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  1. Article ; Online: Skeletal, dentoalveolar and soft tissue changes after stabilization splint treatment for patients with temporomandibular joint disorders.

    Al-Hadad, Saba Ahmed / Ahmed, Madiha Mohammed Saleh / Zhao, Yunshan / Wang, Lu / Hu, Wanqing / Li, Chushen / Chen, Xi / Alhammadi, Maged Sultan

    BMC oral health

    2024  Volume 24, Issue 1, Page(s) 479

    Abstract: Background: Temporomandibular disorder (TMD) is a grouping of heterogeneous disorders with multifactorial origins. Stabilization splints (SS) have demonstrated an acceptable treatment effect in TMD. The possible changes at the skeletal, dental, and soft ...

    Abstract Background: Temporomandibular disorder (TMD) is a grouping of heterogeneous disorders with multifactorial origins. Stabilization splints (SS) have demonstrated an acceptable treatment effect in TMD. The possible changes at the skeletal, dental, and soft tissue levels need to be addressed to evaluate the benefit/risk ratio of this therapeutic procedure. Accordingly, this study aimed to three‑dimensionally evaluate skeletal, dentoalveolar and soft tissue changes after SS treatment for patients with TMD.
    Methods: This retrospective study included 74 adult patients with myofascial and/or intra-articular disorders (25 males and 49 females), with an average age of 22.88 ± 4.8 years, who underwent SS treatment. Pre- and post-treatment Cone beam computed tomography were analysed using Invivo 6.0.3 software. The primary outcome was the vertical skeletal and dentoalveolar changes, while the secondary outcomes were the anteroposterior skeletal, dentoalveolar and soft tissue changes. Paired t-test and Wilcoxon rank sum test were used for statistical analyses.
    Results: For the primary outcome; skeletally, there was a significant increase in mandibular plane inclination (difference: 0.82°±1.37), decrease facial height ratio (difference: 0.45%±1.07) and at the dentoalveolar level, the inclination of the functional (FOP-SN, FOP-FH) and bisecting (BOP-SN, BOP-FH) occlusal planes exhibited a significant increase too (difference: 0.38 ± 1.43°, 0.49 ± 1.62°, 0.44 ± 1.29° and 0.41 ± 1.17°, respectively) and also a decrease in the overbite (difference: -0.54 ± 0.83). For the secondary outcomes; there was a significant decrease in mandibular position (SNB) (difference: 1.60 ± 1.36°) and increase in the overjet (difference: 0.93 ± 1.04, p < 0.001) and a significant lower lip retrusion (difference: 0.33 ± 1.01 mm p < 0.01), was observed too.
    Conclusions: SS therapy resulted in significant vertical skeletal and dentoalveolar changes that were manifested mainly by facial height ratio, mandibular and occlusal plane changes, and to a lesser extent, significant anteroposterior skeletal, dentoalveolar, and soft tissue changes in the form of mandibular position, increased overjet and a more retrusive lower lip. These changes should be considered during patients' selection prior to initiating SS therapy.
    MeSH term(s) Male ; Adult ; Female ; Humans ; Adolescent ; Young Adult ; Maxilla ; Retrospective Studies ; Splints ; Cephalometry/methods ; Mandible/diagnostic imaging ; Overbite/therapy ; Malocclusion, Angle Class II/therapy ; Temporomandibular Joint Disorders/diagnostic imaging ; Temporomandibular Joint Disorders/therapy ; Temporomandibular Joint
    Language English
    Publishing date 2024-04-20
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091511-1
    ISSN 1472-6831 ; 1472-6831
    ISSN (online) 1472-6831
    ISSN 1472-6831
    DOI 10.1186/s12903-024-04260-3
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Quantitative and qualitative condylar changes following stabilization splint therapy in patients with temporomandibular joint disorders.

    Musa, Mazen / Zhang, Qianqian / Awad, Riham / Wang, Wenfang / Ahmed, Madiha Mohammed Saleh / Zhao, Yunshan / Almashraqi, Abeer A / Chen, Xi / Alhammadi, Maged S

    Clinical oral investigations

    2023  Volume 27, Issue 5, Page(s) 2299–2310

    Abstract: Objective: This study aimed to explore the quantitative and qualitative condylar changes following stabilization splint (S.S) therapy, including condylar position, morphology, and bone mineral density (BMD) in subjects with temporomandibular disorders ( ... ...

    Abstract Objective: This study aimed to explore the quantitative and qualitative condylar changes following stabilization splint (S.S) therapy, including condylar position, morphology, and bone mineral density (BMD) in subjects with temporomandibular disorders (TMD).
    Materials and methods: In this retrospective clinical study, we enrolled 40 TMD subjects (80 joints) aged 18 to 35 years, for whom a S.S was used to treat TMD. The 80 TMD consists of 32 masticatory muscle disorders (myalgia) and 48 TMJ disorders (arthralgia). Cone beam computed tomography (CBCT) was used to scan the TMJs of subjects pre- and post-treatment for three-dimensional analysis (3D). Using Mimics software v.21.0, quantitative (3D condylar and joint spaces dimensions parameters were measured using linear measurements in millimeters, according to the Kamelchuk method and Ikeda method, while the assessment of anteroposterior condyle position within the glenoid fossa was based on the method of Pullinger and Hollender), and qualitative (a round bone tissue with an area of 2 mm
    Results: The course of treatment was 6-12 months, with an average of 9.1 months. For the pre- and post-treatment quantitative comparisons, there was a statistically significant difference in the anterior joint space (AJS) and coronal medial space, as well as the condyle length in the myalgia group and condylar width in the arthralgia group. For qualitative measurements, a significant difference was observed in the posterior slope of the myalgia group and the arthralgia group's anterior, superior, and posterior slopes. The inter-group comparisons revealed significant differences in AJS, condylar length, and anterior slope density.
    Conclusion: In short-term follow-up, the S.S influenced patients with TMD from different origins; it changes anterior and coronal medial joint space, condyle length in myalgia, and width in arthralgia. Furthermore, it improved the condyle bone density more evidently in arthralgia.
    Clinical relevance: This study highlights the influence of S.S on symptomatic populations with TMD of different origins from a qualitative and quantitative perspective.
    MeSH term(s) Humans ; Temporomandibular Joint ; Mandibular Condyle/diagnostic imaging ; Splints ; Myalgia ; Retrospective Studies ; Temporomandibular Joint Disorders/diagnostic imaging ; Temporomandibular Joint Disorders/therapy ; Cone-Beam Computed Tomography/methods ; Arthralgia
    Language English
    Publishing date 2023-04-11
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1364490-7
    ISSN 1436-3771 ; 1432-6981
    ISSN (online) 1436-3771
    ISSN 1432-6981
    DOI 10.1007/s00784-023-04963-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Quantitative and qualitative condylar changes following stabilization splint therapy in patients with temporomandibular joint disorders with and without skeletal lateral mandibular asymmetry: a cone beam computed tomographic study.

    Musa, Mazen / Awad, Riham / Izeldin, Salma / Zhao, Yunshan / Wu, Hao / Wang, Lu / Al-Hadad, Saba Ahmed / Saif, Bdr Sultan / Ahmed, Madiha Mohammed Saleh / Chen, Xi

    BMC oral health

    2024  Volume 24, Issue 1, Page(s) 363

    Abstract: Background: Temporomandibular disorders (TMDs) encompass pain and dysfunction in the jaw, muscles, and adjacent structures. This study aimed to explore the quantitative (condylar position, morphology) and qualitative (bone mineral density (BMD)) ... ...

    Abstract Background: Temporomandibular disorders (TMDs) encompass pain and dysfunction in the jaw, muscles, and adjacent structures. This study aimed to explore the quantitative (condylar position, morphology) and qualitative (bone mineral density (BMD)) therapeutic outcomes following a stabilization splint (S.S.) therapy in adult patients diagnosed with TMD (Arthralgia) with/without lateral mandibular asymmetry (MA) using cone beam computed tomography (CBCT).
    Methods: In this retrospective clinical study, 60 adult TMD patients who received S.S. therapy were enrolled and allocated into the TMD group (TMDG) and TMD with MA group (TMD + MAG). The diagnosis was made according to the Diagnostic Criteria for TMD (DC/TMD) AXIS I. MA was measured from the mid-sagittal plane to the Menton point. CBCT was used to scan the temporomandibular joints pre- (T0) and post- (T1)-treatment for three-dimensional analysis. Intra- and intergroup statistical comparisons were performed using the Wilcoxon signed ranks and the Kruskal‒Wallis test.
    Results: For quantitative comparisons, there was a statistically significant difference between T0 and T1 in the joint spaces of TMD + MAG (anterior, superior, posterior, and coronal lateral on the deviated side as well as in the superior, coronal medial joint space of the contralateral side). Morphologically, the deviated side had a narrower condylar width, reduced condylar height, and a steeper eminence angle. In contrast, the contralateral side tended to have a greater condylar length. For qualitative measurements, BMD also showed statistical significance between T0 and T1 in the majority of the condyle slopes (AS, SS, PS, and LS on the deviated side and in AS and MS on the contralateral side) of TMD + MAG. Additionally, only the AS and PS showed significance in TMDG.
    Conclusion: Multiple joint space widening (AJS and CMS) and narrowing (SJS, PJS, and CLS) could characterize the deviated side in TMD + MA. Factors like narrower condylar width, reduced condylar height, and steeper eminence angle on the deviated side can worsen TMD + MA. Proper alignment of the condyle-disc position is essential for optimal function and load distribution, potentially affecting bone mineral density (BMD). MA plays a prominent role in disturbing bone densities. S.S. therapy shows more evident outcomes in TMD + MAG (on the deviated side compared to the contralateral side) than the TMDG.
    MeSH term(s) Adult ; Humans ; Mandibular Condyle/diagnostic imaging ; Splints ; Retrospective Studies ; Temporomandibular Joint/diagnostic imaging ; Temporomandibular Joint Disorders/diagnostic imaging ; Temporomandibular Joint Disorders/therapy ; Cone-Beam Computed Tomography
    Language English
    Publishing date 2024-03-21
    Publishing country England
    Document type Journal Article
    ZDB-ID 2091511-1
    ISSN 1472-6831 ; 1472-6831
    ISSN (online) 1472-6831
    ISSN 1472-6831
    DOI 10.1186/s12903-024-04119-7
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Effect of Tensile Frequency on the Osteogenic Differentiation of Periodontal Ligament Stem Cells.

    Wang, Wenfang / Wang, Meijuan / Guo, Xiaomei / Zhao, Yunshan / Ahmed, Madiha Mohammed Saleh / Qi, Hong / Chen, Xi

    International journal of general medicine

    2022  Volume 15, Page(s) 5957–5971

    Abstract: Purpose: The role of periodontal ligament stem cells (PDLSCs) in mediating osteogenesis involved in orthodontic tooth movement (OTM) is well established. However, various relevant in vitro studies vary in the frequency of tension. The effect of tensile ... ...

    Abstract Purpose: The role of periodontal ligament stem cells (PDLSCs) in mediating osteogenesis involved in orthodontic tooth movement (OTM) is well established. However, various relevant in vitro studies vary in the frequency of tension. The effect of tensile frequency on the mechanotransduction of PDLSCs is not clear. The current study aimed to determine the effect of different tensile frequencies on the osteogenic differentiation of PDLSCs and to identify important mechano-sensitivity genes.
    Methods: Human PDLSCs were isolated, identified, and subjected to cyclic equibiaxial tensile strain of 12% at different frequencies of 0.1 Hz, 0.5 Hz, 0.7 Hz, or static cultures. Osteogenic differentiation of PDLSCs was assessed by using Western blotting. High-throughput sequencing was used to identify differential mRNA expression. Short time-series expression miner (STEM) was utilized to describe the frequency patterns of the mRNAs. The functions and enriched pathways were identified, and the hub genes were identified and validated.
    Results: We found that the osteoblastic differentiation capacity of PDLSCs increased with tensile frequency in the range of 0.1-0.7 Hz. Eight frequency-tendency gene expression profiles were identified to be statistically significant. Tensile frequency-specific expressed genes, such as SALL1 and EYA1, which decreased with the increase in tensile frequency, were found.
    Conclusion: The osteoblastic differentiation of PDLSCs under mechanical tensile force is frequency dependent. EYA1 and SALL1 were identified as potential important tensile frequency-sensitive genes, which may contribute to the cyclic tension-induced osteogenic differentiation of PDLSCs in a frequency-dependent manner.
    Language English
    Publishing date 2022-07-02
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2452220-X
    ISSN 1178-7074
    ISSN 1178-7074
    DOI 10.2147/IJGM.S368394
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: FAM3B

    Wang, Wenfang / Wang, Meijuan / Ahmed, Madiha Mohammed Saleh / Zhao, Yunshan / Wu, Hao / Musa, Mazen / Chen, Xi

    International journal of general medicine

    2022  Volume 15, Page(s) 763–776

    Abstract: Purpose: Oral lichen planus (OLP) is a potentially malignant condition with unclear etiology. This study aimed to identify potential biomarkers and mechanisms for OLP progression through bioinformatics analyses.: Methods: Gene Expression Omnibus (GEO) ...

    Abstract Purpose: Oral lichen planus (OLP) is a potentially malignant condition with unclear etiology. This study aimed to identify potential biomarkers and mechanisms for OLP progression through bioinformatics analyses.
    Methods: Gene Expression Omnibus (GEO) datasets were screened to identify differentially expressed genes (DEGs) between OLP patients and healthy individuals. The functions and enriched pathways of the DEGs were identified. Sequencing dataset GSE70665 was then used to analyze the role of DEGs in the development of OLP to oral squamous cell carcinoma (OSCC). Oncomine and The Cancer Genome Atlas (TCGA) databases were utilized to evaluate clinicopathological characters of OSCC. Univariate and multivariate Cox regression models were used to identify independent prognostic factors.
    Results: A total of 24 DEGs were identified between OLP and normal samples.
    Conclusion: Under-expression of
    Language English
    Publishing date 2022-01-20
    Publishing country New Zealand
    Document type Journal Article
    ZDB-ID 2452220-X
    ISSN 1178-7074
    ISSN 1178-7074
    DOI 10.2147/IJGM.S346617
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  6. Article ; Online: Three dimensional evaluation of the skeletal and temporomandibular joint changes following stabilization splint therapy in patients with temporomandibular joint disorders and mandibular deviation: a retrospective study.

    Ahmed, Madiha Mohammed Saleh / Shi, Danli / Al-Somairi, Majedh Abdo Ali / Alhashimi, Najah / Almashraqi, Abeer A / Musa, Mazen / Li, Ning / Chen, Xi / Alhammadi, Maged S

    BMC oral health

    2023  Volume 23, Issue 1, Page(s) 18

    Abstract: Background: Three-dimensional (3D) detailed evaluations of the mandibular mediolateral position, mandibular condylar position, and temporomandibular joint (TMJ) spaces following stabilization splints (SS) therapy in patients with temporomandibular joint ...

    Abstract Background: Three-dimensional (3D) detailed evaluations of the mandibular mediolateral position, mandibular condylar position, and temporomandibular joint (TMJ) spaces following stabilization splints (SS) therapy in patients with temporomandibular joint disorders (TMD) and mandibular deviation (MD) have not been reported in the available literature. Accordingly, this study aimed to three-dimensionally analyze the skeletal and bony temporomandibular joint changes following stabilization splint therapy in adult patients with temporomandibular joint disorders and mandibular deviation.
    Methods: This study is a retrospective clinical study that enrolled 26 adult patients with TMD and MD with a mean age of 24.86 years. The Diagnostic Criteria for Temporomandibular Disorders (DC/TMD) was used to diagnose TMD. SS was adjusted weekly until occlusal contact stabilization occurred, and then adjusted monthly, patients were instructed to wear it at night for at least 10 h. The SS was removed after the elimination of TMD symptoms (TMJ/muscle pain on palpation, muscle spasm, and clicking) and having both condyles completely seated in a musculoskeletally stable position. Pre- and post-therapeutic Cone Beam Computed Tomography (CBCT) was analyzed. Mandibular mediolateral position, TMJ spaces, and mandibular condyle position were analyzed three-dimensionally using Mimics 21.0 software. Paired t-test or Wilcoxon rank-sum test was performed, and the significance level was considered at P < 0.05.
    Results: The treatment period with SS therapy was 10.07 ± 3.1 months. The deviated chin was improved in 69.23% of the sample; the range of improvement was > 0 mm ≤ 3.9 mm. The mandibular rotation was significantly decreased from 3.58 ± 2.02° to 3.17 ± 1.60. The deviated side's superior and posterior joint TMJ spaces were significantly increased from 2.49 ± 0.88 mm and 1.25 ± 0.79 mm to 2.98 ± 1.02 mm and 1.86 ± 0.72 mm, respectively. The value of the difference from the bilateral condyle head position to the X and Z axes significantly decreased from 2.50 ± 1.56 mm and 2.30 ± 1.57 mm to 1.64 ± 1.58 mm and 1.82 ± 1.11 mm, respectively.
    Conclusion: The main positional effect of the stabilization splint treatment in TMD patients with MD includes considerable correction of mandibular deviation, improving facial asymmetry, and moving the condyle into a stable condylar position; these were done by promoting the mandible to rotate around the Z (roll) and Y (yaw) axes and by forward, downward, and outward condylar movement on the deviated side, respectively.
    MeSH term(s) Adult ; Humans ; Young Adult ; Malocclusion/diagnostic imaging ; Malocclusion/therapy ; Mandibular Condyle/diagnostic imaging ; Retrospective Studies ; Splints ; Temporomandibular Joint/diagnostic imaging ; Temporomandibular Joint Disorders/diagnostic imaging ; Temporomandibular Joint Disorders/therapy ; Occlusal Splints
    Language English
    Publishing date 2023-01-13
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2091511-1
    ISSN 1472-6831 ; 1472-6831
    ISSN (online) 1472-6831
    ISSN 1472-6831
    DOI 10.1186/s12903-023-02720-w
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Three-dimensional evaluation of upper pharyngeal airway, hyoid bone, and craniocervical changes following stabilization splint therapy in adult patients with temporomandibular joint disorders and mandibular deviation: A retrospective study.

    Ahmed, Madiha Mohammed Saleh / Zhao, Yunshan / Al-Hadad, Saba Ahmed / Li, Chunshen / Wang, Lu / Hu, Wangqing / Ahmed, Moutaz Mohammed Saleh / Musa, Mazen / Chen, Xi / Alhammadi, Maged S

    Journal of stomatology, oral and maxillofacial surgery

    2023  Volume 124, Issue 6S2, Page(s) 101646

    Abstract: Objective: This study aimed primarily to analyze the three-dimensional (3D) changes in the pharyngeal airway (PA), and secondarily, the hyoid bone (HB) and the craniocervical (CC) following stabilization splint (SS) therapy in adult patients with ... ...

    Abstract Objective: This study aimed primarily to analyze the three-dimensional (3D) changes in the pharyngeal airway (PA), and secondarily, the hyoid bone (HB) and the craniocervical (CC) following stabilization splint (SS) therapy in adult patients with temporomandibular joint disorders (TMD) and mandibular deviation (MD).
    Methods: Thirty-five adult patients with TMD and MD, who were treated using SS with a mean age of 25.14 ± 6.11 years, were enrolled in this retrospective clinical study. Pre- and post-therapeutic cone-beam computed tomography (CBCT) scans were analyzed. PA dimension,nasopharyngeal, oropharyngeal, hypopharyngeal, sub-hypopharyngeal, and total pharyngeal airway spaces were measured in surface area, volume, minimum constricted area (MCA) and width, HB position, and CC posture were analyzed three-dimensionally using InVivo 6.0.3 and Dolphin 11.95 software. Wilcoxon rank-sum or Paired t-test was conducted, and P < 0.05 was considered significant.
    Results: SS therapy was administered for a period of 9.49 ± 4.02 months. The oropharyngeal airway space showed a significant decrease in sagittal width. The hypopharyngeal surface area, volume, MCA, and sagittal width decreased significantly. In terms of HB, hyoid-mandibular plane (H-MP), retrognathia-third vertebra's most inferior-anterior (RGN-C3ia), and retrognathia-Sella (RGN-S) distances significantly decreased. The Nasion-Sella line and the line that passes through C2ip to the odontoid process posterior tangent (NSL-OPT) angle in CC posture also decreased significantly.
    Conclusion: SS therapy in TMD patients with MD mainly results in narrowing of the hypopharyngeal region, no change in HB position and improvement in head posture. These results undoubtedly assist in diagnosis and treatment of clinical conditions.
    MeSH term(s) Adult ; Humans ; Young Adult ; Hyoid Bone/diagnostic imaging ; Retrospective Studies ; Malocclusion, Angle Class III ; Retrognathia ; Splints ; Cephalometry/methods ; Malocclusion ; Temporomandibular Joint Disorders/diagnostic imaging ; Temporomandibular Joint Disorders/therapy
    Language English
    Publishing date 2023-09-24
    Publishing country France
    Document type Journal Article
    ZDB-ID 2916276-2
    ISSN 2468-7855 ; 2468-8509
    ISSN (online) 2468-7855
    ISSN 2468-8509
    DOI 10.1016/j.jormas.2023.101646
    Database MEDical Literature Analysis and Retrieval System OnLINE

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