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  1. Article: Differential Colonization of the Plant Vasculature Between Endophytic Versus Pathogenic

    Martínez-Soto, Domingo / Yu, Houlin / Allen, Kelly S / Ma, Li-Jun

    Molecular plant-microbe interactions : MPMI

    2023  Volume 36, Issue 1, Page(s) 4–13

    Abstract: Plant xylem colonization is the hallmark of vascular wilt diseases caused by phytopathogens within ... ...

    Abstract Plant xylem colonization is the hallmark of vascular wilt diseases caused by phytopathogens within the
    MeSH term(s) Arabidopsis ; Lignin ; Plant Diseases/microbiology ; Fusarium/genetics ; Solanum lycopersicum ; Plant Roots/microbiology
    Chemical Substances Lignin (9005-53-2)
    Language English
    Publishing date 2023-01-09
    Publishing country United States
    Document type Journal Article
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-08-22-0166-SC
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Differential Colonization of the Plant Vasculature Between Endophytic Versus Pathogenic Fusarium oxysporum Strains

    Martínez Soto, Domingo / Yu, Houlin / Allen, Kelly S. / Ma, Li-Jun

    Molecular Plant-Microbe Interactions. 2023 Jan., v. 36, no. 1 p.4-13

    2023  

    Abstract: Plant xylem colonization is the hallmark of vascular wilt diseases caused by phytopathogens within the Fusarium oxysporum species complex. Recently, xylem colonization has also been reported among endophytic F. oxysporum strains, resulting in some ... ...

    Abstract Plant xylem colonization is the hallmark of vascular wilt diseases caused by phytopathogens within the Fusarium oxysporum species complex. Recently, xylem colonization has also been reported among endophytic F. oxysporum strains, resulting in some uncertainty. This study compares xylem colonization processes by pathogenic versus endophytic strains in Arabidopsis thaliana and Solanum lycopersicum, using Arabidopsis pathogen Fo5176, tomato pathogen Fol4287, and the endophyte Fo47, which can colonize both plant hosts. We observed that all strains were able to advance from epidermis to endodermis within 3 days postinoculation (dpi) and reached the root xylem at 4 dpi. However, this shared progression was restricted to lateral roots and the elongation zone of the primary root. Only pathogens reached the xylem above the primary-root maturation zone (PMZ). Related to the distinct colonization patterns, we also observed stronger induction of callose at the PMZ and lignin deposition at primary-lateral root junctions by the endophyte in both plants. This observation was further supported by stronger induction of Arabidopsis genes involved in callose and lignin biosynthesis during the endophytic colonization (Fo47) compared with the pathogenic interaction (Fo5176). Moreover, both pathogens encode more plant cell wall–degrading enzymes than the endophyte Fo47. Therefore, observed differences in callose and lignin deposition could be the combination of host production and the subsequent fungal degradation. In summary, this study demonstrates spatial differences between endophytic and pathogenic colonization, strongly suggesting that further investigations of molecular arm-races are needed to understand how plants differentiate friend from foe. Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
    Keywords Arabidopsis thaliana ; Fusarium oxysporum ; Solanum lycopersicum ; biosynthesis ; callose ; endodermis ; endophytes ; fungi ; lignin ; plant pathogens ; tomatoes ; uncertainty ; vascular wilt ; xylem ; differential plant colonization ; endophytic fungus ; Fusarium oxysporum Fo47 ; pathogenic fungus ; plant-fungus interaction
    Language English
    Dates of publication 2023-01
    Size p. 4-13.
    Publishing place The American Phytopathological Society
    Document type Article ; Online
    ZDB-ID 743331-1
    ISSN 1943-7706 ; 0894-0282
    ISSN (online) 1943-7706
    ISSN 0894-0282
    DOI 10.1094/MPMI-08-22-0166-SC
    Database NAL-Catalogue (AGRICOLA)

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  3. Article: A review of nursery production systems and their influence on urban tree survival

    Allen, Kelly S / Bayer, Amanda / Brazee, Nicholas J / Harper, Richard W

    Urban forestry & urban greening. 2017 Jan., v. 21

    2017  

    Abstract: Urban trees face a myriad of complex challenges growing in the built environment. The most common environmental conditions influencing urban tree mortality are water availability, nutrient deficiency and soil compaction. Long-term survival of recently ... ...

    Abstract Urban trees face a myriad of complex challenges growing in the built environment. The most common environmental conditions influencing urban tree mortality are water availability, nutrient deficiency and soil compaction. Long-term survival of recently installed trees has been directly attributed to site conditions, planting technique, and post-transplant maintenance. Tree survival is also dependent on selection of healthy, suitable plant material. Production methods for woody plants include traditional plastic containers (CG), pot-in-pot containers (PIP), and in-ground fabric containers (IGF). Field grown trees may be produced as bare-root (BR) or root ball-excavated and burlap-wrapped (B&B) trees. Each of these methods offers unique advantages in relation to production and installation. Many of the studies reviewed reveal varying post-transplant establishment and survival responses to production methods at a species-specific level.
    Keywords containers ; environmental factors ; nutrient deficiencies ; planting ; production technology ; soil compaction ; somatomedins ; tree mortality ; trees ; woody plants
    Language English
    Dates of publication 2017-01
    Size p. 183-191.
    Publishing place Elsevier GmbH
    Document type Article
    ISSN 1618-8667
    DOI 10.1016/j.ufug.2016.12.002
    Database NAL-Catalogue (AGRICOLA)

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  4. Article: A De Novo-Assembly Based Data Analysis Pipeline for Plant Obligate Parasite Metatranscriptomic Studies.

    Guo, Li / Allen, Kelly S / Deiulio, Greg / Zhang, Yong / Madeiras, Angela M / Wick, Robert L / Ma, Li-Jun

    Frontiers in plant science

    2016  Volume 7, Page(s) 925

    Abstract: Current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety. These obligate parasites are typically unculturable in the laboratory, posing ... ...

    Abstract Current and emerging plant diseases caused by obligate parasitic microbes such as rusts, downy mildews, and powdery mildews threaten worldwide crop production and food safety. These obligate parasites are typically unculturable in the laboratory, posing technical challenges to characterize them at the genetic and genomic level. Here we have developed a data analysis pipeline integrating several bioinformatic software programs. This pipeline facilitates rapid gene discovery and expression analysis of a plant host and its obligate parasite simultaneously by next generation sequencing of mixed host and pathogen RNA (i.e., metatranscriptomics). We applied this pipeline to metatranscriptomic sequencing data of sweet basil (Ocimum basilicum) and its obligate downy mildew parasite Peronospora belbahrii, both lacking a sequenced genome. Even with a single data point, we were able to identify both candidate host defense genes and pathogen virulence genes that are highly expressed during infection. This demonstrates the power of this pipeline for identifying genes important in host-pathogen interactions without prior genomic information for either the plant host or the obligate biotrophic pathogen. The simplicity of this pipeline makes it accessible to researchers with limited computational skills and applicable to metatranscriptomic data analysis in a wide range of plant-obligate-parasite systems.
    Language English
    Publishing date 2016-07-11
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2613694-6
    ISSN 1664-462X
    ISSN 1664-462X
    DOI 10.3389/fpls.2016.00925
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  5. Article: Localized attentional interference reflects competition for reentrant processing.

    Steelman-Allen, Kelly S / McCarley, Jason S / Mounts, Jeffrey R W

    Psychonomic bulletin & review

    2009  Volume 16, Issue 1, Page(s) 110–115

    Abstract: Visual performance is compromised when attention is divided between objects that are near one another in the visual field. It has been postulated that this effect, termed localized attentional interference (LAI), reflects competition between visual- ... ...

    Abstract Visual performance is compromised when attention is divided between objects that are near one another in the visual field. It has been postulated that this effect, termed localized attentional interference (LAI), reflects competition between visual-object representations for the control of cortical neural responses. To determine whether LAI arises during feedforward processing or during reentrant processing, the present study examined the influence of poststimulus pattern and four-dot masks on the strength of the effect. Experiment 1 found that pattern masks, which are believed to compromise feedforward processing, do not produce stronger LAI than do four-dot masks, which are believed to leave feedforward processing undisrupted. Experiment 2 found that LAI is weaker when reentrant processing is interrupted shortly after initiation than it is when reentrant processing is allowed to run to completion. The results suggest that LAI emerges from competition between objects during reentrant processing.
    MeSH term(s) Attention ; Color Perception ; Cues ; Discrimination Learning ; Female ; Humans ; Male ; Orientation ; Pattern Recognition, Visual ; Perceptual Masking ; Visual Fields ; Young Adult
    Language English
    Publishing date 2009-01-14
    Publishing country United States
    Document type Journal Article
    ZDB-ID 2031311-1
    ISSN 1531-5320 ; 1069-9384
    ISSN (online) 1531-5320
    ISSN 1069-9384
    DOI 10.3758/PBR.16.1.110
    Database MEDical Literature Analysis and Retrieval System OnLINE

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