LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 4 of total 4

Search options

  1. Article ; Online: Transcriptome-wide-scale-predicted dsRNAs potentially involved in RNA homoeostasis are remarkably excluded from genes with no/very low expression in all developmental stages.

    Pasquier, Claude / Agnel, Sandra / Robichon, Alain

    RNA biology

    2020  Volume 17, Issue 4, Page(s) 554–570

    Abstract: RNA interference (RNAi) refers to a conserved posttranscriptional mechanism for the degradation of RNA by short dsRNAs. A genome-wide analysis of mRNAs that are complementary to RNAs of variable length that are transcribed from the full transcriptome and ...

    Abstract RNA interference (RNAi) refers to a conserved posttranscriptional mechanism for the degradation of RNA by short dsRNAs. A genome-wide analysis of mRNAs that are complementary to RNAs of variable length that are transcribed from the full transcriptome and susceptible to being loaded onto
    MeSH term(s) Animals ; Argonaute Proteins/metabolism ; Computational Biology/methods ; Drosophila Proteins/genetics ; Drosophila Proteins/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/growth & development ; Exons ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Introns ; RNA Helicases/metabolism ; RNA, Double-Stranded/metabolism ; RNA, Long Noncoding/genetics ; RNA, Messenger/genetics ; Ribonuclease III/metabolism ; Whole Exome Sequencing
    Chemical Substances AGO2 protein, Drosophila ; Argonaute Proteins ; Drosophila Proteins ; RNA, Double-Stranded ; RNA, Long Noncoding ; RNA, Messenger ; DCR-2 protein, Drosophila (EC 2.7.7.-) ; Ribonuclease III (EC 3.1.26.3) ; RNA Helicases (EC 3.6.4.13)
    Language English
    Publishing date 2020-02-04
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ISSN 1555-8584
    ISSN (online) 1555-8584
    DOI 10.1080/15476286.2020.1717154
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article: Transcriptome-wide-scale-predicted dsRNAs potentially involved in RNA homoeostasis are remarkably excluded from genes with no/very low expression in all developmental stages

    Pasquier, Claude / Agnel, Sandra / Robichon, Alain

    RNA biology. 2020 Apr. 2, v. 17, no. 4

    2020  

    Abstract: RNA interference (RNAi) refers to a conserved posttranscriptional mechanism for the degradation of RNA by short dsRNAs. A genome-wide analysis of mRNAs that are complementary to RNAs of variable length that are transcribed from the full transcriptome and ...

    Abstract RNA interference (RNAi) refers to a conserved posttranscriptional mechanism for the degradation of RNA by short dsRNAs. A genome-wide analysis of mRNAs that are complementary to RNAs of variable length that are transcribed from the full transcriptome and susceptible to being loaded onto Argonaute type 2 was performed through computational searches in the Drosophila model. We report the segments of RNAs that are complementary to mRNAs originating from introns, the exons of mRNAs and lncRNAs as a potential source of siRNAs. A full catalogue of the mRNAs that fulfill these criteria is presented, along with the quantification of multiple annealing. The catalogue was assessed for biological validation using three published lists: two for Ago2-associated RNAs and one for dsRNAs isolated from a crude extract. A broad spectrum of mRNAs were found to theoretically form intermolecular segmental dsRNAs, which should qualify them as Dicer/Ago2 substrates if they exist in vivo. These results suggest a genome-wide scale of mRNA homoeostasis via RNAi metabolism and could extend the known roles of canonical miRNAs and hairpin RNAs. The distribution of the genes for which transcripts are engaged in intermolecular segmental pairing is largely lacking in the gene collections defined as showing no expression in each individual developmental stage from early embryos to adulthood. This trend was also observed for the genes showing very low expression from the 8-12-hour embryonic to larval stage 2. This situation was also suggested by the 3 lists generated with minimal 20-, 25- and 30-base pairing lengths.
    Keywords Drosophila ; RNA interference ; double-stranded RNA ; exons ; genome-wide association study ; introns ; larvae ; messenger RNA ; metabolism ; microRNA ; models ; nucleic acid annealing ; small interfering RNA ; transcription (genetics) ; transcriptome
    Language English
    Dates of publication 2020-0402
    Size p. 554-570.
    Publishing place Taylor & Francis
    Document type Article
    ISSN 1555-8584
    DOI 10.1080/15476286.2020.1717154
    Database NAL-Catalogue (AGRICOLA)

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: The Mapping of Predicted Triplex DNA:RNA in the

    Pasquier, Claude / Agnel, Sandra / Robichon, Alain

    G3 (Bethesda, Md.)

    2017  Volume 7, Issue 7, Page(s) 2295–2304

    Abstract: Double-stranded DNA is able to form triple-helical structures by accommodating a third nucleotide strand. A nucleic acid triplex occurs according to Hoogsteen rules that predict the stability and affinity of the third strand bound to the Watson-Crick ... ...

    Abstract Double-stranded DNA is able to form triple-helical structures by accommodating a third nucleotide strand. A nucleic acid triplex occurs according to Hoogsteen rules that predict the stability and affinity of the third strand bound to the Watson-Crick duplex. The "triplex-forming oligonucleotide" (TFO) can be a short sequence of RNA that binds to the major groove of the targeted duplex only when this duplex presents a sequence of purine or pyrimidine bases in one of the DNA strands. Many nuclear proteins are known to bind triplex DNA or DNA:RNA, but their biological functions are unexplored. We identified sequences that are capable of engaging as the "triplex-forming oligonucleotide" in both the pre-lncRNA and pre-mRNA collections of
    MeSH term(s) Animals ; DNA/genetics ; Drosophila melanogaster ; Gene Regulatory Networks ; Genes, Insect ; Morphogenesis/genetics ; Nucleic Acid Heteroduplexes ; RNA
    Chemical Substances Nucleic Acid Heteroduplexes ; RNA (63231-63-0) ; DNA (9007-49-2)
    Language English
    Publishing date 2017--05
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2629978-1
    ISSN 2160-1836 ; 2160-1836
    ISSN (online) 2160-1836
    ISSN 2160-1836
    DOI 10.1534/g3.117.042911
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article ; Online: Transcriptome Profiling of Neurosensory Perception Genes in Wing Tissue of Two Evolutionary Distant Insect Orders: Diptera (Drosophila melanogaster) and Hemiptera (Acyrthosiphon pisum).

    Agnel, Sandra / da Rocha, Martine / Robichon, Alain

    Journal of molecular evolution

    2017  Volume 85, Issue 5-6, Page(s) 234–245

    Abstract: The neurogenesis and neuronal functions in insect wing have been understudied mainly due to technical hindrances that have prevented electrophysiology studies for decades. The reason is that the nano-architecture of the wing chemosensory bristles hampers ...

    Abstract The neurogenesis and neuronal functions in insect wing have been understudied mainly due to technical hindrances that have prevented electrophysiology studies for decades. The reason is that the nano-architecture of the wing chemosensory bristles hampers the receptors accessibility of odorants/tastants to receptors in fixed setup, whereas in nature, the wing flapping mixes these molecules in bristle lymph. In this report, we analyzed the transcriptome of the wing tissue of two species phylogenetically strongly divergent: Drosophila melanogaster a generic model for diptera order (complete metamorphosis) and the aphid acyrthosiphon pisum, representative of hemiptera order (incomplete metamorphosis) for which a conditional winged/wingless polyphenism is under control of population density and resources. The transcriptome shows that extensive gene networks involved in chemosensory perception are active in adult wing for both species. Surprisingly, the specific transcripts of genes that are commonly found in eye were present in Drosophila wing but not in aphid. The analysis reveals that in the aphid conditional wing, expressed genes show strong similarities with those in the gut epithelia. This suggests that the epithelial cell layer between the cuticle sheets is persistent at least in young aphid adult, whereas it disappears after emergence in Drosophila. Despite marked differences between the two transcriptomes, the results highlight the probable universalism of wing chemosensory function in the holometabolous and hemimetabolous orders of winged insects.
    MeSH term(s) Animals ; Aphids/genetics ; Aphids/metabolism ; Biological Evolution ; Chemoreceptor Cells/metabolism ; Drosophila melanogaster/genetics ; Drosophila melanogaster/metabolism ; Gene Expression Profiling/methods ; Gene Regulatory Networks ; Genes, Insect/genetics ; Hemiptera/genetics ; Insecta/genetics ; Neurons ; Phylogeny ; Transcriptome ; Wings, Animal/anatomy & histology ; Wings, Animal/chemistry ; Wings, Animal/metabolism
    Language English
    Publishing date 2017-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 120148-7
    ISSN 1432-1432 ; 0022-2844
    ISSN (online) 1432-1432
    ISSN 0022-2844
    DOI 10.1007/s00239-017-9814-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 1129: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top