via a second route in which the C‐glycosidic linkage is introduced to the chalcone isoliquiritigenin.
    Keywords Escherichia coli ; Medicago truncatula ; Pueraria montana var. lobata ; Pueraria phaseoloides ; bioactive compounds ; biosynthesis ; chalcone ; daidzein ; genes ; isotopes ; noxious weeds ; traditional medicine ; uridine diphosphate ; China ; South East Asia
    Language English
    Dates of publication 2022-09
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ISSN 2475-4455
    DOI 10.1002/pld3.442
    Database NAL-Catalogue (AGRICOLA)

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    Inter-library loan at ZB MED

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  4. Article ; Online: Evaluation of pathways to the

    Adolfo, Laci M / Burks, David / Rao, Xiaolan / Alvarez-Hernandez, Anislay / Dixon, Richard A

    Plant direct

    2022  Volume 6, Issue 9, Page(s) e442

    Abstract: Kudzu ( ...

    Abstract Kudzu (
    Language English
    Publishing date 2022-09-02
    Publishing country England
    Document type Journal Article
    ISSN 2475-4455
    ISSN (online) 2475-4455
    DOI 10.1002/pld3.442
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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