LIVIVO - Search results -

Search results

Result 1 - 10 of total 35

Search options

Article ; Online: RNA Polymerase II Transcription in Pneumocystis

Diego A. Rojas / Fabiola Urbina / Aldo Solari / Edio Maldonado

International Journal of Molecular Sciences, Vol 23, Iss 6865, p

TFIIB from Pneumocystis carinii Can Replace the Transcriptional Functions of Fission Yeast Schizosaccharomyces pombe TFIIB In Vivo and In Vitro

2022 Volume 6865

Abstract: The Pneumocystis genus is an opportunistic fungal pathogen that infects patients with AIDS and immunocompromised individuals. The study of this fungus has been hampered due to the inability to grow it in a (defined media/pure) culture. However, the use ... ...

Abstract	The Pneumocystis genus is an opportunistic fungal pathogen that infects patients with AIDS and immunocompromised individuals. The study of this fungus has been hampered due to the inability to grow it in a (defined media/pure) culture. However, the use of modern molecular techniques and genomic analysis has helped researchers to understand its complex cell biology. The transcriptional process in the Pneumocystis genus has not been studied yet, although it is assumed that it has conventional transcriptional machinery. In this work, we have characterized the function of the RNA polymerase II (RNAPII) general transcription factor TFIIB from Pneumocystis carinii using the phylogenetically related biological model Schizosaccharomyces pombe . The results of this work show that Pneumocystis carinii TFIIB is able to replace the essential function of S. pombe TFIIB both in in vivo and in vitro assays. The S. pombe strain harboring the P carinii TFIIB grew slower than the parental wild-type S. pombe strain in complete media and in minimal media. The S. pombe cells carrying out the P. carinii TFIIB are larger than the wild-type cells, indicating that the TFIIB gene replacement confers a phenotype, most likely due to defects in transcription. P. carinii TFIIB forms very weak complexes with S. pombe TATA-binding protein on a TATA box promoter but it is able to form stable complexes in vitro when S. pombe TFIIF/RNAPII are added. P. carinii TFIIB can also replace the transcriptional function of S. pombe TFIIB in an in vitro assay. The transcription start sites (TSS) of the endogenous adh gene do not change when P. carinii TFIIB replaces S. pombe TFIIB, and neither does the TSS of the nmt1 gene, although this last gene is poorly transcribed in vivo in the presence of P. carinii TFIIB. Since transcription by RNA polymerase II in Pneumocystis is poorly understood, the results described in this study are promising and indicate that TFIIB from P. carinii can replace the transcriptional functions of S. pombe TFIIB, although the ...
Keywords	Pneumocystis carinii ; transcription ; general transcription factors (GTFs) ; RNA polymerase II ; Schizosaccharomyces pombe ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2022-06-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Vaccine Design against Chagas Disease Focused on the Use of Nucleic Acids

Edio Maldonado / Sebastian Morales-Pison / Fabiola Urbina / Aldo Solari

Vaccines, Vol 10, Iss 587, p

2022 Volume 587

Abstract: Chagas disease is caused by the protozoan Trypanosoma cruzi and is endemic to Central and South America. However, it has spread around the world and affects several million people. Treatment with currently available drugs cause several side effects and ... ...

Abstract	Chagas disease is caused by the protozoan Trypanosoma cruzi and is endemic to Central and South America. However, it has spread around the world and affects several million people. Treatment with currently available drugs cause several side effects and require long treatment times to eliminate the parasite, however, this does not improve the chronic effects of the disease such as cardiomyopathy. A therapeutic vaccine for Chagas disease may be able to prevent the disease and improve the chronic effects such as cardiomyopathy. This vaccine would be beneficial for both infected people and those which are at risk in endemic and non-endemic areas. In this article, we will review the surface antigens of T. cruzi , in order to choose those that are most antigenic and least variable, to design effective vaccines against the etiological agent of Chagas disease. Also, we discuss aspects of the design of nucleic acid-based vaccines, which have been developed and proven to be effective against the SARS-CoV-2 virus. The role of co-adjuvants and delivery carriers is also discussed. We present an example of a chimeric trivalent vaccine, based on experimental work, which can be used to design a vaccine against Chagas disease.
Keywords	DNA vaccine ; RNA vaccine ; vaccine delivery ; immune responses ; pandemic ; Medicine ; R
Subject code	610
Language	English
Publishing date	2022-04-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Natural Trypanosoma cruzi Infection and Climatic Season Influence the Developmental Capacity in Field-Caught Mepraia spinolai Nymphs

Juan Botzotz / Gabriel Méndez-Valdés / Sylvia Ortiz / Angélica López / Carezza Botto-Mahan / Aldo Solari

Insects, Vol 14, Iss 272, p

2023 Volume 272

Abstract: In this study, we evaluated the effect of the climatic season and infection by Trypanosoma cruzi , etiological agent of Chagas disease, on the molting capacity of the triatomine vector Mepraia spinolai endemic to Chile. We used wild-caught first-to- ... ...

Abstract	In this study, we evaluated the effect of the climatic season and infection by Trypanosoma cruzi , etiological agent of Chagas disease, on the molting capacity of the triatomine vector Mepraia spinolai endemic to Chile. We used wild-caught first-to-fourth instar nymphs during cooling (fall and winter) and warming (spring) periods. After capturing, nymphs were fed at the laboratory, and maintained under optimal rearing conditions. Feeding was repeated 40 days later. We followed-up the molting events on 709 nymphs, recording one, two or the absence of molts after two feeding opportunities. Within the same climatic period, only infected second- and fourth-instar nymphs from the warming period showed a larger proportion of double molting compared to uninfected nymphs. Regarding the climatic period, infected and uninfected first- and fourth-instar nymphs exhibited a larger proportion of double molting in the warming and cooling periods, respectively. The pattern of non-molting nymph occurrence suggests they probably reach diapause by environmental stochasticity. The effect of the climatic period and T. cruzi infection on the development of M. spinolai is an instar-dependent phenomenon, highlighting the occurrence of finely synchronized processes at different moments of the life cycle of such an hemimetabolous insect as triatomines.
Keywords	Mepraia spinolai ; Trypanosoma cruzi ; instar-dependent molting ; sylvatic kissing bug ; Chagas disease vector ; molting efficiency ; Science ; Q
Language	English
Publishing date	2023-03-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Seeing inferences

Ana Martín-Salguero / Carlo Reverberi / Aldo Solari / Luca Filippin / Christophe Pallier / Luca L. Bonatti

Scientific Reports, Vol 13, Iss 1, Pp 1-

brain dynamics and oculomotor signatures of non-verbal deduction

2023 Volume 11

Abstract: Abstract We often express our thoughts through words, but thinking goes well beyond language. Here we focus on an elementary but basic thinking process, disjunction elimination, elicited by elementary visual scenes deprived of linguistic content, ... ...

Abstract	Abstract We often express our thoughts through words, but thinking goes well beyond language. Here we focus on an elementary but basic thinking process, disjunction elimination, elicited by elementary visual scenes deprived of linguistic content, describing its neural and oculomotor correlates. We track two main components of a nonverbal deductive process: the construction of a logical representation (A or B), and its simplification by deduction (not A, therefore B). We identify the network active in the two phases and show that in the latter, but not in the former, it overlaps with areas known to respond to verbal logical reasoning. Oculomotor markers consistently differentiate logical processing induced by the construction of a representation, its simplification by deductive inference, and its maintenance when inferences cannot be drawn. Our results reveal how integrative logical processes incorporate novel experience in the flow of thoughts induced by visual scenes.
Keywords	Medicine ; R ; Science ; Q
Subject code	160
Language	English
Publishing date	2023-02-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: PAST AND PRESENT OF CHAGAS DISEASE IN NORTHERN CHILE PASADO Y PRESENTE DE LA ENFERMEDAD DE CHAGAS EN EL NORTE DE CHILE

Aldo Solari

Chungará (Arica) - Revista de Antropología Chilena, Vol 43, Iss 2, Pp 315-

2011 Volume 322

Abstract: Contemporary understandings of the epidemiology of Chagas disease as well as it’s clinical features, together with knowledge of the insect vectors and genotypes of the causal agent Trypanosoma cruzi are important pieces of information for comparisons ... ...

Abstract	Contemporary understandings of the epidemiology of Chagas disease as well as it’s clinical features, together with knowledge of the insect vectors and genotypes of the causal agent Trypanosoma cruzi are important pieces of information for comparisons with cases in the past. Evidence indicates that 4,000 years ago in northern Chile cases displaying digestive symptoms or ‘megacolon’ were frequent. Recent information on the dispersion of the domestic insect vector Triatoma infestans allows us to conclude that in the past only the wild Mepraia gajardoi insect vector transmitted Trypanosoma cruzi in its native area. This information together with current knowledge about Trypanosoma cruzi circulating in the area and transmitted by Mepraia gajardoi will be important to establish possible host-parasite association with Chagas disease pathology. El conocimiento de la epidemiología de la enfermedad de Chagas en el norte de Chile en nuestros días tales como las características clínicas, los insectos vectores del parásito hasta los genotipos del agente causal Trypanosoma cruzi son importantes para contrastar la situación con el pasado. La evidencia nos indica que hace 4.000 años en esta zona endémica hubo casos clínicos frecuentes con síntomas digestivos de la enfermedad de Chagas o megacolon. Los datos recientes sobre dispersión del insecto vector doméstico Triatoma infestans permiten concluir que en el pasado sólo el vector nativo y silvestre Mepraia gajardoi transmitía Trypanosoma cruzi. Esa información y el conocimiento actual de los genotipos de T. cruzi circulando en el área y transmitidos por M. gajardoi serán importantes para establecer posibles asociaciones huésped-parásito con la patología de la enfermedad de Chagas.
Keywords	Chagas ; genotipos T. cruzi ; insectos vectores ; T. cruzi genotypes ; insect vectors ; Anthropology ; GN1-890 ; Geography. Anthropology. Recreation ; G ; DOAJ:Anthropology ; DOAJ:Social Sciences
Language	English
Publishing date	2011-12-01T00:00:00Z
Publisher	Universidad de Tarapacá Facultad de Ciencias Sociales y Jurídicas
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Algunas reflexiones sobre el problema de los valores, la objetividad y el compromiso en las ciencias sociales

Aldo Solari

Revista Colombiana de Sociología, Vol 34, Iss 2, Pp 181-

2011 Volume 199

Keywords	Sociology (General) ; HM401-1281 ; Social Sciences ; H
Language	Spanish
Publishing date	2011-10-01T00:00:00Z
Publisher	Universidad Nacional de Colombia
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: T. cruzi DNA polymerase beta (Tcpolβ) is phosphorylated in vitro by CK1, CK2 and TcAUK1 leading to the potentiation of its DNA synthesis activity.

Edio Maldonado / Diego A Rojas / Fabiola Urbina / Aldo Solari

PLoS Neglected Tropical Diseases, Vol 15, Iss 7, p e

2021 Volume 0009588

Abstract: The unicellular protozoan Trypanosoma cruzi is the causing agent of Chagas disease which affects several millions of people around the world. The components of the cell signaling pathways in this parasite have not been well studied yet, although its ... ...

Abstract	The unicellular protozoan Trypanosoma cruzi is the causing agent of Chagas disease which affects several millions of people around the world. The components of the cell signaling pathways in this parasite have not been well studied yet, although its genome can encode several components able to transduce the signals, such as protein kinases and phosphatases. In a previous work we have found that DNA polymerase β (Tcpolβ) can be phosphorylated in vivo and this modification activates the synthesis activity of the enzyme. Tcpolβ is kinetoplast-located and is a key enzyme in the DNA base excision repair (BER) system. The polypeptide possesses several consensus phosphorylation sites for several protein kinases, however, a direct phosphorylation of those sites by specific kinases has not been reported yet. Tcpolβ has consensus phosphorylation sites for casein kinase 1 (CK1), casein kinase 2 (CK2) and aurora kinase (AUK). Genes encoding orthologues of those kinases exist in T. cruzi and we were able to identify the genes and to express them to investigate whether or no Tcpolβ could be a substrate for in vitro phosphorylation by those kinases. Both CK1 and TcAUK1 have auto-phosphorylation activities and they are able to phosphorylate Tcpolβ. CK2 cannot perform auto-phosphorylation of its subunits, however, it was able to phosphorylate Tcpolβ. Pharmacological inhibitors used to inhibit the homologous mammalian kinases can also inhibit the activity of T. cruzi kinases, although, at higher concentrations. The phosphorylation events carried out by those kinases can potentiate the DNA polymerase activity of Tcpolβ and it is discussed the role of the phosphorylation on the DNA polymerase and lyase activities of Tcpolβ. Taken altogether, indicates that CK1, CK2 and TcAUK1 can play an in vivo role regulating the function of Tcpolβ.
Keywords	Arctic medicine. Tropical medicine ; RC955-962 ; Public aspects of medicine ; RA1-1270
Subject code	572
Language	English
Publishing date	2021-07-01T00:00:00Z
Publisher	Public Library of Science (PLoS)
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: The Product of the Fission Yeast fhl1 Gene Binds to the HomolE Box and Activates In Vitro Transcription of Ribosomal Protein Genes

Edio Maldonado / Sebastian Morales-Pison / Fabiola Urbina / Claudia Arias / Christian Castillo / Lilian Jara / Aldo Solari

International Journal of Molecular Sciences, Vol 24, Iss 9472, p

2023 Volume 9472

Abstract: Fission yeast ribosomal protein genes (RPGs) contain a HomolD box as a core promoter element required for transcription. Some of the RPGs also contain a consensus sequence named HomolE, located upstream of the HomolD box. The HomolE box acts as an ... ...

Abstract	Fission yeast ribosomal protein genes (RPGs) contain a HomolD box as a core promoter element required for transcription. Some of the RPGs also contain a consensus sequence named HomolE, located upstream of the HomolD box. The HomolE box acts as an upstream activating sequence (UAS), and it is able to activate transcription in RPG promoters containing a HomolD box. In this work, we identified a HomolE-binding protein (HEBP) as a polypeptide of 100 kDa, which was able to bind to the HomolE box in a Southwestern blot assay. The features of this polypeptide were similar to the product of the fhl1 gene of fission yeast. The Fhl1 protein is the homolog of the FHL1 protein of budding yeast and possesses fork-head-associated (FHA) and fork-head (FH) domains. The product of the fhl1 gene was expressed and purified from bacteria, and it was demonstrated that is able to bind the HomolE box in an electrophoretic mobility assay (EMSA), as well as being able to activate in vitro transcription from an RPG gene promoter containing HomolE boxes upstream of the HomolD box. These results indicate that the product of the fhl1 gene of fission yeast can bind to the HomolE box, and it activates the transcription of RPGs.
Keywords	HomolE box ; Fhl1 protein ; HomolD box ; RPGs transcription ; Biology (General) ; QH301-705.5 ; Chemistry ; QD1-999
Subject code	570
Language	English
Publishing date	2023-05-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Experimental evidence of effective human–AI collaboration in medical decision-making

Carlo Reverberi / Tommaso Rigon / Aldo Solari / Cesare Hassan / Paolo Cherubini / GI Genius CADx Study Group / Andrea Cherubini

Scientific Reports, Vol 12, Iss 1, Pp 1-

2022 Volume 10

Abstract: Abstract Artificial Intelligence (ai) systems are precious support for decision-making, with many applications also in the medical domain. The interaction between mds and ai enjoys a renewed interest following the increased possibilities of deep learning ...

Abstract	Abstract Artificial Intelligence (ai) systems are precious support for decision-making, with many applications also in the medical domain. The interaction between mds and ai enjoys a renewed interest following the increased possibilities of deep learning devices. However, we still have limited evidence-based knowledge of the context, design, and psychological mechanisms that craft an optimal human–ai collaboration. In this multicentric study, 21 endoscopists reviewed 504 videos of lesions prospectively acquired from real colonoscopies. They were asked to provide an optical diagnosis with and without the assistance of an ai support system. Endoscopists were influenced by ai ( $$\textsc {or}=3.05$$ O R = 3.05 ), but not erratically: they followed the ai advice more when it was correct ( $$\textsc {or}=3.48$$ O R = 3.48 ) than incorrect ( $$\textsc {or}=1.85$$ O R = 1.85 ). Endoscopists achieved this outcome through a weighted integration of their and the ai opinions, considering the case-by-case estimations of the two reliabilities. This Bayesian-like rational behavior allowed the human–ai hybrid team to outperform both agents taken alone. We discuss the features of the human–ai interaction that determined this favorable outcome.
Keywords	Medicine ; R ; Science ; Q
Subject code	006
Language	English
Publishing date	2022-09-01T00:00:00Z
Publisher	Nature Portfolio
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

Article ; Online: Trypanosomatid Infections among Vertebrates of Chile

Juana P. Correa / Antonella Bacigalupo / Esteban Yefi-Quinteros / Gemma Rojo / Aldo Solari / Pedro E. Cattan / Carezza Botto-Mahan

Pathogens, Vol 9, Iss 661, p

A Systematic Review

2020 Volume 661

Abstract: We present a review on the natural infection by trypanosomatids of nonhuman vertebrates in Chile, aiming to synthesize and update the knowledge on the diversity of trypanosomatids infecting native and alien vertebrate species. To this end, we conducted a ...

Abstract	We present a review on the natural infection by trypanosomatids of nonhuman vertebrates in Chile, aiming to synthesize and update the knowledge on the diversity of trypanosomatids infecting native and alien vertebrate species. To this end, we conducted a systematic review of literature records published from 1900 to April 2020 on four databases, focusing on the 21 genera of trypanosomatids and Chile. The methods and findings of our review have been based on the preferred reporting items for systematic reviews and meta-analysis (prisma) checklist. We found 29,756 records but only 71 presented relevant information for this review. Overall, there are only two reported trypanosomatid genera infecting vertebrate species in Chile, the genera Trypanosoma and Leishmania . The former is mostly represented by Trypanosoma cruzi (90% of the total records) and to a much lesser extent by Trypanosoma avium , Trypanosoma humboldti , Trypanosoma lewisi , and a couple of unidentified trypanosomatids. A total of 25 mammals have been reported as being infected by T. cruzi , including 14 native and 11 alien species from Orders Artiodactyla, Carnivora, Chiroptera, Didelphimorphia, Lagomorpha, Perissodactyla, and Rodentia. Extensive screening studies using new analytical tools are necessary to grasp the whole potential diversity of trypanosomatid species infecting vertebrates in Chile.
Keywords	native mammals ; exotic mammals ; domestic mammals ; Trypanosoma cruzi ; hosts ; reservoirs ; Medicine ; R
Subject code	590
Language	English
Publishing date	2020-08-01T00:00:00Z
Publisher	MDPI AG
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

Full text online

Full text

Inter-library loan at ZB MED

Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

To top

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED

Full text online

More links

Kategorien

Inter-library loan at ZB MED