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Article ; Online: Effects of pulsed electrical stimulation on α-smooth muscle actin and type I collagen expression in human dermal fibroblasts.

Urabe, Hiroya / Akimoto, Ryuji / Kamiya, Shohei / Hosoki, Katsu / Ichikawa, Hideyuki / Nishiyama, Toshio

Bioscience, biotechnology, and biochemistry

2024 Volume 88, Issue 5, Page(s) 522–528

Abstract: Pulsed electrical stimulation (PES) is known to affect cellular activities. We previously found PES to human dermal fibroblasts (HFs) promoted platelet-derived growth factor subunit A (PDGFA) gene expression, which enhanced proliferation. In this study, ... ...

Abstract	Pulsed electrical stimulation (PES) is known to affect cellular activities. We previously found PES to human dermal fibroblasts (HFs) promoted platelet-derived growth factor subunit A (PDGFA) gene expression, which enhanced proliferation. In this study, we investigated PES effects on fibroblast collagen production and differentiation into myofibroblasts. HFs were electrically stimulated at 4800 Hz and 5 V for 60 min. Imatinib, a specific inhibitor of PDGF receptors, was treated before PES. After 6 h of PES, PDGFA, α-smooth muscle actin (α-SMA), and collagen type I α1 chain gene expressions were upregulated in PES group. Imatinib suppressed the promoted expression except for PDGFA. Immunofluorescence staining and enzyme-linked immunosorbent assay showed the production of α-SMA and collagen I was enhanced in PES group but suppressed in PES + imatinib group at 48 h after PES. Therefore, PES promotes the production of α-SMA and collagen I in fibroblasts, which is triggered by PDGFA that is upregulated early after PES.
MeSH term(s)	Humans ; Collagen Type I/metabolism ; Collagen Type I/genetics ; Actins/metabolism ; Actins/genetics ; Fibroblasts/metabolism ; Fibroblasts/drug effects ; Platelet-Derived Growth Factor/metabolism ; Electric Stimulation ; Imatinib Mesylate/pharmacology ; Cell Differentiation/drug effects ; Skin/metabolism ; Skin/cytology ; Cells, Cultured ; Gene Expression Regulation/drug effects ; Dermis/cytology ; Dermis/metabolism ; Receptors, Platelet-Derived Growth Factor/metabolism ; Receptors, Platelet-Derived Growth Factor/genetics ; Up-Regulation
Chemical Substances	Collagen Type I ; Actins ; Platelet-Derived Growth Factor ; ACTA2 protein, human ; Imatinib Mesylate (8A1O1M485B) ; platelet-derived growth factor A ; Receptors, Platelet-Derived Growth Factor (EC 2.7.10.1)
Language	English
Publishing date	2024-02-09
Publishing country	England
Document type	Journal Article
ZDB-ID	1106450-x
ISSN	1347-6947 ; 0916-8451
ISSN (online)	1347-6947
ISSN	0916-8451
DOI	10.1093/bbb/zbae017
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Zs.A 4647: Show issues

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Article ; Online: Belt electrode tetanus muscle stimulation reduces denervation-induced atrophy of rat multiple skeletal muscle groups.

Uno, Hiroyuki / Kamiya, Shohei / Akimoto, Ryuji / Hosoki, Katsu / Tadano, Shunta / Isemura, Mako / Kouzaki, Karina / Tamura, Yuki / Kotani, Takaya / Nakazato, Koichi

Scientific reports

2024 Volume 14, Issue 1, Page(s) 5848

Abstract: Belt electrode-skeletal muscle electrical stimulation (B-SES) involves the use of belt-shaped electrodes to contract multiple muscle groups simultaneously. Twitch contractions have been demonstrated to protect against denervation-induced muscle atrophy ... ...

Abstract	Belt electrode-skeletal muscle electrical stimulation (B-SES) involves the use of belt-shaped electrodes to contract multiple muscle groups simultaneously. Twitch contractions have been demonstrated to protect against denervation-induced muscle atrophy in rats, possibly through mitochondrial biosynthesis. This study examined whether inducing tetanus contractions with B-SES suppresses muscle atrophy and identified the underlying molecular mechanisms. We evaluated the effects of acute (60 Hz, 5 min) and chronic (60 Hz, 5 min, every alternate day for one week) B-SES on the tibialis anterior (TA) and gastrocnemius (GAS) muscles in Sprague-Dawley rats using belt electrodes attached to both ankle joints. After acute stimulation, a significant decrease in the glycogen content was observed in the left and right TA and GAS, suggesting that B-SES causes simultaneous contractions in multiple muscle groups. B-SES enhanced p70S6K phosphorylation, an indicator of the mechanistic target of rapamycin complex 1 activity. During chronic stimulations, rats were divided into control (CONT), denervation-induced atrophy (DEN), and DEN + electrically stimulated with B-SES (DEN + ES) groups. After seven days of treatment, the wet weight (n = 8-11 for each group) and muscle fiber cross-sectional area (CSA, n = 6 for each group) of the TA and GAS muscles were reduced in the DEN and DEN + ES groups compared with that in the CON group. The DEN + ES group showed significantly higher muscle weight and CSA than those in the DEN group. Although RNA-seq and pathway analysis suggested that mitochondrial biogenesis is a critical event in this phenomenon, mitochondrial content showed no difference. In contrast, ribosomal RNA 28S and 18S (n = 6) levels in the DEN + ES group were higher than those in the DEN group, even though RNA-seq showed that the ribosome biogenesis pathway was reduced by electrical stimulation. The mRNA levels of the muscle proteolytic molecules atrogin-1 and MuRF1 were significantly higher in DEN than those in CONT. However, they were more suppressed in DEN + ES than those in DEN. In conclusion, tetanic electrical stimulation of both ankles using belt electrodes effectively reduced denervation-induced atrophy in multiple muscle groups. Furthermore, ribosomal biosynthesis plays a vital role in this phenomenon.
MeSH term(s)	Rats ; Animals ; Tetanus ; Rats, Sprague-Dawley ; Muscle, Skeletal/metabolism ; Muscular Atrophy/etiology ; Muscular Atrophy/prevention & control ; Electric Stimulation ; Denervation ; Electrodes
Language	English
Publishing date	2024-03-11
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-56382-x
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Article: Pulsed electrical stimulation and amino acid derivatives promote collagen gene expression in human dermal fibroblasts.

Urabe, Hiroya / Akimoto, Ryuji / Kamiya, Shohei / Hosoki, Katsu / Ichikawa, Hideyuki / Nishiyama, Toshio

Cytotechnology

2023 Volume 76, Issue 1, Page(s) 139–151

Abstract: Several collagen types are important for maintaining skin structure and function. Previous reports show that l-hydroxyproline (Hyp), N-acetyl-l-hydroxyproline (AHyp), and l-alanyl-l-glutamine (Aln-Gln) are biological active substances with collagen ... ...

Abstract	Several collagen types are important for maintaining skin structure and function. Previous reports show that l-hydroxyproline (Hyp), N-acetyl-l-hydroxyproline (AHyp), and l-alanyl-l-glutamine (Aln-Gln) are biological active substances with collagen synthesis-promoting effects. In this study, we combined the promotive effects of pulsed electrical stimulation (PES) with three amino acid derivatives in human dermal fibroblasts. Fibroblasts were exposed to PES with a 4,800 Hz pulse frequency and a voltage at 1 or 5 V for 15 min. The gene expression of type I and III collagen (fibrillar collagen), type IV and VII collagen (basement membrane collagen and anchoring fibril collagen) were measured by RT-PCR 48 h after PES. PES alone promoted the expression of
Language	English
Publishing date	2023-11-09
Publishing country	United States
Document type	Journal Article
ZDB-ID	1035772-5
ISSN	0920-9069
ISSN	0920-9069
DOI	10.1007/s10616-023-00604-z
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Zs.A 2604: Show issues

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Article ; Online: A Case of Nail Yellow Discoloration due to Topical Treatment of Onychomycosis with Luliconazole 5% Nail Solution.

Sato, Tomotaka / Fukada, Norihito / Kinjo, Akihiko / Akimoto, Ryuta / Kobayashi, Hisashi / Fukuzumi, Satoshi

Medical mycology journal

2023 Volume 64, Issue 2, Page(s) 45–47

Abstract: A woman in her 70s had onychomycosis that was treated with topical luliconazole solution. Her nails changed color to yellow due to the treatment and exposure to sunlight. Avoidance of sunlight and continuous application of luliconazole resolved the ... ...

Abstract	A woman in her 70s had onychomycosis that was treated with topical luliconazole solution. Her nails changed color to yellow due to the treatment and exposure to sunlight. Avoidance of sunlight and continuous application of luliconazole resolved the discoloration and were effective for the treatment of onychomycosis one year after the first visit.
MeSH term(s)	Humans ; Female ; Onychomycosis/drug therapy ; Nails ; Antifungal Agents ; Imidazoles/adverse effects ; Administration, Topical
Chemical Substances	luliconazole (RE91AN4S8G) ; Antifungal Agents ; Imidazoles
Language	English
Publishing date	2023-05-31
Publishing country	Japan
Document type	Case Reports ; Journal Article
ZDB-ID	2628906-4
ISSN	1882-0476 ; 2186-165X ; 2185-6486 ; 0916-4804
ISSN (online)	1882-0476 ; 2186-165X
ISSN	2185-6486 ; 0916-4804
DOI	10.3314/mmj.22-00030
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Zs.A 1971: Show issues

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Article ; Online: Low-frequency electrical stimulation of bilateral hind legs by belt electrodes is effective for preventing denervation-induced atrophies in multiple skeletal muscle groups in rats.

Uno, Hiroyuki / Kamiya, Shohei / Akimoto, Ryuji / Hosoki, Katsu / Tadano, Shunta / Kouzaki, Karina / Tamura, Yuki / Kotani, Takaya / Isemura, Mako / Nakazato, Koichi

Scientific reports

2022 Volume 12, Issue 1, Page(s) 21275

Abstract: Belt electrode skeletal muscle electrical stimulation (B-SES) can simultaneously contract multiple muscle groups. Although the beneficial effects of B-SES in clinical situations have been elucidated, its molecular mechanism remains unknown. In this study, ...

Abstract	Belt electrode skeletal muscle electrical stimulation (B-SES) can simultaneously contract multiple muscle groups. Although the beneficial effects of B-SES in clinical situations have been elucidated, its molecular mechanism remains unknown. In this study, we developed a novel rodent B-SES ankle stimulation system to test whether low-frequency stimulation prevents denervation-induced muscle atrophy. Electrical stimulations (7‒8 Hz, 30 min) with ankle belt electrodes were applied to Sprague-Dawley rats daily for one week. All animals were assigned to the control (CONT), denervation-induced atrophy (DEN), and DEN + electrical stimulation (ES) groups. The tibialis anterior (TA) and gastrocnemius (GAS) muscles were used to examine the effect of ES treatment. After seven daily sessions of continuous stimulation, muscle wet weight (n = 8-11), and muscle fiber cross-sectional area (CSA, n = 4-6) of TA and GAS muscles were lower in DEN and DEN + ES than in CON. However, it was significantly higher in DEN than DEN + ES, showing that ES partially prevented muscle atrophy. PGC-1α, COX-IV, and citrate synthase activities (n = 6) were significantly higher in DEN + ES than in DEN. The mRNA levels of muscle proteolytic molecules, Atrogin-1 and Murf1, were significantly higher in DEN than in CONT, while B-SES significantly suppressed their expression (p < 0.05). In conclusion, low-frequency electrical stimulation of the bilateral ankles using belt electrodes (but not the pad electrodes) is effective in preventing denervation-induced atrophy in multiple muscles, which has not been observed with pad electrodes. Maintaining the mitochondrial quantity and enzyme activity by low-frequency electrical stimulation is key to suppressing muscle protein degradation.
MeSH term(s)	Rats ; Animals ; Rats, Sprague-Dawley ; Social Group ; Electric Stimulation ; Muscle, Skeletal ; Muscular Atrophy/prevention & control
Language	English
Publishing date	2022-12-08
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-25359-z
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Article ; Online: DGKζ depletion attenuates HIF-1α induction and SIRT1 expression, but enhances TAK1-mediated AMPKα phosphorylation under hypoxia.

Akimoto, Ryo / Tanaka, Toshiaki / Nakano, Tomoyuki / Hozumi, Yasukazu / Kawamae, Kaneyuki / Goto, Kaoru

Cellular signalling

2020 Volume 71, Page(s) 109618

Abstract: Cells cope with environmental changes through various mechanisms. Pathways involving HIF-1, SIRT1, and AMPK play major roles in energy homeostasis under stress conditions. Diacylglycerol kinase (DGK) constitutes an enzyme family that catalyzes conversion ...

Abstract	Cells cope with environmental changes through various mechanisms. Pathways involving HIF-1, SIRT1, and AMPK play major roles in energy homeostasis under stress conditions. Diacylglycerol kinase (DGK) constitutes an enzyme family that catalyzes conversion of diacylglycerol to phosphatidic acid. We reported earlier that energy depletion such as ischemia induces proteasomal degradation of DGKζ before cell death, suggesting involvement of DGKζ in energy homeostasis. This study examines how DGKζ depletion affects the regulation of HIF-1α, SIRT1, and AMPKα. Under hypoxia DGKζ depletion attenuates HIF-1α induction and SIRT1 expression, which might render cells vulnerable to energy stress. However, DGKζ depletion engenders enhanced AMPKα phosphorylation by upstream kinase TAK1 and an increase in intracellular ATP levels. Results suggest that DGKζ exerts a suppressive effect on TAK1 activity in the AMPK activation mechanism, and that DGKζ depletion might engender dysregulation of the AMPK-mediated energy sensor system.
MeSH term(s)	AMP-Activated Protein Kinases/metabolism ; Adenosine Triphosphate/metabolism ; Animals ; Cell Hypoxia ; Diacylglycerol Kinase/metabolism ; Enzyme Activation ; HeLa Cells ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/metabolism ; MAP Kinase Kinase Kinases/metabolism ; Mice, Inbred C57BL ; Phosphorylation ; Protein-Serine-Threonine Kinases/metabolism ; Sirtuin 1/metabolism
Chemical Substances	HIF1A protein, human ; Hypoxia-Inducible Factor 1, alpha Subunit ; Adenosine Triphosphate (8L70Q75FXE) ; STK11 protein, human (EC 2.7.1.-) ; Diacylglycerol Kinase (EC 2.7.1.107) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; MAP Kinase Kinase Kinases (EC 2.7.11.25) ; MAP kinase kinase kinase 7 (EC 2.7.11.25) ; AMP-Activated Protein Kinases (EC 2.7.11.31) ; Sirtuin 1 (EC 3.5.1.-)
Language	English
Publishing date	2020-03-26
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	1002702-6
ISSN	1873-3913 ; 0898-6568
ISSN (online)	1873-3913
ISSN	0898-6568
DOI	10.1016/j.cellsig.2020.109618
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Zs.A 2579: Show issues

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Article ; Online: Effects of pulsed electrical stimulation on growth factor gene expression and proliferation in human dermal fibroblasts.

Urabe, Hiroya / Akimoto, Ryuji / Kamiya, Shohei / Hosoki, Katsu / Ichikawa, Hideyuki / Nishiyama, Toshio

Molecular and cellular biochemistry

2020 Volume 476, Issue 1, Page(s) 361–368

Abstract: Human dermal fibroblast proliferation plays an important role in skin wound healing, and electrical stimulation (ES) promotes skin wound healing. Although the use of ES for skin wound healing has been investigated, the mechanism underlying the effects of ...

Abstract	Human dermal fibroblast proliferation plays an important role in skin wound healing, and electrical stimulation (ES) promotes skin wound healing. Although the use of ES for skin wound healing has been investigated, the mechanism underlying the effects of ES on cells is still unclear. This study examined the effects of pulsed electrical stimulation (PES) on human dermal fibroblasts. Normal adult human dermal fibroblasts were exposed to a frequency of 4800 Hz, voltage of 1-5 V, and PES exposure time of 15, 30, and 60 min. Dermal fibroblast proliferation and growth factor gene expression were investigated for 6-48 h post PES. Dermal fibroblast proliferation significantly increased from 24 to 48 h post PES at a voltage of 5 V and PES exposure time of 60 min. Under the same conditions, post PES, platelet-derived growth factor subunit A (PDGFA), fibroblast growth factor 2 (FGF2), and transforming growth factor beta 1 (TGF-β1) expression significantly increased from 6 to 24 h, 12 to 48 h, and 24 to 48 h, respectively. Imatinib, a specific inhibitor of platelet-derived growth factor receptor, significantly inhibited the proliferation of dermal fibroblasts promoted by PES, suggesting that PDGFA expression, an early response of PES, was involved in promoting the cell proliferation. Therefore, PES at 4800 Hz may initially promote PDGFA expression and subsequently stimulate the expression of two other growth factors, resulting in dermal fibroblast proliferation after 24 h or later. In conclusion, PES may activate the cell growth phase of wound healing.
MeSH term(s)	Adult ; Aged ; Cell Proliferation ; Dermis/metabolism ; Electric Stimulation ; Fibroblast Growth Factor 2/metabolism ; Fibroblasts/metabolism ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Imatinib Mesylate/pharmacology ; Intercellular Signaling Peptides and Proteins/metabolism ; Platelet-Derived Growth Factor/metabolism ; Time Factors ; Transforming Growth Factor beta1/metabolism ; Wound Healing
Chemical Substances	Intercellular Signaling Peptides and Proteins ; Platelet-Derived Growth Factor ; TGFB1 protein, human ; Transforming Growth Factor beta1 ; platelet-derived growth factor A ; Fibroblast Growth Factor 2 (103107-01-3) ; Imatinib Mesylate (8A1O1M485B)
Language	English
Publishing date	2020-09-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	184833-1
ISSN	1573-4919 ; 0300-8177
ISSN (online)	1573-4919
ISSN	0300-8177
DOI	10.1007/s11010-020-03912-6
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Zs.A 892: Show issues

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Article ; Online: Vertically integrated waveguide self-coupled resonator based tunable optical filter.

Zhai, Shan / Feng, Jijun / Sun, Xiaoyu / Akimoto, Ryoichi / Zeng, Heping

Optics letters

2018 Volume 43, Issue 15, Page(s) 3766–3769

Abstract: A vertically integrated waveguide self-coupled resonator based tunable optical filter was demonstrated. Unlike the conventional U-bend self-coupled waveguide structure, a top-layer S-bend waveguide was cross-coupled with the racetrack resonator on a ... ...

Abstract	A vertically integrated waveguide self-coupled resonator based tunable optical filter was demonstrated. Unlike the conventional U-bend self-coupled waveguide structure, a top-layer S-bend waveguide was cross-coupled with the racetrack resonator on a bottom layer. The different waveguide coupling effect was compared with the same resonance structure, which can realize the same free spectral range as well as a high quality factor. Spectrum response can be designed separately by varying the coupling coefficient between waveguide and resonator. A heater attached on the top of the resonator can be utilized for the resonance wavelength tuning, while a heater on the top of cross-coupled waveguide has little influence on the device performance, which can help to improve the stability. The presented device can also be applied as a tunable modulator/switch.
Language	English
Publishing date	2018-08-01
Publishing country	United States
Document type	Journal Article
ISSN	1539-4794
ISSN (online)	1539-4794
DOI	10.1364/OL.43.003766
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Article: Corrigendum: A high-flow nasal cannula system set at relatively low flow effectively washes out CO

Onodera, Yu / Akimoto, Ryo / Suzuki, Hiroto / Nakane, Masaki / Kawamae, Kaneyuki

Korean journal of anesthesiology

2018 Volume 71, Issue 1, Page(s) 75

Abstract: This corrects the article on p. 105 in vol. 70, PMID: 28184277.]. ...

Abstract	[This corrects the article on p. 105 in vol. 70, PMID: 28184277.].
Language	English
Publishing date	2018-02-01
Publishing country	Korea (South)
Document type	Journal Article ; Published Erratum
ZDB-ID	2557340-8
ISSN	2005-7563 ; 2005-6419
ISSN (online)	2005-7563
ISSN	2005-6419
DOI	10.4097/kjae.2018.71.1.75
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Article ; Online: Effect of belt electrode-skeletal muscle electrical stimulation on immobilization-induced muscle fibrosis.

Honda, Yuichiro / Tanaka, Natsumi / Kajiwara, Yasuhiro / Kondo, Yasutaka / Kataoka, Hideki / Sakamoto, Junya / Akimoto, Ryuji / Nawata, Atsushi / Okita, Minoru

PloS one

2021 Volume 16, Issue 5, Page(s) e0244120

Abstract: Purpose: Macrophage accumulation in response to decreasing myonuclei may be the major mechanism underlying immobilization-induced muscle fibrosis in muscle contracture, an intervention strategy suppressing these lesions is necessary. Therefore, this ... ...

Abstract	Purpose: Macrophage accumulation in response to decreasing myonuclei may be the major mechanism underlying immobilization-induced muscle fibrosis in muscle contracture, an intervention strategy suppressing these lesions is necessary. Therefore, this research investigated the effect of belt electrode-skeletal muscle electrical stimulation (B-SES), a new electrical stimulation device, to the macrophage accumulation via myonuclei decrease in immobilization-induced muscle fibrosis. Materials and methods: 18 Wistar male rats were divided into the control group, immobilization group (with plaster cast fixation to immobilize the soleus muscles in a shortened position for 2 weeks), and B-SES group (with muscle contractile exercise through B-SES during the immobilization period). B-SES stimulation was performed at a frequency of 50 Hz and an intensity of 4.7 mA, muscle contractile exercise by B-SES was applied to the lower limb muscles for 20 minutes/session (twice a day) for 2 weeks (6 times/week). The bilateral soleus muscles were used for histological, immunohistochemical, biochemical, and molecular biological analyses. Results: The number of myonuclei was significantly higher in the B-SES group than in the immobilization group, and there was no significant difference between the B-SES and control groups. The cross-sectional area of type I and II myofibers in the immobilization and B-SES groups was significantly lower than that in the control group, and the cross-sectional area of type I myofibers in the B-SES group was higher than that in the immobilization group. However, Atrogin-1 and MuRF-1 mRNA expression in the immobilization and B-SES groups was significantly higher than those in the control group. Additionally, the number of macrophages, IL-1β, TGF-β1, and α-SMA mRNA expression, and hydroxyproline expression was significantly lower in the control and B-SES groups than those in the immobilization group. Conclusion: This research surmised that muscle contractile exercise through B-SES prevented immobilization-induced muscle fibrosis, and this alteration suppressed the development of muscle contracture.
MeSH term(s)	Actins/genetics ; Actins/metabolism ; Animals ; Ankle/physiopathology ; Chemokine CCL2/genetics ; Chemokine CCL2/metabolism ; Electric Stimulation ; Electrodes ; Fibrosis ; Hydroxyproline/metabolism ; Immobilization ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; Joints/physiopathology ; Macrophages/pathology ; Male ; Muscle Fibers, Skeletal/pathology ; Muscle, Skeletal/pathology ; Muscle, Skeletal/physiopathology ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Range of Motion, Articular ; Rats, Wistar ; Transforming Growth Factor beta1/genetics ; Transforming Growth Factor beta1/metabolism ; Rats
Chemical Substances	Acta2 protein, rat ; Actins ; Ccl2 protein, rat ; Chemokine CCL2 ; Interleukin-1beta ; RNA, Messenger ; Transforming Growth Factor beta1 ; Hydroxyproline (RMB44WO89X)
Language	English
Publishing date	2021-05-13
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2267670-3
ISSN	1932-6203 ; 1932-6203
ISSN (online)	1932-6203
ISSN	1932-6203
DOI	10.1371/journal.pone.0244120
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