LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 7 of total 7

Search options

  1. Article ; Online: Haberlea rhodopensis Extract Tunes the Cellular Response to Stress by Modulating DNA Damage, Redox Components, and Gene Expression

    Dessislava Staneva / Neli Dimitrova / Borislav Popov / Albena Alexandrova / Milena Georgieva / George Miloshev

    International Journal of Molecular Sciences, Vol 24, Iss 21, p

    2023  Volume 15964

    Abstract: Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise ... ...

    Abstract Ionizing radiation (IR) and reactive oxygen species (ROS)-induced oxidative stress can cause damage to cellular biomolecules, including DNA, proteins, and lipids. These harmful effects can compromise essential cellular functions and significantly raise the risk of metabolic dysfunction, accumulation of harmful mutations, genome instability, cancer, accelerated cellular senescence, and even death. Here, we present an investigation of HeLa cancer cells’ early response to gamma IR (γ-IR) and oxidative stress after preincubation of the cells with natural extracts of the resurrection plant Haberlea rhodopensis . In light of the superior protection offered by plant extracts against radiation and oxidative stress, we investigated the cellular defence mechanisms involved in such protection. Specifically, we sought to evaluate the molecular effects of H. rhodopensis extract (HRE) on cells subjected to genotoxic stress by examining the components of the redox pathway and quantifying the transcription levels of several critical genes associated with DNA repair, cell cycle regulation, and apoptosis. The influence of HRE on genome integrity and the cell cycle was also studied via comet assay and flow cytometry. Our findings demonstrate that HREs can effectively modulate the cellular response to genotoxic and oxidative stress within the first two hours following exposure, thereby reducing the severity of such stress. Furthermore, we observed the specificity of genoprotective HRE doses depending on the source of the applied genotoxic stress.
    Keywords Haberlea rhodopensis ; gamma irradiation ; oxidative stress ; genotoxicity ; antioxidant ; gene transcription ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 500
    Language English
    Publishing date 2023-11-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Chromatographic Profile and Redox-Modulating Capacity of Methanol Extract from Seeds of Ginkgo biloba L. Originating from Plovdiv Region in Bulgaria

    Lubomir Petrov / Albena Alexandrova / Mariana Argirova / Teodora Tomova / Almira Georgieva / Elina Tsvetanova / Milka Mileva

    Life, Vol 12, Iss 878, p

    2022  Volume 878

    Abstract: Oxidative stress underlies the pathogenesis of many diseases, which determines the interest in natural substances with antioxidant properties. Ginkgo biloba L. leaves are well known and widely used in the pharmaceutical industry, but the therapeutic ... ...

    Abstract Oxidative stress underlies the pathogenesis of many diseases, which determines the interest in natural substances with antioxidant properties. Ginkgo biloba L. leaves are well known and widely used in the pharmaceutical industry, but the therapeutic properties of the seeds are less studied. This study aimed to identify the chromatographic profile and to evaluate the antioxidant properties of methanol extract from seeds of G. biloba (GBSE). In the GBSE, flavonoids and terpenes were found as terpenes predominated. The GBSE antioxidant capacity determined by 2,2 azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) and 1-diphenyl-2-picrylhydrazyl (DPPH) methods were equal to 1.34% and 0.58% of the activity of reference substance Trolox, respectively. The results of the ferric reducing antioxidant power method showed that the effect of concentration 1 mg/mL ( w / v ) GBSE was equal to 7.418 mM FeSO 4 used as a standard. The cupric reducing antioxidant capacity activity of the GBSE was found to be 215.39 µmol Trolox/g GBSE and is presented as Trolox equivalent. The metal chelation effect of 1 mg/mL ( w / v ) GBSE was equal to that obtained for 0.018 mM EDTA. In conclusion, GBSE showed a good ability to neutralize ABTS and DPPH radicals and could have a beneficial effect in pathological conditions with oxidative stress etiology.
    Keywords Ginkgo biloba seeds extract ; phytochemical profile ; antiradical activity ; metal-reducing and metal chelating effect ; Science ; Q
    Language English
    Publishing date 2022-06-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article ; Online: Role of Trace Elements for Oxidative Status and Quality of Human Sperm

    Galina Nenkova / Lubomir Petrov / Albena Alexandrova

    Balkan Medical Journal, Vol 34, Iss 4, Pp 343-

    2017  Volume 348

    Abstract: Background: Oxidative stress affects sperm quality negatively. To maintain the pro/antioxidant balance, some metal ions (e.g. copper, zink, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could ... ...

    Abstract Background: Oxidative stress affects sperm quality negatively. To maintain the pro/antioxidant balance, some metal ions (e.g. copper, zink, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could act as prooxidants inducing oxidative damage of spermatozoa. Aims: To reveal a possible correlation between the concentrations of some metal ions (iron, copper, zinc, and selenium) in human seminal plasma, oxidative stress, assessed by malondialdehyde and total glutathione levels, and semen quality, assessed by the parameters count, motility, and morphology. Study Design: Descriptive study. Methods: The semen analysis for volume, count, and motility was performed according to World Health Organization (2010) guidelines, using computer-assisted semen analysis. For the determination of spermatozoa morphology, a SpermBlue staining method was applied. Depending on their parameters, the sperm samples were categorized into normozoospermic, teratozoospermic, asthenoteratozoospermic, and oligoteratozoospermic. The seminal plasma content of iron, copper, zinc, and selenium was estimated by atomic absorption spectroscopy. The malondialdehyde and total glutathione levels were quantified spectrophotometrically. Results: In the groups with poor sperm quality, the levels of Fe were higher, whereas those of Zn and Se were significantly lower than in the normozoospermic group. In all groups with poor sperm quality, increased levels of malondialdehyde and decreased glutathione levels were detected as evidence of oxidative stress occurrence. All these differences are most pronounced in the asthenoteratozoospermic group where values differ nearly twice as much compared to the normozoospermic group. The Fe concentration correlated positively with the malondialdehyde (r=0.666, p=0.018), whereas it showed a negative correlation with the level of total glutathione (r=-0.689, p=0.013). The total glutathione level correlated positively with the sperm motility (r=0.589, p=0.044). ...
    Keywords Metal ions ; oxidative status ; sperm ; Medicine ; R
    Subject code 630
    Language English
    Publishing date 2017-08-01T00:00:00Z
    Publisher Galenos Publishing House
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: A review

    Galina Nenkova / Albena Alexandrova

    Advances in Bioscience and Biotechnology, Vol 04, Iss 01, Pp 37-

    Oxidative stress and its role in reproduction

    2013  Volume 43

    Abstract: Recently the problem of infertility has become more acute. About 3% - 7% of couples suffer a lasting infertility problem and there is a tendency of constant increasing of this number. Existing data reveal that in many of the infertility cases, the cause ... ...

    Abstract Recently the problem of infertility has become more acute. About 3% - 7% of couples suffer a lasting infertility problem and there is a tendency of constant increasing of this number. Existing data reveal that in many of the infertility cases, the cause of sterility is the disabled sperm as a result of the oxidative stress (OS). There are several sources of reactive oxygen species (ROS) in the sperm. Both enzymatic and nonenzymatic antioxidants act as a counteraction against ROS production in seminal plasma. However due to the limited cytoplasm, the spermatozoa are more susceptible to oxidative damage because of the lack of protective cytoplasmic antioxidant enzymes. Currently, many couples suffering fertility problems resort to assisted reproductive technology (ART). Unfortunately, the success rate of ART is relatively low—about 30%. Sperm processing incl. centrifugation, freezing and thawing is associated with the generation of ROS. A range of strategies for sperm prevention of OS are developed. Many studies concern antioxidants and their impact on sperm function, but there are a lot of contradictions in terms of doses and types of combinations that can be used in ART. Future studies should aim the creation of standard and reliable operating procedures for increasing the successfulness of these techniques. This paper offers an overview of the impact of OS on the reproduction, pointing out the sources of ROS in the sperm, the antioxidant defense systems, and the strategies for prevention and improvement of the sperm reproductive functions, incl. those applied in ART.
    Keywords Antioxidants ; Assisted Reproductive Technology ; Reactive Oxygen Species ; Sperm ; Biology (General) ; QH301-705.5 ; Science ; Q ; DOAJ:Biology ; DOAJ:Biology and Life Sciences
    Subject code 501
    Language English
    Publishing date 2013-01-01T00:00:00Z
    Publisher Scientific Research Publishing
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  5. Article ; Online: New Myrtenal–Adamantane Conjugates Alleviate Alzheimer’s-Type Dementia in Rat Model

    Stela Dragomanova / Maria Lazarova / Aldar Munkuev / Evgeniy Suslov / Konstantin Volcho / Nariman Salakhutdinov / Amina Bibi / Jóhannes Reynisson / Elina Tzvetanova / Albena Alexandrova / Almira Georgieva / Diamara Uzunova / Miroslava Stefanova / Reni Kalfin / Lyubka Tancheva

    Molecules, Vol 27, Iss 5456, p

    2022  Volume 5456

    Abstract: Alzheimer’s disease (AD) is a neurodegenerative disease associated with memory impairment and other central nervous system (CNS) symptoms. Two myrtenal–adamantane conjugates (MACs) showed excellent CNS potential against Alzheimer’s models. Adamantane is ... ...

    Abstract Alzheimer’s disease (AD) is a neurodegenerative disease associated with memory impairment and other central nervous system (CNS) symptoms. Two myrtenal–adamantane conjugates (MACs) showed excellent CNS potential against Alzheimer’s models. Adamantane is a common pharmacophore for drug design, and myrtenal (M) demonstrated neuroprotective effects in our previous studies. The aim of this study is to evaluate the MACs’ neuroprotective properties in dementia. Methods: Scopolamine (Scop) was applied intraperitoneally in Wistar rats for 11 days, simultaneously with MACs or M as a referent, respectively. Brain acetylcholine esterase (AChE) activity, noradrenaline and serotonin levels, and oxidative brain status determination followed behavioral tests on memory abilities. Molecular descriptors and docking analyses for AChE activity center affinity were performed. Results: M derivatives have favorable physicochemical parameters to enter the CNS. Both MACs restored memory damaged by Scop, showing significant AChE-inhibitory activity in the cortex, in contrast to M, supported by the modeling analysis. Moderate antioxidant properties were manifested by glutathione elevation and catalase activity modulation. MACs also altered noradrenaline and serotonin content in the hippocampus. Conclusion: For the first time, neuroprotective properties of two MACs in a rat dementia model were observed. They were stronger than the natural M effects, which makes the substances promising candidates for AD treatment.
    Keywords neuroprotection ; experimental dementia ; myrtenal–adamantane conjugates ; acetylcholinesterase ; monoamines ; memory ; Organic chemistry ; QD241-441
    Subject code 540
    Language English
    Publishing date 2022-08-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  6. Article ; Online: Effects of anthropogenic and environmental stressors on the current status of red mullet (Mullus barbatus L., 1758) populations inhabiting the Bulgarian Black Sea waters

    Ivelina Zlateva / Violin Raykov / Albena Alexandrova / Petya Ivanova / Nesho Chipev / Kremena Stefanova / Nina Dzhembekova / Valentina Doncheva / Violeta Slabakova / Elitsa Stefanova / Svetlana Mihova / Nadezhda Valcheva / Ognyana Hristova / Boryana Dzhurova / Dimitar Dimitrov / Almira Georgieva / Elina Tsvetanova / Madlena Andreeva / Ivan Popov /
    Mariya Yankova / Yordan Raev / Konstantin Petrov

    Nature Conservation, Vol 54, Iss , Pp 55-

    2023  Volume 79

    Abstract: The red mullet (Mullus barbatus Linnaeus, 1758) is a keynote species for the Bulgarian Black Sea ecosystem and fisheries; nevertheless, existing knowledge on population status is very scarce. The present study was intended to assess the health status and ...

    Abstract The red mullet (Mullus barbatus Linnaeus, 1758) is a keynote species for the Bulgarian Black Sea ecosystem and fisheries; nevertheless, existing knowledge on population status is very scarce. The present study was intended to assess the health status and adaptive potential of M. barbatus populations inhabiting the Bulgarian waters of the Black Sea. Our findings revealed that populations of M. barbatus are exposed to a variety of anthropogenic and environmental stressors. The species’ status was assessed using representative genetic, morphological, biochemical and chemical biomarkers from specimens obtained in the research area’s northern and southern regions. Based on mtDNA markers, genetic analysis revealed low haplotype and nucleotide diversity, typically observed in overexploited or “threatened” populations. Examining the morphology of the specimens revealed no discernible pattern of differentiation. Except for aluminium and chrome, metal and PAH concentrations in fish were below the regulatory thresholds. The specimens from the southern region ingested more microplastics than those from the northern region. The majority of specimens collected from the southern region also exhibited elevated levels of oxidative stress and decreased antioxidant defence, which can be interpreted as an early indication that they had reached the limits of their adaptive potential. Further research on the composite effects of the stressogenic environment on the Black Sea biota are critically needed, as well as the introduction of new indicators and thresholds at molecular and cellular levels for adequate monitoring of both the ecological state of the marine environment and its biota.
    Keywords Ecology ; QH540-549.5 ; General. Including nature conservation ; geographical distribution ; QH1-199.5
    Subject code 333
    Language English
    Publishing date 2023-12-01T00:00:00Z
    Publisher Pensoft Publishers
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  7. Article: Samardala: specificities and changes in the ethnobotanical knowledge about Allium siculum subsp. dioscoridis (Sm.) K. Richt. in Bulgaria

    Ivanova, Teodora / Albena Alexandrova / Almira Georgieva / Dessislava Dimitrova / Elina Tsvetanova / Mihail Chervenkov / Stoyan Chervenkov / Tatyana Stoeva / Yulia Bosseva

    Genetic resources and crop evolution. 2018 June, v. 65, no. 5

    2018  

    Abstract: Allium siculum Ucria is a perennial bulbous plant that occurs in Bulgaria as A. siculum subsp. dioscoridis (Sm.) K. Richt. The plant is locally known as “samardala”. Its herbage is used traditionally by local communities in Bulgaria as edible green and ... ...

    Abstract Allium siculum Ucria is a perennial bulbous plant that occurs in Bulgaria as A. siculum subsp. dioscoridis (Sm.) K. Richt. The plant is locally known as “samardala”. Its herbage is used traditionally by local communities in Bulgaria as edible green and for preparation of salty spice mixtures. The study was focused on the documentation of traditional knowledge about samardala, covering local collection and cultivation practices, processing methods and consumption preferences. The respondents (aged 30–86 years, 50% over 65) were local producers and small-scale salesmen of the herbage and samardala-containing spices. To assess the health features of samardala spices the total phenol and flavonoid content was measured in home-consumed or marketed flavoured salts. Overall appreciation of the plant and related food products was found to be very high; presenting a considerable engagement of the locals in plant cultivation and gathering, laborious processing and consumption. Traditional ecological knowledge about the biology, phenology and ecology of the samardala plant and methods for its processing is still preserved by elderly people who rely mostly on their own cultivated plants. Although the introduction of modern appliances has altered the method of production, its main stages and the recipes are preserved. Modernization of the processing method has not affected the quality of the products and faster processing could be a contributing factor for the higher content of biologically active substances. Phenolic content was about twice as low as the flavonoid content, following the same pattern for all tested flavoured salts. Measures for better management of natural populations and promotion of cultivation practices of A. siculum subsp. dioscoridis would ensure the sustainable quality of traditional products as well as conservation of the natural plant resources.
    Keywords active ingredients ; Allium ; elderly ; environmental knowledge ; ethnobotany ; flavonoids ; foods ; forage ; indigenous knowledge ; modernization ; phenology ; phenols ; processing technology ; recipes ; salts ; spices ; Bulgaria
    Language English
    Dates of publication 2018-06
    Size p. 1349-1357.
    Publishing place Springer Netherlands
    Document type Article
    ZDB-ID 1134125-7
    ISSN 0925-9864
    ISSN 0925-9864
    DOI 10.1007/s10722-018-0618-5
    Database NAL-Catalogue (AGRICOLA)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Bonn / Germany

    Z 4526: Show issues

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top