Article ; Online: Venomous gland transcriptome and venom proteomic analysis of the scorpion Androctonus amoreuxi reveal new peptides with anti-SARS-CoV-2 activity.
2023 Volume 173, Page(s) 171139
Abstract: The recent COVID-19 pandemic shows the critical need for novel broad spectrum antiviral agents. Scorpion venoms are known to contain highly bioactive peptides, several of which have demonstrated strong antiviral activity against a range of viruses. We ... ...
| Abstract | The recent COVID-19 pandemic shows the critical need for novel broad spectrum antiviral agents. Scorpion venoms are known to contain highly bioactive peptides, several of which have demonstrated strong antiviral activity against a range of viruses. We have generated the first annotated reference transcriptome for the Androctonus amoreuxi venom gland and used high performance liquid chromatography, transcriptome mining, circular dichroism and mass spectrometric analysis to purify and characterize twelve previously undescribed venom peptides. Selected peptides were tested for binding to the receptor-binding domain (RBD) of the SARS-CoV-2 spike protein and inhibition of the spike RBD - human angiotensin-converting enzyme 2 (hACE2) interaction using surface plasmon resonance-based assays. Seven peptides showed dose-dependent inhibitory effects, albeit with IC |
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| MeSH term(s) | Animals ; Humans ; SARS-CoV-2/metabolism ; Scorpions/chemistry ; COVID-19 ; Transcriptome ; Proteomics ; Pandemics ; Peptides/metabolism ; Antiviral Agents/pharmacology ; Scorpion Venoms/chemistry ; Protein Binding ; Animals, Poisonous ; Spike Glycoprotein, Coronavirus | |||||
| Chemical Substances | spike protein, SARS-CoV-2 ; Peptides ; Antiviral Agents ; Scorpion Venoms ; Spike Glycoprotein, Coronavirus | |||||
| Language | English | |||||
| Publishing date | 2023-12-22 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 769028-9 | |||||
| ISSN | 1873-5169 ; 0196-9781 | |||||
| ISSN (online) | 1873-5169 | |||||
| ISSN | 0196-9781 | |||||
| DOI | 10.1016/j.peptides.2023.171139 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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