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Article: Peptostreptococcus faecalis sp. nov., new bacterial species isolated from healthy indigenous congolese volunteer

Mekhalif, Fatima / Zgheib, Rita / Akiana, Jean / Bilen, Melhem / Ndombe, Geor Mongo / Fenollar, Florence / Fournier, Pierre-Edouard / Raoult, Didier / Alibar, Stéphane / Mediannikov, Oleg / Lo, Cheikh Ibrahima

Heliyon. 2022 Mar., v. 8, no. 3

2022

Abstract: The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308ᵀ from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308ᵀ is a Gram-positive coccus ... ...

Abstract	The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308ᵀ from a stool sample of a healthy indigenous Congolese volunteer. Strain Marseille-P4308ᵀ is a Gram-positive coccus shaped bacterium that optimally grows at 37 °C. The 16S rRNA gene sequence of the strain has a 96.2% sequence similarity to Peptostreptococcus anaerobius strain NCTC 11460ᵀ (GenBank accession number: NR_042847.1). In addition, the average nucleotide identity of strain Marseille-P4308ᵀ with its closest related species was 71.1%, which was far below the recommended threshold (>95–96%). The genome of the strain Marseille-P4308ᵀ has a length of 2.14 Mbp with G + C content of 30.4 mol%. Based on phenotypic, biochemical, genomic and phylogenetic analysis, strain Marseille-P4308ᵀ (= CSUR P4308 = CECT 9960) clearly appears to be a new species for which the name Peptostreptococcus faecalis sp. nov., is proposed.
Keywords	Peptostreptococcus anaerobius ; bacteria ; feces ; genome ; genomics ; new species ; nucleotide sequences ; phenotype ; phylogeny ; sequence homology
Language	English
Dates of publication	2022-03
Publishing place	Elsevier Ltd
Document type	Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e09102
Database	NAL-Catalogue (AGRICOLA)

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Article: Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations.

Menu, Estelle / Davoust, Bernard / Mediannikov, Oleg / Akiana, Jean / Mulot, Baptiste / Diatta, Georges / Levasseur, Anthony / Ranque, Stéphane / Raoult, Didier / Bittar, Fadi

Pathogens (Basel, Switzerland)

2021 Volume 10, Issue 3

Abstract: Non-human primate populations act as potential reservoirs for human pathogens, including viruses, bacteria and parasites, which can lead to zoonotic infections. Furthermore, intestinal microorganisms may be pathogenic organisms to both non-human primates ...

Abstract	Non-human primate populations act as potential reservoirs for human pathogens, including viruses, bacteria and parasites, which can lead to zoonotic infections. Furthermore, intestinal microorganisms may be pathogenic organisms to both non-human primates and humans. It is, therefore, essential to study the prevalence of these infectious agents in captive and wild non-human primates. This study aimed at showing the prevalence of the most frequently encountered human enteric protozoa in non-human primate populations based on qPCR detection. The three populations studied were common chimpanzees (
Language	English
Publishing date	2021-03-02
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10030280
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Occurrence of Ten Protozoan Enteric Pathogens in Three Non-Human Primate Populations

Menu, Estelle / Davoust, Bernard / Mediannikov, Oleg / Akiana, Jean / Mulot, Baptiste / Diatta, Georges / Levasseur, Anthony / Ranque, Stéphane / Raoult, Didier / Bittar, Fadi

Pathogens. 2021 Mar. 02, v. 10, no. 3

2021

Abstract	Non-human primate populations act as potential reservoirs for human pathogens, including viruses, bacteria and parasites, which can lead to zoonotic infections. Furthermore, intestinal microorganisms may be pathogenic organisms to both non-human primates and humans. It is, therefore, essential to study the prevalence of these infectious agents in captive and wild non-human primates. This study aimed at showing the prevalence of the most frequently encountered human enteric protozoa in non-human primate populations based on qPCR detection. The three populations studied were common chimpanzees (Pan troglodytes) in Senegal and gorillas (Gorilla gorilla) in the Republic of the Congo and in the Beauval Zoo (France). Blastocystis spp. were mainly found, with an occurrence close to 100%, followed by Balantidiumcoli (23.7%), Giardiaintestinalis (7.9%), Encephalitozoonintestinalis (1.3%) and Dientamoebafragilis (0.2%). None of the following protozoa were detected: Entamoebahistolytica, Enterocytozoonbieneusi, Cryptosporidiumparvum, C. hominis, Cyclosporacayetanensis or Cystoisosporabelli. As chimpanzees and gorillas are genetically close to humans, it is important to monitor them frequently against different pathogens to protect these endangered species and to assess potential zoonotic transmissions to humans.
Keywords	Blastocystis ; Gorilla gorilla ; Protozoa ; endangered species ; humans ; intestines ; zoos ; France ; Republic of the Congo ; Senegal
Language	English
Dates of publication	2021-0302
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ZDB-ID	2695572-6
ISSN	2076-0817
ISSN	2076-0817
DOI	10.3390/pathogens10030280
Database	NAL-Catalogue (AGRICOLA)

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Article: Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces

Sereme, Youssouf / Zarza, Sandra Madariaga / Medkour, Hacène / Amona, Inestin / Fenollar, Florence / Akiana, Jean / Mezouar, Soraya / Orain, Nicolas / Vitte, Joana / Davoust, Bernard / Raoult, Didier / Mediannikov, Oleg

Microorganisms. 2021 Apr. 12, v. 9, no. 4

2021

Abstract: Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve ( ... ...

Abstract	Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve (Republic of Congo) the presence of a new Enterovirus C (Ibou002) in a male gorilla that was put away because of clinical symptoms of facial paralysis. This new virus, isolated was from the stool samples of this gorilla but also from the excrement of an eco-guardian, is very similar to Coxsackievirus (EV-C99) as well as poliovirus 1 and 2. We hypothesised that these symptoms might be due to poliovirus infection. To test our hypothesis, we developed and optimised a non-invasive immunoassay for the detection of Enterovirus-specific antibodies in gorilla faeces that could be useful for routine serosurveillance in such cases. Methods: In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts from 16 gorilla and 3 humans, to detect specific antibodies by western blot and ELISA. Results: First, total immunoglobulins were detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. Conclusion: We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive approach to detect microorganism-specific immunoglobulins from faecal samples opens numerous prospects for application in zoonotic infectious diseases and could revolutionise the screening of animals for important emerging infections, such as Ebola fever, rabies and coronavirus infections.
Keywords	Enterovirus C ; Gorilla ; Western blotting ; antigens ; conservation areas ; feces ; fever ; freeze drying ; humans ; immunoglobulins ; males ; paralysis ; rabies ; serology ; solubilization ; vaccines ; viruses ; Republic of the Congo
Language	English
Dates of publication	2021-0412
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-AP-2-clean
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9040810
Database	NAL-Catalogue (AGRICOLA)

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Article: Peptostreptococcus faecalis

Heliyon

2022 Volume 8, Issue 3, Page(s) e09102

Abstract: The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille- ... ...

Abstract	The Microbial Culturomics Project aiming to discover several bacterial species made it possible to isolate the strain Marseille-P4308
Language	English
Publishing date	2022-03-14
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2022.e09102
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Stool Serology: Development of a Non-Invasive Immunological Method for the Detection of Enterovirus-Specific Antibodies in Congo Gorilla Faeces.

Microorganisms

2021 Volume 9, Issue 4

Abstract	Background: The incidence of poliovirus has been significantly reduced by as much as 99.9% globally. Alongside this, however, vaccine-associated paralytic poliomyelitis has emerged. Previously, our team reported in the Lésio-Louna-Léfini Nature Reserve (Republic of Congo) the presence of a new Methods: In order to assess the potential role of poliovirus infection, we have developed and optimised a protocol, based on the lyophilisation and solubilisation of small volumes of stool extracts from 16 gorilla and 3 humans, to detect specific antibodies by western blot and ELISA. Results: First, total immunoglobulins were detected in the concentrated stool extracts. Specific antibodies were then detected in 4/16 gorilla samples and 2/3 human samples by western blot using both the polio vaccine antigen and the Ibou002 antigen and by ELISA using the polio vaccine antigen. Humoral responses were greater with the Ibou002 antigen. Conclusion: We therefore suggest that this recombinant virus could lead to a polio-like disease in the endangered western lowland gorilla. The development of a non-invasive approach to detect microorganism-specific immunoglobulins from faecal samples opens numerous prospects for application in zoonotic infectious diseases and could revolutionise the screening of animals for important emerging infections, such as Ebola fever, rabies and coronavirus infections.
Language	English
Publishing date	2021-04-12
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms9040810
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Enteroviruses from Humans and Great Apes in the Republic of Congo: Recombination within Enterovirus C Serotypes

Amona, Inestin / Medkour, Hacène / Akiana, Jean / Davoust, Bernard / Tall, Mamadou Lamine / Grimaldier, Clio / Gazin, Celine / Zandotti, Christine / Levasseur, Anthony / Scola, Bernard La / Raoult, Didier / Fenollar, Florence / Banga-Mboko, Henri / Mediannikov, Oleg

Microorganisms. 2020 Nov. 13, v. 8, no. 11

2020

Abstract: Enteroviruses (EVs) are viruses of the family Picornaviridae that cause mild to severe infections in humans and in several animal species, including non-human primates (NHPs). We conducted a survey and characterization of enteroviruses circulating ... ...

Abstract	Enteroviruses (EVs) are viruses of the family Picornaviridae that cause mild to severe infections in humans and in several animal species, including non-human primates (NHPs). We conducted a survey and characterization of enteroviruses circulating between humans and great apes in the Congo. Fecal samples (N = 24) of gorillas and chimpanzees living close to or distant from humans in three Congolese parks were collected, as well as from healthy humans (N = 38) living around and within these parks. Enteroviruses were detected in 29.4% of gorilla and 13.15% of human feces, including wild and human-habituated gorillas, local humans and eco-guards. Two identical strains were isolated from two humans coming from two remote regions. Their genomes were similar and all genes showed their close similarity to coxsackieviruses, except for the 3C, 3D and 5′-UTR regions, where they were most similar to poliovirus 1 and 2, suggesting recombination. Recombination events were found between these strains, poliovirus 1 and 2 and EV-C99. It is possible that the same EV-C species circulated in both humans and apes in different regions in the Congo, which must be confirmed in other investigations. In addition, other studies are needed to further investigate the circulation and genetic diversity of enteroviruses in the great ape population, to draw a definitive conclusion on the different species and types of enteroviruses circulating in the Republic of Congo.
Keywords	Enterovirus C ; Gorilla ; Pan troglodytes ; feces ; genes ; genetic variation ; humans ; parks ; population ; serotypes ; surveys ; viruses ; Republic of the Congo
Language	English
Dates of publication	2020-1113
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
Note	NAL-light
ZDB-ID	2720891-6
ISSN	2076-2607
ISSN	2076-2607
DOI	10.3390/microorganisms8111779
Database	NAL-Catalogue (AGRICOLA)

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Article: Adenovirus Infections in African Humans and Wild Non-Human Primates: Great Diversity and Cross-Species Transmission

Medkour, Hacène / Amona, Inestin / Akiana, Jean / Davoust, Bernard / Bitam, Idir / Levasseur, Anthony / Tall, Mamadou Lamine / Diatta, Georges / Sokhna, Cheikh / Hernandez-Aguilar, Raquel Adriana / Barciela, Amanda / Gorsane, Slim / La Scola, Bernard / Raoult, Didier / Fenollar, Florence / Mediannikov, Oleg

Viruses. 2020 June 18, v. 12, no. 6

2020

Abstract: Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The ... ...

Abstract	Non-human primates (NHPs) are known hosts for adenoviruses (AdVs), so there is the possibility of the zoonotic or cross-species transmission of AdVs. As with humans, AdV infections in animals can cause diseases that range from asymptomatic to fatal. The aim of this study was to investigate the occurrence and diversity of AdVs in: (i) fecal samples of apes and monkeys from different African countries (Republic of Congo, Senegal, Djibouti and Algeria), (ii) stool of humans living near gorillas in the Republic of Congo, in order to explore the potential zoonotic risks. Samples were screened by real-time and standard PCRs, followed by the sequencing of the partial DNA polymerase gene in order to identify the AdV species. The prevalence was 3.3 folds higher in NHPs than in humans. More than 1/3 (35.8%) of the NHPs and 1/10 (10.5%) of the humans excreted AdVs in their feces. The positive rate was high in great apes (46%), with a maximum of 54.2% in chimpanzees (Pan troglodytes) and 35.9% in gorillas (Gorilla gorilla), followed by monkeys (25.6%), with 27.5% in Barbary macaques (Macaca sylvanus) and 23.1% in baboons (seven Papio papio and six Papio hamadryas). No green monkeys (Chlorocebus sabaeus) were found to be positive for AdVs. The AdVs detected in NHPs were members of Human mastadenovirus E (HAdV-E), HAdV-C or HAdV-B, and those in the humans belonged to HAdV-C or HAdV-D. HAdV-C members were detected in both gorillas and humans, with evidence of zoonotic transmission since phylogenetic analysis revealed that gorilla AdVs belonging to HAdV-C were genetically identical to strains detected in humans who had been living around gorillas, and, inversely, a HAdV-C member HAdV type was detected in gorillas. This confirms the gorilla-to-human transmission of adenovirus. which has been reported previously. In addition, HAdV-E members, the most often detected here, are widely distributed among NHP species regardless of their origin, i.e., HAdV-E members seem to lack host specificity. Virus isolation was successful from a human sample and the strain of the Mbo024 genome, of 35 kb, that was identified as belonging to HAdV-D, exhibited close identity to HAdV-D members for all genes. This study provides information on the AdVs that infect African NHPs and the human populations living nearby, with an evident zoonotic transmission. It is likely that AdVs crossed the species barrier between different NHP species (especially HAdV-E members), between NHPs and humans (especially HAdV-C), but also between humans, NHPs and other animal species.
Keywords	Adenoviridae infections ; Chlorocebus sabaeus ; DNA-directed DNA polymerase ; Gorilla gorilla ; Human mastadenovirus E ; Macaca sylvanus ; Pan troglodytes ; Papio hamadryas ; Papio papio ; animal viruses ; feces ; genes ; host specificity ; hosts ; human population ; humans ; monkeys ; phylogeny ; polymerase chain reaction ; risk ; viruses ; zoonoses ; Algeria ; Djibouti ; Republic of the Congo ; Senegal
Language	English
Dates of publication	2020-0618
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article
ZDB-ID	2516098-9
ISSN	1999-4915
ISSN	1999-4915
DOI	10.3390/v12060657
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: High prevalence of Plasmodium falciparum antimalarial drug resistance markers in isolates from asymptomatic patients from the Republic of the Congo between 2010 and 2015.

Voumbo-Matoumona, Dominique Fatima / Akiana, Jean / Madamet, Marylin / Kouna, Lady Charlène / Lekana-Douki, Jean Bernard / Pradines, Bruno

Journal of global antimicrobial resistance

2018 Volume 14, Page(s) 277–283

Abstract: Objectives: This study investigated the prevalence of haplotypes of the Pfdhps, Pfdhfr, Pfcrt, Pfmdr1 and PfK13 resistance markers in isolates from asymptomatic patients from the Republic of the Congo following implementation of artemisinin based- ... ...

Abstract	Objectives: This study investigated the prevalence of haplotypes of the Pfdhps, Pfdhfr, Pfcrt, Pfmdr1 and PfK13 resistance markers in isolates from asymptomatic patients from the Republic of the Congo following implementation of artemisinin based-combination therapy (ACT). Methods: Peripheral blood was collected from asymptomatic children in 2010 and 2015 from Brazzaville in the south and in 2013 in the north of the Congo. Genotypes of Pfmdr1, Pfcrt, Pfdhps, Pfdhfr and PfK13 were assessed by PCR. Results: Children from 2010 were younger than those from 2015 (mean age 5.38 years vs. 8.67 years; P=0.003). The main Pfcrt haplotype was the wild-type CVMNK (84.85%) in 2010, whereas the mutant CVIET (61.64%) predominated in 2015 (P<0.001). In the north, 45.00% of samples were CVMNK and 10.00% were CVIET. Other samples harboured new haplotypes in the country or mixed alleles. No significant difference in Pfmdr1 haplotypes was observed in 2010 and 2015 and the main haplotypes were NYD and NFD (30.56% vs. 28.57% and 61.11% vs. 42.86% for 2010 and 2015, respectively). In the south, the Pfdhps haplotypes observed were AAKAA, AGKAA, SGKAA and SGEGA (87.50% vs. 0%, 12.50% vs. 33.33%, 0% vs. 33.33% and 0% vs. 33.33% for 2010 and 2015, respectively). For Pfdhfr, the IRNI haplotype was most prevalent (85.71% for 2010, 87.50% for 2013 and 100% for 2015). No PfK13 mutations were found. Conclusions: Monitoring the efficacy of ACT and intermittent preventive treatment with sulfadoxine-pyrimethamine is necessary to ensure an epidemiological survey of asymptomatic malaria.
MeSH term(s)	Adolescent ; Adult ; Antimalarials/pharmacology ; Child ; Child, Preschool ; Congo/epidemiology ; Cross-Sectional Studies ; Drug Resistance ; Female ; Haplotypes ; Humans ; Infant ; Male ; Middle Aged ; Plasmodium falciparum/drug effects ; Plasmodium falciparum/genetics ; Plasmodium falciparum/isolation & purification ; Polymorphism, Single Nucleotide ; Prevalence ; Protozoan Proteins/genetics ; Sequence Analysis, DNA/methods ; Young Adult
Chemical Substances	Antimalarials ; Protozoan Proteins
Language	English
Publishing date	2018-08-16
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2710046-7
ISSN	2213-7173 ; 2213-7165
ISSN (online)	2213-7173
ISSN	2213-7165
DOI	10.1016/j.jgar.2018.08.003
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article: Detection of novel RNA viruses from free-living gorillas, Republic of the Congo: genetic diversity of picobirnaviruses

Duraisamy, Raja / Akiana, Jean / Davoust, Bernard / Mediannikov, Oleg / Michelle, Caroline / Robert, Catherine / Parra, Henri-Joseph / Raoult, Didier / Biagini, Philippe / Desnues, Christelle

Virus genes. 2018 Apr., v. 54, no. 2

2018

Abstract: Most of the emerging infectious diseases reported so far originated in wildlife. Therefore, virological surveillance of animals and particularly great apes is of great interest to establish the repertory of viruses associated with healthy hosts. This ... ...

Abstract	Most of the emerging infectious diseases reported so far originated in wildlife. Therefore, virological surveillance of animals and particularly great apes is of great interest to establish the repertory of viruses associated with healthy hosts. This will further help to identify the emergence of new viruses and predict the possibility of interspecies transmission. In this study, we performed shotgun viral metagenomics on stool samples collected from seventeen free-living wild gorillas from the Republic of the Congo. The analysis revealed the presence of novel RNA viruses (picobirnaviruses, partitivirus, and Picornavirales (posa-like and dicistrovirus-like viruses)). Among these, picobirnavirus-related sequences were abundantly covered in the stools. Based on genetic variations both in capsid and RdRp proteins of picobirnaviruses, at least 96 variants were identified and most of them were novel. Among the 96, 22 variants had a nearly complete genome or segment. A comprehensive sequence analysis identified a potential new genogroup/genetic cluster and the presence of a short linear amino acid motif (ExxRxNxxxE) in a hypothetical protein. The sequence analysis of posa-like virus and dicistrovirus showed that these two viruses were novel members in the respective viral families. In conclusion, the identification of novel RNA viruses and their genetic diversity increases our knowledge about viruses that are associated with stools of wild gorillas and contributes to the initiatives in the search for potential emerging zoonotic viruses.
Keywords	sequence analysis ; proteins ; wildlife ; Picornavirales ; animals ; monitoring ; capsid ; Partitiviridae ; genome ; emerging diseases ; hosts ; disease transmission ; Pongidae ; feces ; metagenomics ; viruses ; genetic variation ; Picobirnavirus ; Gorilla ; Republic of the Congo ; covid19
Language	English
Dates of publication	2018-04
Size	p. 256-271.
Publishing place	Springer US
Document type	Article
Note	2019-12-06
ZDB-ID	639496-6
ISSN	1572-994X ; 0920-8569
ISSN (online)	1572-994X
ISSN	0920-8569
DOI	10.1007/s11262-018-1543-6
Database	NAL-Catalogue (AGRICOLA)

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