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Article ; Online: The microtubule cytoskeleton of radial glial progenitor cells.

Wimmer, Ryszard / Baffet, Alexandre D

2023 Volume 80, Page(s) 102709

Abstract: A high number of genetic mutations associated with cortical malformations are found in genes coding for microtubule-related factors. This has stimulated research to understand how the various microtubule-based processes are regulated to build a ... ...

Abstract	A high number of genetic mutations associated with cortical malformations are found in genes coding for microtubule-related factors. This has stimulated research to understand how the various microtubule-based processes are regulated to build a functional cerebral cortex. Here, we focus our review on the radial glial progenitor cells, the stem cells of the developing neocortex, summarizing research mostly performed in rodents and humans. We highlight how the centrosomal and acentrosomal microtubule networks are organized during interphase to support polarized transport and proper attachment of the apical and basal processes. We describe the molecular mechanism for interkinetic nuclear migration (INM), a microtubule-dependent oscillation of the nucleus. Finally, we describe how the mitotic spindle is built to ensure proper chromosome segregation, with a strong focus on factors mutated in microcephaly.
MeSH term(s)	Humans ; Microtubules ; Spindle Apparatus ; Stem Cells ; Neocortex ; Cell Nucleus
Language	English
Publishing date	2023-03-30
Publishing country	England
Document type	Journal Article ; Review ; Research Support, Non-U.S. Gov't
ZDB-ID	1078046-4
ISSN	1873-6882 ; 0959-4388
ISSN (online)	1873-6882
ISSN	0959-4388
DOI	10.1016/j.conb.2023.102709
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Article ; Online: Evaluation of miR-155 silencing using a molecular beacon in human lung adenocarcinoma cell line.

Alexandre, Daniela / Fernandes, Alexandra R / Baptista, Pedro V / Cruz, Carla

Talanta

2024 Volume 274, Page(s) 126052

Abstract: Lung cancer (LC) is a leading cause of global cancer-related deaths, highlighting the development of innovative methods for biomarker detection improving the early diagnostics. microRNAs (miRs) alterations are known to be involved in the initiation and ... ...

Abstract	Lung cancer (LC) is a leading cause of global cancer-related deaths, highlighting the development of innovative methods for biomarker detection improving the early diagnostics. microRNAs (miRs) alterations are known to be involved in the initiation and progression of human cancers and can act as biomarkers for diagnostics and treatment. Herein, we develop the application of molecular beacon (MB) technology to monitor miR-155-3p expression in human lung adenocarcinoma A549 cells without complementary DNA synthesis, amplification, or expensive reagents. Furthermore, we produced gold nanoparticles (AuNPs) for delivering antisense oligonucleotides into A549 cells to reduce miR-155-3p expression, which was subsequently detectable using the MB. The MB was designed and structural characterized by Förster Resonance Energy Transfer (FRET)-melting, Circular Dichroism (CD), Nuclear magnetic resonance (NMR), and fluorometric experiments, and then the hybridization conditions were optimized for an in vitro approach involving the detection of miR-155-3p in total RNA extracted from A549 cell line. The expression profile of miR-155-3p was obtained by RT-qPCR. The results demonstrated that MB was properly designed and showed efficacy in targeting miR-155-3p. Furthermore, a limit of detection down to nanomolar concentration was achieved and the specificity of the biosensor was proved. Moreover, the self-assembly of ASOs with AuNPs exhibited exceptional target specificity, effectively silencing miR-155-3p. Notably, compared to lipid-based transfection agent, AuNPs displayed superior silencing efficiency. We highlighted the ability of MB to detect changes in the target gene expression after gene silencing. Overall, this innovative approach represents a promising tool for detecting various biomarkers at the same time, with potential applications in clinical settings.
Language	English
Publishing date	2024-04-07
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	1500969-5
ISSN	1873-3573 ; 0039-9140
ISSN (online)	1873-3573
ISSN	0039-9140
DOI	10.1016/j.talanta.2024.126052
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Article ; Online: The potential of using data planning and analysis strategies to transform primary healthcare.

Chiavegato Filho, Alexandre D P / Cupertino de Barros, Fernando P

Revista de saude publica

2024 Volume 57Suppl 3, Issue Suppl 3, Page(s) 1s

MeSH term(s)	Humans ; Brazil ; Primary Health Care
Language	Portuguese
Publishing date	2024-04-15
Publishing country	Brazil
Document type	Editorial
ZDB-ID	732179-x
ISSN	1518-8787 ; 0034-8910
ISSN (online)	1518-8787
ISSN	0034-8910
DOI	10.11606/s1518-8787.2023057supl3ed
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Article: Mechanisms of Acquired Resistance and Tolerance to EGFR Targeted Therapy in Non-Small Cell Lung Cancer.

Chhouri, Houssein / Alexandre, David / Grumolato, Luca

Cancers

2023 Volume 15, Issue 2

Abstract: Non-small cell lung cancers (NSCLC) harboring activating mutations of the epidermal growth factor receptor (EGFR) are treated with specific tyrosine kinase inhibitors (EGFR-TKIs) of this receptor, resulting in clinically responses that can generally last ...

Abstract	Non-small cell lung cancers (NSCLC) harboring activating mutations of the epidermal growth factor receptor (EGFR) are treated with specific tyrosine kinase inhibitors (EGFR-TKIs) of this receptor, resulting in clinically responses that can generally last several months. Unfortunately, EGFR-targeted therapy also favors the emergence of drug tolerant or resistant cells, ultimately resulting in tumor relapse. Recently, cellular barcoding strategies have arisen as a powerful tool to investigate the clonal evolution of these subpopulations in response to anti-cancer drugs. In this review, we provide an overview of the currently available treatment options for NSCLC, focusing on EGFR targeted therapy, and discuss the common mechanisms of resistance to EGFR-TKIs. We also review the characteristics of drug-tolerant persister (DTP) cells and the mechanistic basis of drug tolerance in EGFR-mutant NSCLC. Lastly, we address how cellular barcoding can be applied to investigate the response and the behavior of DTP cells upon EGFR-TKI treatment.
Language	English
Publishing date	2023-01-13
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2527080-1
ISSN	2072-6694
ISSN	2072-6694
DOI	10.3390/cancers15020504
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Article ; Online: Diagnosing and treating pericarditis and myocarditis in the emergency department.

McGuire, Morgan / Harvey, Warren / Brady, Tucker / Nguyen, Alexandre D

Emergency medicine practice

2023 Volume 25, Issue 7, Page(s) 1–24

Abstract: Pericarditis and myocarditis represent a challenging set of diseases to diagnose and treat. These diseases typically present with chest pain and dyspnea in previously healthy young people, often in the weeks following a viral illness, including COVID-19. ...

Abstract	Pericarditis and myocarditis represent a challenging set of diseases to diagnose and treat. These diseases typically present with chest pain and dyspnea in previously healthy young people, often in the weeks following a viral illness, including COVID-19. Nonetheless, the etiologies can be very diverse, including infectious, noninfectious, drug-induced, and autoimmune causes. This review focuses on the evaluation, diagnosis, and management of emergency department patients presenting with pericarditis and myocarditis and summarizes current guidelines and best-practice medical management strategies in order to avoid potential life-threatening cardiac complications.
MeSH term(s)	Humans ; Adolescent ; Myocarditis/diagnosis ; Myocarditis/therapy ; Myocarditis/etiology ; COVID-19/therapy ; Pericarditis/diagnosis ; Pericarditis/therapy ; Pericarditis/etiology ; Emergency Service, Hospital ; Diagnosis, Differential ; COVID-19 Testing
Language	English
Publishing date	2023-07-01
Publishing country	United States
Document type	Review ; Journal Article
ISSN	1559-3908
ISSN (online)	1559-3908
Database	MEDical Literature Analysis and Retrieval System OnLINE

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Article ; Online: Fast and pure phase-shifting off-axis holographic microscopy with a digital micromirror device.

Brodoline, Alexey / Alexandre, Daniel / Gross, Michel

Applied optics

2022 Volume 61, Issue 15, Page(s) 4296–4302

Abstract: We present a phase-shifting digital holographic microscopy technique, where a digital micromirror device enables to perform a precise phase-only shift of the reference wave. By coupling the beam into a monomode fiber, we obtain a laser mode with a ... ...

Abstract	We present a phase-shifting digital holographic microscopy technique, where a digital micromirror device enables to perform a precise phase-only shift of the reference wave. By coupling the beam into a monomode fiber, we obtain a laser mode with a constant phase shift, equally acting on all pixels of the hologram. This method has the advantage of being relatively simple and compatible with high frame rate cameras, which makes it of great interest for the observation of fast phenomena. We demonstrate the validity of the technique in an off-axis configuration by imaging living paramecia caudata.
Language	English
Publishing date	2022-10-03
Publishing country	United States
Document type	Journal Article
ISSN	1539-4522
ISSN (online)	1539-4522
DOI	10.1364/AO.452382
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Article ; Online: Premature termination of transcription is shaped by Rho and translated uORFS in Mycobacterium tuberculosis

Alexandre D’Halluin / Peter Polgar / Terry Kipkorir / Zaynah Patel / Teresa Cortes / Kristine B. Arnvig

iScience, Vol 26, Iss 4, Pp 106465- (2023)

2023

Abstract: Summary: Little is known about the decisions behind transcription elongation versus termination in the human pathogen Mycobacterium tuberculosis (M.TB). By applying Term-seq to M.TB we found that the majority of transcription termination is premature and ...

Abstract	Summary: Little is known about the decisions behind transcription elongation versus termination in the human pathogen Mycobacterium tuberculosis (M.TB). By applying Term-seq to M.TB we found that the majority of transcription termination is premature and associated with translated regions, i.e., within previously annotated or newly identified open reading frames. Computational predictions and Term-seq analysis, upon depletion of termination factor Rho, suggests that Rho-dependent transcription termination dominates all transcription termination sites (TTS), including those associated with regulatory 5′ leaders. Moreover, our results suggest that tightly coupled translation, in the form of overlapping stop and start codons, may suppress Rho-dependent termination. This study provides detailed insights into novel M.TB cis-regulatory elements, where Rho-dependent, conditional termination of transcription and translational coupling together play major roles in gene expression control. Our findings contribute to a deeper understanding of the fundamental regulatory mechanisms that enable M.TB adaptation to the host environment offering novel potential points of intervention.
Keywords	Biological sciences ; Molecular biology ; Molecular mechanism of gene regulation ; Science ; Q
Subject code	612
Language	English
Publishing date	2023-04-01T00:00:00Z
Publisher	Elsevier
Document type	Article ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Book ; Online: Hierarchical B-frame Video Coding Using Two-Layer CANF without Motion Coding

Alexandre, David / Hang, Hsueh-Ming / Peng, Wen-Hsiao

2023

Abstract: Typical video compression systems consist of two main modules: motion coding and residual coding. This general architecture is adopted by classical coding schemes (such as international standards H.265 and H.266) and deep learning-based coding schemes. ... ...

Abstract	Typical video compression systems consist of two main modules: motion coding and residual coding. This general architecture is adopted by classical coding schemes (such as international standards H.265 and H.266) and deep learning-based coding schemes. We propose a novel B-frame coding architecture based on two-layer Conditional Augmented Normalization Flows (CANF). It has the striking feature of not transmitting any motion information. Our proposed idea of video compression without motion coding offers a new direction for learned video coding. Our base layer is a low-resolution image compressor that replaces the full-resolution motion compressor. The low-resolution coded image is merged with the warped high-resolution images to generate a high-quality image as a conditioning signal for the enhancement-layer image coding in full resolution. One advantage of this architecture is significantly reduced computational complexity due to eliminating the motion information compressor. In addition, we adopt a skip-mode coding technique to reduce the transmitted latent samples. The rate-distortion performance of our scheme is slightly lower than that of the state-of-the-art learned B-frame coding scheme, B-CANF, but outperforms other learned B-frame coding schemes. However, compared to B-CANF, our scheme saves 45% of multiply-accumulate operations (MACs) for encoding and 27% of MACs for decoding. The code is available at https://nycu-clab.github.io.
Keywords	Computer Science - Computer Vision and Pattern Recognition ; Electrical Engineering and Systems Science - Image and Video Processing
Subject code	003
Publishing date	2023-04-05
Publishing country	us
Document type	Book ; Online
Database	BASE - Bielefeld Academic Search Engine (life sciences selection)

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Article: Prescription and Effects of Cardiorespiratory Training in Individuals with Intellectual Disability: A Systematic Review.

Jacinto, Miguel / Oliveira, Rafael / Martins, Alexandre D / Brito, João Paulo / Matos, Rui / Ferreira, José Pedro

Healthcare (Basel, Switzerland)

2023 Volume 11, Issue 14

Abstract: This study aims to systematize effects of cardiorespiratory training (CT) programs in individuals with intellectual disability (ID) and identifying the fundamental and structuring aspects for the prescription of CT. This systematic review was carried out ...

Abstract	This study aims to systematize effects of cardiorespiratory training (CT) programs in individuals with intellectual disability (ID) and identifying the fundamental and structuring aspects for the prescription of CT. This systematic review was carried out through four databases (Pubmed, Web of Science, Scopus, and SPORTDiscus), considering data from the period between 2013 and 2022. From 257 studies, 12 studies were included in this systematic review. Three studies used interval CT, while seven used continuous CT. Seven were carried out in the population with Down syndrome, while only three were carried out with participants with ID. The CT programs had the following characteristics: duration of 8 to 12 weeks, weekly frequency of three sessions, for 20 to 60 min, the intensity of 50% to 80% of maximal heart rate or 70% to 80% of peak oxygen consumption, using an ergometer cycle or an outdoor walking. The studies reported improvements in cardiorespiratory function, lipid, hemodynamic and metabolic profile, body composition, and neuromuscular and cognitive capacity. This review presents characteristics and recommendations that technicians can follow when structuring, prescribing, and implementing CT programs to individuals with ID.
Language	English
Publishing date	2023-07-24
Publishing country	Switzerland
Document type	Journal Article ; Review
ZDB-ID	2721009-1
ISSN	2227-9032
ISSN	2227-9032
DOI	10.3390/healthcare11142106
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Article: The improvement of the functional properties of a chickpea protein isolate through proteolysis with three proteases

Goertzen, Alexandre D. / Nickerson, Michael T. / Tanaka, Takuji

Cereal chemistry. 2021 May, v. 98, no. 3

2021

Abstract: BACKGROUND AND OBJECTIVES: Enzymatic hydrolysis has previously been shown to have beneficial influences on the protein functionality of pulse protein isolates. To apply and optimize these benefits in chickpeas, a chickpea protein isolate (CPI) was ... ...

Abstract	BACKGROUND AND OBJECTIVES: Enzymatic hydrolysis has previously been shown to have beneficial influences on the protein functionality of pulse protein isolates. To apply and optimize these benefits in chickpeas, a chickpea protein isolate (CPI) was hydrolyzed with three different proteases (trypsin, pepsin, and papain), to three different degrees of hydrolysis (DH): 5%, 10%, and 15%. Changes to zeta potential, hydrophobicity, water and oil holding capacity (WHC and OHC), as well as solubility, emulsifying, and foaming properties at pH 4.0, 7.0, and 10.0 of the various hydrolysates were then quantified and compared with one another in order to determine optimal hydrolytic conditions. FINDINGS: Increasing length of enzymatic treatments led to a gradual increase in zeta potential, and a rise in protein hydrophobicity. The solubility of trypsin and papain hydrolyzed CPI proteins increased at pH 10.0, while minor decreases in solubility were noted at pH 7.0. Increasing length of pepsin and papain treatment was noted to improve the water holding capacity (WHC), whereas hydrolysis with all three enzymes led to decreases in oil holding capacity (OHC). The emulsifying activity index (EAI) of the CPI increased following hydrolysis with all enzymes at pH 4.0 and 10.0, while at pH 7.0 no changes were observed. The emulsifying stability index (ESI) of the CPI increased following trypsin treatment at pH 4.0; at pH 10.0 emulsions appeared stable, exhibiting no separation over a 30‐min period. Foaming capacity (FC) increased following enzyme treatments at pH 4.0, pH 7.0, and more dramatically at pH 10.0. Foaming stability (FS) worsened at pH 10.0 following higher levels of trypsin hydrolysis (DH > 10%). CONCLUSIONS: Approximately 15% hydrolysis with papain was shown as the best conditions to augment the emulsifying, foaming and water holding properties of a CPI material, potentially allowing for the generation of specialized chickpea protein ingredients. SIGNIFICANCE AND NOVELTY: A total of nine distinct CPI hydrolysates were generated and compared with one another to determine optimal hydrolytic conditions with respect to the functional properties of the CPI.
Keywords	chickpeas ; enzymatic hydrolysis ; hydrolysis ; hydrophobicity ; oils ; pH ; papain ; pepsin ; protein isolates ; proteolysis ; solubility ; trypsin ; zeta potential
Language	English
Dates of publication	2021-05
Size	p. 439-449.
Publishing place	John Wiley & Sons, Ltd
Document type	Article
Note	JOURNAL ARTICLE
ZDB-ID	242360-1
ISSN	0009-0352
ISSN	0009-0352
DOI	10.1002/cche.10383
Database	NAL-Catalogue (AGRICOLA)

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