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  1. Article: Royal Jelly and Aliskiren mutually annul their protective effects against gentamicin-induced nephrotoxicity in rats.

    Alaraj, Mohd

    Veterinary world

    2020  Volume 13, Issue 12, Page(s) 2658–2662

    Abstract: Background and aim: Gentamicin (GM) is one of the most effective antibiotics for severe, life-threatening Gram-negative infections. Nevertheless, its clinical use has been restrained because of its nephrotoxic potential. Royal jelly (RJ) and aliskiren ( ... ...

    Abstract Background and aim: Gentamicin (GM) is one of the most effective antibiotics for severe, life-threatening Gram-negative infections. Nevertheless, its clinical use has been restrained because of its nephrotoxic potential. Royal jelly (RJ) and aliskiren (ALK) can individually prevent such toxic effects. The aim of this study was to explore the protective effects of a combination treatment of RJ and ALK on GM-mediated nephrotoxicity.
    Materials and methods: Thirty-two adult female. Wistar rats were divided equally into four groups: (I) Receiving normal saline; (II) GM (100 mg/kg, intraperitoneal [i.p.] injection); GM (100 mg/kg, i.p. injection) plus ALK (50 mg/kg, i.p. injection); and (IV) GM (100 mg/kg, i.p. injection) plus ALK (50 mg/kg, i.p. injection) in combination with RJ (150 mg/kg, orally). All treatments were administered daily for 10 days. The blood levels of creatinine, urea, uric acid, albumin, and total protein were measured. Then, the animals were sacrificed, and the kidneys were taken for histopathology.
    Results: Compared to normal control rats, GM-injected rats showed significantly (p<0.001) higher serum concentrations of uric acid, urea, and creatinine as well as evidently (p<0.001) lower blood levels of albumin and total protein. Moreover, GM administration was associated with significant renal histopathological changes. All these alterations were considerably (p<0.05) improved in GM-injected rats receiving ALK compared to rats receiving GM alone. However, when RJ was given in combination with ALK to GM-injected rats, it lessened the beneficial nephroprotective effects of both agents.
    Conclusion: The combination treatment of RJ and ALK is not desirable for GM-induced nephrotoxicity. Further studies are crucial to accurately explore the precise mechanism of RJ antagonistic interaction with ALK.
    Language English
    Publishing date 2020-12-14
    Publishing country India
    Document type Journal Article
    ZDB-ID 2456277-4
    ISSN 2231-0916 ; 0972-8988
    ISSN (online) 2231-0916
    ISSN 0972-8988
    DOI 10.14202/vetworld.2020.2658-2662
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: A comprehensive computational study to explore promising natural bioactive compounds targeting glycosyltransferase MurG in Escherichia coli for potential drug development.

    Shtaiwi, Amneh / Khan, Shafi Ullah / Khedraoui, Meriem / Alaraj, Mohd / Samadi, Abdelouahid / Chtita, Samir

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 7098

    Abstract: Peptidoglycan is a carbohydrate with a cross-linked structure that protects the cytoplasmic membrane of bacterial cells from damage. The mechanism of peptidoglycan biosynthesis involves the main synthesizing enzyme glycosyltransferase MurG, which is ... ...

    Abstract Peptidoglycan is a carbohydrate with a cross-linked structure that protects the cytoplasmic membrane of bacterial cells from damage. The mechanism of peptidoglycan biosynthesis involves the main synthesizing enzyme glycosyltransferase MurG, which is known as a potential target for antibiotic therapy. Many MurG inhibitors have been recognized as MurG targets, but high toxicity and drug-resistant Escherichia coli strains remain the most important problems for further development. In addition, the discovery of selective MurG inhibitors has been limited to the synthesis of peptidoglycan-mimicking compounds. The present study employed drug discovery, such as virtual screening using molecular docking, drug likeness ADMET proprieties predictions, and molecular dynamics (MD) simulation, to identify potential natural products (NPs) for Escherichia coli. We conducted a screening of 30,926 NPs from the NPASS database. Subsequently, 20 of these compounds successfully passed the potency, pharmacokinetic, ADMET screening assays, and their validation was further confirmed through molecular docking. The best three hits and the standard were chosen for further MD simulations up to 400 ns and energy calculations to investigate the stability of the NPs-MurG complexes. The analyses of MD simulations and total binding energies suggested the higher stability of NPC272174. The potential compounds can be further explored in vivo and in vitro for promising novel antibacterial drug discovery.
    MeSH term(s) Glycosyltransferases/metabolism ; Escherichia coli/metabolism ; Bacterial Outer Membrane Proteins/metabolism ; Molecular Docking Simulation ; Peptidoglycan ; Anti-Bacterial Agents/pharmacology ; Molecular Dynamics Simulation ; Drug Development
    Chemical Substances Glycosyltransferases (EC 2.4.-) ; Bacterial Outer Membrane Proteins ; Peptidoglycan ; Anti-Bacterial Agents
    Language English
    Publishing date 2024-03-26
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-57702-x
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Advancing therapeutic efficacy: nanovesicular delivery systems for medicinal plant-based therapeutics.

    Saadh, Mohamed J / Mustafa, Mohammed Ahmed / Kumar, Sanjay / Gupta, Pooja / Pramanik, Atreyi / Rizaev, Jasur Alimdjanovich / Shareef, Hasanain Khaleel / Alubiady, Mahmood Hasen Shuhata / Al-Abdeen, Salah Hassan Zain / Shakier, Hussein Ghafel / Alaraj, Mohd / Alzubaidi, Laith H

    Naunyn-Schmiedeberg's archives of pharmacology

    2024  

    Abstract: The utilization of medicinal plant extracts in therapeutics has been hindered by various challenges, including poor bioavailability and stability issues. Nanovesicular delivery systems have emerged as promising tools to overcome these limitations by ... ...

    Abstract The utilization of medicinal plant extracts in therapeutics has been hindered by various challenges, including poor bioavailability and stability issues. Nanovesicular delivery systems have emerged as promising tools to overcome these limitations by enhancing the solubility, bioavailability, and targeted delivery of bioactive compounds from medicinal plants. This review explores the applications of nanovesicular delivery systems in antibacterial and anticancer therapeutics using medicinal plant extracts. We provide an overview of the bioactive compounds present in medicinal plants and their therapeutic properties, emphasizing the challenges associated with their utilization. Various types of nanovesicular delivery systems, including liposomes, niosomes, ethosomes, and solid lipid nanoparticles, among others, are discussed in detail, along with their potential applications in combating bacterial infections and cancer. The review highlights specific examples of antibacterial and anticancer activities demonstrated by these delivery systems against a range of pathogens and cancer types. Furthermore, we address the challenges and limitations associated with the scale-up, stability, toxicity, and regulatory considerations of nanovesicular delivery systems. Finally, future perspectives are outlined, focusing on emerging technologies, integration with personalized medicine, and potential collaborations to drive forward research in this field. Overall, this review underscores the potential of nanovesicular delivery systems for enhancing the therapeutic efficacy of medicinal plant extracts in antibacterial and anticancer applications, while identifying avenues for further research and development.
    Language English
    Publishing date 2024-05-03
    Publishing country Germany
    Document type Journal Article ; Review
    ZDB-ID 121471-8
    ISSN 1432-1912 ; 0028-1298
    ISSN (online) 1432-1912
    ISSN 0028-1298
    DOI 10.1007/s00210-024-03104-9
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    In stock of ZB MED Cologne/Königswinter

    Ud II Zs.5: Show issues Location:
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  4. Article: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats.

    Al-Trad, Bahaa / Ashankyty, Ibraheem M / Alaraj, Mohd

    Diabetology & metabolic syndrome

    2015  Volume 7, Page(s) 97

    Abstract: Background: Previous studies reported that 17β-estradiol may influence the progression of diabetic renal disease in females. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in ...

    Abstract Background: Previous studies reported that 17β-estradiol may influence the progression of diabetic renal disease in females. The present study was intended to provide an insight into the specific effects of progesterone, the other female sex hormone, in the diabetic renal complications.
    Methods: Adult female wistar rats were divided into four groups (n = 6/group): intact control (non-diabetic, ND), intact diabetic (D), ovariectomized diabetic (D-OVX) and ovariectomized diabetic which were treated with progesterone (D-OVX + P; 10 mg/kg, s.c, every second day) for 10 weeks. Diabetes was induced by a single dose injection of 55 mg/kg streptozotocin. Expressions of transforming growth factor-β (TGF-β), fibronectin, vascular endothelial growth factor-A (VEGF-A), angiotensin II type 1 receptor (ATR1) and podocyte markers (nephrin and podocin) were assessed by immunohistochemistry and real-time PCR.
    Results: The treatment of D-OVX rats with progesterone attenuated diabetic-associated increases in the urinary albumin to creatinine ratio, glomerulosclerosi and the expression of profibrotic and angiogenic factors (TGF-β, Fibronectin and VEGF-A). Furthermore, progesterone supplementation prevented diabetes-induced downregulation of nephrin and podocin while the overexpression of ATR1 in the diabetic rats was inhibited by the progesterone supplementation.
    Conclusion: These results provided evidence, for the first time, that the replacement of progesterone can ameliorate the renal damage in the experimental models of diabetic nephropathy through improving the renal function; the inhibition of renal fibrosis and abnormal angiogenesis; along with the amelioration of podocyte injury. Additionally, the blocking of renin-angiotensin system through the down-regulation of ATR1 expression may also account for the reno-protective effect of progesterone.
    Language English
    Publishing date 2015-11-14
    Publishing country England
    Document type Journal Article
    ZDB-ID 2518786-7
    ISSN 1758-5996
    ISSN 1758-5996
    DOI 10.1186/s13098-015-0097-1
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  5. Article: Pretreatment with

    Alaraj, Mohd / Acar, Tolgahan / Kosinska, Irena / Al-Trad, Bahaa / Almaaytah, Ammar M / Saadh, Mohamed J / Qumani, Mohammed A / Syed, Shahid M / Altaif, Khalil I / Ashfaque, Hossain

    Veterinary world

    2021  Volume 14, Issue 3, Page(s) 589–594

    Abstract: Background and aim: Paracetamol (PCM) ingestion is one of the most frequent global causes of toxicity. : Materials and methods: Mice were given PCM with and without : Results: A single overdose of PCM caused significant elevations of alanine and ... ...

    Abstract Background and aim: Paracetamol (PCM) ingestion is one of the most frequent global causes of toxicity.
    Materials and methods: Mice were given PCM with and without
    Results: A single overdose of PCM caused significant elevations of alanine and aspartate transaminases, alkaline phosphate, bilirubin, urea, uric acid, and creatinine compared with the control group. In addition, PCM toxicity significantly lowered red blood cell count but insignificantly increased both white blood cell and platelet counts in comparison to the control mice. Pretreatment with
    Conclusion: Taken together,
    Language English
    Publishing date 2021-03-08
    Publishing country India
    Document type Journal Article
    ZDB-ID 2456277-4
    ISSN 2231-0916 ; 0972-8988
    ISSN (online) 2231-0916
    ISSN 0972-8988
    DOI 10.14202/vetworld.2021.589-594
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  6. Article: Hyperglycaemia and intramitochondrial glycogen granules in the brain of mice. Ultrastructural study.

    Alaraj, Mohd / Gajkowska, Barbara / Cholewiński, Marcin / Lazarewicz, Jerzy W

    Folia neuropathologica

    2004  Volume 42, Issue 2, Page(s) 113–118

    Abstract: The mechanism of cytotoxic effects of hyperglycaemia on the brain has not yet been explained and the proposed hypotheses are not fully convincing. In the present study, we aimed to assess the effect of high doses of glucose on the ultrastructure of the ... ...

    Abstract The mechanism of cytotoxic effects of hyperglycaemia on the brain has not yet been explained and the proposed hypotheses are not fully convincing. In the present study, we aimed to assess the effect of high doses of glucose on the ultrastructure of the mice brain. The results, which are in agreement with the literature data, show that the administration of a single high dose of glucose, as well as its chronic application, leads to accumulation of glycogen granules in the cytoplasm of astrocytes. A new observation is the detection of glycogen granules in the ultrastructurally changed mitochondria of astrocytes as well as in the mitochondria of some synapses. Our hypothesis assumes that excess of glucose may cause an increase in the vulnerability of the brain mitochondria. This in turn may enable glucose and cytoplasmic enzymes to penetrate into the mitochondria and they therein synthesise glycogen. Mitochondrial dysfunction may in turn lead to neurodegeneration by apoptotic process.
    MeSH term(s) Animals ; Brain/drug effects ; Brain/ultrastructure ; Glucose/pharmacology ; Glycogen/analysis ; Hyperglycemia/metabolism ; Hyperglycemia/pathology ; Male ; Mice ; Mitochondria/chemistry ; Mitochondria/drug effects ; Mitochondria/ultrastructure ; Submitochondrial Particles/chemistry ; Submitochondrial Particles/drug effects ; Submitochondrial Particles/ultrastructure
    Chemical Substances Glycogen (9005-79-2) ; Glucose (IY9XDZ35W2)
    Language English
    Publishing date 2004
    Publishing country Poland
    Document type Journal Article
    ZDB-ID 1310363-5
    ISSN 1509-572X ; 1641-4640 ; 0028-3894
    ISSN (online) 1509-572X
    ISSN 1641-4640 ; 0028-3894
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 716: Show issues Location:
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