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  1. Article ; Online: Pretherapeutic Comparative Dosimetry of

    Feuerecker, Benedikt / Chantadisai, Maythinee / Allmann, Anne / Tauber, Robert / Allmann, Jakob / Steinhelfer, Lisa / Rauscher, Isabel / Wurzer, Alexander / Wester, Hans-Jürgen / Weber, Wolfgang A / d'Alessandria, Calogero / Eiber, Matthias

    Journal of nuclear medicine : official publication, Society of Nuclear Medicine

    2021  Volume 63, Issue 6, Page(s) 833–839

    Abstract: Radiohybrid prostate-specific membrane antigen (rhPSMA) ligands allow for labeling ... ...

    Abstract Radiohybrid prostate-specific membrane antigen (rhPSMA) ligands allow for labeling with
    MeSH term(s) Dipeptides/therapeutic use ; Heterocyclic Compounds, 1-Ring/therapeutic use ; Humans ; Ligands ; Lutetium/therapeutic use ; Male ; Prostate-Specific Antigen ; Prostatic Neoplasms, Castration-Resistant/pathology ; Radiopharmaceuticals/adverse effects ; Retrospective Studies ; Tomography, X-Ray Computed ; Urea/analogs & derivatives
    Chemical Substances Dipeptides ; Heterocyclic Compounds, 1-Ring ; Ligands ; PSMA I&T ; Radiopharmaceuticals ; Lutetium (5H0DOZ21UJ) ; Urea (8W8T17847W) ; Prostate-Specific Antigen (EC 3.4.21.77)
    Language English
    Publishing date 2021-09-16
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80272-4
    ISSN 1535-5667 ; 0097-9058 ; 0161-5505 ; 0022-3123
    ISSN (online) 1535-5667
    ISSN 0097-9058 ; 0161-5505 ; 0022-3123
    DOI 10.2967/jnumed.121.262671
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 114: Show issues Location:
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    Zs.MO 328: Show issues

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  2. Article: Structural basis for a lethal mutation in U6 RNA.

    Sashital, Dipali G / Allmann, Anne M / Van Doren, Steven R / Butcher, Samuel E

    Biochemistry

    2003  Volume 42, Issue 6, Page(s) 1470–1477

    Abstract: U6 RNA is essential for nuclear pre-mRNA splicing and has been implicated directly in catalysis of intron removal. The U80G mutation at the essential magnesium binding site of the U6 3' intramolecular stem-loop region (ISL) is lethal in yeast. To further ...

    Abstract U6 RNA is essential for nuclear pre-mRNA splicing and has been implicated directly in catalysis of intron removal. The U80G mutation at the essential magnesium binding site of the U6 3' intramolecular stem-loop region (ISL) is lethal in yeast. To further understand the structure and function of the U6 ISL, we have investigated the structural basis for the lethal U80G mutation by NMR and optical spectroscopy. The NMR structure reveals that the U80G mutation causes a structural rearrangement within the ISL resulting in the formation of a new Watson-Crick base pair (C67 x G80), and disrupts a protonated C67 x A79 wobble pair that forms in the wild-type structure. Despite the structural change, the accessibility of the metal binding site is unperturbed, and cadmium titration produces similar phosphorus chemical shift changes for both the U80G mutant and wild-type RNAs. The thermodynamic stability of the U80G mutant is significantly increased (Delta Delta G(fold) = -3.6 +/- 1.9 kcal/mol), consistent with formation of the Watson-Crick pair. Our structural and thermodynamic data, in combination with previous genetic data, suggest that the lethal basis for the U80G mutation is stem-loop hyperstabilization. This hyperstabilization may prevent the U6 ISL melting and rearrangement necessary for association with U4.
    MeSH term(s) Base Pairing/genetics ; Binding Sites/genetics ; Cadmium/chemistry ; Crystallography, X-Ray ; Genes, Lethal/genetics ; Guanine/chemistry ; Guanine/metabolism ; Hydrogen Bonding ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; Point Mutation ; Protons ; RNA Stability/genetics ; RNA, Small Nuclear/chemistry ; RNA, Small Nuclear/genetics ; RNA, Small Nuclear/metabolism ; Spectrophotometry, Ultraviolet ; Thermodynamics ; Uracil/chemistry ; Uracil/metabolism
    Chemical Substances Protons ; RNA, Small Nuclear ; U6 small nuclear RNA ; Cadmium (00BH33GNGH) ; Uracil (56HH86ZVCT) ; Guanine (5Z93L87A1R)
    Language English
    Publishing date 2003-02-18
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1108-3
    ISSN 1520-4995 ; 0006-2960
    ISSN (online) 1520-4995
    ISSN 0006-2960
    DOI 10.1021/bi027137h
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 217: Show issues Location:
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  3. Article: Structure of the U6 RNA intramolecular stem-loop harboring an S(P)-phosphorothioate modification.

    Reiter, Nicholas J / Nikstad, Laura J / Allmann, Anne M / Johnson, Randal J / Butcher, Samuel E

    RNA (New York, N.Y.)

    2003  Volume 9, Issue 5, Page(s) 533–542

    Abstract: Phosphorothioate-substitution experiments are often used to elucidate functionally important metal ion-binding sites on RNA. All previous experiments with S(P)-phosphorothioate-substituted RNAs have been done in the absence of structural information for ... ...

    Abstract Phosphorothioate-substitution experiments are often used to elucidate functionally important metal ion-binding sites on RNA. All previous experiments with S(P)-phosphorothioate-substituted RNAs have been done in the absence of structural information for this particular diastereomer. Yeast U6 RNA contains a metal ion-binding site that is essential for spliceosome function and includes the pro-S(P) oxygen 5' of U(80). S(P)-phosphorothioate substitution at this location creates spliceosomes dependent on thiophilic ions for the first step of splicing. We have determined the solution structure of the U(80) S(P)-phosphorothioate-substituted U6 intramolecular stem-loop (ISL), and also report the refined NMR structure of the unmodified U6 ISL. Both structures were determined with inclusion of (1)H-(13)C residual dipolar couplings. The precision of the structures with and without phosphorothioate (RMSD = 1.05 and 0.79 A, respectively) allows comparison of the local and long-range structural effect of the modification. We find that the U6-ISL structure is unperturbed by the phosphorothioate. Additionally, the thermodynamic stability of the U6 ISL is dependent on the protonation state of the A(79)-C(67) wobble pair and is not affected by the adjacent phosphorothioate. These results indicate that a single S(P)-phosphorothioate substitution can be structurally benign, and further validate the metal ion rescue experiments used to identify the essential metal-binding site(s) in the spliceosome.
    MeSH term(s) Base Sequence ; Binding Sites ; Metals/metabolism ; Models, Molecular ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; RNA, Fungal/chemistry ; RNA, Fungal/genetics ; RNA, Small Nuclear/chemistry ; RNA, Small Nuclear/genetics ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; Spliceosomes/metabolism ; Thermodynamics ; Thionucleotides/chemistry
    Chemical Substances Metals ; RNA, Fungal ; RNA, Small Nuclear ; Thionucleotides ; U6 small nuclear RNA
    Language English
    Publishing date 2003-01-04
    Publishing country United States
    Document type Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1241540-6
    ISSN 1355-8382
    ISSN 1355-8382
    DOI 10.1261/rna.2199103
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Metal binding and base ionization in the U6 RNA intramolecular stem-loop structure.

    Huppler, Anna / Nikstad, Laura J / Allmann, Anne M / Brow, David A / Butcher, Samuel E

    Nature structural biology

    2002  Volume 9, Issue 6, Page(s) 431–435

    Abstract: U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first ...

    Abstract U6 RNA is a key component of the catalytic core of the spliceosome. A metal ion essential for the first catalytic step of pre-mRNA splicing binds to the U80 Sp phosphate oxygen within the yeast U6 intramolecular stem-loop (ISL). Here we present the first structural data for U6 RNA, revealing the three-dimensional structure of the highly conserved U6 ISL. The ISL binds metal ion at the U80 site with the same stereo specificity as the intact spliceosome. The metal-binding site is adjacent to a readily protonated C.A wobble pair. Protonation of the C.A pair and metal binding are mutually antagonistic. These results support a ribozyme model for U6 RNA function and suggest a possible mechanism for the regulation of RNA splicing.
    MeSH term(s) Base Sequence ; Binding Sites ; Evolution, Molecular ; Hydrogen-Ion Concentration ; Ions ; Metals/metabolism ; Molecular Sequence Data ; Nuclear Magnetic Resonance, Biomolecular ; Nucleic Acid Conformation ; Protons ; RNA Splicing ; RNA, Fungal/chemistry ; RNA, Fungal/genetics ; RNA, Fungal/metabolism ; RNA, Small Nuclear/chemistry ; RNA, Small Nuclear/genetics ; RNA, Small Nuclear/metabolism ; Saccharomyces cerevisiae/genetics ; Static Electricity ; Structure-Activity Relationship ; Substrate Specificity
    Chemical Substances Ions ; Metals ; Protons ; RNA, Fungal ; RNA, Small Nuclear ; U6 small nuclear RNA
    Language English
    Publishing date 2002-06
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 1192623-5
    ISSN 1072-8368
    ISSN 1072-8368
    DOI 10.1038/nsb800
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    Zs.A 4101: Show issues Location:
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