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  1. Article ; Online: Sulfation modification of dopamine in brain regulates aggregative behavior of animals.

    Chen, Bing / Tong, Xiwen / Zhang, Xia / Gui, Wanying / Ai, Guoming / Huang, Lihua / Ding, Ding / Zhang, Jiangxu / Kang, Le

    National science review

    2021  Volume 9, Issue 4, Page(s) nwab163

    Abstract: Behavioral plasticity and the underlying neuronal plasticity represent a fundamental capacity of animals to cope with environmental stimuli. Behavioral plasticity is controlled by complex molecular networks that act under different layers of regulation. ... ...

    Abstract Behavioral plasticity and the underlying neuronal plasticity represent a fundamental capacity of animals to cope with environmental stimuli. Behavioral plasticity is controlled by complex molecular networks that act under different layers of regulation. While various molecules have been found to be involved in the regulation of plastic behaviors across species, less is known about how organisms orchestrate the activity of these molecules as part of a coherent behavioral response to varying environments. Here we discover a mechanism for the regulation of animal behavioral plasticity involving molecular sulfation in the brain, a modification of substrate molecules by sulfotransferase (ST)-catalyzed addition of a sulfonate group (SO
    Language English
    Publishing date 2021-09-02
    Publishing country China
    Document type Journal Article
    ZDB-ID 2745465-4
    ISSN 2053-714X ; 2053-714X
    ISSN (online) 2053-714X
    ISSN 2053-714X
    DOI 10.1093/nsr/nwab163
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Tandem expression in E. coli of type III PKS and P450 genes from marine Streptomyces olivaceus FXJ 7.023 gives production of phenol and indole

    Yue, Changwu / Liu, Ning / Liu, Minghao / Lü, Yuhong / Shao, Meiyun / Wang, Miao / Ai, Guoming / Huang, Ying

    World journal of microbiology & biotechnology. 2015 Apr., v. 31, no. 4

    2015  

    Abstract: The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III ... ...

    Abstract The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III PKS (SoRppA) gene and its adjacent gene for cytochrome P450 158A2 (SoCYP158A2) of this gene cluster were cloned by PCR screening through a fosmid genomic library of S. olivaceus FXJ 7.023. Tandem expression of SoRppA and SoCYP158A2 in Escherichia coli strain BL21 (DE3) plysS resulted in obvious biosynthesis of phenol and indole, while heterologous expression of SoRppA or SoCYP158A2 alone did not. The engineered strain sorppAcyp158a2BL21 showed tolerance to phenol concentration up to 0.75 g/L. Continuous biosynthesis of phenol and indole by the immobilized engineered strain on macroporousresin was achieved, and the productivities of phenol and indole in extractant-free culture in 102 h were 0.08 and 1.525 g/L/h, respectively, with the highest production reached 0.67 and 14.48 g/L, respectively. These results suggest that the engineered strain and immobilized continuous fermentation process may provide potential for “green” production of phenol and indole.
    Keywords Escherichia coli ; Streptomyces coelicolor ; Streptomyces olivaceus ; biosynthesis ; biotechnology ; continuous fermentation ; cytochrome P-450 ; genes ; genomic libraries ; microbiology ; nucleotide sequences ; phenol ; polyketide synthases ; polymerase chain reaction ; screening
    Language English
    Dates of publication 2015-04
    Size p. 541-548.
    Publishing place Springer-Verlag
    Document type Article
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-015-1825-2
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Tandem expression in E. coli of type III PKS and P450 genes from marine Streptomyces olivaceus FXJ 7.023 gives production of phenol and indole.

    Yue, Changwu / Liu, Ning / Liu, Minghao / Lü, Yuhong / Shao, Meiyun / Wang, Miao / Ai, Guoming / Huang, Ying

    World journal of microbiology & biotechnology

    2015  Volume 31, Issue 4, Page(s) 541–548

    Abstract: The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III ... ...

    Abstract The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III PKS (SoRppA) gene and its adjacent gene for cytochrome P450 158A2 (SoCYP158A2) of this gene cluster were cloned by PCR screening through a fosmid genomic library of S. olivaceus FXJ 7.023. Tandem expression of SoRppA and SoCYP158A2 in Escherichia coli strain BL21 (DE3) plysS resulted in obvious biosynthesis of phenol and indole, while heterologous expression of SoRppA or SoCYP158A2 alone did not. The engineered strain sorppAcyp158a2BL21 showed tolerance to phenol concentration up to 0.75 g/L. Continuous biosynthesis of phenol and indole by the immobilized engineered strain on macroporousresin was achieved, and the productivities of phenol and indole in extractant-free culture in 102 h were 0.08 and 1.525 g/L/h, respectively, with the highest production reached 0.67 and 14.48 g/L, respectively. These results suggest that the engineered strain and immobilized continuous fermentation process may provide potential for "green" production of phenol and indole.
    MeSH term(s) Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Cytochrome P-450 Enzyme System/genetics ; Cytochrome P-450 Enzyme System/metabolism ; Escherichia coli/genetics ; Escherichia coli/metabolism ; Gene Expression Regulation, Bacterial ; Indoles/metabolism ; Metabolic Engineering ; Phenol/metabolism ; Polyketide Synthases/genetics ; Polyketide Synthases/metabolism ; Seawater/microbiology ; Streptomyces/enzymology ; Streptomyces/genetics
    Chemical Substances Bacterial Proteins ; Indoles ; Phenol (339NCG44TV) ; Polyketide Synthases (79956-01-7) ; indole (8724FJW4M5) ; Cytochrome P-450 Enzyme System (9035-51-2)
    Language English
    Publishing date 2015-04
    Publishing country Germany
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1499109-3
    ISSN 1573-0972 ; 0959-3993
    ISSN (online) 1573-0972
    ISSN 0959-3993
    DOI 10.1007/s11274-015-1825-2
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  4. Article: Tandem expression in E. coli of type III PKS and P450 genes from marine Streptomyces olivaceus FXJ 7.023 gives production of phenol and indole

    Yue, Changwu / Liu, Ning / Liu, Minghao / Lü, Yuhong / Shao, Meiyun / Wang, Miao / Ai, Guoming / Huang, Ying

    World journal of microbiology & biotechnology

    Volume v. 31,, Issue no. 4

    Abstract: The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III ... ...

    Abstract The draft genome sequence of marine Streptomyces olivaceus strain FXJ 7.023 contains a cryptic Type III polyketide synthase (type III PKS) gene cluster, which is similar to the Streptomyces coelicolor THN biosynthesis gene cluster. A putative type III PKS (SoRppA) gene and its adjacent gene for cytochrome P450 158A2 (SoCYP158A2) of this gene cluster were cloned by PCR screening through a fosmid genomic library of S. olivaceus FXJ 7.023. Tandem expression of SoRppA and SoCYP158A2 in Escherichia coli strain BL21 (DE3) plysS resulted in obvious biosynthesis of phenol and indole, while heterologous expression of SoRppA or SoCYP158A2 alone did not. The engineered strain sorppAcyp158a2BL21 showed tolerance to phenol concentration up to 0.75 g/L. Continuous biosynthesis of phenol and indole by the immobilized engineered strain on macroporousresin was achieved, and the productivities of phenol and indole in extractant-free culture in 102 h were 0.08 and 1.525 g/L/h, respectively, with the highest production reached 0.67 and 14.48 g/L, respectively. These results suggest that the engineered strain and immobilized continuous fermentation process may provide potential for “green” production of phenol and indole.
    Keywords genomic libraries ; cytochrome P-450 ; phenol ; genes ; polyketide synthases ; Streptomyces coelicolor ; Streptomyces olivaceus ; continuous fermentation ; biotechnology ; screening ; nucleotide sequences ; Escherichia coli ; biosynthesis ; polymerase chain reaction ; microbiology
    Language English
    Document type Article
    ISSN 0959-3993
    Database AGRIS - International Information System for the Agricultural Sciences and Technology

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