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  1. Article ; Online: Saliva sampling method influences oral microbiome composition and taxa distribution associated with oral diseases.

    Roca, Cristian / Alkhateeb, Alaa A / Deanhardt, Bryson K / Macdonald, Jade K / Chi, Donald L / Wang, Jeremy R / Wolfgang, Matthew C

    PloS one

    2024  Volume 19, Issue 3, Page(s) e0301016

    Abstract: Saliva is a readily accessible and inexpensive biological specimen that enables investigation of the oral microbiome, which can serve as a biomarker of oral and systemic health. There are two routine approaches to collect saliva, stimulated and ... ...

    Abstract Saliva is a readily accessible and inexpensive biological specimen that enables investigation of the oral microbiome, which can serve as a biomarker of oral and systemic health. There are two routine approaches to collect saliva, stimulated and unstimulated; however, there is no consensus on how sampling method influences oral microbiome metrics. In this study, we analyzed paired saliva samples (unstimulated and stimulated) from 88 individuals, aged 7-18 years. Using 16S rRNA gene sequencing, we investigated the differences in bacterial microbiome composition between sample types and determined how sampling method affects the distribution of taxa associated with untreated dental caries and gingivitis. Our analyses indicated significant differences in microbiome composition between the sample types. Both sampling methods were able to detect significant differences in microbiome composition between healthy subjects and subjects with untreated caries. However, only stimulated saliva revealed a significant association between microbiome diversity and composition in individuals with diagnosed gingivitis. Furthermore, taxa previously associated with dental caries and gingivitis were preferentially enriched in individuals with each respective disease only in stimulated saliva. Our study suggests that stimulated saliva provides a more nuanced readout of microbiome composition and taxa distribution associated with untreated dental caries and gingivitis compared to unstimulated saliva.
    MeSH term(s) Humans ; Saliva/microbiology ; RNA, Ribosomal, 16S/genetics ; Dental Caries ; Microbiota/genetics ; Gingivitis
    Chemical Substances RNA, Ribosomal, 16S
    Language English
    Publishing date 2024-03-28
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2267670-3
    ISSN 1932-6203 ; 1932-6203
    ISSN (online) 1932-6203
    ISSN 1932-6203
    DOI 10.1371/journal.pone.0301016
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Untreated dental disease and lung transplant waitlist evaluation time for individuals with cystic fibrosis.

    Alkhateeb, Alaa A / Lease, Erika D / Mancl, Lloyd A / Chi, Donald L

    Special care in dentistry : official publication of the American Association of Hospital Dentists, the Academy of Dentistry for the Handicapped, and the American Society for Geriatric Dentistry

    2021  Volume 41, Issue 4, Page(s) 489–497

    Abstract: Aims: Dental clearance is typically part of the evaluation process prior to placement on the lung transplant waiting list. Individuals with cystic fibrosis (CF) are thought to be at low risk for dental disease. We hypothesized that individuals with CF ... ...

    Abstract Aims: Dental clearance is typically part of the evaluation process prior to placement on the lung transplant waiting list. Individuals with cystic fibrosis (CF) are thought to be at low risk for dental disease. We hypothesized that individuals with CF in need of lung transplantation would have lower dental disease prevalence and shorter waitlist evaluation time than individuals with non-CF lung diseases.
    Methods and results: We conducted a retrospective study of individuals who received a lung transplant between 2011 and 2017 at the University of Washington (Seattle, WA, USA) (N = 280). Untreated dental disease was assessed by the individual's dentist. Waitlist evaluation time was defined as the time, in days, from the initial evaluation by a transplant pulmonologist to placement on the lung transplant waiting list. We used logistic and linear regression models for hypothesis testing. The prevalence of untreated dental disease did not differ by CF status (p = 0.99). There was no difference in waitlist evaluation time for transplant recipients by CF status (p = 0.78) or by dental disease status (p = 0.93).
    Conclusions: Our findings provide further evidence that individuals with CF are not at low risk for dental disease. Ensuring optimal oral health is important for all individuals with lung diseases.
    MeSH term(s) Cystic Fibrosis ; Humans ; Lung Transplantation ; Retrospective Studies ; Stomatognathic Diseases ; Waiting Lists
    Language English
    Publishing date 2021-03-22
    Publishing country United States
    Document type Journal Article
    ZDB-ID 604118-8
    ISSN 1754-4505 ; 0275-1879
    ISSN (online) 1754-4505
    ISSN 0275-1879
    DOI 10.1111/scd.12586
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Periodontitis Risk Factors in Adults with Cystic Fibrosis: A pilot study.

    Alkhateeb, Alaa A / Mancl, Lloyd A / Ramos, Kathleen J / Rothen, Marilynn L / Kotsakis, Georgios A / Trence, Dace L / Chi, Donald L

    Journal of dental hygiene : JDH

    2023  Volume 97, Issue 2, Page(s) 7–21

    Abstract: ... ...

    Abstract Purpose
    MeSH term(s) Adult ; Humans ; Cystic Fibrosis/complications ; Cystic Fibrosis/epidemiology ; Pilot Projects ; Cross-Sectional Studies ; Risk Factors ; Periodontitis/complications ; Periodontitis/epidemiology
    Language English
    Publishing date 2023-04-18
    Publishing country United States
    Document type Journal Article
    ZDB-ID 1035719-1
    ISSN 1553-0205 ; 1043-254X
    ISSN (online) 1553-0205
    ISSN 1043-254X
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.B 1831: Show issues Location:
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  4. Article ; Online: Unstimulated Saliva-Related Caries Risk Factors in Individuals with Cystic Fibrosis: A Cross-Sectional Analysis of Unstimulated Salivary Flow, pH, and Buffering Capacity.

    Alkhateeb, Alaa A / Mancl, Lloyd A / Presland, Richard B / Rothen, Marilynn L / Chi, Donald L

    Caries research

    2016  Volume 51, Issue 1, Page(s) 1–6

    Abstract: Salivary flow rate, pH, and buffering capacity are associated with dental caries, but studies from the cystic fibrosis (CF) literature are inconclusive regarding these salivary factors and caries. The aim of this study was to evaluate these factors and ... ...

    Abstract Salivary flow rate, pH, and buffering capacity are associated with dental caries, but studies from the cystic fibrosis (CF) literature are inconclusive regarding these salivary factors and caries. The aim of this study was to evaluate these factors and their associations with dental caries in individuals with CF. Unstimulated whole saliva was collected from individuals aged 6-20 years at Seattle Children's Hospital CF Clinic, USA (n = 83). Salivary flow rate was measured in milliliters per minute. Salivary pH was assessed using a laboratory pH meter. Buffering capacity was assessed by titration with HCl. The outcome measure was caries prevalence, defined as the number of decayed, missing, or filled primary and permanent tooth surfaces. Spearman's rank correlation coefficient and the t test were used to test for bivariate associations. Multiple variable linear regression models were used to (1) run confounder-adjusted analyses and (2) assess for potential interactions. There was no significant association between salivary flow rate or buffering capacity and caries prevalence. There was a significant negative association between salivary pH and caries prevalence, but this association was no longer significant after adjusting for age. There was no significant interaction between salivary flow rate and buffering capacity or between antibiotic use and the 3 salivary factors. Our results indicate that unstimulated salivary factors are not associated with dental caries prevalence in individuals with CF. Future studies should investigate other potential saliva-related caries risk factors in individuals with CF such as cariogenic bacteria levels, salivary host defense peptide levels, and medication use.
    MeSH term(s) Adolescent ; Child ; Cross-Sectional Studies ; Cystic Fibrosis/epidemiology ; DMF Index ; Dental Caries/epidemiology ; Female ; Humans ; Hydrogen-Ion Concentration ; Linear Models ; Male ; Prevalence ; Risk Factors ; Saliva/chemistry ; Saliva/metabolism ; Secretory Rate/physiology ; United States/epidemiology ; Young Adult
    Language English
    Publishing date 2016-11-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 390575-5
    ISSN 1421-976X ; 0008-6568
    ISSN (online) 1421-976X
    ISSN 0008-6568
    DOI 10.1159/000450658
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 556: Show issues Location:
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article: Unstimulated Saliva-Related Caries Risk Factors in Individuals with Cystic Fibrosis: A Cross-Sectional Analysis of Unstimulated Salivary Flow, pH, and Buffering Capacity

    Alkhateeb, Alaa A. / Mancl, Lloyd A. / Presland, Richard B. / Rothen, Marilynn L. / Chi, Donald L.

    Caries Research

    2016  Volume 51, Issue 1, Page(s) 1–6

    Abstract: Salivary flow rate, pH, and buffering capacity are associated with dental caries, but studies from the cystic fibrosis (CF) literature are inconclusive regarding these salivary factors and caries. The aim of this study was to evaluate these factors and ... ...

    Institution Department of Oral Health Sciences, School of Dentistry, and Division of Dermatology, School of Medicine, University of Washington, Seattle, Wash., USA
    Abstract Salivary flow rate, pH, and buffering capacity are associated with dental caries, but studies from the cystic fibrosis (CF) literature are inconclusive regarding these salivary factors and caries. The aim of this study was to evaluate these factors and their associations with dental caries in individuals with CF. Unstimulated whole saliva was collected from individuals aged 6-20 years at Seattle Children's Hospital CF Clinic, USA (n = 83). Salivary flow rate was measured in milliliters per minute. Salivary pH was assessed using a laboratory pH meter. Buffering capacity was assessed by titration with HCl. The outcome measure was caries prevalence, defined as the number of decayed, missing, or filled primary and permanent tooth surfaces. Spearman's rank correlation coefficient and the t test were used to test for bivariate associations. Multiple variable linear regression models were used to (1) run confounder-adjusted analyses and (2) assess for potential interactions. There was no significant association between salivary flow rate or buffering capacity and caries prevalence. There was a significant negative association between salivary pH and caries prevalence, but this association was no longer significant after adjusting for age. There was no significant interaction between salivary flow rate and buffering capacity or between antibiotic use and the 3 salivary factors. Our results indicate that unstimulated salivary factors are not associated with dental caries prevalence in individuals with CF. Future studies should investigate other potential saliva-related caries risk factors in individuals with CF such as cariogenic bacteria levels, salivary host defense peptide levels, and medication use.
    Keywords Buffering capacity ; Cystic fibrosis ; Dental caries ; pH ; Salivary flow ; Unstimulated saliva
    Language English
    Publishing date 2016-11-16
    Publisher S. Karger AG
    Publishing place Basel, Switzerland
    Document type Article
    Note Original Paper
    ZDB-ID 390575-5
    ISSN 1421-976X ; 0008-6568
    ISSN (online) 1421-976X
    ISSN 0008-6568
    DOI 10.1159/000450658
    Database Karger publisher's database

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 556: Show issues Location:
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