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  1. Article ; Online: RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment Reveals Upregulation in Plant Stress and Defense Pathways

    Alexandra Waskow / Anthony Guihur / Alan Howling / Ivo Furno

    International Journal of Molecular Sciences, Vol 23, Iss 3070, p

    2022  Volume 3070

    Abstract: Not all agricultural practices are sustainable; however, non-thermal plasma treatment of seeds may be an eco-friendly alternative to improve macroscopic plant growth parameters. Despite the numerous successful results of plasma-seed treatments reported ... ...

    Abstract Not all agricultural practices are sustainable; however, non-thermal plasma treatment of seeds may be an eco-friendly alternative to improve macroscopic plant growth parameters. Despite the numerous successful results of plasma-seed treatments reported in the literature, there is a large gap in our understanding of how non-thermal plasma treatments affect seeds, especially due to the plethora of physical, chemical, and biological variables. This study uses RNA sequencing to characterize the changes in gene transcription in Arabidopsis thaliana (L.) Heynh. seeds 6 days after exposure to surface dielectric barrier discharge plasma treatment. Here, we provide an overview of all pathways that are differentially expressed where few genes are upregulated and many genes are downregulated. Our results reveal that plasma treatment time is a parameter that can activate different pathways in plant defense. An 80 s treatment upregulates the glucosinolate pathway, a defense response to insects and herbivores to deter feeding, whereas a shorter treatment of 60 s upregulates the phenylpropanoid pathway, which reinforces the cell wall with lignin and produces antimicrobial compounds, a defense response to bacterial or fungal plant pathogens. It seems that plasma elicits a wounding response from the seed in addition to redox changes. This suggests that plasma treatment can be potentially applied in agriculture to protect plants against abiotic and biotic stresses without discharging residues into the environment.
    Keywords non-thermal plasma ; RNA sequencing ; Arabidopsis thaliana ; oxidation-reduction ; plant defense ; secondary metabolism ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2022-03-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  2. Article ; Online: Catabolism of Glucosinolates into Nitriles Revealed by RNA Sequencing of Arabidopsis thaliana Seedlings after Non-Thermal Plasma-Seed Treatment

    Alexandra Waskow / Anthony Guihur / Alan Howling / Ivo Furno

    Life, Vol 12, Iss 1822, p

    2022  Volume 1822

    Abstract: Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis ... ...

    Abstract Non-thermal plasma-seed treatments could be an environmentally friendly method to modulate plant properties. Since it remains unclear how plasmas affect seeds, RNA sequencing was used here to analyze gene transcription changes in 7-day-old Arabidopsis thaliana (L.) Heynh. seedlings grown from surface dielectric barrier discharge plasma-treated seeds. In a previous study, seeds were analyzed 6 days after plasma exposure and a plant stress and defense response was observed. Here, we performed a pathway analysis on differentially expressed genes and our results revealed again an increased expression of plant stress and defense, specifically glucosinolate pathway-related compounds. The main difference was that a different part of the plant defense response changed at 7 days, which was not previously observed at 6 days. With a 24-h delayed extraction time point, the glucosinolates were selectively broken down into nitriles among all of the glucosinolates catabolic products. Although information about nitriles is limited, it protects plants against biotic stresses and has variable toxicity depending on the interacting organism. More work needs to be performed to better understand which plasma seed treatment parameters affect plant defense; however, these preliminary findings suggest that an optimized plasma treatment could be used to elicit a plant defense response.
    Keywords non-thermal plasma ; plant defense ; glucosinolates ; nitriles ; RNA sequencing ; Arabidopsis thaliana ; Science ; Q
    Subject code 580
    Language English
    Publishing date 2022-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: An In Situ FTIR Study of DBD Plasma Parameters for Accelerated Germination of Arabidopsis thaliana Seeds

    Alexandra Waskow / Lorenzo Ibba / Max Leftley / Alan Howling / Paolo F. Ambrico / Ivo Furno

    International Journal of Molecular Sciences, Vol 22, Iss 11540, p

    2021  Volume 11540

    Abstract: Current agricultural practices are not sustainable; however, the non-thermal plasma treatment of seeds may be an eco-friendly alternative to alter macroscopic plant growth parameters. Despite numerous successful results of plasma-seed treatments reported ...

    Abstract Current agricultural practices are not sustainable; however, the non-thermal plasma treatment of seeds may be an eco-friendly alternative to alter macroscopic plant growth parameters. Despite numerous successful results of plasma-seed treatments reported in the literature, the plasma-treatment parameters required to improve plant growth remain elusive due to the plethora of physical, chemical, and biological variables. In this study, we investigate the optimal conditions in our surface dielectric barrier discharge (SDBD) setup, using a parametric study, and attempt to understand relevant species in the plasma treatment using in situ Fourier transform infrared (FTIR) absorption spectroscopy. Our results suggest that treatment time and voltage are key parameters for accelerated germination; however, no clear conclusion on causative agents can be drawn.
    Keywords plasma ; Arabidopsis thaliana ; FTIR ; germination ; reactive oxygen and nitrogen species ; surface DBD ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 580
    Language English
    Publishing date 2021-10-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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