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Artikel ; Online: Improved Microcirculation with Pulsatile Veno-Arterial ECMO: An Ovine Model of Cardiogenic Shock.

Heinsar, Silver / Sato, Kei / Obonyo, Nchafatso / Farah, Samia M / Bouquet, Mahe / Passmore, Margaret R / Liu, Keibun / Ijunin, Shinichi / Ainola, Carmen / Li Bassi, Gianluigi / Suen, Jacky Y / Fraser, John F

American journal of respiratory and critical care medicine

2024

Sprache	Englisch
Erscheinungsdatum	2024-03-18
Erscheinungsland	United States
Dokumenttyp	Journal Article
ZDB-ID	1180953-x
ISSN	1535-4970 ; 0003-0805 ; 1073-449X
ISSN (online)	1535-4970
ISSN	0003-0805 ; 1073-449X
DOI	10.1164/rccm.202402-0419LE
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation

Zhang, Meili / Chan, Chris H.H. / Pauls, Jo P. / Semenzin, Clayton / Ainola, Carmen / Peng, Hui / Fu, Changkui / Whittaker, Andrew K. / Heinsar, Silver / Fraser, John F.

Journal of materials chemistry B. 2022 July 6, v. 10, no. 26

2022

Abstract: Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia ... ...

Abstract	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit. From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit. Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application. Unfractionated heparin is the most commonly used anticoagulant for ECMO. In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated. The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution. Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel. Our results showed that increased crosslinker content and decreased water content led to slower heparin release. The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA. The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood. The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes. The coatings showed sustained heparin release with a cumulative release of 70–80% after 7 days. Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time. These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
Schlagwörter	adhesion ; anticoagulants ; biocompatible materials ; crosslinking ; hemostasis ; heparin ; hydrogels ; hydrophilicity ; hyperoxia ; water content
Sprache	Englisch
Erscheinungsverlauf	2022-0706
Umfang	p. 4974-4983.
Erscheinungsort	The Royal Society of Chemistry
Dokumenttyp	Artikel
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d2tb00379a
Datenquelle	NAL Katalog (AGRICOLA)

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Artikel ; Online: An appraisal of lung computer tomography in very early anti-inflammatory treatment of two different ovine ARDS phenotypes.

Wildi, Karin / Colombo, Sebastiano Maria / McGuire, Daniel / Ainola, Carmen / Heinsar, Silver / Sato, Noriko / Sato, Kei / Liu, Keibun / Bouquet, Mahé / Wilson, Emily / Passmore, Margaret / Hyslop, Kieran / Livingstone, Samantha / Di Feliciantonio, Marianna / Strugnell, Wendy / Palmieri, Chiara / Suen, Jacky / Li Bassi, Gianluigi / Fraser, John

Scientific reports

2024 Band 14, Heft 1, Seite(n) 2162

Abstract: Mortality and morbidity of Acute Respiratory Distress Syndrome (ARDS) are largely unaltered. A possible new approach to treatment of ARDS is offered by the discovery of inflammatory subphenotypes. In an ovine model of ARDS phenotypes, matching key ... ...

Abstract	Mortality and morbidity of Acute Respiratory Distress Syndrome (ARDS) are largely unaltered. A possible new approach to treatment of ARDS is offered by the discovery of inflammatory subphenotypes. In an ovine model of ARDS phenotypes, matching key features of the human subphenotypes, we provide an imaging characterization using computer tomography (CT). Nine animals were randomized into (a) OA (oleic acid, hypoinflammatory; n = 5) and (b) OA-LPS (oleic acid and lipopolysaccharides, hyperinflammatory; n = 4). 48 h after ARDS induction and anti-inflammatory treatment, CT scans were performed at high (H) and then low (L) airway pressure. After CT, the animals were euthanized and lung tissue was collected. OA-LPS showed a higher air fraction and OA a higher tissue fraction, resulting in more normally aerated lungs in OA-LPS in contrast to more non-aerated lung in OA. The change in lung and air volume between H and L was more accentuated in OA-LPS, indicating a higher recruitment potential. Strain was higher in OA, indicating a higher level of lung damage, while the amount of lung edema and histological lung injury were largely comparable. Anti-inflammatory treatment might be beneficial in terms of overall ventilated lung portion and recruitment potential, especially in the OA-LPS group.
Mesh-Begriff(e)	Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Lipopolysaccharides ; Lung/pathology ; Oleic Acid/pharmacology ; Phenotype ; Respiratory Distress Syndrome/pathology ; Sheep ; Sheep, Domestic ; Tomography
Chemische Substanzen	Anti-Inflammatory Agents ; Lipopolysaccharides ; Oleic Acid (2UMI9U37CP)
Sprache	Englisch
Erscheinungsdatum	2024-01-25
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52698-w
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Comparative analysis of novel esophageal pressure monitoring catheters versus commercially available alternatives in a biomechanical model of the thoracic cavity.

Abbate, Gabriella / Colombo, Sebastiano Maria / Semenzin, Clayton / Sato, Noriko / Liu, Keibun / Ainola, Carmen / Milani, Angelo / Fior, Gabriele / Obonyo, Nchafatso / White, Nicole / Chiumello, Davide / Pauls, Jo / Suen, Jacky Y / Fraser, John F / Li Bassi, Gianluigi

Scientific reports

2024 Band 14, Heft 1, Seite(n) 9771

Abstract: Transpulmonary pressure can be estimated using esophageal balloon (EB) catheters, which come in a variety of manufacturing configurations. We assessed the performance of novel polyurethane EB designs, Aspisafe NG and NG+, against existing alternatives. ... ...

Abstract	Transpulmonary pressure can be estimated using esophageal balloon (EB) catheters, which come in a variety of manufacturing configurations. We assessed the performance of novel polyurethane EB designs, Aspisafe NG and NG+, against existing alternatives. We created a biomechanical model of the chest cavity using a plastic chamber and an ex-vivo porcine esophagus. The chamber was pressurized (- 20 and + 20 cmH
Mesh-Begriff(e)	Animals ; Esophagus/physiology ; Swine ; Catheters ; Pressure ; Thoracic Cavity ; Biomechanical Phenomena ; Polyurethanes/chemistry ; Monitoring, Physiologic/methods ; Monitoring, Physiologic/instrumentation
Chemische Substanzen	Polyurethanes
Sprache	Englisch
Erscheinungsdatum	2024-04-29
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't ; Comparative Study
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-59790-1
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: A novel speckle-tracking echocardiography parameter assessing left ventricular afterload.

Sato, Kei / Wildi, Karin / Chan, Jonathan / Palmieri, Chiara / Obonyo, Nchafatso G / Heinsar, Silver / Liu, Keibun / Livingstone, Samantha / Sato, Noriko / Ainola, Carmen / Abbate, Gabriella / Bouquet, Mahé / Wilson, Emily / Passmore, Margaret / Hyslop, Kieran / Platts, David G / Suen, Jacky / Bassi, Gianluigi Li / Fraser, John F

European journal of clinical investigation

2023 Band 54, Heft 2, Seite(n) e14106

Abstract: Background: Left ventricular stroke work index (LVSWI) and afterload-related cardiac performance (ACP) consider left ventricular (LV) afterload and could be better prognosticators in septic cardiomyopathy. However, their invasive nature prevents their ... ...

Abstract	Background: Left ventricular stroke work index (LVSWI) and afterload-related cardiac performance (ACP) consider left ventricular (LV) afterload and could be better prognosticators in septic cardiomyopathy. However, their invasive nature prevents their routine clinical applications. This study aimed to investigate (1) whether a proposed speckle-tracking echocardiography parameter, Pressure-Strain Product (PSP), can non-invasively predict catheter-based LVSWI, ACP and serum lactate in an ovine model of septic cardiomyopathy; and (2) whether PSP can distinguish the sub-phenotypes of acute respiratory distress syndrome (ARDS) with or without sepsis-like conditions. Methods: Sixteen sheep with ARDS were randomly assigned to either (1) sepsis-like (n = 8) or (2) non-sepsis-like (n = 8) group. Each ARDS and sepsis-like condition was induced by intravenous infusion of oleic acid and lipopolysaccharide, respectively. Pulmonary artery catheter-based LVSWI (the product of stroke work index, mean arterial pressure and .0136), ACP (the percentage of cardiac output measured to cardiac output predicted as normal) and serum lactate were measured simultaneously with transthoracic echocardiography. Two PSP indices were calculated by multiplying the mean arterial blood pressure and either global circumferential strain (PSPcirc) or radial strain (PSPrad). Results: PSPcirc showed a significant correlation with LVSWI (r Conclusions: A novel PSP has the potential to non-invasively predict catheter-based LVSWI and ACP, and was associated with serum lactate in septic cardiomyopathy.
Mesh-Begriff(e)	Animals ; Sheep ; Echocardiography ; Stroke Volume ; Ventricular Function, Left ; Sepsis ; Cardiomyopathies ; Respiratory Distress Syndrome ; Lactates ; Stroke ; Ventricular Dysfunction, Left/diagnostic imaging
Chemische Substanzen	Lactates
Sprache	Englisch
Erscheinungsdatum	2023-10-11
Erscheinungsland	England
Dokumenttyp	Journal Article
ZDB-ID	186196-7
ISSN	1365-2362 ; 0014-2972 ; 0960-135X
ISSN (online)	1365-2362
ISSN	0014-2972 ; 0960-135X
DOI	10.1111/eci.14106
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Investigation of heparin-loaded poly(ethylene glycol)-based hydrogels as anti-thrombogenic surface coatings for extracorporeal membrane oxygenation.

Zhang, Meili / Chan, Chris H H / Pauls, Jo P / Semenzin, Clayton / Ainola, Carmen / Peng, Hui / Fu, Changkui / Whittaker, Andrew K / Heinsar, Silver / Fraser, John F

Journal of materials chemistry. B

2022 Band 10, Heft 26, Seite(n) 4974–4983

Abstract	Extracorporeal membrane oxygenation (ECMO), a critical life-sustaining tool, faces significant challenges for the maintenance of normal haemostasis due to the large volume of circulating blood continuously in contact with artificial surfaces, hyperoxia and excessive shear stresses of the extracorporeal circuit. From a biomaterials perspective, it has been hypothesised that drug eluting coatings composed of haemocompatible hydrogels loaded with an anticoagulant drug could potentially enhance the haemocompatibility of the circuit. Poly(ethylene glycol) (PEG) has been well established as a biocompatible and anti-fouling material with wide biomedical application. Unfractionated heparin is the most commonly used anticoagulant for ECMO. In the present study, the feasibility of using heparin-loaded PEG-based hydrogels as anti-thrombogenic surface coatings for ECMO was investigated. The hydrogels were synthesised by photopolymerisation using poly(ethylene glycol) diacrylate (PEGDA) as the crosslinking monomer and poly(ethylene glycol) methacrylate (PEGMA) as the hydrophilic monomer, with heparin loaded into the pre-gel solution. Factors which could affect the release of heparin were investigated, including the ratio of PEGDA/PEGMA, water content, loading level of heparin and the flow of fluid past the hydrogel. Our results showed that increased crosslinker content and decreased water content led to slower heparin release. The hydrogels with water contents of 60 wt% and 70 wt% could achieve a sustained heparin release by adjusting the ratio of PEGDA/PEGMA. The anticoagulation efficacy of the released heparin was evaluated by measuring the activated clotting time of whole blood. The hydrogels with desirable heparin release profiles were prepared onto poly(4-methyl-1-pentene) (PMP) films with the same chemical composition as the PMP ECMO membranes. The coatings showed sustained heparin release with a cumulative release of 70-80% after 7 days. Haemocompatibility tests demonstrated that PEG hydrogel coatings significantly reduced platelet adhesion and prolonged plasma recalcification time. These results suggest that heparin-loaded PEG hydrogels are potential anti-thrombogenic coatings for ECMO.
Mesh-Begriff(e)	Biocompatible Materials/chemistry ; Extracorporeal Membrane Oxygenation ; Heparin/pharmacology ; Hydrogels/chemistry ; Hydrogels/pharmacology ; Polyethylene Glycols/chemistry ; Water
Chemische Substanzen	Biocompatible Materials ; Hydrogels ; Water (059QF0KO0R) ; Polyethylene Glycols (3WJQ0SDW1A) ; Heparin (9005-49-6)
Sprache	Englisch
Erscheinungsdatum	2022-07-06
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2702241-9
ISSN	2050-7518 ; 2050-750X
ISSN (online)	2050-7518
ISSN	2050-750X
DOI	10.1039/d2tb00379a
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Differential Protein Expression among Two Different Ovine ARDS Phenotypes-A Preclinical Randomized Study.

Wildi, Karin / Bouquet, Mahe / Ainola, Carmen / Livingstone, Samantha / Colombo, Sebastiano Maria / Heinsar, Silver / Sato, Noriko / Sato, Kei / Wilson, Emily / Abbate, Gabriella / Passmore, Margaret R / Hyslop, Kieran / Liu, Keibun / Li Bassi, Gianluigi / Suen, Jacky Y / Fraser, John F

Metabolites

2022 Band 12, Heft 7

Abstract: Despite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery of inflammatory subphenotypes in human ARDS provides a new approach to study the disease. ... ...

Abstract	Despite decades of comprehensive research, Acute Respiratory Distress Syndrome (ARDS) remains a disease with high mortality and morbidity worldwide. The discovery of inflammatory subphenotypes in human ARDS provides a new approach to study the disease. In two different ovine ARDS lung injury models, one induced by additional endotoxin infusion (phenotype 2), mimicking some key features as described in the human hyperinflammatory group, we aim to describe protein expression among the two different ovine models. Nine animals on mechanical ventilation were included in this study and were randomized into (a) phenotype 1,
Sprache	Englisch
Erscheinungsdatum	2022-07-15
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ZDB-ID	2662251-8
ISSN	2218-1989
ISSN	2218-1989
DOI	10.3390/metabo12070655
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Extracorporeal Membrane Oxygenation-Induced Hemolysis: An In Vitro Study to Appraise Causative Factors.

Chan, Chris Hoi Houng / Ki, Katrina K / Zhang, Meili / Asnicar, Cooper / Cho, Hwa Jin / Ainola, Carmen / Bouquet, Mahe / Heinsar, Silver / Pauls, Jo Philipp / Li Bassi, Gianluigi / Suen, Jacky / Fraser, John F

Membranes

2021 Band 11, Heft 5

Abstract: In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study ... ...

Abstract	In vitro hemolysis testing is commonly used to determine hemocompatibility of ExtraCorporeal Membrane Oxygenation (ECMO). However, poor reproducibility remains a challenging problem, due to several unidentified influencing factors. The present study investigated potential factors, such as flow rates, the use of anticoagulants, and gender of blood donors, which could play a role in hemolysis. Fresh human whole blood was anticoagulated with either citrate (n = 6) or heparin (n = 12; 6 female and 6 male blood donors). Blood was then circulated for 360 min at 4 L/min or 1.5 L/min. Regardless of flow rate conditions, hemolysis remained unchanged over time in citrated blood, but significantly increased after 240 min circulation in heparinized blood (
Sprache	Englisch
Erscheinungsdatum	2021-04-25
Erscheinungsland	Switzerland
Dokumenttyp	Journal Article
ISSN	2077-0375
ISSN	2077-0375
DOI	10.3390/membranes11050313
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel ; Online: Application of anti-inflammatory treatment in two different ovine Acute Respiratory Distress Syndrome injury models: a preclinical randomized intervention study.

Scientific reports

2023 Band 13, Heft 1, Seite(n) 17986

Abstract: Whilst the presence of 2 subphenotypes among the heterogenous Acute Respiratory Distress Syndrome (ARDS) population is becoming clinically accepted, subphenotype-specific treatment efficacy has yet to be prospectively tested. We investigated anti- ... ...

Abstract	Whilst the presence of 2 subphenotypes among the heterogenous Acute Respiratory Distress Syndrome (ARDS) population is becoming clinically accepted, subphenotype-specific treatment efficacy has yet to be prospectively tested. We investigated anti-inflammatory treatment in different ARDS models in sheep, previously shown similarities to human ARDS subphenotypes, in a preclinical, randomized, blinded study. Thirty anesthetized sheep were studied up to 48 h and randomized into: (a) OA: oleic acid (n = 15) and (b) OA-LPS: oleic acid and subsequent lipopolysaccharide (n = 15) to achieve a PaO
Mesh-Begriff(e)	Animals ; Anti-Inflammatory Agents/pharmacology ; Anti-Inflammatory Agents/therapeutic use ; Erythromycin/therapeutic use ; Methylprednisolone/pharmacology ; Methylprednisolone/therapeutic use ; Oleic Acid/therapeutic use ; Respiration ; Respiratory Distress Syndrome ; Sheep ; Random Allocation ; Disease Models, Animal
Chemische Substanzen	Anti-Inflammatory Agents ; Erythromycin (63937KV33D) ; Methylprednisolone (X4W7ZR7023) ; Oleic Acid (2UMI9U37CP)
Sprache	Englisch
Erscheinungsdatum	2023-10-20
Erscheinungsland	England
Dokumenttyp	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-023-45081-8
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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Artikel: Recovery of organ-specific tissue oxygen delivery at restrictive transfusion thresholds after fluid treatment in ovine haemorrhagic shock.

Dyer, Wayne B / Simonova, Gabriela / Chiaretti, Sara / Bouquet, Mahe / Wellburn, Rebecca / Heinsar, Silver / Ainola, Carmen / Wildi, Karin / Sato, Kei / Livingstone, Samantha / Suen, Jacky Y / Irving, David O / Tung, John-Paul / Li Bassi, Gianluigi / Fraser, John F

Intensive care medicine experimental

2022 Band 10, Heft 1, Seite(n) 12

Abstract: Background: Fluid resuscitation is the standard treatment to restore circulating blood volume and pressure after massive haemorrhage and shock. Packed red blood cells (PRBC) are transfused to restore haemoglobin levels. Restoration of microcirculatory ... ...

Abstract	Background: Fluid resuscitation is the standard treatment to restore circulating blood volume and pressure after massive haemorrhage and shock. Packed red blood cells (PRBC) are transfused to restore haemoglobin levels. Restoration of microcirculatory flow and tissue oxygen delivery is critical for organ and patient survival, but these parameters are infrequently measured. Patient Blood Management is a multidisciplinary approach to manage and conserve a patient's own blood, directing treatment options based on broad clinical assessment beyond haemoglobin alone, for which tissue perfusion and oxygenation could be useful. Our aim was to assess utility of non-invasive tissue-specific measures to compare PRBC transfusion with novel crystalloid treatments for haemorrhagic shock. Methods: A model of severe haemorrhagic shock was developed in an intensive care setting, with controlled haemorrhage in sheep according to pressure (mean arterial pressure 30-40 mmHg) and oxygen debt (lactate > 4 mM) targets. We compared PRBC transfusion to fluid resuscitation with either PlasmaLyte or a novel crystalloid. Efficacy was assessed according to recovery of haemodynamic parameters and non-invasive measures of sublingual microcirculatory flow, regional tissue oxygen saturation, repayment of oxygen debt (arterial lactate), and a panel of inflammatory and organ function markers. Invasive measurements of tissue perfusion, oxygen tension and lactate levels were performed in brain, kidney, liver, and skeletal muscle. Outcomes were assessed during 4 h treatment and post-mortem, and analysed by one- and two-way ANOVA. Results: Each treatment restored haemodynamic and tissue oxygen delivery parameters equivalently (p > 0.05), despite haemodilution after crystalloid infusion to haemoglobin concentrations below 70 g/L (p < 0.001). Recovery of vital organ-specific perfusion and oxygen tension commenced shortly before non-invasive measures improved. Lactate declined in all tissues and correlated with arterial lactate levels (p < 0.0001). The novel crystalloid supported rapid peripheral vasodilation (p = 0.014) and tended to achieve tissue oxygen delivery targets earlier. PRBC supported earlier renal oxygen delivery (p = 0.012) but delayed peripheral perfusion (p = 0.034). Conclusions: Crystalloids supported vital organ oxygen delivery after massive haemorrhage, despite haemodilution to < 70 g/L, confirming that restrictive transfusion thresholds are appropriate to support oxygen delivery. Non-invasive tissue perfusion and oximetry technologies merit further clinical appraisal to guide treatment for massive haemorrhage in the context of Patient Blood Management.
Sprache	Englisch
Erscheinungsdatum	2022-04-04
Erscheinungsland	Germany
Dokumenttyp	Journal Article
ZDB-ID	2740385-3
ISSN	2197-425X
ISSN	2197-425X
DOI	10.1186/s40635-022-00439-6
Datenquelle	MEDical Literature Analysis and Retrieval System OnLINE

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