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  1. AU="Alonso, Luis L"
  2. AU="Weaver, Matthew D"

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  1. Article ; Online: Studying Venom Toxin Variation Using Accurate Masses from Liquid Chromatography-Mass Spectrometry Coupled with Bioinformatic Tools.

    Alonso, Luis L / van Thiel, Jory / Slagboom, Julien / Dunstan, Nathan / Modahl, Cassandra M / Jackson, Timothy N W / Samanipour, Saer / Kool, Jeroen

    Toxins

    2024  Volume 16, Issue 4

    Abstract: This study provides a new methodology for the rapid analysis of numerous venom samples in an automated fashion. Here, we use LC-MS (Liquid Chromatography-Mass Spectrometry) for venom separation and toxin analysis at the accurate mass level combined with ... ...

    Abstract This study provides a new methodology for the rapid analysis of numerous venom samples in an automated fashion. Here, we use LC-MS (Liquid Chromatography-Mass Spectrometry) for venom separation and toxin analysis at the accurate mass level combined with new in-house written bioinformatic scripts to obtain high-throughput results. This analytical methodology was validated using 31 venoms from all members of a monophyletic clade of Australian elapids: brown snakes (
    MeSH term(s) Animals ; Computational Biology ; Elapid Venoms/chemistry ; Elapid Venoms/analysis ; Elapidae ; Liquid Chromatography-Mass Spectrometry
    Chemical Substances Elapid Venoms
    Language English
    Publishing date 2024-04-07
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins16040181
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Metabolome-Based Classification of Snake Venoms by Bioinformatic Tools.

    Alonso, Luis L / Slagboom, Julien / Casewell, Nicholas R / Samanipour, Saer / Kool, Jeroen

    Toxins

    2023  Volume 15, Issue 2

    Abstract: Snakebite is considered a neglected tropical disease, and it is one of the most intricate ones. The variability found in snake venom is what makes it immensely complex to study. These variations are present both in the big and the small molecules found ... ...

    Abstract Snakebite is considered a neglected tropical disease, and it is one of the most intricate ones. The variability found in snake venom is what makes it immensely complex to study. These variations are present both in the big and the small molecules found in snake venom. This study focused on examining the variability found in the venom's small molecules (i.e., mass range of 100-1000 Da) between two main families of venomous snakes-Elapidae and Viperidae-managing to create a model able to classify unknown samples by means of specific features, which can be extracted from their LC-MS data and output in a comprehensive list. The developed model also allowed further insight into the composition of snake venom by highlighting the most relevant metabolites of each group by clustering similarly composed venoms. The model was created by means of support vector machines and used 20 features, which were merged into 10 principal components. All samples from the first and second validation data subsets were correctly classified. Biological hypotheses relevant to the variation regarding the metabolites that were identified are also given.
    MeSH term(s) Animals ; Humans ; Snake Venoms ; Elapidae/metabolism ; Viperidae/metabolism ; Snake Bites ; Mass Spectrometry ; Elapid Venoms/metabolism
    Chemical Substances Snake Venoms ; Elapid Venoms
    Language English
    Publishing date 2023-02-15
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins15020161
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  3. Article ; Online: Highly Evolvable: Investigating Interspecific and Intraspecific Venom Variation in Taipans (

    van Thiel, Jory / Alonso, Luis L / Slagboom, Julien / Dunstan, Nathan / Wouters, Roel M / Modahl, Cassandra M / Vonk, Freek J / Jackson, Timothy N W / Kool, Jeroen

    Toxins

    2023  Volume 15, Issue 1

    Abstract: Snake venoms are complex mixtures of toxins that differ on interspecific (between species) and intraspecific (within species) levels. Whether venom variation within a group of closely related species is explained by the presence, absence and/or relative ... ...

    Abstract Snake venoms are complex mixtures of toxins that differ on interspecific (between species) and intraspecific (within species) levels. Whether venom variation within a group of closely related species is explained by the presence, absence and/or relative abundances of venom toxins remains largely unknown. Taipans (
    MeSH term(s) Animals ; Elapid Venoms/genetics ; Phylogeny ; Elapidae/genetics ; Snake Bites ; Snake Venoms ; Snakes ; Toxins, Biological ; Antivenins
    Chemical Substances Elapid Venoms ; Snake Venoms ; Toxins, Biological ; Antivenins
    Language English
    Publishing date 2023-01-13
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins15010074
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article ; Online: Application of an Extracellular Matrix-Mimicking Fluorescent Polymer for the Detection of Proteolytic Venom Toxins.

    Wachtel, Eric / Bittenbinder, Matyas A / van de Velde, Bas / Slagboom, Julien / de Monts de Savasse, Axel / Alonso, Luis L / Casewell, Nicholas R / Vonk, Freek J / Kool, Jeroen

    Toxins

    2023  Volume 15, Issue 4

    Abstract: The cytotoxicity caused by snake venoms is a serious medical problem that greatly contributes to the morbidity observed in snakebite patients. The cytotoxic components found in snake venoms belong to a variety of toxin classes and may cause cytotoxic ... ...

    Abstract The cytotoxicity caused by snake venoms is a serious medical problem that greatly contributes to the morbidity observed in snakebite patients. The cytotoxic components found in snake venoms belong to a variety of toxin classes and may cause cytotoxic effects by targeting a range of molecular structures, including cellular membranes, the extracellular matrix (ECM) and the cytoskeleton. Here, we present a high-throughput assay (384-well plate) that monitors ECM degradation by snake venom toxins via the application of fluorescent versions of model ECM substrates, specifically gelatin and collagen type I. Both crude venoms and fractionated toxins of a selection of medically relevant viperid and elapid species, separated via size-exclusion chromatography, were studied using the self-quenching, fluorescently labelled ECM-polymer substrates. The viperid venoms showed significantly higher proteolytic degradation when compared to elapid venoms, although the venoms with higher snake venom metalloproteinase content did not necessarily exhibit stronger substrate degradation than those with a lower one. Gelatin was generally more readily cleaved than collagen type I. In the viperid venoms, which were subjected to fractionation by SEC, two (
    MeSH term(s) Humans ; Collagen Type I ; Gelatin ; Snake Venoms/chemistry ; Toxins, Biological ; Snake Bites ; Elapid Venoms/chemistry ; Metalloproteases ; Extracellular Matrix
    Chemical Substances Collagen Type I ; Gelatin (9000-70-8) ; Snake Venoms ; Toxins, Biological ; Elapid Venoms ; Metalloproteases (EC 3.4.-)
    Language English
    Publishing date 2023-04-18
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2518395-3
    ISSN 2072-6651 ; 2072-6651
    ISSN (online) 2072-6651
    ISSN 2072-6651
    DOI 10.3390/toxins15040294
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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