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Article ; Online: Molecular detection and genetic characterization of Trichomonas gallinae in falcons in Saudi Arabia.

Alrefaei, Abdulwahed Fahad

PloS one

2020 Volume 15, Issue 10, Page(s) e0241411

Abstract: Avian trichomonosis is primarily caused by Trichomonas gallinae, a flagellated protozoan parasite that especially infects the upper digestive tract of columbid bird species and their avian predators. However, this parasite has recently been found to be ... ...

Abstract	Avian trichomonosis is primarily caused by Trichomonas gallinae, a flagellated protozoan parasite that especially infects the upper digestive tract of columbid bird species and their avian predators. However, this parasite has recently been found to be distributed worldwide in various other avian species. This parasitic disease is common in captive falcons in Saudi Arabia and the Middle East. This study aimed to examine and identify the genetic variation of T. gallinae obtained from three species of falcons in Saudi Arabia via the sequencing analysis of the internal transcribed spacer (ITS) region. Swab samples from 97 saker falcons (Falco cherrug), 24 peregrine falcons (Falco peregrinus) and 37 gyrfalcons (Falco rusticolus) were cultured and analysed for infection between 2018 and 2019. The overall prevalence of infection by T. gallinae was 26.58% (n = 42), of which 35 (83.33%) were collected from Riyadh region and seven (16.67%) were collected from Qassim region. The results indicate the presence of four genotypes of T. gallinae in Saudi falcons: A, C, II, and KSA11. This study reports for the first time genetic diversity of T. gallinae in these falcons in Saudi Arabia.
MeSH term(s)	Animals ; DNA, Intergenic/genetics ; Falconiformes/parasitology ; Polymerase Chain Reaction ; Saudi Arabia ; Trichomonas/genetics ; Trichomonas/physiology
Chemical Substances	DNA, Intergenic
Language	English
Publishing date	2020-10-29
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1932-6203
ISSN (online)	1932-6203
DOI	10.1371/journal.pone.0241411
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Article ; Online: In vitro analysis of novel trimetallic (Ag-Cu-Ni TNC) to handle benzene and benzopyrene pollutants in an aqueous environment.

Jhanani, G K / Albeshr, Mohammed F / Alrefaei, Abdulwahed Fahad

Chemosphere

2023 Volume 343, Page(s) 140075

Abstract: In this study, a trimetallic nanocomposite comprising Silver Copper-Nickel (Ag-Cu-Ni TNC) was synthesized and analysed for its efficiency in degrading benzene and benzopyrene, which has five fused benzene rings. Fabrication of trimetallic nanocomposites ... ...

Abstract	In this study, a trimetallic nanocomposite comprising Silver Copper-Nickel (Ag-Cu-Ni TNC) was synthesized and analysed for its efficiency in degrading benzene and benzopyrene, which has five fused benzene rings. Fabrication of trimetallic nanocomposites were characterized using UV spectroscopy, FTIR studies, SEM EDAX, and DLS results. XRD confirmed the cubic crystalline Fcc structure of Ag-Cu-Ni TNC. Photocatalytic degradation analysis revealed that Ag-Cu-Ni TNC has the efficient photocatalytic ability, and the optimum condition required for efficient degradation of benzene and benzopyrene was identified as 2 μg/mL of PAH molecule, 10 μg/mL of Ag-Cu-Ni TNC at pH 5, stirring time of 2 h placed under UV light. Based on these optimum conditions, kinetic and isotherm studies were performed, revealing that the adsorption of benzene and benzopyrene by Ag-Cu-Ni TNC fits well with the Pseudo-second order kinetic model and Freundlich isotherm model. Thus, our study's adsorption of PAH molecule from aqueous solution takes place through chemisorption and involves heterogeneous adsorption phenomena.
Language	English
Publishing date	2023-09-05
Publishing country	England
Document type	Journal Article
ZDB-ID	120089-6
ISSN	1879-1298 ; 0045-6535 ; 0366-7111
ISSN (online)	1879-1298
ISSN	0045-6535 ; 0366-7111
DOI	10.1016/j.chemosphere.2023.140075
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Article ; Online: Comparison of the SARS-CoV-2 (2019-nCoV) M protein with its counterparts of SARS-CoV and MERS-CoV species.

Alharbi, Sultan Nafea / Alrefaei, Abdulwahed Fahad

Journal of King Saud University. Science

2021 Volume 33, Issue 2, Page(s) 101335

Abstract: ... ...

Abstract	Coronaviruses
Language	English
Publishing date	2021-01-07
Publishing country	Saudi Arabia
Document type	Journal Article
ISSN	2213-686X
ISSN (online)	2213-686X
DOI	10.1016/j.jksus.2020.101335
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Article ; Online: Interaction of blood calcium with milk yield, energy metabolites, subclinical mastitis, and reproductive performance in crossbred dairy cows.

Khan, Shah Murad / Khan, Rifat Ullah / Naz, Shabana / Alrefaei, Abdulwahed Fahad / Tufarelli, Vincenzo

Reproduction in domestic animals = Zuchthygiene

2024 Volume 59, Issue 4, Page(s) e14557

Abstract: Limited literature is available on the consequences of postpartum low blood calcium (Ca) concentration in crossbred cows. The research aimed to investigate the correlation between postpartum serum Ca levels and various parameters, including milk yield, ... ...

Abstract	Limited literature is available on the consequences of postpartum low blood calcium (Ca) concentration in crossbred cows. The research aimed to investigate the correlation between postpartum serum Ca levels and various parameters, including milk yield, serum energy metabolites, milk somatic cell count, and reproductive factors in crossbred cows. Following parturition, a total of 45 potential high-yielding F2 (HF × Sahiwal; Genotype: 75:25) dairy cows were enrolled . These cows were categorized based on plasma calcium concentrations into three groups: a low calcium group (Ca-L) with a calcium concentration of <5 mg/dL, a medium calcium group (Ca-M) with a calcium concentration ranging from 5 to 8.5 mg/dL, and a high calcium group (Ca-H) with a calcium concentration exceeding 8.5 mg/dL. The study parameters were measured over an 8-week period. The results indicated that overall milk yield and blood glucose were significantly higher in the Ca-H group compared to Ca-M and Ca-L (p < .01). Blood cholesterol was significantly higher in Ca-M (p < .01), while blood triglyceride was significantly lower in both Ca-M and Ca-H. Overall, blood cortisol did not show a significant change between these groups (p < .01); however, progesterone levels were higher (p < .01) in Ca-M and Ca-H cows. Furthermore, somatic cell count (SCC) significantly (p < .01) decreased in cows with Ca-H compared to Ca-L. Additionally, postpartum oestrous interval and interestrus interval decreased significantly (p < .01) in Ca-M and Ca-H compared to Ca-L. These findings suggest that cows with blood calcium levels exceeding 8.5 mg/dL exhibited significantly higher milk yield, blood metabolite levels, a lower likelihood of subclinical mastitis, and earlier reproductive activity after calving.
MeSH term(s)	Female ; Cattle ; Animals ; Pregnancy ; Calcium ; Milk ; Mastitis, Bovine ; Hematologic Tests/veterinary ; Cattle Diseases
Chemical Substances	Calcium (SY7Q814VUP)
Language	English
Publishing date	2024-04-13
Publishing country	Germany
Document type	Journal Article
ZDB-ID	1015187-4
ISSN	1439-0531 ; 0936-6768
ISSN (online)	1439-0531
ISSN	0936-6768
DOI	10.1111/rda.14557
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Article ; Online: Ribosomal DNA Sequence-Based Taxonomy and Antimicrobial Activity of

Mussai, Prakash / Larsen, Jacob / Alrefaei, Abdulwahed Fahad / Jeewon, Rajesh

Marine drugs

2023 Volume 21, Issue 4

Abstract: Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains ... ...

Abstract	Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of
MeSH term(s)	Humans ; DNA, Ribosomal/genetics ; Phylogeny ; Ecosystem ; Base Sequence ; Indian Ocean ; Mauritius ; Anti-Infective Agents/pharmacology ; Dinoflagellida
Chemical Substances	DNA, Ribosomal ; Anti-Infective Agents
Language	English
Publishing date	2023-03-28
Publishing country	Switzerland
Document type	Journal Article
ZDB-ID	2175190-0
ISSN	1660-3397 ; 1660-3397
ISSN (online)	1660-3397
ISSN	1660-3397
DOI	10.3390/md21040216
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Article ; Online: Ribosomal DNA Sequence-Based Taxonomy and Antimicrobial Activity of Prorocentrum spp. (Dinophyceae) from Mauritius Coastal Waters, South-West Indian Ocean

Mussai, Prakash / Larsen, Jacob / Alrefaei, Abdulwahed Fahad / Jeewon, Rajesh

Mar Drugs. 2023 Mar. 28, v. 21, no. 4

2023

Abstract: Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of Prorocentrum species were isolated from macrophytes and cultured under standard laboratory ... ...

Abstract	Microalgae are unicellular organisms and commonly present in the euphotic zone of marine ecosystems. From the western coast of Mauritius, three strains of Prorocentrum species were isolated from macrophytes and cultured under standard laboratory conditions. Morphologies were examined by light, fluorescence, and scanning electron microscopy, and phylogenetic analyses were based on partial large subunit LSU rDNA (D1-D2) and ITS1-5.8S-ITS2 (ITS) regions. Three Prorocentrum species, including the P. fukuyoi complex, P. rhathymum, and P. lima complex, were identified. The antimicrobial activities were assayed against potential human pathogenic bacterial strains. The highest zone of inhibition was recorded for intracellular and extracellular protein extracts of Prorocentrum rhathymum against Vibrio parahaemolyticus. The polysaccharide extracts of the Prorocentrum fukuyoi complex had a higher zone of inhibition (24 ± 0.4 mm) against MRSA at a minimum concentration of 0.625 μg/mL. The extracts from the three Prorocentrum species had different levels of activity against the pathogens used, and this can be of scientific interest in the search for antibiotics from natural marine sources.
Keywords	Prorocentrum ; Vibrio parahaemolyticus ; antimicrobial properties ; coastal water ; coasts ; electron microscopy ; euphotic zone ; fluorescence ; macrophytes ; methicillin-resistant Staphylococcus aureus ; microalgae ; pathogens ; phylogeny ; polysaccharides ; ribosomal DNA ; taxonomy ; Indian Ocean ; Mauritius
Language	English
Dates of publication	2023-0328
Publishing place	Multidisciplinary Digital Publishing Institute
Document type	Article ; Online
ZDB-ID	2175190-0
ISSN	1660-3397
ISSN	1660-3397
DOI	10.3390/md21040216
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Role of mycorrhizas and root exudates in plant uptake of soil nutrients (calcium, iron, magnesium, and potassium): has the puzzle been completely solved?

Sardans, Jordi / Lambers, H. / Preece, Catherine / Alrefaei, Abdulwahed Fahad / Penuelas, Josep

The Plant Journal. 2023 June, v. 114, no. 6 p.1227-1242

2023

Abstract: Anthropogenic global change is driving an increase in the frequency and intensity of drought and flood events, along with associated imbalances and limitation of several soil nutrients. In the context of an increasing human population, these impacts ... ...

Abstract	Anthropogenic global change is driving an increase in the frequency and intensity of drought and flood events, along with associated imbalances and limitation of several soil nutrients. In the context of an increasing human population, these impacts represent a global‐scale challenge for biodiversity conservation and sustainable crop production to ensure food security. Plants have evolved strategies to enhance uptake of soil nutrients under environmental stress conditions; for example, symbioses with fungi (mycorrhization) in the rhizosphere and the release of exudates from roots. Although crop cultivation is managed for the effects of limited availability of nitrogen (N) and phosphorus (P), there is increasing evidence for limitation of plant growth and fitness because of the low availability of other soil nutrients such as the metals potassium (K), calcium (Ca), magnesium (Mg), and iron (Fe), which may become increasingly limiting for plant productivity under global change. The roles of mycorrhizas and plant exudates on N and P uptake have been studied intensively; however, our understanding of the effects on metal nutrients is less clear and still inconsistent. Here, we review the literature on the role of mycorrhizas and root exudates in plant uptake of key nutrients (N, P, K, Ca, Mg, and Fe) in the context of potential nutrient deficiencies in crop and non‐crop terrestrial ecosystems, and identify knowledge gaps for future research to improve nutrient‐uptake capacity in food crop plants.
Keywords	biodiversity conservation ; calcium ; drought ; food crops ; food security ; global change ; human population ; iron ; magnesium ; mycorrhizae ; nitrogen ; nutrient uptake ; phosphorus ; plant growth ; potassium ; rhizosphere ; soil ; sustainable agriculture
Language	English
Dates of publication	2023-06
Size	p. 1227-1242.
Publishing place	John Wiley & Sons, Ltd
Document type	Article ; Online
Note	REVIEW
ZDB-ID	1088037-9
ISSN	1365-313X ; 0960-7412
ISSN (online)	1365-313X
ISSN	0960-7412
DOI	10.1111/tpj.16184
Database	NAL-Catalogue (AGRICOLA)

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Article ; Online: Impact of coriander (

Hafeez, Abdul / Shahid Ali, Said / Akhtar, Junaid / Naz, Shabana / Alrefaei, Abdulwahed Fahad / Albeshr, Mohammed Fahad / Israr, Muhammad / Ullah Khan, Rifat

The veterinary quarterly

2024 Volume 44, Issue 1, Page(s) 1–7

Abstract: The study investigated the impact of incorporating a specific herbal blend comprising coriander, garlic, and fenugreek (CGF) at various levels on the zootechnical performance, blood metabolites and nutrient digestibility in broiler chickens. The 42-day ... ...

Abstract	The study investigated the impact of incorporating a specific herbal blend comprising coriander, garlic, and fenugreek (CGF) at various levels on the zootechnical performance, blood metabolites and nutrient digestibility in broiler chickens. The 42-day experiment involved 360 broilers (Cobb 500), organized into four distinct treatment groups. The dietary interventions included a control group consisting of a basal diet and the same diet was supplemented with CGF at rates of 1, 2, and 3%. Broilers receiving a 1% phytogenic mixture exhibited significantly increased live weight and carcass weight. Moreover, the digestibility of crude protein and crude fat significantly improved in broilers supplemented with a 1% phytogenic mixture. On the other hand, the digestibility of calcium and phosphorus showed a notable increase in broilers fed with a 3% phytogenic mixture. Regarding serum metabolites, the 1% phytogenic mixture group displayed significantly higher levels of high density lipoprotein and triglycerides. The supplementation of the broiler diet with a herbal mixture of coriander, fenugreek, and garlic at a 1% rate resulted in improved growth performance, carcass quality, nutrient digestion, and lipid profile.
MeSH term(s)	Animals ; Garlic ; Coriandrum ; Chickens ; Trigonella ; Nutrients
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Journal Article
ZDB-ID	754161-2
ISSN	1875-5941 ; 0165-2176
ISSN (online)	1875-5941
ISSN	0165-2176
DOI	10.1080/01652176.2023.2300948
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Article ; Online: Boosting Solanum tuberosum resistance to Alternaria solani through green synthesized ferric oxide (Fe

Anwaar, Sadaf / Ijaz, Dur-E-Shahwar / Anwar, Tauseef / Qureshi, Huma / Nazish, Moona / Alrefaei, Abdulwahed Fahad / Almutairi, Mikhlid H / Alharbi, Sultan N

Scientific reports

2024 Volume 14, Issue 1, Page(s) 2375

Abstract: Potato (Solanum tuberosum) is the third crucial global crop facing threats from Alternaria solani, a necrotrophic fungal pathogen causing early blight disease. Beyond crop impact, it leads to substantial production reduction and economic losses worldwide. ...

Abstract	Potato (Solanum tuberosum) is the third crucial global crop facing threats from Alternaria solani, a necrotrophic fungal pathogen causing early blight disease. Beyond crop impact, it leads to substantial production reduction and economic losses worldwide. This study introduces a green synthesis method for producing Ferric Oxide nanoparticles (FNPs) using dried Guava (Psidium guajava) leaves. Guava leaf extract acts as a reducing agent, with iron (III) chloride hexahydrate (FeCl
MeSH term(s)	Solanum tuberosum ; Nanoparticles/chemistry ; Ferric Compounds/chemistry ; Spectroscopy, Fourier Transform Infrared ; Metal Nanoparticles/chemistry ; Plant Extracts/chemistry ; X-Ray Diffraction ; Anti-Bacterial Agents/chemistry ; Alternaria
Chemical Substances	ferric oxide (1K09F3G675) ; Ferric Compounds ; Plant Extracts ; Anti-Bacterial Agents
Language	English
Publishing date	2024-01-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-024-52704-1
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Article: Multigene phylogeny, bioactive properties, enzymatic and dye decolorization potential of selected marine fungi from brown algae and sponges of Mauritius.

Wong Chin, Jessica Mélanie / Puchooa, Daneshwar / Bahorun, Theeshan / Alrefaei, Abdulwahed Fahad / Neergheen, Vidushi S / Jeewon, Rajesh

Heliyon

2024 Volume 10, Issue 7, Page(s) e28955

Abstract: Marine fungi represent an important proportion of the microbial diversity in the oceans. They are attractive candidates for biotechnological purposes and industrial applications. Despite an increasing interest in mycology, marine fungi associated with ... ...

Abstract	Marine fungi represent an important proportion of the microbial diversity in the oceans. They are attractive candidates for biotechnological purposes and industrial applications. Despite an increasing interest in mycology, marine fungi associated with sponges and algae have been poorly studied in Mauritius. The objectives of this study were to: 1) use multigene phylogenetic analyses to identify isolated marine fungi; 2) determine the differences in the antimicrobial and antioxidant properties of the fungal extracts; and 3) assess their enzyme activities and dye decolorization potential. Five fungal isolates
Language	English
Publishing date	2024-04-02
Publishing country	England
Document type	Journal Article
ZDB-ID	2835763-2
ISSN	2405-8440
ISSN	2405-8440
DOI	10.1016/j.heliyon.2024.e28955
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