Article ; Online: Illuminating T cell-dendritic cell interactions in vivo by FlAsHing antigens.
2024 Volume 12
Abstract: Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for ... ...
| Abstract | Delineating the complex network of interactions between antigen-specific T cells and antigen presenting cells (APCs) is crucial for effective precision therapies against cancer, chronic infections, and autoimmunity. However, the existing arsenal for examining antigen-specific T cell interactions is restricted to a select few antigen-T cell receptor pairs, with limited in situ utility. This lack of versatility is largely due to the disruptive effects of reagents on the immune synapse, which hinder real-time monitoring of antigen-specific interactions. To address this limitation, we have developed a novel and versatile immune monitoring strategy by adding a short cysteine-rich tag to antigenic peptides that emits fluorescence upon binding to thiol-reactive biarsenical hairpin compounds. Our findings demonstrate the specificity and durability of the novel antigen-targeting probes during dynamic immune monitoring in vitro and in vivo. This strategy opens new avenues for biological validation of T-cell receptors with newly identified epitopes by revealing the behavior of previously unrecognized antigen-receptor pairs, expanding our understanding of T cell responses. |
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| MeSH term(s) | Epitopes ; Antigen-Presenting Cells ; Autoimmunity ; Cell Communication ; Cysteine |
| Chemical Substances | Epitopes ; Cysteine (K848JZ4886) |
| Language | English |
| Publishing date | 2024-01-18 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 2687154-3 |
| ISSN | 2050-084X ; 2050-084X |
| ISSN (online) | 2050-084X |
| ISSN | 2050-084X |
| DOI | 10.7554/eLife.91809 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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