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Article ; Online: Highly Attenuated Poxvirus-Based Vaccines Against Emerging Viral Diseases

Perdiguero, Beatriz / Pérez, Patricia / Marcos-Villar, Laura / Albericio, Guillermo / Astorgano, David / Alvarez, Enrique / Sin, Laura / Gómez, Carmen Elena / García-Arriaza, Juan / Esteban de Vega, Mariano

Journal of Molecular Biology. 2023 Aug., v. 435, no. 15 p.168173-

2023

Abstract: Although one member of the poxvirus family, variola virus, has caused one of the most devastating human infections worldwide, smallpox, the knowledge gained over the last 30 years on the molecular, virological and immunological mechanisms of these ... ...

Abstract	Although one member of the poxvirus family, variola virus, has caused one of the most devastating human infections worldwide, smallpox, the knowledge gained over the last 30 years on the molecular, virological and immunological mechanisms of these viruses has allowed the use of members of this family as vectors for the generation of recombinant vaccines against numerous pathogens. In this review, we cover different aspects of the history and biology of poxviruses with emphasis on their application as vaccines, from first- to fourth-generation, against smallpox, monkeypox, emerging viral diseases highlighted by the World Health Organization (COVID-19, Crimean-Congo haemorrhagic fever, Ebola and Marburg virus diseases, Lassa fever, Middle East respiratory syndrome and severe acute respiratory syndrome, Nipah and other henipaviral diseases, Rift Valley fever and Zika), as well as against one of the most concerning prevalent virus, the Human Immunodeficiency Virus, the causative agent of Acquired Immunodeficiency Syndrome. We discuss the implications in human health of the 2022 monkeypox epidemic affecting many countries, and the rapid prophylactic and therapeutic measures adopted to control virus dissemination within the human population. We also describe the preclinical and clinical evaluation of the Modified Vaccinia virus Ankara and New York vaccinia virus poxviral strains expressing heterologous antigens from the viral diseases listed above. Finally, we report different approaches to improve the immunogenicity and efficacy of poxvirus-based vaccine candidates, such as deletion of immunomodulatory genes, insertion of host-range genes and enhanced transcription of foreign genes through modified viral promoters. Some future prospects are also highlighted.
Keywords	COVID-19 infection ; Crimean-Congo hemorrhagic fever ; Human immunodeficiency virus ; Lassa virus fever ; Marburg marburgvirus ; Rift Valley fever ; Vaccinia virus ; Variola virus ; World Health Organization ; acquired immunodeficiency syndrome ; clinical examination ; etiological agents ; host range ; human health ; human population ; humans ; immunogenicity ; molecular biology ; smallpox ; therapeutics ; vaccines ; viruses ; New York ; Poxviruses ; vaccinia virus-based MVA and NYVAC vectors ; smallpox and monkeypox ; emerging viruses
Language	English
Dates of publication	2023-08
Publishing place	Elsevier Ltd
Document type	Article ; Online
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2023.168173
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Comparative proteomic analysis of nuclear and cytoplasmic compartments in human cardiac progenitor cells.

Albericio, Guillermo / Aguilar, Susana / Torán, Jose Luis / Yañez, Rosa / López, Juan Antonio / Vázquez, Jesús / Mora, Carmen / Bernad, Antonio

Scientific reports

2022 Volume 12, Issue 1, Page(s) 146

Abstract: Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their therapeutic ... ...

Abstract	Clinical trials evaluating cardiac progenitor cells (CPC) demonstrated feasibility and safety, but no clear functional benefits. Therefore a deeper understanding of CPC biology is warranted to inform strategies capable to enhance their therapeutic potential. Here we have defined, using a label-free proteomic approach, the differential cytoplasmic and nuclear compartments of human CPC (hCPC). Global analysis of cytoplasmic repertoire in hCPC suggested an important hypoxia response capacity and active collagen metabolism. In addition, comparative analysis of the nuclear protein compartment identified a significant regulation of a small number of proteins in hCPC versus human mesenchymal stem cells (hMSC). Two proteins significantly upregulated in the hCPC nuclear compartment, IL1A and IMP3, showed also a parallel increase in mRNA expression in hCPC versus hMSC, and were studied further. IL1A, subjected to an important post-transcriptional regulation, was demonstrated to act as a dual-function cytokine with a plausible role in apoptosis regulation. The knockdown of the mRNA binding protein (IMP3) did not negatively impact hCPC viability, but reduced their proliferation and migration capacity. Analysis of a panel of putative candidate genes identified HMGA2 and PTPRF as IMP3 targets in hCPC. Therefore, they are potentially involved in hCPC proliferation/migration regulation.
MeSH term(s)	Cell Movement ; Cell Nucleus/metabolism ; Cell Proliferation ; Cells, Cultured ; Cytoplasm/metabolism ; Fibroblasts/metabolism ; Gene Expression Regulation ; HMGA2 Protein/genetics ; HMGA2 Protein/metabolism ; Humans ; Interleukin-1alpha/genetics ; Interleukin-1alpha/metabolism ; Mesenchymal Stem Cells/metabolism ; Myocytes, Cardiac/metabolism ; Oxidative Stress ; Proteome ; Proteomics ; RNA-Binding Proteins/genetics ; RNA-Binding Proteins/metabolism ; Receptor-Like Protein Tyrosine Phosphatases, Class 2/genetics ; Receptor-Like Protein Tyrosine Phosphatases, Class 2/metabolism ; Signal Transduction
Chemical Substances	HMGA2 Protein ; HMGA2 protein, human ; IGF2BP3 protein, human ; IL1A protein, human ; Interleukin-1alpha ; Proteome ; RNA-Binding Proteins ; PTPRF protein, human (EC 3.1.3.48) ; Receptor-Like Protein Tyrosine Phosphatases, Class 2 (EC 3.1.3.48)
Language	English
Publishing date	2022-01-07
Publishing country	England
Document type	Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-021-03956-8
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Enhanced cross-recognition of SARS-CoV-2 Omicron variant by peptide vaccine-induced antibodies.

Aparicio, Belén / Ruiz, Marta / Casares, Noelia / Silva, Leyre / Egea, Josune / Pérez, Patricia / Albericio, Guillermo / Esteban, Mariano / García-Arriaza, Juan / Lasarte, Juan J / Sarobe, Pablo

Frontiers in immunology

2023 Volume 13, Page(s) 1044025

Abstract: Current vaccines against SARS-CoV-2, based on the original Wuhan sequence, induce antibodies with different degrees of cross-recognition of new viral variants of concern. Despite potent responses generated in vaccinated and infected individuals, the ... ...

Abstract	Current vaccines against SARS-CoV-2, based on the original Wuhan sequence, induce antibodies with different degrees of cross-recognition of new viral variants of concern. Despite potent responses generated in vaccinated and infected individuals, the Omicron (B.1.1.529) variant causes breakthrough infections, facilitating viral transmission. We previously reported a vaccine based on a cyclic peptide containing the 446-488 S1 sequence (446-488cc) of the SARS-CoV-2 spike (S) protein from Wuhan isolate. To provide the best immunity against Omicron, here we compared Omicron-specific immunity induced by a Wuhan-based 446-488cc peptide, by a Wuhan-based recombinant receptor-binding domain (RBD) vaccine and by a new 446-488cc peptide vaccine based on the Omicron sequence. Antibodies induced by Wuhan peptide 446-488cc in three murine strains not only recognized the Wuhan and Omicron 446-488 peptides similarly, but also Wuhan and Omicron RBD protein variants. By contrast, antibodies induced by the Wuhan recombinant RBD vaccine showed a much poorer cross-reactivity for the Omicron RBD despite similar recognition of Wuhan and Omicron peptide variants. Finally, although the Omicron-based 446-488cc peptide vaccine was poorly immunogenic in mice due to the loss of T cell epitopes, co-immunization with Omicron peptide 446-488cc and exogenous T cell epitopes induced strong cross-reactive antibodies that neutralized Omicron SARS-CoV-2 virus. Since mutations occurring within this sequence do not alter T cell epitopes in humans, these results indicate the robust immunogenicity of 446-488cc-based peptide vaccines that induce antibodies with a high cross-recognition capacity against Omicron, and suggest that this sequence could be included in future vaccines targeting the Omicron variant.
MeSH term(s)	Humans ; Animals ; Mice ; SARS-CoV-2 ; COVID-19 Vaccines ; Epitopes, T-Lymphocyte ; COVID-19/prevention & control ; Vaccines, Subunit ; Antibodies
Chemical Substances	COVID-19 Vaccines ; Epitopes, T-Lymphocyte ; Vaccines, Subunit ; Antibodies
Language	English
Publishing date	2023-01-24
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.1044025
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Article ; Online: Erratum: Enhanced cross-recognition of SARS-CoV-2 Omicron variant by peptide vaccine-induced antibodies.

Aparicio, Belén / Ruiz, Marta / Casares, Noelia / Silva, Leyre / Egea, Josune / Pérez, Patricia / Albericio, Guillermo / Esteban, Mariano / García-Arriaza, Juan / Lasarte, Juan J / Sarobe, Pablo

Frontiers in immunology

2023 Volume 14, Page(s) 1172427

Abstract: This corrects the article DOI: 10.3389/fimmu.2022.1044025.]. ...

Abstract	[This corrects the article DOI: 10.3389/fimmu.2022.1044025.].
Language	English
Publishing date	2023-03-03
Publishing country	Switzerland
Document type	Published Erratum
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1172427
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Article ; Online: Highly Attenuated Poxvirus-Based Vaccines Against Emerging Viral Diseases.

Perdiguero, Beatriz / Pérez, Patricia / Marcos-Villar, Laura / Albericio, Guillermo / Astorgano, David / Álvarez, Enrique / Sin, Laura / Gómez, Carmen Elena / García-Arriaza, Juan / Esteban, Mariano

Journal of molecular biology

2023 Volume 435, Issue 15, Page(s) 168173

Abstract	Although one member of the poxvirus family, variola virus, has caused one of the most devastating human infections worldwide, smallpox, the knowledge gained over the last 30 years on the molecular, virological and immunological mechanisms of these viruses has allowed the use of members of this family as vectors for the generation of recombinant vaccines against numerous pathogens. In this review, we cover different aspects of the history and biology of poxviruses with emphasis on their application as vaccines, from first- to fourth-generation, against smallpox, monkeypox, emerging viral diseases highlighted by the World Health Organization (COVID-19, Crimean-Congo haemorrhagic fever, Ebola and Marburg virus diseases, Lassa fever, Middle East respiratory syndrome and severe acute respiratory syndrome, Nipah and other henipaviral diseases, Rift Valley fever and Zika), as well as against one of the most concerning prevalent virus, the Human Immunodeficiency Virus, the causative agent of Acquired Immunodeficiency Syndrome. We discuss the implications in human health of the 2022 monkeypox epidemic affecting many countries, and the rapid prophylactic and therapeutic measures adopted to control virus dissemination within the human population. We also describe the preclinical and clinical evaluation of the Modified Vaccinia virus Ankara and New York vaccinia virus poxviral strains expressing heterologous antigens from the viral diseases listed above. Finally, we report different approaches to improve the immunogenicity and efficacy of poxvirus-based vaccine candidates, such as deletion of immunomodulatory genes, insertion of host-range genes and enhanced transcription of foreign genes through modified viral promoters. Some future prospects are also highlighted.
MeSH term(s)	Animals ; Humans ; Communicable Diseases, Emerging/prevention & control ; Communicable Diseases, Emerging/virology ; COVID-19/prevention & control ; Genetic Vectors ; Mpox (monkeypox)/prevention & control ; Poxviridae/immunology ; Smallpox/prevention & control ; Vaccines, Attenuated ; Vaccinia virus/genetics ; Viral Vaccines/genetics ; Viral Vaccines/immunology ; Virus Diseases/prevention & control ; Virus Diseases/virology ; Zika Virus ; Zika Virus Infection
Chemical Substances	Vaccines, Attenuated ; Viral Vaccines
Language	English
Publishing date	2023-06-08
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	80229-3
ISSN	1089-8638 ; 0022-2836
ISSN (online)	1089-8638
ISSN	0022-2836
DOI	10.1016/j.jmb.2023.168173
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Lung transcriptomics of K18-hACE2 mice highlights mechanisms and genes involved in the MVA-S vaccine-mediated immune response and protection against SARS-CoV-2 infection.

Gómez-Carballa, Alberto / Albericio, Guillermo / Montoto-Louzao, Julián / Pérez, Patricia / Astorgano, David / Rivero-Calle, Irene / Martinón-Torres, Federico / Esteban, Mariano / Salas, Antonio / García-Arriaza, Juan

Antiviral research

2023 Volume 220, Page(s) 105760

Abstract: Unravelling the molecular mechanism of COVID-19 vaccines through transcriptomic pathways involved in the host response to SARS-CoV-2 infection is key to understand how vaccines work, and for the development of optimized COVID-19 vaccines that can prevent ...

Abstract	Unravelling the molecular mechanism of COVID-19 vaccines through transcriptomic pathways involved in the host response to SARS-CoV-2 infection is key to understand how vaccines work, and for the development of optimized COVID-19 vaccines that can prevent the emergence of SARS-CoV-2 variants of concern (VoCs) and future outbreaks. In this study, we investigated the effects of vaccination with a modified vaccinia virus Ankara (MVA)-based vector expressing the full-length SARS-CoV-2 spike protein (MVA-S) on the lung transcriptome from susceptible K18-hACE2 mice after SARS-CoV-2 infection. One dose of MVA-S regulated genes related to viral infection control, inflammation processes, T-cell response, cytokine production and IFN-γ signalling. Down-regulation of Rhcg and Tnfsf18 genes post-vaccination with one and two doses of MVA-S may represent a mechanism for controlling infection immunity and vaccine-induced protection. One dose of MVA-S provided partial protection with a distinct lung transcriptomic profile to healthy animals, while two doses of MVA-S fully protected against infection with a transcriptomic profile comparable to that of non-vaccinated healthy animals. This suggests that the MVA-S booster generates a robust and rapid antigen-specific immune response preventing virus infection. Notably, down-regulation of Atf3 and Zbtb16 genes in mice vaccinated with two doses of MVA-S may contribute to vaccine control of innate immune system and inflammation processes in the lungs during SARS-CoV-2 infection. This study shows host transcriptomic mechanisms likely involved in the MVA-S vaccine-mediated immune response against SARS-CoV-2 infection, which could help in improving vaccine dose assessment and developing novel, well-optimized SARS-CoV-2 vaccine candidates against prevalent or emerging VoCs.
MeSH term(s)	Humans ; Animals ; Mice ; Vaccinia virus/genetics ; COVID-19 Vaccines/genetics ; Antibodies, Viral ; COVID-19/prevention & control ; SARS-CoV-2/genetics ; Vaccines ; Gene Expression Profiling ; Immunity ; Lung ; Inflammation
Chemical Substances	spike protein, SARS-CoV-2 ; COVID-19 Vaccines ; K-18 conjugate ; Antibodies, Viral ; Vaccines
Language	English
Publishing date	2023-11-21
Publishing country	Netherlands
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	306628-9
ISSN	1872-9096 ; 0166-3542
ISSN (online)	1872-9096
ISSN	0166-3542
DOI	10.1016/j.antiviral.2023.105760
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Article ; Online: Preclinical immune efficacy against SARS-CoV-2 beta B.1.351 variant by MVA-based vaccine candidates.

Pérez, Patricia / Albericio, Guillermo / Astorgano, David / Flores, Sara / Sánchez-Corzo, Cristina / Sánchez-Cordón, Pedro J / Luczkowiak, Joanna / Delgado, Rafael / Casasnovas, José M / Esteban, Mariano / García-Arriaza, Juan

Frontiers in immunology

2023 Volume 14, Page(s) 1264323

Abstract: The constant appearance of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VoCs) has jeopardized the protective capacity of approved vaccines against coronavirus disease-19 (COVID-19). For this reason, the generation ...

Abstract	The constant appearance of new severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants of concern (VoCs) has jeopardized the protective capacity of approved vaccines against coronavirus disease-19 (COVID-19). For this reason, the generation of new vaccine candidates adapted to the emerging VoCs is of special importance. Here, we developed an optimized COVID-19 vaccine candidate using the modified vaccinia virus Ankara (MVA) vector to express a full-length prefusion-stabilized SARS-CoV-2 spike (S) protein, containing 3 proline (3P) substitutions in the S protein derived from the beta (B.1.351) variant, termed MVA-S(3Pbeta). Preclinical evaluation of MVA-S(3Pbeta) in head-to-head comparison to the previously generated MVA-S(3P) vaccine candidate, expressing a full-length prefusion-stabilized Wuhan S protein (with also 3P substitutions), demonstrated that two intramuscular doses of both vaccine candidates fully protected transgenic K18-hACE2 mice from a lethal challenge with SARS-CoV-2 beta variant, reducing mRNA and infectious viral loads in the lungs and in bronchoalveolar lavages, decreasing lung histopathological lesions and levels of proinflammatory cytokines in the lungs. Vaccination also elicited high titers of anti-S Th1-biased IgGs and neutralizing antibodies against ancestral SARS-CoV-2 Wuhan strain and VoCs alpha, beta, gamma, delta, and omicron. In addition, similar systemic and local SARS-CoV-2 S-specific CD4
MeSH term(s)	Mice ; Animals ; Humans ; SARS-CoV-2/genetics ; Vaccinia virus/genetics ; COVID-19 Vaccines ; Antibodies, Viral ; COVID-19/prevention & control ; Mice, Inbred C57BL ; Vaccines
Chemical Substances	COVID-19 Vaccines ; Antibodies, Viral ; Vaccines
Language	English
Publishing date	2023-12-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1264323
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Article ; Online: Optimized vaccine candidate MVA-S(3P) fully protects against SARS-CoV-2 infection in hamsters.

Abdelnabi, Rana / Pérez, Patricia / Astorgano, David / Albericio, Guillermo / Kerstens, Winnie / Thibaut, Hendrik Jan / Coelmont, Lotte / Weynand, Birgit / Labiod, Nuria / Delgado, Rafael / Montenegro, Dolores / Puentes, Eugenia / Rodríguez, Esteban / Neyts, Johan / Dallmeier, Kai / Esteban, Mariano / García-Arriaza, Juan

Frontiers in immunology

2023 Volume 14, Page(s) 1163159

Abstract: The development of novel optimized vaccines against coronavirus disease 2019 (COVID-19) that are capable of controlling the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic and the appearance of different variants of concern (VoC) is ...

Abstract	The development of novel optimized vaccines against coronavirus disease 2019 (COVID-19) that are capable of controlling the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic and the appearance of different variants of concern (VoC) is needed to fully prevent the transmission of the virus. In the present study, we describe the enhanced immunogenicity and efficacy elicited in hamsters by a modified vaccinia virus Ankara (MVA) vector expressing a full-length prefusion-stabilized SARS-CoV-2 spike (S) protein [termed MVA-S(3P)]. Hamsters vaccinated with one or two doses of MVA-S(3P) developed high titers of S-binding IgG antibodies and neutralizing antibodies against the ancestral Wuhan SARS-CoV-2 virus and VoC beta, gamma, and delta, as well as against omicron, although with a somewhat lower neutralization activity. After SARS-CoV-2 challenge, vaccinated hamsters did not lose body weight as compared to matched placebo (MVA-WT) controls. Consistently, vaccinated hamsters exhibited significantly reduced viral RNA in the lungs and nasal washes, and no infectious virus was detected in the lungs in comparison to controls. Furthermore, almost no lung histopathology was detected in MVA-S(3P)-vaccinated hamsters, which also showed significantly reduced levels of proinflammatory cytokines in the lungs compared to unvaccinated hamsters. These results reinforce the use of MVA-S(3P) as a vaccine candidate against COVID-19 in clinical trials.
MeSH term(s)	Animals ; Cricetinae ; COVID-19/prevention & control ; SARS-CoV-2 ; Vaccinia virus/genetics ; Antibodies, Neutralizing
Chemical Substances	Antibodies, Neutralizing
Language	English
Publishing date	2023-10-18
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2023.1163159
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Article ; Online: Intranasal administration of a single dose of MVA-based vaccine candidates against COVID-19 induced local and systemic immune responses and protects mice from a lethal SARS-CoV-2 infection.

Pérez, Patricia / Astorgano, David / Albericio, Guillermo / Flores, Sara / Sánchez-Cordón, Pedro J / Luczkowiak, Joanna / Delgado, Rafael / Casasnovas, José M / Esteban, Mariano / García-Arriaza, Juan

Frontiers in immunology

2022 Volume 13, Page(s) 995235

Abstract: Current coronavirus disease-19 (COVID-19) vaccines are administered by the intramuscular route, but this vaccine administration failed to prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus infection in the upper respiratory tract, ...

Abstract	Current coronavirus disease-19 (COVID-19) vaccines are administered by the intramuscular route, but this vaccine administration failed to prevent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus infection in the upper respiratory tract, mainly due to the absence of virus-specific mucosal immune responses. It is hypothesized that intranasal (IN) vaccination could induce both mucosal and systemic immune responses that blocked SARS-CoV-2 transmission and COVID-19 progression. Here, we evaluated in mice IN administration of three modified vaccinia virus Ankara (MVA)-based vaccine candidates expressing the SARS-CoV-2 spike (S) protein, either the full-length native S or a prefusion-stabilized [S(3P)] protein; SARS-CoV-2-specific immune responses and efficacy were determined after a single IN vaccine application. Results showed that in C57BL/6 mice, MVA-based vaccine candidates elicited S-specific IgG and IgA antibodies in serum and bronchoalveolar lavages, respectively, and neutralizing antibodies against parental and SARS-CoV-2 variants of concern (VoC), with MVA-S(3P) being the most immunogenic vaccine candidate. IN vaccine administration also induced polyfunctional S-specific Th1-skewed CD4
MeSH term(s)	Administration, Intranasal ; Animals ; Antibodies, Neutralizing ; Antibodies, Viral ; Antibody Formation ; COVID-19/prevention & control ; COVID-19 Vaccines ; Cytokines ; Immunoglobulin A ; Immunoglobulin G ; Mice ; Mice, Inbred C57BL ; SARS-CoV-2 ; Vaccinia virus/genetics ; Viral Vaccines
Chemical Substances	Antibodies, Neutralizing ; Antibodies, Viral ; COVID-19 Vaccines ; Cytokines ; Immunoglobulin A ; Immunoglobulin G ; Viral Vaccines
Language	English
Publishing date	2022-09-12
Publishing country	Switzerland
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2606827-8
ISSN	1664-3224 ; 1664-3224
ISSN (online)	1664-3224
ISSN	1664-3224
DOI	10.3389/fimmu.2022.995235
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Article: The Vascular Niche for Adult Cardiac Progenitor Cells.

Herrero, Diego / Albericio, Guillermo / Higuera, Marina / Herranz-López, María / García-Brenes, Miguel A / Cordero, Alejandra / Roche, Enrique / Sepúlveda, Pilar / Mora, Carmen / Bernad, Antonio

Antioxidants (Basel, Switzerland)

2022 Volume 11, Issue 5

Abstract: Research on cardiac progenitor cell populations has generated expectations about their potential for cardiac regeneration capacity after acute myocardial infarction and during physiological aging; however, the endogenous capacity of the adult mammalian ... ...

Abstract	Research on cardiac progenitor cell populations has generated expectations about their potential for cardiac regeneration capacity after acute myocardial infarction and during physiological aging; however, the endogenous capacity of the adult mammalian heart is limited. The modest efficacy of exogenous cell-based treatments can guide the development of new approaches that, alone or in combination, can be applied to boost clinical efficacy. The identification and manipulation of the adult stem cell environment, termed niche, will be critical for providing new evidence on adult stem cell populations and improving stem-cell-based therapies. Here, we review and discuss the state of our understanding of the interaction of adult cardiac progenitor cells with other cardiac cell populations, with a focus on the description of the B-CPC progenitor population (Bmi1+ cardiac progenitor cell), which is a strong candidate progenitor for all main cardiac cell lineages, both in the steady state and after cardiac damage. The set of all interactions should be able to define the vascular cardiac stem cell niche, which is associated with low oxidative stress domains in vasculature, and whose manipulation would offer new hope in the cardiac regeneration field.
Language	English
Publishing date	2022-04-29
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2704216-9
ISSN	2076-3921
ISSN	2076-3921
DOI	10.3390/antiox11050882
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