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  1. Article ; Online: Engineering a nanoantibiotic system displaying dual mechanism of action.

    Xing, Huihua / de Campos, Luana Janaína / Pereira, Aramis Jose / Fiora, Maria Mercedes / Aguiar-Alves, Fabio / Tagliazucchi, Mario / Conda-Sheridan, Martin

    Proceedings of the National Academy of Sciences of the United States of America

    2024  Volume 121, Issue 16, Page(s) e2321498121

    Abstract: In recent decades, peptide amphiphiles (PAs) have established themselves as promising self-assembling bioinspired materials in a wide range of medical fields. Herein, we report a dual-therapeutic system constituted by an antimicrobial PA and a ... ...

    Abstract In recent decades, peptide amphiphiles (PAs) have established themselves as promising self-assembling bioinspired materials in a wide range of medical fields. Herein, we report a dual-therapeutic system constituted by an antimicrobial PA and a cylindrical protease inhibitor (LJC) to achieve broad antimicrobial spectrum and to enhance therapeutic efficacy. We studied two strategies: PA-LJC nanostructures (
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Nanostructures/chemistry ; Anti-Infective Agents
    Chemical Substances Anti-Bacterial Agents ; Anti-Infective Agents
    Language English
    Publishing date 2024-04-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 209104-5
    ISSN 1091-6490 ; 0027-8424
    ISSN (online) 1091-6490
    ISSN 0027-8424
    DOI 10.1073/pnas.2321498121
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Evaluation of changes in antimicrobial susceptibility in bacteria infecting children and their mothers in pediatric, neonatal-intensive care unit, and gynecology/obstetrics wards of a quaternary referral hospital during the COVID-19 pandemic.

    Pinheiro, Felipe Ramos / Rozza-de-Menezes, Rafaela Elvira / Blum, Marina Camille / Pereira, Renata Freire Alves / Rocha, Jaqueline Abel / Guedes Pinto, Maria Cristina F / Penna, Bruno A / Riley, Lee W / Aguiar-Alves, Fabio

    Frontiers in microbiology

    2023  Volume 14, Page(s) 1096223

    Abstract: The World Health Organization released a statement warning of increased risk for the incidence of multidrug resistant microorganisms and the absence of new drugs to control such infections soon. Since the beginning of the COVID-19 pandemic, the ... ...

    Abstract The World Health Organization released a statement warning of increased risk for the incidence of multidrug resistant microorganisms and the absence of new drugs to control such infections soon. Since the beginning of the COVID-19 pandemic, the prescription of antimicrobial agents has increased and may have accelerated the emergence of multidrug resistant (MDR) bacteria. This study aimed to evaluate maternal and pediatric infections within a hospital from January 2019 to December 2021. An observational retrospective cohort study was performed at a quaternary referral hospital in a metropolitan area of Niteroi city, Rio de Janeiro state, Brazil. A total of 196 patients' medical records were analyzed. The data from 90 (45.9%) patients were collected before the SARS-CoV-2 pandemic, 29 (14.8%) from the 2020 pandemic period, and 77 (39.3%) from the 2021 pandemic period. A total of 256 microorganisms were identified during this period. Out of those, 101 (39.5%) were isolated in 2019, 51 (19.9%) in 2020, and 104 (40.6%) in 2021. Antimicrobial susceptibility tests were performed on 196 (76.6%) clinical isolates. The exact binomial test showed that the distribution of Gram-negative bacteria was predominant. The most common microorganism was
    Language English
    Publishing date 2023-02-20
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2023.1096223
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: ‘Hypericum brasiliense: bactericidal and antibiofilm activity against Staphylococcus aureus in vitro’

    Pereira, Renata Freire Alves / Andre, Lialyz Soares Pereira / Pinheiro, Felipe Ramos / Scaffo, Julia / Corrêa, Arthur Luiz / Ribeiro, Tainara Aparecida Nunes / Sachs, Daniela / Rocha, Leandro / Aguiar-Alves, Fabio

    Natural product research. 2022 May 23, v. 36, no. 10

    2022  

    Abstract: Staphylococcus aureus is considered the most common opportunistic pathogen in humans, capable of forming biofilm, increasing the chances of antibiotic resistance and causes several chronic diseases. Biodiversity is a source of inspiration in the search ... ...

    Abstract Staphylococcus aureus is considered the most common opportunistic pathogen in humans, capable of forming biofilm, increasing the chances of antibiotic resistance and causes several chronic diseases. Biodiversity is a source of inspiration in the search for new agents against these microorganisms. Hitherto, the efficacy of Hypericum sp. extracts as an antibacterial agent has already been demonstrated against Gram-positive and Gram-negative bacteria. In this study, we observed that until 4 µg/mL, the Hypericum brasiliense extract showed bactericidal activity against a clinical multidrug-resistant S. aureus strain (HU25) and also inhibited biofilm formation at 1/2xMIC (confirmed by SEM) and 1/4xMIC. The extract was also proportionally active against 6 h-preformed biofilm to its concentration (1/2xMIC, 1/4xMIC, p value ≤ 0.05). These promising results make Hypericum brasiliense extract a strong candidate to treat S. aureus infections, including anti-biofilm therapy.
    Keywords Hypericum ; antibacterial properties ; antibiotic resistance ; antibiotics ; biodiversity ; biofilm ; multiple drug resistance ; opportunistic pathogens ; research ; therapeutics
    Language English
    Dates of publication 2022-0523
    Size p. 2643-2647.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2021.1915306
    Database NAL-Catalogue (AGRICOLA)

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  4. Article ; Online: First report of meticillin-resistant Staphylococcus aureus harboring mecC gene in milk samples from cows with mastitis in southeastern Brazil.

    Alves, Maria de Fatima N F / Penna, Bruno / Pereira, Renata F A / Geraldo, Reinaldo B / Folly, Evelize / Castro, Helena Carla / Aguiar-Alves, Fabio

    Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology

    2020  Volume 51, Issue 4, Page(s) 2175–2179

    Abstract: MRSA infection and colonization have been reported in both companion and food-chain animals, highlighting MRSA as an important veterinary and zoonotic pathogen. Another mec allele, the mecC gene, also confers beta-lactam resistance in Staphylococcus ... ...

    Abstract MRSA infection and colonization have been reported in both companion and food-chain animals, highlighting MRSA as an important veterinary and zoonotic pathogen. Another mec allele, the mecC gene, also confers beta-lactam resistance in Staphylococcus aureus and shows 69% nucleotide identity to mecA. The main aim of this study was to investigate the genotypic and clonal profile of methicillin-resistant S. aureus (MRSA) from cows with mastitis in dairy herds. Thirty-five samples suggestive of bovine subclinical mastitis were evaluated, and S. aureus were detected in all of them using both phenotypic and molecular approaches. According to the multilocus sequence typing (MLST), the S. aureus isolates were assigned in five different STs, 21 (60%) showed ST 742, 6 (17%) ST97, 4 (11%) ST1, 2 (6%) ST30, and 2 (6%) ST126. The presence of mecA was not observed in any of these isolates whereas mecC was detected in nine of them (9/35; 26%). The Panton-Valentine leukocidin (PVL) genes were detected in a total of 4 isolates. Among the 35 isolates analyzed, 26 showed resistance to penicillin. Changes in the S. aureus epidemiology due to the detection of MRSA in milk samples from cows presenting with bovine subclinical mastitis may have consequences for public health in Brazil, challenging the empirical therapy and animal management, with potential medical and social outcomes. To the best of our knowledge, this is the first report describing mecC MRSA in Southeastern Brazil.
    MeSH term(s) Animals ; Anti-Bacterial Agents ; Bacterial Proteins/genetics ; Bacterial Toxins/genetics ; Bacterial Typing Techniques ; Brazil ; Cattle ; Exotoxins/genetics ; Female ; Genotype ; Leukocidins/genetics ; Mastitis, Bovine/microbiology ; Methicillin-Resistant Staphylococcus aureus/classification ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Microbial Sensitivity Tests ; Milk/microbiology ; Multilocus Sequence Typing ; Staphylococcal Infections/veterinary
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; Bacterial Toxins ; Exotoxins ; Leukocidins ; Panton-Valentine leukocidin
    Language English
    Publishing date 2020-10-21
    Publishing country Brazil
    Document type Journal Article
    ZDB-ID 2017175-4
    ISSN 1678-4405 ; 1517-8382
    ISSN (online) 1678-4405
    ISSN 1517-8382
    DOI 10.1007/s42770-020-00385-z
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Biofilm formation in catheter-related infections by Panton-Valentine leukocidin-producing Staphylococcus aureus.

    Silva-Santana, Giorgio / Lenzi-Almeida, Kátia C / Lopes, Vânia G S / Aguiar-Alves, Fábio

    International microbiology : the official journal of the Spanish Society for Microbiology

    2017  Volume 19, Issue 4, Page(s) 199–207

    Abstract: The use of invasive techniques, such as intravascular catheter insertion, and the formation of biofilms in several devices by methicillin-resistant Staphylococcus aureus (MRSA) have contributed to the increased number of septic patients, morbidity and ... ...

    Abstract The use of invasive techniques, such as intravascular catheter insertion, and the formation of biofilms in several devices by methicillin-resistant Staphylococcus aureus (MRSA) have contributed to the increased number of septic patients, morbidity and mortality. This study aimed to evaluate the virulence of strains through catheter colonization and identification of microbial biofilm, as well as pathological changes on the colonized skin. An experimental biofilm formation model utilized catheter fragments implanted subcutaneously in 25 Swiss mice. The technique consisted of inoculating a catheter fragment on the back of each animal, followed by intradermal inoculation of 50 μl of bacterial suspension at 1.0 × 10⁷ colony forming units/ml. After 96 h, catheters were removed for macroscopic analysis and evaluated through culture. Local skin fragments were also extracted for histopathology analysis. Staphylococcus aureus can adhere to catheters, colonize and form biofilms. The high amount of viable bacterial cells colonizing catheters and virulence factors can lead to severe infections of skin and adjacent tissues. [Int Microbiol 19(4): 199-207 (2016)].
    MeSH term(s) Animals ; Bacterial Toxins/biosynthesis ; Biofilms/growth & development ; Catheter-Related Infections/microbiology ; Exotoxins/biosynthesis ; Humans ; Leukocidins/biosynthesis ; Male ; Methicillin-Resistant Staphylococcus aureus/growth & development ; Methicillin-Resistant Staphylococcus aureus/pathogenicity ; Mice ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/growth & development ; Staphylococcus aureus/pathogenicity
    Chemical Substances Bacterial Toxins ; Exotoxins ; Leukocidins ; Panton-Valentine leukocidin
    Language English
    Publishing date 2017-05-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 1454951-7
    ISSN 1618-1905 ; 1139-6709
    ISSN (online) 1618-1905
    ISSN 1139-6709
    DOI 10.2436/20.1501.01.278
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: '

    Pereira, Renata Freire Alves / Andre, Lialyz Soares Pereira / Pinheiro, Felipe Ramos / Scaffo, Julia / Corrêa, Arthur Luiz / Ribeiro, Tainara Aparecida Nunes / Sachs, Daniela / Rocha, Leandro / Aguiar-Alves, Fabio

    Natural product research

    2021  Volume 36, Issue 10, Page(s) 2643–2647

    Abstract: Staphylococcus ... ...

    Abstract Staphylococcus aureus
    MeSH term(s) Anti-Bacterial Agents/pharmacology ; Biofilms ; Gram-Negative Bacteria ; Gram-Positive Bacteria ; Humans ; Hypericum ; Methicillin-Resistant Staphylococcus aureus ; Microbial Sensitivity Tests ; Plant Extracts/pharmacology ; Staphylococcal Infections ; Staphylococcus aureus
    Chemical Substances Anti-Bacterial Agents ; Plant Extracts
    Language English
    Publishing date 2021-06-02
    Publishing country England
    Document type Journal Article
    ZDB-ID 2185747-7
    ISSN 1478-6427 ; 1478-6419
    ISSN (online) 1478-6427
    ISSN 1478-6419
    DOI 10.1080/14786419.2021.1915306
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  7. Article ; Online: Development and validation of a rabbit model of

    Gras, Emmanuelle / Vu, Trang T T / Nguyen, Nhu T Q / Tran, Vuvi G / Mao, Yanjie / Tran, Nguyen D / Mai, Nam H / Dong, Oliver X / Jung, David H / Iorio, Natalia L P P / Povoa, Helvecio C C / Pinheiro, Marcos Gabriel / Aguiar-Alves, Fabio / Weiss, William J / Zheng, Bo / Cheng, Lily I / Stover, Charles K / Sellman, Bret R / DiGiandomenico, Antonio /
    Gibault, Laure / Valour, Florent / Diep, Binh An

    Frontiers in cellular and infection microbiology

    2023  Volume 13, Page(s) 1297281

    Abstract: Background: New drugs targeting antimicrobial resistant pathogens, including : Methods: We report here an FDA-funded study to develop a rabbit model of non-ventilated pneumonia with : Results: In a rabbit model of non-ventilated pneumonia, ... ...

    Abstract Background: New drugs targeting antimicrobial resistant pathogens, including
    Methods: We report here an FDA-funded study to develop a rabbit model of non-ventilated pneumonia with
    Results: In a rabbit model of non-ventilated pneumonia, endobronchial challenge with live
    Conclusion: The rabbit model may be used to help predict clinical efficacy of new antibacterial drugs for the treatment of non-ventilated
    MeSH term(s) Humans ; Animals ; Rabbits ; Meropenem/therapeutic use ; Pseudomonas aeruginosa ; Microbial Sensitivity Tests ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Pseudomonas Infections/drug therapy ; Pseudomonas Infections/microbiology ; Tobramycin/pharmacology ; Tobramycin/therapeutic use ; Pneumonia/drug therapy ; Drug Development
    Chemical Substances Meropenem (FV9J3JU8B1) ; Anti-Bacterial Agents ; Tobramycin (VZ8RRZ51VK)
    Language English
    Publishing date 2023-12-11
    Publishing country Switzerland
    Document type Journal Article ; Research Support, N.I.H., Extramural
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2023.1297281
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  8. Article ; Online: Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant

    Machado, Thamiris Santana / Pinheiro, Felipe Ramos / Andre, Lialyz Soares Pereira / Pereira, Renata Freire Alves / Correa, Reginaldo Fernandes / de Mello, Gabriela Coutinho / Ribeiro, Tainara Aparecida Nunes / Penna, Bruno / Sachs, Daniela / Aguiar-Alves, Fábio

    Toxins

    2020  Volume 13, Issue 1

    Abstract: Hospitalizations related to Methicillin- ... ...

    Abstract Hospitalizations related to Methicillin-resistant
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Biofilms/growth & development ; Female ; Gene Expression Regulation, Bacterial ; Humans ; Male ; Methicillin Resistance ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Middle Aged ; Nose/microbiology ; Staphylococcal Infections/microbiology ; Virulence Factors/genetics ; Virulence Factors/metabolism ; Young Adult
    Chemical Substances Bacterial Proteins ; Virulence Factors
    Language English
    Publishing date 2020-12-25
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins13010014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  9. Article ; Online: Prevalence of oxacillin-susceptible methicillin-resistant

    Muzy Dias, Aline Pecanha / Guimarães, Lorrayne Cardoso / V Petrucci, Livia B D / Z Pinheiro, Jéssica A / Pinheiro, Marcos Gabriel / Silva, Felipe Rodrigues E / Póvoa, Helvécio C C / Aguiar-Alves, Fábio

    Indian journal of medical microbiology

    2020  Volume 38, Issue 2, Page(s) 192–199

    Abstract: Context: Nosocomial infections arise from many microorganisms, including Staphylococcus aureus.: Aims: The aim of this study is to determine the molecular epidemiology of circulating methicillin-resistant S. aureus (MRSA) clones among patients ... ...

    Abstract Context: Nosocomial infections arise from many microorganisms, including Staphylococcus aureus.
    Aims: The aim of this study is to determine the molecular epidemiology of circulating methicillin-resistant S. aureus (MRSA) clones among patients attending community and health-care facilities in Nova Friburgo, RJ, Brazil.
    Methods: A total of 1002 nasal swab samples were collected from May 2010 to September 2015. S. aureus isolates were identified through phenotypic tests, submitted to antimicrobial susceptibility tests and genotypic analysis to detect mecA, panton-valentine leucocidin (PVL) genes, SCCmec, SPA and multilocus sequencing typing (MLST) typing.
    Results: We identified 294 (29.3%) isolates as S. aureus and 91 (9.1%) as MRSA. A total of 17 isolates did not present a correlation between phenotypic and genotypic resistance profiles. Among MRSA isolates, 17 (18.7%) carried PVL genes. A total of 20 different SPA types were determined, being grouped by MLST into eight different sequence types. ST5/t002 was the most prevalent genotype found among these isolates.
    Conclusions: There is a gradual colonisation shift happening in the infection pattern by S. aureus in Brazil. The Brazilian Epidemic Clone (ST239-SCCmec IIIa-PVL-) seems to be substituted by isolates from different clonal complexes, such as ST5, ST8 and ST30. The non-correlation between phenotypic/genotypic resistance profile observed in some isolates suggests the presence of other methicillin resistance mechanisms different from mecA presence or a difference in the nucleotide sequence, which prevents the primers to identify the specific region during polymerase chain reaction reactions. MRSA identification should be based on phenotypic and genotypic testing to ensure the various types of resistance mechanisms.
    MeSH term(s) Adolescent ; Adult ; Aged ; Aged, 80 and over ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics ; Carrier State/epidemiology ; Carrier State/microbiology ; Cross Infection/epidemiology ; Cross Infection/microbiology ; Cross-Sectional Studies ; Disk Diffusion Antimicrobial Tests ; Drug Resistance, Multiple, Bacterial ; Genes, Bacterial ; Genetic Variation ; Health Facilities ; Humans ; Methicillin-Resistant Staphylococcus aureus/classification ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Middle Aged ; Molecular Epidemiology ; Molecular Typing ; Multilocus Sequence Typing ; Nose/microbiology ; Oxacillin/pharmacology ; Penicillin-Binding Proteins/genetics ; Prevalence ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcal Protein A/genetics ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/isolation & purification ; Young Adult
    Chemical Substances Anti-Bacterial Agents ; Bacterial Proteins ; Penicillin-Binding Proteins ; Staphylococcal Protein A ; mecA protein, Staphylococcus aureus ; Oxacillin (UH95VD7V76)
    Language English
    Publishing date 2020-09-03
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1038798-5
    ISSN 1998-3646 ; 0255-0857
    ISSN (online) 1998-3646
    ISSN 0255-0857
    DOI 10.4103/ijmm.IJMM_20_157
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  10. Article ; Online: Genotypic distribution of Staphylococcus aureus colonizing children and adolescents in daycare centers, an outpatient clinic, and hospitals in a major Brazilian urban setting.

    Neto, Egidio Domingos André / Guerrero, Jaclyn / Snyder, Robert E / Pereira, Renata Freire Alves / de Fátima Nogueira de Freitas, Maria / Silva-Santana, Giorgio / Riley, Lee W / Aguiar-Alves, Fábio

    Diagnostic microbiology and infectious disease

    2020  Volume 97, Issue 3, Page(s) 115058

    Abstract: Staphylococcus aureus nasal colonization is a major risk factor for infection. Studies have suggested an epidemiologic shift in the methicillin-resistant S. aureus (MRSA) strains that circulate in Brazil. We conducted cross-sectional studies of MRSA ... ...

    Abstract Staphylococcus aureus nasal colonization is a major risk factor for infection. Studies have suggested an epidemiologic shift in the methicillin-resistant S. aureus (MRSA) strains that circulate in Brazil. We conducted cross-sectional studies of MRSA carriage among 1) children and adolescents in community daycare centers, 2) an outpatient clinic, and 3) hospitals in a large Brazilian metropolitan setting. There were 1.500 study subjects, 500 from each locale: 768 (51.2%) carried S. aureus whereas 150 (10%) of these were colonized with MRSA. The most common lineages were the Southwest Pacific (SWP) and the Pediatric clones in all three groups. Roughly 50% of SWP carried Panton-Valentine leukocidin (PVL) (p < 0.01) genes while 63.3% of the Pediatric clones were resistant or intermediately resistant to erythromycin (p < 0.01). This study describes a clonal change of the Brazilian epidemic clone (BEC) to the Pediatric and SWP lineages in Brazil. This finding has implications for clinical management of MRSA infections.
    MeSH term(s) Ambulatory Care Facilities ; Anti-Bacterial Agents/pharmacology ; Bacterial Toxins/genetics ; Brazil/epidemiology ; Carrier State ; Child ; Child Day Care Centers ; Child, Preschool ; Cities/epidemiology ; Cross-Sectional Studies ; Exotoxins/genetics ; Female ; Genotype ; Hospitals, Public ; Humans ; Infant ; Leukocidins/genetics ; Male ; Methicillin-Resistant Staphylococcus aureus/drug effects ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Microbial Sensitivity Tests ; Nasal Mucosa/microbiology ; Prevalence ; Staphylococcal Infections/diagnosis ; Staphylococcal Infections/epidemiology ; Staphylococcal Infections/microbiology ; Staphylococcus aureus/drug effects ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification
    Chemical Substances Anti-Bacterial Agents ; Bacterial Toxins ; Exotoxins ; Leukocidins ; Panton-Valentine leukocidin
    Language English
    Publishing date 2020-04-08
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604920-5
    ISSN 1879-0070 ; 0732-8893
    ISSN (online) 1879-0070
    ISSN 0732-8893
    DOI 10.1016/j.diagmicrobio.2020.115058
    Database MEDical Literature Analysis and Retrieval System OnLINE

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