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  1. Article: Specificity of Ca

    Mokelke, Eric A / Alloosh, Mouhamad / Sturek, Michael

    Current topics in membranes

    2022  Volume 90, Page(s) 123–139

    Abstract: Vascular smooth muscle cells express several isoforms of a number of classes of ... ...

    Abstract Vascular smooth muscle cells express several isoforms of a number of classes of K
    MeSH term(s) Swine ; Animals ; Muscle, Smooth, Vascular/physiology ; Potassium Channels/metabolism ; Membrane Potentials ; Muscle Contraction ; Atherosclerosis/metabolism
    Chemical Substances Potassium Channels
    Language English
    Publishing date 2022-10-22
    Publishing country United States
    Document type Review ; Journal Article ; Research Support, N.I.H., Extramural
    ISSN 1063-5823
    ISSN 1063-5823
    DOI 10.1016/bs.ctm.2022.09.005
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Swine Disease Models for Optimal Vascular Engineering.

    Sturek, Michael / Alloosh, Mouhamad / Sellke, Frank W

    Annual review of biomedical engineering

    2020  Volume 22, Page(s) 25–49

    Abstract: Swine disease models are essential for mimicry of human metabolic and vascular pathophysiology, thereby enabling high-fidelity translation to human medicine. The worldwide epidemic of obesity, metabolic disease, and diabetes has prompted the focus on ... ...

    Abstract Swine disease models are essential for mimicry of human metabolic and vascular pathophysiology, thereby enabling high-fidelity translation to human medicine. The worldwide epidemic of obesity, metabolic disease, and diabetes has prompted the focus on these diseases in this review. We highlight the remarkable similarity between Ossabaw miniature swine and humans with metabolic syndrome and atherosclerosis. Although the evidence is strongest for swine models of coronary artery disease, findings are generally applicable to any vascular bed. We discuss the major strengths and weaknesses of swine models. The development of vascular imaging is an example of optimal vascular engineering in swine. Although challenges regarding infrastructure and training of engineers in the use of swine models exist, opportunities are ripe for gene editing, studies of molecular mechanisms, and use of swine in coronary artery imaging and testing of devices that can move quickly to human clinical studies.
    MeSH term(s) Animals ; Atherosclerosis/physiopathology ; Blood Vessel Prosthesis ; Coronary Artery Disease/metabolism ; Disease Models, Animal ; Dyslipidemias/metabolism ; Female ; Genome ; Humans ; Insulin Resistance ; Kidney/pathology ; Male ; Metabolic Syndrome/physiopathology ; Obesity/metabolism ; Sequence Analysis, DNA ; Tissue Engineering/methods
    Language English
    Publishing date 2020-03-02
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 1448425-0
    ISSN 1545-4274 ; 1523-9829
    ISSN (online) 1545-4274
    ISSN 1523-9829
    DOI 10.1146/annurev-bioeng-082919-053009
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  3. Article: Animal Models for COVID-19: More to the Picture Than ACE2, Rodents, Ferrets, and Non-human Primates. A Case for Porcine Respiratory Coronavirus and the Obese Ossabaw Pig

    Heegaard, P. M. H. / Sturek, M. / Alloosh, M. / Belsham, G. J.

    Frontiers in Microbiology

    Abstract: The ongoing COVID-19 pandemic caused by infection with SARS-CoV-2 has created an urgent need for animal models to enable study of basic infection and disease mechanisms and for development of vaccines, therapeutics, and diagnostics Most research on ... ...

    Abstract The ongoing COVID-19 pandemic caused by infection with SARS-CoV-2 has created an urgent need for animal models to enable study of basic infection and disease mechanisms and for development of vaccines, therapeutics, and diagnostics Most research on animal models for COVID-19 has been directed toward rodents, transgenic rodents, and non-human primates The primary focus has been on the angiotensin-converting enzyme 2 (ACE2), which is a host cell receptor for SARS-CoV-2 Among investigated species, irrespective of ACE2 spike protein binding, only mild (or no) disease has occurred following infection with SARS-CoV-2, suggesting that ACE2 may be necessary for infection but is not sufficient to determine the outcome of infection The common trait of all species investigated as COVID models is their healthy status prior to virus challenge In contrast, the vast majority of severe COVID-19 cases occur in people with chronic comorbidities such as diabetes, obesity, and/or cardiovascular disease Healthy pigs express ACE2 protein that binds the viral spike protein but they are not susceptible to infection with SARS-CoV-2 However, certain pig breeds, such as the Ossabaw pig, can reproducibly be made obese and show most aspects of the metabolic syndrome, thus resembling the more than 80% of the critically ill COVID-19 patients admitted to hospitals We urge considering infection with porcine respiratory coronavirus of metabolic syndrome pigs, such as the obese Ossabaw pig, as a highly relevant animal model of severe COVID-19
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #874505
    Database COVID19

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  4. Article: Genetic diversity of Fusarium oxysporum f. sp. ciceris isolates affecting chickpea in Syria

    Alloosh, Mysaa / Hamwieh, Aladdin / Ahmed, Seid / Alkai, Bassel

    Crop protection. 2019 June 24,

    2019  

    Abstract: Fusarium wilt (Fusarium oxysporum f.sp. ciceris) is the most important soil-borne disease of chickpea in Syria. Seventy isolates of the wilt pathogen were isolated from diseased plant samples collected from farmers’ fields and research centers in Syria, ... ...

    Abstract Fusarium wilt (Fusarium oxysporum f.sp. ciceris) is the most important soil-borne disease of chickpea in Syria. Seventy isolates of the wilt pathogen were isolated from diseased plant samples collected from farmers’ fields and research centers in Syria, and a research station in Lebanon, were studied for their genetic diversity using random amplification of polymorphic DNA (RAPD), simple sequence repeat (SSR) markers and sequence characterized amplified region (SCAR) molecular markers. High genetic diversity within populations and low among populations was observed. The cluster analyses grouped the isolates into seven clusters and the STRUCTURE analyses showed three populations. Using race-specific markers, four races (0, 1B/C, 5 and 6) were identified and 12 isolates were not designated to any of the known races. The dominant races were 0 and 1B/C in the pathogen population where the former was dominant in both spring- and winter-planted chickpea crops. This study showed that ICARDA breeding lines are being evaluated against mixed races and populations. Hence elite chickpea lines distributed to national partners carry resistance to many races and populations prevalent in the Mediterranean regions.
    Keywords DNA ; Fusarium oxysporum ; Fusarium wilt ; breeding lines ; chickpeas ; cluster analysis ; crops ; farmers ; genetic markers ; genetic variation ; microsatellite repeats ; races ; random amplified polymorphic DNA technique ; soil-borne diseases ; spring ; Lebanon ; Syria
    Language English
    Dates of publication 2019-0624
    Publishing place Elsevier Ltd
    Document type Article
    Note Pre-press version
    ZDB-ID 786839-x
    ISSN 1873-6904 ; 0261-2194
    ISSN (online) 1873-6904
    ISSN 0261-2194
    DOI 10.1016/j.cropro.2019.104863
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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  5. Article: Animal Models for COVID-19: More to the Picture Than ACE2, Rodents, Ferrets, and Non-human Primates. A Case for Porcine Respiratory Coronavirus and the Obese Ossabaw Pig.

    Heegaard, Peter M H / Sturek, Michael / Alloosh, Mouhamad / Belsham, Graham J

    Frontiers in microbiology

    2020  Volume 11, Page(s) 573756

    Abstract: The ongoing COVID-19 pandemic caused by infection with SARS-CoV-2 has created an urgent need for animal models to enable study of basic infection and disease mechanisms and for development of vaccines, therapeutics, and diagnostics. Most research on ... ...

    Abstract The ongoing COVID-19 pandemic caused by infection with SARS-CoV-2 has created an urgent need for animal models to enable study of basic infection and disease mechanisms and for development of vaccines, therapeutics, and diagnostics. Most research on animal models for COVID-19 has been directed toward rodents, transgenic rodents, and non-human primates. The primary focus has been on the angiotensin-converting enzyme 2 (ACE2), which is a host cell receptor for SARS-CoV-2. Among investigated species, irrespective of ACE2 spike protein binding, only mild (or no) disease has occurred following infection with SARS-CoV-2, suggesting that ACE2 may be necessary for infection but is not sufficient to determine the outcome of infection. The common trait of all species investigated as COVID models is their healthy status prior to virus challenge. In contrast, the vast majority of severe COVID-19 cases occur in people with chronic comorbidities such as diabetes, obesity, and/or cardiovascular disease. Healthy pigs express ACE2 protein that binds the viral spike protein but they are not susceptible to infection with SARS-CoV-2. However, certain pig breeds, such as the Ossabaw pig, can reproducibly be made obese and show most aspects of the metabolic syndrome, thus resembling the more than 80% of the critically ill COVID-19 patients admitted to hospitals. We urge considering infection with porcine respiratory coronavirus of metabolic syndrome pigs, such as the obese Ossabaw pig, as a highly relevant animal model of severe COVID-19.
    Keywords covid19
    Language English
    Publishing date 2020-09-25
    Publishing country Switzerland
    Document type Journal Article ; Review
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.573756
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  6. Article ; Online: Highly sensitive lipid detection and localization in atherosclerotic plaque with a dual-frequency intravascular photoacoustic/ultrasound catheter.

    Cao, Yingchun / Alloosh, Mouhamad / Sturek, Michael / Cheng, Ji-Xin

    Translational biophotonics

    2020  Volume 2, Issue 3

    Abstract: Intravascular photoacoustic/ultrasound (IVPA/US) is an emerging hybrid imaging modality that provides specific lipid detection and localization, while maintaining co-registered artery morphology, for diagnosis of vulnerable plaque in cardiovascular ... ...

    Abstract Intravascular photoacoustic/ultrasound (IVPA/US) is an emerging hybrid imaging modality that provides specific lipid detection and localization, while maintaining co-registered artery morphology, for diagnosis of vulnerable plaque in cardiovascular disease. However, current IVPA/US approaches based on a single-element transducer exhibit compromised performance for lipid detection due to the relatively low contrast of lipid absorption and conflicting detection bands for photoacoustic and ultrasound signals. Here, we present a dual-frequency IVPA/US catheter for highly sensitive detection and precision localization of lipids. The low frequency transducer provides enhanced photoacoustic sensitivity, while the high frequency transducer maintains state-of-the-art spatial resolution for ultrasound imaging. The boosted capability of IVPA/US imaging enables a multi-scale analysis of lipid distribution in swine with coronary atherosclerosis. The dual-frequency IVPA/US catheter has a diameter of 1 mm and flexibility to easily adapt to current catheterization procedures and is a significant step toward clinical diagnosis of vulnerable plaque.
    Language English
    Publishing date 2020-05-12
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2952354-0
    ISSN 2627-1850 ; 2627-1850
    ISSN (online) 2627-1850
    ISSN 2627-1850
    DOI 10.1002/tbio.202000004
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  7. Article ; Online: Non-responsiveness to cardioprotection by ischaemic preconditioning in Ossabaw minipigs with genetic predisposition to, but without the phenotype of the metabolic syndrome.

    Kleinbongard, Petra / Lieder, Helmut Raphael / Skyschally, Andreas / Alloosh, Mouhamad / Gödecke, Axel / Rahmann, Sven / Sturek, Michael / Heusch, Gerd

    Basic research in cardiology

    2022  Volume 117, Issue 1, Page(s) 58

    Abstract: The translation of successful preclinical and clinical proof-of-concept studies on cardioprotection to the benefit of patients with reperfused acute myocardial infarction has been difficult so far. This difficulty has been attributed to confounders which ...

    Abstract The translation of successful preclinical and clinical proof-of-concept studies on cardioprotection to the benefit of patients with reperfused acute myocardial infarction has been difficult so far. This difficulty has been attributed to confounders which patients with myocardial infarction typically have but experimental animals usually not have. The metabolic syndrome is a typical confounder. We hypothesised that there may also be a genuine non-responsiveness to cardioprotection and used Ossabaw minipigs which have the genetic predisposition to develop a diet-induced metabolic syndrome, but before they had developed the diseased phenotype. Using a prospective study design, a reperfused acute myocardial infarction was induced in 62 lean Ossabaw minipigs by 60 min coronary occlusion and 180 min reperfusion. Ischaemic preconditioning by 3 cycles of 5 min coronary occlusion and 10 min reperfusion was used as cardioprotective intervention. Ossabaw minipigs were stratified for their single nucleotide polymorphism as homozygous for valine (V/V) or isoleucine (I/I)) in the γ-subunit of adenosine monophosphate-activated protein kinase. Endpoints were infarct size and area of no-reflow. Infarct size (V/V: 54 ± 8, I/I: 54 ± 13% of area at risk, respectively) was not reduced by ischaemic preconditioning (V/V: 55 ± 11, I/I: 46 ± 11%) nor was the area of no-reflow (V/V: 57 ± 18, I/I: 49 ± 21 vs. V/V: 57 ± 21, I/I: 47 ± 21% of infarct size). Bioinformatic comparison of the Ossabaw genome to that of Sus scrofa and Göttingen minipigs identified differences in clusters of genes encoding mitochondrial and inflammatory proteins, including the janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway. The phosphorylation of STAT3 at early reperfusion was not increased by ischaemic preconditioning, different from the established STAT3 activation by cardioprotective interventions in other pig strains. Ossabaw pigs have not only the genetic predisposition to develop a metabolic syndrome but also are not amenable to cardioprotection by ischaemic preconditioning.
    Language English
    Publishing date 2022-11-11
    Publishing country Germany
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't ; Comment
    ZDB-ID 189755-x
    ISSN 1435-1803 ; 0300-8428 ; 0175-9418
    ISSN (online) 1435-1803
    ISSN 0300-8428 ; 0175-9418
    DOI 10.1007/s00395-022-00965-0
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  8. Article ; Online: Insulin-Dendrimer Nanocomplex for Multi-Day Glucose-Responsive Therapy in Mice and Swine.

    Xian, Sijie / Xiang, Yuanhui / Liu, Dongping / Fan, Bowen / Mitrová, Katarína / Ollier, Rachel C / Su, Bo / Alloosh, Muhammad Ali / Jiráček, Jiří / Sturek, Michael / Alloosh, Mouhamad / Webber, Matthew J

    Advanced materials (Deerfield Beach, Fla.)

    2023  Volume 36, Issue 5, Page(s) e2308965

    Abstract: The management of diabetes in a manner offering autonomous insulin therapy responsive to glucose-directed need, and moreover with a dosing schedule amenable to facile administration, remains an ongoing goal to improve the standard of care. While basal ... ...

    Abstract The management of diabetes in a manner offering autonomous insulin therapy responsive to glucose-directed need, and moreover with a dosing schedule amenable to facile administration, remains an ongoing goal to improve the standard of care. While basal insulins with reduced dosing frequency, even once-weekly administration, are on the horizon, there is still no approved therapy that offers glucose-responsive insulin function. Herein, a nanoscale complex combining both electrostatic- and dynamic-covalent interactions between a synthetic dendrimer carrier and an insulin analogue modified with a high-affinity glucose-binding motif yields an injectable insulin depot affording both glucose-directed and long-lasting insulin availability. Following a single injection, it is even possible to control blood glucose for at least one week in diabetic swine subjected to daily oral glucose challenges. Measurements of serum insulin concentration in response to challenge show increases in insulin corresponding to elevated blood glucose levels, an uncommon finding even in preclinical work on glucose-responsive insulin. Accordingly, the subcutaneous nanocomplex that results from combining electrostatic- and dynamic-covalent interactions between a modified insulin and a synthetic dendrimer carrier affords a glucose-responsive insulin depot for week-long control following a single routine injection.
    MeSH term(s) Swine ; Animals ; Mice ; Insulin ; Glucose ; Blood Glucose ; Dendrimers ; Diabetes Mellitus
    Chemical Substances Insulin ; Glucose (IY9XDZ35W2) ; Blood Glucose ; Dendrimers
    Language English
    Publishing date 2023-12-03
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1474949-X
    ISSN 1521-4095 ; 0935-9648
    ISSN (online) 1521-4095
    ISSN 0935-9648
    DOI 10.1002/adma.202308965
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  9. Article ; Online: Intracellular Ca

    Badin, Jill K / Eggenberger, Caleb / Rodenbeck, Stacey Dineen / Hashmi, Zubair A / Wang, I-Wen / Garcia, Jose P / Alloosh, Mouhamad / Sturek, Michael

    Journal of cardiovascular translational research

    2021  Volume 15, Issue 1, Page(s) 167–178

    Abstract: Intracellular free ... ...

    Abstract Intracellular free Ca
    MeSH term(s) Animals ; Calcium/metabolism ; Coronary Artery Disease/metabolism ; Coronary Vessels/metabolism ; Humans ; Metabolic Syndrome/metabolism ; Muscle, Smooth/metabolism ; Swine ; Swine, Miniature/metabolism
    Chemical Substances Calcium (SY7Q814VUP)
    Language English
    Publishing date 2021-07-20
    Publishing country United States
    Document type Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't
    ZDB-ID 2422411-X
    ISSN 1937-5395 ; 1937-5387
    ISSN (online) 1937-5395
    ISSN 1937-5387
    DOI 10.1007/s12265-021-10153-5
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  10. Article ; Online: Animal Models for COVID-19

    Heegaard, Peter M. H. / Sturek, Michael / Alloosh, Mouhamad / Belsham, Graham J.

    Frontiers in Microbiology

    More to the Picture Than ACE2, Rodents, Ferrets, and Non-human Primates. A Case for Porcine Respiratory Coronavirus and the Obese Ossabaw Pig

    2020  Volume 11

    Keywords Microbiology (medical) ; Microbiology ; covid19
    Publisher Frontiers Media SA
    Publishing country ch
    Document type Article ; Online
    ZDB-ID 2587354-4
    ISSN 1664-302X
    ISSN 1664-302X
    DOI 10.3389/fmicb.2020.573756
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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