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  2. AU="Yin, Xiaohong"

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  1. Article: Olive oil authentication: A comparative analysis of regulatory frameworks with especial emphasis on quality and authenticity indices, and recent analytical techniques developed for their assessment. A review

    Bajoub, Aadil / Alessandra Bendini / Alberto Fernández-Gutiérrez / Alegría Carrasco-Pancorbo

    Critical reviews in food science and nutrition. 2018 Mar. 24, v. 58, no. 5

    2018  

    Abstract: Over the last decades, olive oil quality and authenticity control has become an issue of great importance to consumers, suppliers, retailers, and regulators in both traditional and emerging olive oil producing countries, mainly due to the increasing ... ...

    Abstract Over the last decades, olive oil quality and authenticity control has become an issue of great importance to consumers, suppliers, retailers, and regulators in both traditional and emerging olive oil producing countries, mainly due to the increasing worldwide popularity and the trade globalization of this product. Thus, in order to ensure olive oil authentication, various national and international laws and regulations have been adopted, although some of them are actually causing an enormous debate about the risk that they can represent for the harmonization of international olive oil trade standards. Within this context, this review was designed to provide a critical overview and comparative analysis of selected regulatory frameworks for olive oil authentication, with special emphasis on the quality and purity criteria considered by these regulation systems, their thresholds and the analytical methods employed for monitoring them. To complete the general overview, recent analytical advances to overcome drawbacks and limitations of the official methods to evaluate olive oil quality and to determine possible adulterations were reviewed. Furthermore, the latest trends on analytical approaches to assess the olive oil geographical and varietal origin traceability were also examined.
    Keywords analytical methods ; globalization ; laws and regulations ; monitoring ; olive oil ; product authenticity ; risk ; traceability ; trade
    Language English
    Dates of publication 2018-0324
    Size p. 832-857.
    Publishing place Taylor & Francis
    Document type Article
    ZDB-ID 1037504-1
    ISSN 1549-7852 ; 1040-8398
    ISSN (online) 1549-7852
    ISSN 1040-8398
    DOI 10.1080/10408398.2016.1225666
    Database NAL-Catalogue (AGRICOLA)

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  2. Article: Leaf removal at veraison stage differentially affects qualitative attributes and bioactive composition of fresh and dehydrated grapes of two indigenous Cypriot cultivars

    Constantinou, Savvas / Alberto Fernandez‐Gutierrez / Ana Maria Gómez‐Caravaca / George A Manganaris / Stefanos Koundouras / Vlasios Goulas

    Journal of the science of food and agriculture. 2019 Feb., v. 99, no. 3

    2019  

    Abstract: BACKGROUND: The effect of leaf removal on postharvest performance of dehydrated grapes has been poorly analyzed. The aim of this study was to evaluate the effect of leaf removal at veraison stage on the metabolites of fresh and dehydrated grapes of two ... ...

    Abstract BACKGROUND: The effect of leaf removal on postharvest performance of dehydrated grapes has been poorly analyzed. The aim of this study was to evaluate the effect of leaf removal at veraison stage on the metabolites of fresh and dehydrated grapes of two indigenous Cypriot cultivars (‘Mavro’ and ‘Xynisteri’), which are destined for the production of ‘Commandaria’, a protected designation of origin (PDO) premium sweet wine. RESULTS: Leaf removal led to a reduction of soluble solids, titratable acidity, aroma potential and most of the phenolic groups in the musts of both cultivars. Dehydration led to a significant increase in all of these parameters in both cultivars, being more pronounced in cv. ‘Mavro’. Interestingly, leaf removal indicated differential response in the dehydrated product of the cultivars examined; liquid chromatography (LC‐DAD‐qTOF‐MS) data showed a significant decrease in phenolic compounds in ‘Xynisteri’ must (from 66.73 to 44.15 mg L−1), while ‘Mavro’ must registered similar values of phenolic compounds (from 94.78 to 96.72 mg L−1), but with a different distribution among phenolic groups. Intriguingly, flavonols and flavan‐3‐ols that present significant health‐promoting properties showed higher concentrations in the must from dehydrated ‘Mavro’ grapes that were subjected to preharvest leaf removal. CONCLUSIONS: Leaf removal at veraison stage followed by sun drying differentially affected the chemical composition of the examined cultivars. This preharvest application followed by postharvest dehydration was beneficial for cv. ‘Mavro’, while this was not the case for cv. ‘Xynisteri’. The effects of leaf removal in other developmental stages (i.e. pre/post bloom stage) need to be dissected with the ultimate goal of providing an end product with high bioactive content. © 2018 Society of Chemical Industry
    Keywords cultivars ; developmental stages ; flavanols ; flavonols ; grapes ; health promotion ; leaves ; liquid chromatography ; metabolites ; odors ; phenolic compounds ; preharvest treatment ; protected designation of origin ; solar drying ; titratable acidity ; total soluble solids ; wines
    Language English
    Dates of publication 2019-02
    Size p. 1342-1350.
    Publishing place John Wiley & Sons, Ltd
    Document type Article
    Note JOURNAL ARTICLE
    ZDB-ID 184116-6
    ISSN 1097-0010 ; 0022-5142
    ISSN (online) 1097-0010
    ISSN 0022-5142
    DOI 10.1002/jsfa.9309
    Database NAL-Catalogue (AGRICOLA)

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  3. Article ; Online: Potential of LC Coupled to Fluorescence Detection in Food Metabolomics

    Romina P. Monasterio / Lucía Olmo-García / Aadil Bajoub / Alberto Fernández-Gutiérrez / Alegría Carrasco-Pancorbo

    International Journal of Molecular Sciences, Vol 17, Iss 10, p

    Determination of Phenolic Compounds in Virgin Olive Oil

    2016  Volume 1627

    Abstract: A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An ... ...

    Abstract A powerful chromatographic method coupled to a fluorescence detector was developed to determine the phenolic compounds present in virgin olive oil (VOO), with the aim to propose an appropriate alternative to liquid chromatography-mass spectrometry. An excitation wavelength of 285 nm was selected and four different emission wavelengths (316, 328, 350 and 450 nm) were simultaneously recorded, working therefore on “multi-emission” detection mode. With the use of commercially available standards and other standards obtained by semipreparative high performance liquid chromatography, it was possible to identify simple phenols, lignans, several complex phenols, and other phenolic compounds present in the matrix under study. A total of 26 phenolic compounds belonging to different chemical families were identified (23 of them were susceptible of being quantified). The proposed methodology provided detection and quantification limits within the ranges of 0.004–7.143 μg·mL−1 and 0.013–23.810 μg·mL−1, respectively. As far as the repeatability is concerned, the relative standard deviation values were below 0.43% for retention time, and 9.05% for peak area. The developed methodology was applied for the determination of phenolic compounds in ten VOOs, both monovarietals and blends. Secoiridoids were the most abundant fraction in all the samples, followed by simple phenolic alcohols, lignans, flavonoids, and phenolic acids (being the abundance order of the latter chemical classes logically depending on the variety and origin of the VOOs).
    Keywords fluorescence detection ; olive oil ; phenolic compounds ; secoiridoids ; food metabolomics ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2016-09-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  4. Article: A multifunctional material based on co-electrospinning for developing biosensors with optical oxygen transduction

    Ramon-Marquez, Teresa / Alberto Fernandez-Gutierrez / Antonio L. Medina-Castillo / Jorge F. Fernandez-Sanchez / Naveen Nagiah

    Analytica chimica acta. 2018 July 26, v. 1015

    2018  

    Abstract: A multifunctional material based on co-electrospinning has been developed as a basic material for the development of biosensors with optical oxygen transduction. It is based on coaxial nanofibres: inner fibres containing an oxygen sensitive dye and outer ...

    Abstract A multifunctional material based on co-electrospinning has been developed as a basic material for the development of biosensors with optical oxygen transduction. It is based on coaxial nanofibres: inner fibres containing an oxygen sensitive dye and outer fibres containing aldehyde groups to allow the formation of Schiff bases with the amino groups of the enzyme. The resulting material preserves the oxygen sensing properties of the inner optical transducer as well as exhibits a high capacity for immobilizing molecules on its surface.Uricase has been selected as model enzyme and several parameters (temperature, pH, reaction time, buffer, and enzyme concentration) have been optimised to demonstrate the versatility of this novel multifunctional material in the development of biosensors with optical oxygen transduction for determining uric acid in serum samples. It suggests that the proposed multifunctional material can provide a promising multifunctional platform for biosensing applications.
    Keywords aldehydes ; analytical chemistry ; biosensors ; blood serum ; models ; nanofibers ; oxygen ; pH ; schiff bases ; temperature ; transducers (equipment) ; uric acid
    Language English
    Dates of publication 2018-0726
    Size p. 66-73.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 1483436-4
    ISSN 1873-4324 ; 0003-2670
    ISSN (online) 1873-4324
    ISSN 0003-2670
    DOI 10.1016/j.aca.2018.02.010
    Database NAL-Catalogue (AGRICOLA)

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  5. Article: Development of a folic acid molecularly imprinted polymer and its evaluation as a sorbent for dispersive solid-phase extraction by liquid chromatography coupled to mass spectrometry

    Panjan, Peter / Adama M. Sesay / Alberto Fernandez-Gutierrez / Alegría Carrasco-Pancorbo / Jorge F. Fernandez-Sanchez / Romina P. Monasterio

    Journal of chromatography. 2018,

    2018  

    Abstract: This work shows the development of a molecularly imprinted polymer to determine folic acid (FA) in food extracts by using dispersive solid-phase extraction and liquid chromatography coupled to mass spectrometry (LC–MS). Herewith, combinations of monomers ...

    Abstract This work shows the development of a molecularly imprinted polymer to determine folic acid (FA) in food extracts by using dispersive solid-phase extraction and liquid chromatography coupled to mass spectrometry (LC–MS). Herewith, combinations of monomers (methacrylic acid (MAA), 4-vinylpyridine (4VPy) and vinylbenzyl trimethylammonium chloride (VBTMAC)) and crosslinkers (ethylene glycol dimethacrylate (EGDMA) and divinyl benzene (DVB)) were tested in appropriate solvents. Isotherm tests revealed that the MIP with the highest affinity was obtained by combining VBTMAC and EGDMA. Having checked the appropriate template-monomer-crosslinker ratio, the FA MIP was analyzed for its kinetic and equilibrium binding properties, proving very high affinity (more than 2.5 mmol g−1) and MIP/NIP ratio (up to 37). The FA MIP was used to selectively isolate the compound of interest from lettuce and cookies matrices using a dispersive solid-phase extraction protocol (which exhibited appropriate recovery and repeatability, ≥79.50% and ≤13.41 (%RSD in terms of area values), respectively, as well as absence of matrix effect); the resulting extracts were analyzed by a rapid and reliable LC–MS method.
    Keywords binding properties ; cookies ; folic acid ; lettuce ; liquid chromatography ; mass spectrometry ; polymers ; protocols ; solid phase extraction ; solvents ; sorbents
    Language English
    Size p. .
    Publishing place Elsevier B.V.
    Document type Article
    Note Pre-press version
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2018.09.037
    Database NAL-Catalogue (AGRICOLA)

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  6. Article: Evaluating the potential of phenolic profiles as discriminant features among extra virgin olive oils from Moroccan controlled designations of origin

    Bajoub, Aadil / Alberto Fernández-Gutiérrez / Alegría Carrasco-Pancorbo / El Amine Ajal

    Food research international. 2016 June, v. 84

    2016  

    Abstract: Herewith, the potential of an approach based on the combination of the determination of phenolic compounds and the use of chemometric treatments has been evaluated to establish, for the first time, promising models to authenticate the provenance of ... ...

    Abstract Herewith, the potential of an approach based on the combination of the determination of phenolic compounds and the use of chemometric treatments has been evaluated to establish, for the first time, promising models to authenticate the provenance of Moroccan monovarietal olive oils produced under different geographical origin indication systems. To achieve this purpose, 136 commercial extra virgin olive oil samples from three diverse production areas (Meknès territory; the Protected Geographical Indication Ouazzane; and the Protected Designation of Origin Tyout-Chiadma) were collected over two consecutive crop seasons (2012/2013 and 2013/2014). Their phenolic fraction composition was investigated by using high performance liquid chromatography coupled to mass spectrometry (HPLC-ESI-IT MS). The results showed that geographical provenance and harvest season had a marked influence on the content of identified phenolic compounds. Principal components analysis (PCA) and linear discriminant analysis (LDA) were applied to test the potential of the determined compounds as geographical discriminant features, achieving a noticeable discrimination among the three evaluated regions. The contribution of each analyte to the statistic model has been evaluated in depth.
    Keywords chemometrics ; discriminant analysis ; extra-virgin olive oil ; harvest date ; high performance liquid chromatography ; mass spectrometry ; models ; phenolic compounds ; principal component analysis ; protected designation of origin ; provenance
    Language English
    Dates of publication 2016-06
    Size p. 41-51.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2016.03.010
    Database NAL-Catalogue (AGRICOLA)

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  7. Article: Evaluating the reliability of specific and global methods to assess the phenolic content of virgin olive oil: Do they drive to equivalent results?

    Olmo-García, Lucía / Alberto Fernández-Gutiérrez / Alegría Carrasco-Pancorbo / Ana Hidalgo / Carmen Fernández-Fernández / Pedro Vílchez / Rosa Marchal

    Journal of chromatography. 2019 Jan. 25, v. 1585

    2019  

    Abstract: Despite the huge number of different published methodologies, there is an open debate regarding which one is the most convenient analytical strategy for the determination of phenolic compounds from virgin olive oils. Diverse technical issues together ... ...

    Abstract Despite the huge number of different published methodologies, there is an open debate regarding which one is the most convenient analytical strategy for the determination of phenolic compounds from virgin olive oils. Diverse technical issues together with the disparity of criteria regarding results expression cause a lot of confusion. Herewith, a systematic comparison between specific (a powerful and LC–MS method) and global methodologies (the Folin-Ciocalteau (FC) colorimetric assay, the International Olive Council (IOC) method and hydrolysis plus HPLC-DAD) has been carried out. Thus, these strategies have been applied to the analysis of 50 extra virgin olive oils (covering all the possible quantitative ranges of these substances). This is the first time in which the individual LC–MS quantification of so many phenolic substances is included in this kind of comprehensive comparison. The outcomes of all the strategies have been thoroughly confronted and their equivalence (or divergence) has been carefully evaluated, establishing possible correspondence factors.The LC–MS individual determination with the pure standard of every analyte represented the ideal situation; when only the commercially available standards were used, a drastic change was observed in the absolute concentrations of oleuropein derivatives (in terms of hydroxytyrosol). Total phenolic content (summing individual levels) proved to be higher (1.9–3.0 times when data was expressed in mg/kg) than the values given by the three non-specific methods (with R2 from 0.84 to 0.90). In any case, the IOC method, the FC assay and the hydrolysis approach could be considered as feasible strategies when a global value is pursued. Good correlations between their results were found (R2 > 0.89), with the following equivalence factors: FC(mg caffeic acid/kg) ≈ 0.60 IOC(mg TY/kg); IOC(mg TY/kg) ≈ 1.27 Sum acid hydrolysis(mg TY+HTY/kg); FC(mg HTY/kg) ≈ 1.04 Sum acid hydrolysis(mg TY+HTY/kg).
    Keywords acid hydrolysis ; caffeic acid ; colorimetry ; extra-virgin olive oil ; high performance liquid chromatography ; mass spectrometry ; oleuropein
    Language English
    Dates of publication 2019-0125
    Size p. 56-69.
    Publishing place Elsevier B.V.
    Document type Article
    ZDB-ID 218139-3
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    ISSN 0021-9673 ; 0378-4355 ; 0376-737X
    DOI 10.1016/j.chroma.2018.11.031
    Database NAL-Catalogue (AGRICOLA)

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  8. Article ; Online: Health Effects of Psidium guajava L. Leaves

    Elixabet Díaz-de-Cerio / Vito Verardo / Ana María Gómez-Caravaca / Alberto Fernández-Gutiérrez / Antonio Segura-Carretero

    International Journal of Molecular Sciences, Vol 18, Iss 4, p

    An Overview of the Last Decade

    2017  Volume 897

    Abstract: Today, there is increasing interest in discovering new bioactive compounds derived from ethnomedicine. Preparations of guava (Psidium guajava L.) leaves have traditionally been used to manage several diseases. The pharmacological research in vitro as ... ...

    Abstract Today, there is increasing interest in discovering new bioactive compounds derived from ethnomedicine. Preparations of guava (Psidium guajava L.) leaves have traditionally been used to manage several diseases. The pharmacological research in vitro as well as in vivo has been widely used to demonstrate the potential of the extracts from the leaves for the co-treatment of different ailments with high prevalence worldwide, upholding the traditional medicine in cases such as diabetes mellitus, cardiovascular diseases, cancer, and parasitic infections. Moreover, the biological activity has been attributed to the bioactive composition of the leaves, to some specific phytochemical subclasses, or even to individual compounds. Phenolic compounds in guava leaves have been credited with regulating blood-glucose levels. Thus, the aim of the present review was to compile results from in vitro and in vivo studies carried out with guava leaves over the last decade, relating the effects to their clinical applications in order to focus further research for finding individual bioactive compounds. Some food applications (guava tea and supplementary feed for aquaculture) and some clinical, in vitro, and in vivo outcomes are also included.
    Keywords Psidium guajava L. (guava) leaves ; traditional medicine ; in vitro ; in vivo ; phenolic compounds ; pharmacology ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2017-04-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  9. Article ; Online: Comparison of Two Stationary Phases for the Determination of Phytosterols and Tocopherols in Mango and Its By-Products by GC-QTOF-MS

    Ana López-Cobo / Beatriz Martín-García / Antonio Segura-Carretero / Alberto Fernández-Gutiérrez / Ana María Gómez-Caravaca

    International Journal of Molecular Sciences, Vol 18, Iss 7, p

    2017  Volume 1594

    Abstract: Two different gas chromatography coupled to quadrupole-time of flight mass spectrometry (GC-QTOF-MS) methodologies were carried out for the analysis of phytosterols and tocopherols in the flesh of three mango cultivars and their by-products (pulp, peel, ... ...

    Abstract Two different gas chromatography coupled to quadrupole-time of flight mass spectrometry (GC-QTOF-MS) methodologies were carried out for the analysis of phytosterols and tocopherols in the flesh of three mango cultivars and their by-products (pulp, peel, and seed). To that end, a non-polar column ((5%-phenyl)-methylpolysiloxane (HP-5ms)) and a mid-polar column (crossbond trifluoropropylmethyl polysiloxane (RTX-200MS)) were used. The analysis time for RTX-200MS was much lower than the one obtained with HP-5ms. Furthermore, the optimized method for the RTX-200MS column had a higher sensibility and precision of peak area than the HP-5ms methodology. However, RTX-200MS produced an overlapping between β-sitosterol and Δ5-avenasterol. Four phytosterols and two tocopherols were identified in mango samples. As far as we are concerned, this is the first time that phytosterols have been studied in mango peel and that Δ5-avenasterol has been reported in mango pulp. α- and γ-tocopherol were determined in peel, and α-tocopherol was the major tocopherol in this fraction (up to 81.2%); however, only α-tocopherol was determined in the pulp and seed. The peel was the fraction with the highest total concentration of phytosterols followed by seed and pulp, and “Sensación” was the cultivar with the highest concentration of total phytosterols in most cases. There were no significant differences between quantification of tocopherols with both columns. However, in most cases, quantification of phytosterols was higher with RTX-200MS than with HP-5ms column.
    Keywords mango ; GC-QTOF-MS ; phytosterols ; tocopherols ; by-product ; unsaponifiable ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
    Subject code 540
    Language English
    Publishing date 2017-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  10. Article: UHPLC/MS2-based approach for the comprehensive metabolite profiling of bean (Vicia faba L.) by-products: A promising source of bioactive constituents

    Abu-Reidah, Ibrahim M / Alberto Fernández-Gutiérrez / Antonio Segura-Carretero / David Arráez-Román / Ismail Warad

    Food research international. 2017 Mar., v. 93

    2017  

    Abstract: Today, by-products generated from the agro-industrial practices are considered a key source of bioactive and functional components, that can be used for their nutritional and added value properties. New aspects concerning the use of these wastes as by- ... ...

    Abstract Today, by-products generated from the agro-industrial practices are considered a key source of bioactive and functional components, that can be used for their nutritional and added value properties. New aspects concerning the use of these wastes as by-products in food production additives or supplements with high nutritional and medicinal value have gained substantial interest, due to their possession of economically high-value products. In this sense, the present study describes a thorough characterization of phytochemical compounds from hydro-methanolic extract of broad beans testa by using ultra-high performance liquid chromatography (UHPLC) hyphenated with quadrupole-time-of-flight tandem mass spectrometry (QTOF-MS). The proposed analytical technique provides tentative characterization of 134 phenolic and other phytochemical compounds in the Vicia faba extract, most of which have not been described so far in broad beans. Thus, >85 phytochemicals (Amino acids, phenolic acids, flavonoids, lignans, and terpenoids derivatives) are being reported herein in broad beans pods for the first time. The characterization process was carried out using MS and MS2 data provided by the ESI-qTOF–MS, along with the use of the relevant literature based on the same botanical family. The data obtained demonstrates that the agro-industrial by-product could potentially be utilized as a promising source of bioactive ingredients to design new functional foods and nutraceuticals with a valuable future market. Furthermore, the obtained data may form a basis for future quantitative and bioavailability studies, which will be the next step in this present work.
    Keywords additives ; agroindustrial byproducts ; amino acids ; bioavailability ; faba beans ; flavonoids ; food production ; functional foods ; high-value products ; ingredients ; lignans ; metabolites ; phenolic acids ; phytopharmaceuticals ; pods ; tandem mass spectrometry ; terpenoids ; testa ; ultra-performance liquid chromatography ; Vicia faba ; wastes
    Language English
    Dates of publication 2017-03
    Size p. 87-96.
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 1111695-x
    ISSN 1873-7145 ; 0963-9969
    ISSN (online) 1873-7145
    ISSN 0963-9969
    DOI 10.1016/j.foodres.2017.01.014
    Database NAL-Catalogue (AGRICOLA)

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