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  1. AU="Alisarli, Mustafa"
  2. AU="Kaufman, Dan"
  3. AU=Weigelin Bettina
  4. AU="Arun Kumar S"
  5. AU="Dahlsjö, Cecilia A L"
  6. AU="Gavigan, Patrick"
  7. AU="Liu, Ye"
  8. AU="Changyang Xing"
  9. AU="Sarovich, Derek S"
  10. AU="Joseph P. Zackular"
  11. AU="Maes, Evelyn"
  12. AU="Haixiang Zhou"
  13. AU="Choi, Jinyong"
  14. AU="González-Mansilla, Ana"
  15. AU="Cho Kilwon"
  16. AU="Rachide Onadja, Palamanga Abdoul"
  17. AU="Bertacca, Sofia"
  18. AU="Bansal, Ayush"
  19. AU="Liao, Naikai"
  20. AU="Gomes, Lux Attiê Santos"
  21. AU="İdil Su Canıtez"
  22. AU="Baptiste Duceau"
  23. AU="Pedro Seoane-Zonjic"
  24. AU="Guthridge, Carla J"
  25. AU=Meena Netra P.
  26. AU="Boustany, Tara"

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  1. Buch ; Dissertation / Habilitation: Vermehrung von Staphylococcus aureus und Enterotoxinbildung in türkischen Puddingspeisen

    Alisarli, Mustafa

    1997  

    Verfasserangabe von Mustafa Alisarli
    Schlagwörter Türkei ; Pudding ; Enterotoxin
    Sprache Deutsch
    Umfang 100 S. : graph. Darst.
    Erscheinungsland Schweiz
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Zürich, Univ., Diss., 1997
    Anmerkung Zsfassung in engl. Sprache
    Bemerkung Abt. Nussallee/Bereichsbibl. ZBMed: AY 18678
    HBZ-ID HT007907719
    Datenquelle Katalog ZB MED Ernährung, Umwelt, Agrar

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  2. Artikel: Serotyping and antibiotic resistance of Listeria monocytogenes isolated from raw water buffalo milk and milk products

    Terzi Gulel, Goknur / Gucukoglu, Ali / Cadirci, Ozgur / Saka, Erdem / Alisarli, Mustafa

    Journal of food science. 2020 Sept., v. 85, no. 9

    2020  

    Abstract: This study was aimed to investigate the presence of Listeria monocytogenes in raw water buffalo milk and milk products, besides determining its serotype and the extent of its resistance against various antibiotics. A total of 188 samples of raw water ... ...

    Abstract This study was aimed to investigate the presence of Listeria monocytogenes in raw water buffalo milk and milk products, besides determining its serotype and the extent of its resistance against various antibiotics. A total of 188 samples of raw water buffalo milk and milk products were collected from Samsun Province, Turkey between November 2012 and May 2013. The classical culture technique was used to isolate and identify L. monocytogenes, as described in EN ISO 11290‐1. The isolates were confirmed as L. monocytogenes by using PCR with (hylA) primers specific for the hemolysin gene. The antimicrobial susceptibility test was achieved by using the VITEK 2 compact system and VITEK 2 AST‐P640 card. L. monocytogenes was found in 7 (3.7%) of the 188 samples. Four of them were obtained from cheese and three from milk samples. Whereas, L. monocytogenes was not detected in any of the clotted cream samples. A total of 13 isolates were confirmed by PCR as L. monocytogenes. Among these isolates, one was 1/2c (or 3c) (7.6%), three were 4b (or 4d, 4e) (23%), four were 1/2b (or 3b) (30.7%), and the other five isolates were serotype 1/2a (or 3a) (38.4%). The highest antimicrobial resistance was recorded against fosfomycine (100%) followed by oxacillin (92%), penicillin (84%), and erythromycin (69%). However, no resistance was determined against ciprofloxacin, gentamicin, and tigecycline. PRACTICAL APPLICATION: This study showed that some samples of raw buffalo milk and the milk products were contaminated with Listeria monocytogenes. The serotype with the highest prevalence was determined as L. monocytogenes 1/2a. This study also demonstrated that most of the L. monocytogenes isolates had developed multiresistance to many frequently used medical antimicrobial agents.
    Schlagwörter Listeria monocytogenes ; antibiotic resistance ; buffalo milk ; buffaloes ; cheeses ; ciprofloxacin ; cream ; erythromycin ; food science ; genes ; gentamicin ; hemolysins ; milk ; oxacillin ; serotypes ; tigecycline
    Sprache Englisch
    Erscheinungsverlauf 2020-09
    Umfang p. 2889-2895.
    Erscheinungsort John Wiley & Sons, Ltd
    Dokumenttyp Artikel
    Anmerkung NAL-AP-2-clean ; JOURNAL ARTICLE
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/1750-3841.15376
    Datenquelle NAL Katalog (AGRICOLA)

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  3. Artikel ; Online: Serotyping and antibiotic resistance of Listeria monocytogenes isolated from raw water buffalo milk and milk products.

    Terzi Gulel, Goknur / Gucukoglu, Ali / Cadirci, Ozgur / Saka, Erdem / Alisarli, Mustafa

    Journal of food science

    2020  Band 85, Heft 9, Seite(n) 2889–2895

    Abstract: This study was aimed to investigate the presence of Listeria monocytogenes in raw water buffalo milk and milk products, besides determining its serotype and the extent of its resistance against various antibiotics. A total of 188 samples of raw water ... ...

    Abstract This study was aimed to investigate the presence of Listeria monocytogenes in raw water buffalo milk and milk products, besides determining its serotype and the extent of its resistance against various antibiotics. A total of 188 samples of raw water buffalo milk and milk products were collected from Samsun Province, Turkey between November 2012 and May 2013. The classical culture technique was used to isolate and identify L. monocytogenes, as described in EN ISO 11290-1. The isolates were confirmed as L. monocytogenes by using PCR with (hylA) primers specific for the hemolysin gene. The antimicrobial susceptibility test was achieved by using the VITEK 2 compact system and VITEK 2 AST-P640 card. L. monocytogenes was found in 7 (3.7%) of the 188 samples. Four of them were obtained from cheese and three from milk samples. Whereas, L. monocytogenes was not detected in any of the clotted cream samples. A total of 13 isolates were confirmed by PCR as L. monocytogenes. Among these isolates, one was 1/2c (or 3c) (7.6%), three were 4b (or 4d, 4e) (23%), four were 1/2b (or 3b) (30.7%), and the other five isolates were serotype 1/2a (or 3a) (38.4%). The highest antimicrobial resistance was recorded against fosfomycine (100%) followed by oxacillin (92%), penicillin (84%), and erythromycin (69%). However, no resistance was determined against ciprofloxacin, gentamicin, and tigecycline. PRACTICAL APPLICATION: This study showed that some samples of raw buffalo milk and the milk products were contaminated with Listeria monocytogenes. The serotype with the highest prevalence was determined as L. monocytogenes 1/2a. This study also demonstrated that most of the L. monocytogenes isolates had developed multiresistance to many frequently used medical antimicrobial agents.
    Mesh-Begriff(e) Animals ; Anti-Bacterial Agents/pharmacology ; Buffaloes ; Cheese/microbiology ; DNA Primers/genetics ; Drug Resistance, Bacterial ; Food Contamination/analysis ; Food Microbiology ; Listeria monocytogenes/classification ; Listeria monocytogenes/drug effects ; Listeria monocytogenes/genetics ; Listeria monocytogenes/isolation & purification ; Milk/microbiology ; Polymerase Chain Reaction ; Serotyping ; Turkey
    Chemische Substanzen Anti-Bacterial Agents ; DNA Primers
    Sprache Englisch
    Erscheinungsdatum 2020-08-14
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/1750-3841.15376
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  4. Artikel: Detection of C. Botulinum Types in Honey by mPCR

    Gücükoğlu, Ali / Terzi, Göknur / Çadirci, Özgür / Alişarli, Mustafa / Kevenk, Onur / Uyanik, Tolga

    Journal of food science. 2014 Apr., v. 79, no. 4

    2014  

    Abstract: The aim of this study was to determine the prevalence of Clostridium botulinum in honey samples using conventional methods and multiplex PCR (mPCR). A total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open ... ...

    Abstract The aim of this study was to determine the prevalence of Clostridium botulinum in honey samples using conventional methods and multiplex PCR (mPCR). A total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open bazaars, and supermarkets in Samsun, Turkey. Of 150 honey samples, 4 (2.6%) were positive for the botulinum neurotoxin gene by mPCR analysis. A total of 4 C. botulinum isolates were obtained from the mPCR positive samples, of which 3 were type A and 1 was type B. No samples were positive regarding the type E and type F neurotoxin genes. This is the first report of type A and type B spores of C. botulinum being detected and isolated in Turkey. This study revealed that some honey samples may present a potential hazard for food borne and infant botulism.
    Schlagwörter Clostridium botulinum ; apiaries ; botulism ; foods ; genes ; honey ; polymerase chain reaction ; spores ; supermarkets ; Turkey (country)
    Sprache Englisch
    Erscheinungsverlauf 2014-04
    Umfang p. M600-M603.
    Erscheinungsort The Institute
    Dokumenttyp Artikel
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/1750-3841.12402
    Datenquelle NAL Katalog (AGRICOLA)

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  5. Buch ; Dissertation / Habilitation: Vermehrung von Staphylococcus aureu und Enterotoxinbildung in türkischen Puddingspeisen

    Alisarli, Mustafa

    1997  

    Verfasserangabe vorgelegt von Mustafa Alisarli
    Sprache Deutsch
    Umfang 100 S, graph. Darst, 21 cm
    Verlag Zentralstelle der Studentenschaft
    Erscheinungsort Zürich
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Univ., Diss.--Zürich, 1997
    Datenquelle Bibliothek der Tierärztlichen Hochschule Hannover

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  6. Buch ; Dissertation / Habilitation: Vermehrung von Staphylococcus aureu und Enterotoxinbildung in türkischen Puddingspeisen

    Alisarli, Mustafa

    1997  

    Verfasserangabe vorgelegt von Mustafa Alisarli
    Sprache Deutsch
    Umfang 100 S, graph. Darst, 21 cm
    Verlag Zentralstelle der Studentenschaft
    Erscheinungsort Zürich
    Dokumenttyp Buch ; Dissertation / Habilitation
    Dissertation / Habilitation Univ., Diss.--Zürich, 1997
    Datenquelle Ehemaliges Sondersammelgebiet Küsten- und Hochseefischerei

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  7. Artikel ; Online: Detection of C. botulinum types in honey by mPCR.

    Gücükoğlu, Ali / Terzi, Göknur / Çadirci, Özgür / Alişarli, Mustafa / Kevenk, Onur / Uyanik, Tolga

    Journal of food science

    2014  Band 79, Heft 4, Seite(n) M600–3

    Abstract: The aim of this study was to determine the prevalence of Clostridium botulinum in honey samples using conventional methods and multiplex PCR (mPCR). A total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open ... ...

    Abstract The aim of this study was to determine the prevalence of Clostridium botulinum in honey samples using conventional methods and multiplex PCR (mPCR). A total number of 150 honey samples were randomly collected from apiaries, retail shops, weekly open bazaars, and supermarkets in Samsun, Turkey. Of 150 honey samples, 4 (2.6%) were positive for the botulinum neurotoxin gene by mPCR analysis. A total of 4 C. botulinum isolates were obtained from the mPCR positive samples, of which 3 were type A and 1 was type B. No samples were positive regarding the type E and type F neurotoxin genes. This is the first report of type A and type B spores of C. botulinum being detected and isolated in Turkey. This study revealed that some honey samples may present a potential hazard for food borne and infant botulism.
    Mesh-Begriff(e) Botulism/microbiology ; Clostridium botulinum/genetics ; Clostridium botulinum/isolation & purification ; Food Microbiology ; Genes, Bacterial ; Honey/microbiology ; Humans ; Infant ; Neurotoxins/genetics ; Polymerase Chain Reaction/methods ; Spores, Bacterial ; Turkey
    Chemische Substanzen Neurotoxins
    Sprache Englisch
    Erscheinungsdatum 2014-04
    Erscheinungsland United States
    Dokumenttyp Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/1750-3841.12402
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  8. Artikel ; Online: Determination of enterotoxigenic and methicillin resistant Staphylococcus aureus in ice cream.

    Gücükoğlu, Ali / Çadirci, Özgür / Terzi, Göknur / Kevenk, T Onur / Alişarli, Mustafa

    Journal of food science

    2013  Band 78, Heft 5, Seite(n) M738–41

    Abstract: The aim of this study was to determine the prevalence of enterotoxigenic and methicillin-resistant Staphylococcus aureus in ice creams. After culture-based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was ... ...

    Abstract The aim of this study was to determine the prevalence of enterotoxigenic and methicillin-resistant Staphylococcus aureus in ice creams. After culture-based identification of isolates, the presence of 16S rRNA and nuc was confirmed by mPCR. S. aureus was identified in 18 of 56 fruity (32.1%), 4 of 32 vanilla (12.5%), and 1 of 12 chocolate (8.3%) ice creams. S. aureus was identified as 38 isolates in 23 ice cream samples by culture-based techniques, but only 35 isolates were confirmed by PCR as S. aureus. To determine the enterotoxigenic properties of PCR-confirmed S. aureus isolates, a toxin detection kit was used (SET RPLA®). Of the 12 enterotoxigenic S. aureus isolates, 9 SEB (75%), 1 SED (8.3%), 1 SEB+SED (8.3%), and 1 SEA+SEB+SED (8.3%) expressing isolates were found. The presence of enterotoxin genes (sea, seb, sed) was identified in 13 (37.1%) out of 35 isolates by the mPCR technique. In the ice cream isolates, the sea, seb, and sed genes were detected: 1 sea (7.6%), 9 seb (69.2%), 1 sed (7.6%), 1 seb+sed (7.6%), and 1 sea+seb+sed (7.6%), respectively. The sec gene was not detected in any of these isolates. One of the 35 (2.8%) S. aureus strain was mecA positive.
    Mesh-Begriff(e) DNA, Bacterial/genetics ; Enterotoxins/isolation & purification ; Food Contamination/analysis ; Food Microbiology ; Ice Cream/microbiology ; Methicillin-Resistant Staphylococcus aureus/classification ; Methicillin-Resistant Staphylococcus aureus/genetics ; Methicillin-Resistant Staphylococcus aureus/isolation & purification ; Polymerase Chain Reaction/methods ; RNA, Ribosomal, 16S/genetics ; Staphylococcus aureus/classification ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification
    Chemische Substanzen DNA, Bacterial ; Enterotoxins ; RNA, Ribosomal, 16S ; enterotoxin B, staphylococcal (39424-53-8)
    Sprache Englisch
    Erscheinungsdatum 2013-05
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/1750-3841.12093
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  9. Artikel ; Online: Detection of enterotoxigenic Staphylococcus aureus in raw milk and dairy products by multiplex PCR.

    Gücükoğlu, Ali / Kevenk, T Onur / Uyanik, Tolga / Cadirci, Ozgür / Terzi, Göknur / Alişarli, Mustafa

    Journal of food science

    2012  Band 77, Heft 11, Seite(n) M620–3

    Abstract: The aim of this study was to investigate the prevalence of enterotoxigenic Staphylococcus aureus in 122 samples, including 60 raw milk, 32 white cheese, 10 kashar cheese, 10 butter, and 10 ice cream samples obtained from Samsun province, Turkey. In this ... ...

    Abstract The aim of this study was to investigate the prevalence of enterotoxigenic Staphylococcus aureus in 122 samples, including 60 raw milk, 32 white cheese, 10 kashar cheese, 10 butter, and 10 ice cream samples obtained from Samsun province, Turkey. In this study, S. aureus was detected in 64 samples, including raw milk (45/60; 75%), white cheese (12/32; 37.5%), kashar cheese (3/10; 30%), butter (3/10; 30%), and ice cream (1/10; 10%) samples. A total of 81 isolates were identified as S. aureus by PCR with the presence of 16S rRNA and nuc genes. The presence of genes encoding the staphylococcal enterotoxins (SEs) SEA, SEB, SEC, and SED was detected by multiplex PCR. According to the analysis, seven isolates from the raw milk samples (7/51; 13.7%) were enterotoxigenic; five of them produced SEA (5/7; 71.4%), one produced SEB (1/7; 14.2%), and one produced SEA+SEB (1/7; 14.2%). Four isolates from the white cheese samples (4/21; 19%) produced the SEA (1/4; 25%), SEC (1/4; 25%), SED (1/4; 25%), and SEA+SED (1/4; 25%) toxins. Two isolates from the kashar cheese samples (2/4; 50%) were found to be enterotoxigenic; one produced SEA (1/2; 50%) and the other produced SED (1/2; 50%). One isolate from the butter samples (1/4; 25%) showed enterotoxigenic character (SEB, 1/1; 100%). The products were found to be potentially hazardous to public health because of the fact that levels of contamination were higher than 105-106 cfu/g ml in 39% (25/64, 17 raw milk, 7 white cheese, and 1 butter) of the analyzed samples.
    Mesh-Begriff(e) Animals ; Colony Count, Microbial ; DNA, Bacterial/genetics ; DNA, Bacterial/isolation & purification ; Dairy Products/analysis ; Dairy Products/microbiology ; Enterotoxins/genetics ; Enterotoxins/isolation & purification ; Food Contamination/analysis ; Food Microbiology ; Milk/chemistry ; Milk/microbiology ; Multiplex Polymerase Chain Reaction/methods ; RNA, Ribosomal, 16S/genetics ; RNA, Ribosomal, 16S/isolation & purification ; Staphylococcus aureus/genetics ; Staphylococcus aureus/isolation & purification ; Turkey
    Chemische Substanzen DNA, Bacterial ; Enterotoxins ; RNA, Ribosomal, 16S ; enterotoxin D, Staphylococcal (12788-99-7) ; enterotoxin A, Staphylococcal (37337-57-8) ; enterotoxin B, staphylococcal (39424-53-8) ; enterotoxin C, staphylococcal (39424-54-9)
    Sprache Englisch
    Erscheinungsdatum 2012-11
    Erscheinungsland United States
    Dokumenttyp Journal Article
    ZDB-ID 241615-3
    ISSN 1750-3841 ; 0022-1147
    ISSN (online) 1750-3841
    ISSN 0022-1147
    DOI 10.1111/j.1750-3841.2012.02954.x
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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  10. Artikel: Classical enterotoxigenic characteristics of Staphylococcus aureus strains isolated from bovine subclinical mastitis in Van, Turkey.

    Boynukara, Banur / Gulhan, Timur / Alisarli, Mustafa / Gurturk, Kemal / Solmaz, Hasan

    International journal of food microbiology

    2008  Band 125, Heft 2, Seite(n) 209–211

    Abstract: The aim of the present study was to investigate classical enterotoxigenic properties of Staphylococcus aureus strains isolated from cows with subclinical mastitis. For this purpose, 480 milk samples from cows with subclinical mastitis raised in different ...

    Abstract The aim of the present study was to investigate classical enterotoxigenic properties of Staphylococcus aureus strains isolated from cows with subclinical mastitis. For this purpose, 480 milk samples from cows with subclinical mastitis raised in different villages neighbouring Van city Center were collected. A total of 106 S. aureus strains were isolated. Twenty seven isolates (25.5%) were found to be enterotoxigenic by reverse passive latex agglutination (RPLA). Of these, 25 (23.6%) were positive for staphylococcal enterotoxin A (SEA), 2 (1.9%) for SEB. None of the isolates was positive for SEC or SED. This study showed that most S. aureus strains isolated from bovine subclinical mastitis produced SEA compared to other SEs.
    Mesh-Begriff(e) Animals ; Cattle ; Consumer Product Safety ; Enterotoxins/biosynthesis ; Female ; Food Contamination/analysis ; Food Contamination/prevention & control ; Food Microbiology ; Humans ; Mastitis, Bovine/microbiology ; Milk/microbiology ; Staphylococcal Food Poisoning/microbiology ; Staphylococcal Food Poisoning/prevention & control ; Staphylococcal Infections/microbiology ; Staphylococcal Infections/veterinary ; Staphylococcus aureus/metabolism ; Turkey
    Chemische Substanzen Enterotoxins ; enterotoxin A, Staphylococcal (37337-57-8) ; enterotoxin B, staphylococcal (39424-53-8)
    Sprache Englisch
    Erscheinungsdatum 2008-07-15
    Erscheinungsland Netherlands
    Dokumenttyp Journal Article
    ZDB-ID 87122-9
    ISSN 1879-3460 ; 0168-1605
    ISSN (online) 1879-3460
    ISSN 0168-1605
    DOI 10.1016/j.ijfoodmicro.2008.03.024
    Datenquelle MEDical Literature Analysis and Retrieval System OnLINE

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