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  1. Article ; Online: Two adjacent NLR genes conferring quantitative resistance to clubroot disease in Arabidopsis are regulated by a stably inherited epiallelic variation.

    Gravot, Antoine / Liégard, Benjamin / Quadrana, Leandro / Veillet, Florian / Aigu, Yoann / Bargain, Tristan / Bénéjam, Juliette / Lariagon, Christine / Lemoine, Jocelyne / Colot, Vincent / Manzanares-Dauleux, Maria J / Jubault, Mélanie

    Plant communications

    2024  Volume 5, Issue 5, Page(s) 100824

    Abstract: Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassicaceae. Using a combination of quantitative trait locus (QTL) fine mapping, CRISPR-Cas9 validation, and extensive analyses of DNA sequence and ... ...

    Abstract Clubroot caused by the protist Plasmodiophora brassicae is a major disease affecting cultivated Brassicaceae. Using a combination of quantitative trait locus (QTL) fine mapping, CRISPR-Cas9 validation, and extensive analyses of DNA sequence and methylation patterns, we revealed that the two adjacent neighboring NLR (nucleotide-binding and leucine-rich repeat) genes AT5G47260 and AT5G47280 cooperate in controlling broad-spectrum quantitative partial resistance to the root pathogen P. brassicae in Arabidopsis and that they are epigenetically regulated. The variation in DNA methylation is not associated with any nucleotide variation or any transposable element presence/absence variants and is stably inherited. Variations in DNA methylation at the Pb-At5.2 QTL are widespread across Arabidopsis accessions and correlate negatively with variations in expression of the two genes. Our study demonstrates that natural, stable, and transgenerationally inherited epigenetic variations can play an important role in shaping resistance to plant pathogens by modulating the expression of immune receptors.
    MeSH term(s) Arabidopsis/genetics ; Arabidopsis/immunology ; Plant Diseases/genetics ; Plant Diseases/immunology ; Plant Diseases/parasitology ; Disease Resistance/genetics ; NLR Proteins/genetics ; NLR Proteins/metabolism ; DNA Methylation ; Plasmodiophorida/physiology ; Quantitative Trait Loci/genetics ; Arabidopsis Proteins/genetics ; Epigenesis, Genetic ; Gene Expression Regulation, Plant ; Genes, Plant ; Alleles
    Language English
    Publishing date 2024-01-23
    Publishing country China
    Document type Journal Article
    ISSN 2590-3462
    ISSN (online) 2590-3462
    DOI 10.1016/j.xplc.2024.100824
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Multi-Omic Investigation of Low-Nitrogen Conditional Resistance to Clubroot Reveals

    Aigu, Yoann / Daval, Stéphanie / Gazengel, Kévin / Marnet, Nathalie / Lariagon, Christine / Laperche, Anne / Legeai, Fabrice / Manzanares-Dauleux, Maria J / Gravot, Antoine

    Frontiers in plant science

    2022  Volume 13, Page(s) 790563

    Abstract: Nitrogen fertilization has been reported to influence the development of clubroot, a root disease ... ...

    Abstract Nitrogen fertilization has been reported to influence the development of clubroot, a root disease of
    Language English
    Publishing date 2022-02-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2022.790563
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article: Nitrogen Supply and Host-Plant Genotype Modulate the Transcriptomic Profile of

    Gazengel, Kévin / Aigu, Yoann / Lariagon, Christine / Humeau, Mathilde / Gravot, Antoine / Manzanares-Dauleux, Maria J / Daval, Stéphanie

    Frontiers in microbiology

    2021  Volume 12, Page(s) 701067

    Abstract: Nitrogen fertilization can affect the susceptibility ... ...

    Abstract Nitrogen fertilization can affect the susceptibility of
    Language English
    Publishing date 2021-07-08
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2021.701067
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  4. Article: Computational analysis of the Plasmodiophora brassicae genome: mitochondrial sequence description and metabolic pathway database design

    Daval, Stéphanie / Aigu, Yoann / Belcour, Arnaud / Cottret, Ludovic / Gazengel, Kévin / Gouzy, Jérôme / Lebreton, Lionel / Legrand, Ludovic / Manzanares-Dauleux, Maria J / Mougel, Christophe

    Genomics. 2019 Dec., v. 111, no. 6

    2019  

    Abstract: Plasmodiophora brassicae is an obligate biotrophic pathogenic protist responsible for clubroot, a root gall disease of Brassicaceae species. In addition to the reference genome of the P. brassicae European e3 isolate and the draft genomes of Canadian or ... ...

    Abstract Plasmodiophora brassicae is an obligate biotrophic pathogenic protist responsible for clubroot, a root gall disease of Brassicaceae species. In addition to the reference genome of the P. brassicae European e3 isolate and the draft genomes of Canadian or Chinese isolates, we present the genome of eH, a second European isolate. Refinement of the annotation of the eH genome led to the identification of the mitochondrial genome sequence, which was found to be bigger than that of Spongospora subterranea, another plant parasitic Plasmodiophorid phylogenetically related to P. brassicae. New pathways were also predicted, such as those for the synthesis of spermidine, a polyamine up-regulated in clubbed regions of roots. A P. brassicae pathway genome database was created to facilitate the functional study of metabolic pathways in transcriptomics approaches. These available tools can help in our understanding of the regulation of P. brassicae metabolism during infection and in response to diverse constraints.
    Keywords biochemical pathways ; Brassicaceae ; clubroot ; databases ; metabolism ; mitochondria ; mitochondrial genome ; nucleotide sequences ; phylogeny ; Plasmodiophora brassicae ; protists ; root galls ; roots ; spermidine ; Spongospora subterranea ; transcriptomics
    Language English
    Dates of publication 2019-12
    Size p. 1629-1640.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 356334-0
    ISSN 1089-8646 ; 0888-7543
    ISSN (online) 1089-8646
    ISSN 0888-7543
    DOI 10.1016/j.ygeno.2018.11.013
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2367: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  5. Article ; Online: Computational analysis of the Plasmodiophora brassicae genome: mitochondrial sequence description and metabolic pathway database design.

    Daval, Stéphanie / Belcour, Arnaud / Gazengel, Kévin / Legrand, Ludovic / Gouzy, Jérôme / Cottret, Ludovic / Lebreton, Lionel / Aigu, Yoann / Mougel, Christophe / Manzanares-Dauleux, Maria J

    Genomics

    2018  Volume 111, Issue 6, Page(s) 1629–1640

    Abstract: Plasmodiophora brassicae is an obligate biotrophic pathogenic protist responsible for clubroot, a root gall disease of Brassicaceae species. In addition to the reference genome of the P. brassicae European e3 isolate and the draft genomes of Canadian or ... ...

    Abstract Plasmodiophora brassicae is an obligate biotrophic pathogenic protist responsible for clubroot, a root gall disease of Brassicaceae species. In addition to the reference genome of the P. brassicae European e3 isolate and the draft genomes of Canadian or Chinese isolates, we present the genome of eH, a second European isolate. Refinement of the annotation of the eH genome led to the identification of the mitochondrial genome sequence, which was found to be bigger than that of Spongospora subterranea, another plant parasitic Plasmodiophorid phylogenetically related to P. brassicae. New pathways were also predicted, such as those for the synthesis of spermidine, a polyamine up-regulated in clubbed regions of roots. A P. brassicae pathway genome database was created to facilitate the functional study of metabolic pathways in transcriptomics approaches. These available tools can help in our understanding of the regulation of P. brassicae metabolism during infection and in response to diverse constraints.
    MeSH term(s) DNA, Mitochondrial/genetics ; DNA, Mitochondrial/metabolism ; DNA, Protozoan/genetics ; DNA, Protozoan/metabolism ; Databases, Genetic ; Genome, Mitochondrial ; Genome, Protozoan ; Metabolic Networks and Pathways/physiology ; Phylogeny ; Plasmodiophorida/genetics ; Plasmodiophorida/metabolism
    Chemical Substances DNA, Mitochondrial ; DNA, Protozoan
    Language English
    Publishing date 2018-11-15
    Publishing country United States
    Document type Journal Article
    ZDB-ID 356334-0
    ISSN 1089-8646 ; 0888-7543
    ISSN (online) 1089-8646
    ISSN 0888-7543
    DOI 10.1016/j.ygeno.2018.11.013
    Database MEDical Literature Analysis and Retrieval System OnLINE

    Full text online

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 2367: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

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  6. Article: Corrigendum: Optimization of Agroinfiltration in

    Guy, Endrick / Boulain, Hélène / Aigu, Yoann / Le Pennec, Charlotte / Chawki, Khaoula / Morlière, Stéphanie / Schädel, Kristina / Kunert, Grit / Simon, Jean-Christophe / Sugio, Akiko

    Frontiers in plant science

    2017  Volume 7, Page(s) 2046

    Abstract: This corrects the article on p. 1171 in vol. 7, PMID: 27555856.]. ...

    Abstract [This corrects the article on p. 1171 in vol. 7, PMID: 27555856.].
    Language English
    Publishing date 2017-01-10
    Publishing country Switzerland
    Document type Published Erratum
    ZDB-ID 2711035-7
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2016.02046
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article: Optimization of Agroinfiltration in Pisum sativum Provides a New Tool for Studying the Salivary Protein Functions in the Pea Aphid Complex.

    Guy, Endrick / Boulain, Hélène / Aigu, Yoann / Le Pennec, Charlotte / Chawki, Khaoula / Morlière, Stéphanie / Schädel, Kristina / Kunert, Grit / Simon, Jean-Christophe / Sugio, Akiko

    Frontiers in plant science

    2016  Volume 7, Page(s) 1171

    Abstract: Aphids are piercing-sucking insect pests and feed on phloem sap. During feeding, aphids inject a battery of salivary proteins into host plant. Some of these proteins function like effectors of microbial pathogens and influence the outcome of plant-aphid ... ...

    Abstract Aphids are piercing-sucking insect pests and feed on phloem sap. During feeding, aphids inject a battery of salivary proteins into host plant. Some of these proteins function like effectors of microbial pathogens and influence the outcome of plant-aphid interactions. The pea aphid (Acyrthosiphon pisum) is the model aphid and encompasses multiple biotypes each specialized to one or a few legume species, providing an opportunity to investigate the underlying mechanisms of the compatibility between plants and aphid biotypes. We aim to identify the aphid factors that determine the compatibility with host plants, hence involved in the host plant specialization process, and hypothesize that salivary proteins are one of those factors. Agrobacterium-mediated transient gene expression is a powerful tool to perform functional analyses of effector (salivary) proteins in plants. However, the tool was not established for the legume species that A. pisum feeds on. Thus, we decided to optimize the method for legume plants to facilitate the functional analyses of A. pisum salivary proteins. We screened a range of cultivars of pea (Pisum sativum) and alfalfa (Medicago sativa). None of the M. sativa cultivars was suitable for agroinfiltration under the tested conditions; however, we established a protocol for efficient transient gene expression in two cultivars of P. sativum, ZP1109 and ZP1130, using A. tumefaciens AGL-1 strain and the pEAQ-HT-DEST1 vector. We confirmed that the genes are expressed from 3 to 10 days post-infiltration and that aphid lines of the pea adapted biotype fed and reproduced on these two cultivars while lines of alfalfa and clover biotypes did not. Thus, the pea biotype recognizes these two cultivars as typical pea plants. By using a combination of ZP1109 and an A. pisum line, we defined an agroinfiltration procedure to examine the effect of in planta expression of selected salivary proteins on A. pisum fitness and demonstrated that transient expression of one candidate salivary gene increased the fecundity of the aphids. This result confirms that the agroinfiltration can be used to perform functional analyses of salivary proteins in P. sativum and consequently to study the molecular mechanisms underlying host specialization in the pea aphid complex.
    Language English
    Publishing date 2016-08-09
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2711035-7
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2016.01171
    Database MEDical Literature Analysis and Retrieval System OnLINE

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